In this report, we describe the isolation and characterization of microsatellite loci for forest musk deer （Moschus berezovskii） through an improved enrichment protocol. Five new markers were isolated from the genom...In this report, we describe the isolation and characterization of microsatellite loci for forest musk deer （Moschus berezovskii） through an improved enrichment protocol. Five new markers were isolated from the genomic DNA of forest musk deer and showed high polymorphism with 4 - 13 alleles in 24 sampled individuals from the population of Jinfeng Mountain, Sichuan Province, China. The observed and expected heterozygesities were from 0.429 - 0.957 and 0.587 - 0.902, respectively. The average polymorphism information content （PIC） value in these five loci was 0. 730. This suggests that the five microsatellite loci are a valuable tool for further studies about forest musk deer.展开更多
The genetic diversity and population structure of the Dybowski＇s frog（Rana dybowskii） were investigated by using 11 polymorphic microsatellite loci.Total 75 individuals were sampled from six populations in Lesser K...The genetic diversity and population structure of the Dybowski＇s frog（Rana dybowskii） were investigated by using 11 polymorphic microsatellite loci.Total 75 individuals were sampled from six populations in Lesser Khingan Mountains and Changbai Mountains,China.Results showed that allele number of the 11 microsatellite loci was in the range of 2-10 in all populations,with the mean of 5.6.The average expected heterozygosity（HE） was 0.572,indicating a moderate polymorphism.The results of genetic differentiation coefficient（FST） showed that population genetic differentiation was significant between Changbai and Lesser Khingan Mountains（p〈0.001）.This result was verified further by Nei＇s genetic distance（DA） based on UPGMA phy-logenetic trees and by AMOVA analysis.In conclusion,the populations distributed in Lesser Khingan Mountains and Changbai Mountain are proposed to be two distinct management units（MUs） for their protection and management.展开更多
With the help of GIS tool of ARC/INFO, ARCVIEW and FRAGSTATS, the map of forest resource distribution of Heilongjiang Province was analyzed in 1896, 1949 and 1981. Using total area, mean patch size, patch density, coe...With the help of GIS tool of ARC/INFO, ARCVIEW and FRAGSTATS, the map of forest resource distribution of Heilongjiang Province was analyzed in 1896, 1949 and 1981. Using total area, mean patch size, patch density, coefficient of patch size variation, mean patch fractal dimension and mean shape index, we studied the change of forest landscape pattern and the change of each patch types in this region. As a result, the total area of forest landscape and mean patch size decreased sharply, the quantity and density of patches increased, the juxtaposition of patches weakened, the shape of patch tended to become regular, and the border of patch simplified. All these showed that the forest landscape of this area tended to fragment gradually, and the fragment of Korean pine forest is the severest. The diversity of whole forest landscape and the evenness of landscape types distribution reduced gradually. Human impact, instead of climate change and forest community succession, is the most important reason for such dramatic changes.展开更多
With Maolan Nature Reserve as an example, its forest landscape characteristics and diversity were studied by utilizing GIS. The results showed that the overall forest landscape in Maolan Nature Reserve was quite good,...With Maolan Nature Reserve as an example, its forest landscape characteristics and diversity were studied by utilizing GIS. The results showed that the overall forest landscape in Maolan Nature Reserve was quite good, but with the increasing natural interferences and human activities, shrub forests and non-woodland landscape types have been multiplied; among the 3 functional zones of the Nature Reserve, landscape fragmentation degree is low in core zone and buffer zone, but high in experimental zone.展开更多
[Objective] This study aimed to investigate the fungi in Wula Mountain National Forest Park. [Method] More than 180 fungal specimens were collected from Wula Mountain National Forest Park from 2009 to 2012 for primari...[Objective] This study aimed to investigate the fungi in Wula Mountain National Forest Park. [Method] More than 180 fungal specimens were collected from Wula Mountain National Forest Park from 2009 to 2012 for primarily studying the fungal species diversity. [Results] According to the classification system presented by Ainsworth et al. and with reference to the China catalogue of Macrofungi in species diversity catalogue of Fungi at Wula Mountain National Forest Park was written, involving in 80 species belonging to 43 genera, 22 families, 5 orders and 2 classes in the Basidiomycotina, and 6 species belonging to 2 genera, 2 families, 2 orders and2 classes in Ascomycotina, totally from 86 species, 45 genera, 7 orders, 24 families and 2 subdivisions. Among them, 49 species were edible and 22 species were medicinal, and 18 species were both edible and medicinal, and 7 species were poisonous, and 32 species were wood-rotting, and 5 species were mycorrhizal fungi,and 3 species were newly-recorded ones in Inner Mongolia. [Conclusion] There are still some specimens that have not been identified yet because of lack of literature,thus requiring further study for supplement.展开更多
文摘In this report, we describe the isolation and characterization of microsatellite loci for forest musk deer （Moschus berezovskii） through an improved enrichment protocol. Five new markers were isolated from the genomic DNA of forest musk deer and showed high polymorphism with 4 - 13 alleles in 24 sampled individuals from the population of Jinfeng Mountain, Sichuan Province, China. The observed and expected heterozygesities were from 0.429 - 0.957 and 0.587 - 0.902, respectively. The average polymorphism information content （PIC） value in these five loci was 0. 730. This suggests that the five microsatellite loci are a valuable tool for further studies about forest musk deer.
文摘The genetic diversity and population structure of the Dybowski＇s frog（Rana dybowskii） were investigated by using 11 polymorphic microsatellite loci.Total 75 individuals were sampled from six populations in Lesser Khingan Mountains and Changbai Mountains,China.Results showed that allele number of the 11 microsatellite loci was in the range of 2-10 in all populations,with the mean of 5.6.The average expected heterozygosity（HE） was 0.572,indicating a moderate polymorphism.The results of genetic differentiation coefficient（FST） showed that population genetic differentiation was significant between Changbai and Lesser Khingan Mountains（p〈0.001）.This result was verified further by Nei＇s genetic distance（DA） based on UPGMA phy-logenetic trees and by AMOVA analysis.In conclusion,the populations distributed in Lesser Khingan Mountains and Changbai Mountain are proposed to be two distinct management units（MUs） for their protection and management.
基金The research is supported by Study on the interaction of global change and terrestrial ecosystem in eastern China - 39899370 and the Northeast Forestry University research fund.
文摘With the help of GIS tool of ARC/INFO, ARCVIEW and FRAGSTATS, the map of forest resource distribution of Heilongjiang Province was analyzed in 1896, 1949 and 1981. Using total area, mean patch size, patch density, coefficient of patch size variation, mean patch fractal dimension and mean shape index, we studied the change of forest landscape pattern and the change of each patch types in this region. As a result, the total area of forest landscape and mean patch size decreased sharply, the quantity and density of patches increased, the juxtaposition of patches weakened, the shape of patch tended to become regular, and the border of patch simplified. All these showed that the forest landscape of this area tended to fragment gradually, and the fragment of Korean pine forest is the severest. The diversity of whole forest landscape and the evenness of landscape types distribution reduced gradually. Human impact, instead of climate change and forest community succession, is the most important reason for such dramatic changes.
基金Supported by National Natural Science Foundation(40971160)Project of Guizhou Provincial Natural Science Foundation(2007-2049)+2 种基金Guizhou Provincial High-grade Talents Project(TZJF-2008-40)Supporting Project for Guizhou Provincial Outstanding Young S&T Talents in 2009Key Project of Guizhou Normal University Students'Tasks~~
文摘With Maolan Nature Reserve as an example, its forest landscape characteristics and diversity were studied by utilizing GIS. The results showed that the overall forest landscape in Maolan Nature Reserve was quite good, but with the increasing natural interferences and human activities, shrub forests and non-woodland landscape types have been multiplied; among the 3 functional zones of the Nature Reserve, landscape fragmentation degree is low in core zone and buffer zone, but high in experimental zone.
基金Supported by the National Natural Science Foundation of China(31260132)National Natural Science Foundation of China(31060111)Natural Science Foundation of Inner Mongolia(2012MS0514)~~
文摘[Objective] This study aimed to investigate the fungi in Wula Mountain National Forest Park. [Method] More than 180 fungal specimens were collected from Wula Mountain National Forest Park from 2009 to 2012 for primarily studying the fungal species diversity. [Results] According to the classification system presented by Ainsworth et al. and with reference to the China catalogue of Macrofungi in species diversity catalogue of Fungi at Wula Mountain National Forest Park was written, involving in 80 species belonging to 43 genera, 22 families, 5 orders and 2 classes in the Basidiomycotina, and 6 species belonging to 2 genera, 2 families, 2 orders and2 classes in Ascomycotina, totally from 86 species, 45 genera, 7 orders, 24 families and 2 subdivisions. Among them, 49 species were edible and 22 species were medicinal, and 18 species were both edible and medicinal, and 7 species were poisonous, and 32 species were wood-rotting, and 5 species were mycorrhizal fungi,and 3 species were newly-recorded ones in Inner Mongolia. [Conclusion] There are still some specimens that have not been identified yet because of lack of literature,thus requiring further study for supplement.
