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一株草苷膦极端抗性菌株的分离和分子鉴定 被引量:8
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作者 刘柱 陈明 +3 位作者 徐玉泉 陆伟 杨志荣 林敏 《高技术通讯》 EI CAS CSCD 2002年第12期25-28,共4页
从极端污染的土壤中 ,分离到一株对草苷膦有极端抗性的细菌菌株HTG7。它能够在以草苷膦为选择压力的限制性培养基上生长 ,其最高耐受能力为 5 0 0mmol/L。采用PCR扩增获得该菌 16SrDNA片段。核苷酸序列分析表明 ,该菌的 16SrDNA核苷酸... 从极端污染的土壤中 ,分离到一株对草苷膦有极端抗性的细菌菌株HTG7。它能够在以草苷膦为选择压力的限制性培养基上生长 ,其最高耐受能力为 5 0 0mmol/L。采用PCR扩增获得该菌 16SrDNA片段。核苷酸序列分析表明 ,该菌的 16SrDNA核苷酸序列与盐单胞菌属同源性最高 ,在 95 %~ 98%之间。在细菌系统分类学上 ,HTG7归属盐单胞菌属Variabil。 展开更多
关键词 极端抗性 菌株 分离 分子鉴定 草甘膦 16SrDNA 除草剂 机制
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Direct tension and fracture resistance curves of ultra high performance marine composite materials
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作者 吴香国 韩相默 《Journal of Marine Science and Application》 2008年第3期218-225,共8页
Fracture behavior is one of the most important,yet still little understood properties of ultra-high performance cementitious composites(UHPCC),a new marine structural engineering material. Research on the fracture and... Fracture behavior is one of the most important,yet still little understood properties of ultra-high performance cementitious composites(UHPCC),a new marine structural engineering material. Research on the fracture and direct tension behavior of UHPCC was carried out.The constitution law of UHPCC was divided into three phases:pre-partial debonding,partial debonding,and pullout phases.A direct tension constitution law was constructed based on the proposed fiber reinforcing parameter as a function of fiber volume fraction,fiber diameter and length,and fiber bonding strength.With the definition of linear crack shape,the energy release rate of UHPCC was derived and the R-curve equation was calculated from this.Loading tests of UHPCC using a three-point bending beam with an initial notch were carried out.The predictions from the proposed R-curve were in good agreement with the test results, indicating that the proposed R-curve accurately describes the fracture resistance of UHPCC.Introduction of a fiber reinforcement parameter bridges the fracture property R-curve and micro-composites’ mechanics parameters together.This has laid the foundation for further research into fracture properties based on micro-mechanics.The proposed tension constitution law and R-curve can be references for future UHPCC fracture evaluation. 展开更多
关键词 ultra high performance cementitious composites FRACTURE resistance curve direct tension
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