[Objective] The aim was to clear the relationship between fruit growth and development and endogenous hormones content in the pulp of Dangshansu pear.[Method] Choosing Dangshansu pear as material,the dynamic changes o...[Objective] The aim was to clear the relationship between fruit growth and development and endogenous hormones content in the pulp of Dangshansu pear.[Method] Choosing Dangshansu pear as material,the dynamic changes of endogenous hormones in pulp during various development stages were determined by means of enzyme linked immunosorbent assay（ELISA）.[Result] The results indicated that the content of endogenous hormones like ZR,GA and IAA promoting fruit growth showed increase trend within 15 d after pollination,and higher content of ZR,GA and IAA could promote ovary development and normal fruit setting of Dangshansu pear.The content of ZR in pulp had the highest value at the beginning of the first fast growth period,and then showed decrease trend until fruit became mature.The dynamic changes of IAA and GA were similar,namely their contents were higher in the first fast growth period,and decreased with fruit development,and then increased rapidly and was up to the maximum value in the second fast growth period,corresponding with the growth dynamic of fruit.The peaks of ABA content could be found on the 15th,45th and 120th day after pollination,which corresponded with the physiological fruit dropping and maturing period of Dangshansu pear.[Conclusion] The duration of various development periods and content of endogenous hormones of Dangshansu pear were different,and fruit growth and development were closely related to the content of endogenous hormones.展开更多
[Objective] To study the purification effect of plant community on wetland water environment in Hongze Lake, and to provide references to the ecological restoration of this area. [Methed] The typical lakeside zone of ...[Objective] To study the purification effect of plant community on wetland water environment in Hongze Lake, and to provide references to the ecological restoration of this area. [Methed] The typical lakeside zone of Hongze Lake Wetland National Nature Reserve was taken as the study area. Through the field survey based on environmental characteristics of aquatic areas and non-aquatic areas, combined with laboratory test, the content changes of nutrients （N and P）, COD （chemical oxygen demand） and SD （transparency） levels in the water body of lake wetland were measured; the purification effect of the lake wetland ecosystem in the growth process of aquatic vegetation was quantitatively analyzed, and possible influ- encing factors were discussed. [Result] Aquatic vegetation could remove the TN, TP, COD, Chla in wetland water environment and had obvious effects on the changes of SD content. Competition between aquatic plants can inhibit the growth of algae and relieve the eutrophication of water body. [Conclusion] This study provided theoretical basis for ecological restoration and enhancement of self-purification capability in the lakeside zone of Hongze Lake.展开更多
[Objective] The aim was to study the relationship between endogenous hormone levels and the pod lengths in Brassica napus L.[Method] Two specially-long pod lines H218 and H203,two normal pod length lines MSP334,Zhongy...[Objective] The aim was to study the relationship between endogenous hormone levels and the pod lengths in Brassica napus L.[Method] Two specially-long pod lines H218 and H203,two normal pod length lines MSP334,Zhongyou 821 and two short pod lines ＂4101＂ and Zeng 11,were exploited as experimental materials,and the endogenous IAA,iPA,GA3 and ABA content in the pods of the materials were analyzed with the method of enzyme-linked immunosorbent assay（ELISA）.[Result] IAA contents in the specially-long pod lines were lower than those in the short pod lines on the 1st and 5th day after blossom.IAA contents on the 5th and 20th day were negatively correlated to the final pod length respectively.iPA contents in the specially-long pod lines were lower than those in the short pod lines on the 5th and 20th day.iPA content on the first day was positively correlated to the final pod length,but iPA content on the fifth day,the tenth day and the fifteenth day were negatively correlated to the final pod length.GA3 content on the 1st day was positively correlated to the final pod length.ABA content on the 1st day was negatively correlated to the final pod length.[Conclusion] It was shown that iPA and IAA played important roles in the development of pod length in the B.napus lines.展开更多
[Objective] The aims were to explore the relationship between the contents of fruit mineral elements and quality features of the ＇Fuji＇ apple, screen major mineral elements of the fruit affecting fruit quality featu...[Objective] The aims were to explore the relationship between the contents of fruit mineral elements and quality features of the ＇Fuji＇ apple, screen major mineral elements of the fruit affecting fruit quality features, and set up optimum proposals of fruit mineral elements for good fruit qualities, so as to provide a theoretical basis for the reasonable orchard soil and foliar fertilizer applications to increase fruit quality and reduce the physiological diseases related to the ＇Fuji＇ apple. [Methods] The fruit mineral elements and quality indicators of ＇Fuji＂ apples were in- vestigated and analyzed, which were collected from the 153 commercial apple or- chards of ＂Fuji＇ apple located in 51 counties. The variable importance for projection （VlP） of partial least squares regression （PLS） method was used to analyze the model effect and weight analysis impact of the fruit mineral element contents to fruit quality, screen out major factors of fruit mineral elements influencing the different fruit qualities, and set up the regression equation of the fruit qualities and major fruit mineral elements. Linear programming was used to obtain optimum proposals of the fruit mineral elements to achieve good ＇Fuji＇ apple qualities. [Results] The mineral elements content and quality characteristics in ＂Fuji＇ apple fruit had great differences in the different produce regions in which the maximum content of nitro- gen, iron, zinc and boron in the ＇Fuji＇ fruit were12.06, 6.17, 7.7, and 10.08 times greater than the minimum respectively, and the differences for titratable acid and the SSC/TA ratio were 2.33 and 2.16 times respectively. The correlation analysis between the fruit mineral element contents and qualities showed that the nitrogen content of fruit had a significantly negative correlation with the soluble solid content, SSC/TA ratio and red color area, while the calcium and iron contents in the fruit were in significantly positive correlation with the soluble solid content and SSC/TA ratio. The model effect and weight analysis showed that the content of nitrogen and iron in the fruit had a greater influence on the integral fruit quality, followed by phosphorus, potassium and calcium. The variable importance for projection （VlP） technology of PLS found that the mean fruit weight was primarily affected by nitro- gen, phosphorus and potassium, and the soluble solid was primarily affected by ni- trogen, calcium and iron, while the red color area was primarily affected by nitro- gen, potassium, calcium, iron and zinc. The regression equation between fruit quality and mineral element contents showed that the mean fruit weight had a greater pos- itive effect coefficient with the content of phosphorus and potassium, and a greater negative effect coefficient with the content of nitrogen in the fruit. Moreover, the sol- uble solid had the largest negative effect coefficient with nitrogen and largest posi- tive effect coefficient with calcium and iron in the fruit. [Conclusion] The maximum content of soluble solid and titratable acid were 1.5 times more than the minimum, and nitrogen, iron, zinc and boron were 6 times more than in the ＇Fuji＇ apple fruit in the different produce regions. Therefore, it is a key technological measure to improve the overall qualities of the ＂Fuji＇ apple by decreasing the content of nitrogen, and increasing the contents of iron, phosphorus, potassium and calcium in the fruit.展开更多
The aim of the study was to investigate the immune effect of fusion protein VP4-STI. 40 mouse were randomly divided into 4 groups of test bacterin group （30 μg VP4-STI ＋0.6 μg LTB）, aluminiumhydroxide vaccine gro...The aim of the study was to investigate the immune effect of fusion protein VP4-STI. 40 mouse were randomly divided into 4 groups of test bacterin group （30 μg VP4-STI ＋0.6 μg LTB）, aluminiumhydroxide vaccine group （30 μg VP4-STI ＋Al（OH）3 gel ） , pure protein VP4- STI vaccine group （30μg VP4-STI ） and PBS control group were immunized by rhinal dripping. And then, the antibody levels of the mouse were determined. The protection effects of mouse in all immune groups were observed after the toxicity test with strong virulent strain C83902 of E. coli. Anti-VP4-STI antibodies were produced in other groups with the highest at the 6th week, except PBS control group. The antibody level in aluminiumhydroxide bactetin group was higher than that in test vaccine group. The antibody level in pure protein VP4-STI bacterin group was lower, being extremely significantly different from that in test vaccine group （ P 〈0. 001 ）. Mouse in test vaccine group and aluminium hydroxide bacterin group had better immuno-protection effect on strong virulent strain C83902 of E. coli, obviously different from that in PBS control group. The research provided basis for further improving the immunogeneticity of STI.展开更多
[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica （L.） Batsch. [Method] P. persica （L.） Batsch varieties Chongyanghong and Yanhong were used as p...[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica （L.） Batsch. [Method] P. persica （L.） Batsch varieties Chongyanghong and Yanhong were used as parents to construct F1 orthogonal group. A total of 138 FI individuals were selected as experimental materi- als for construction of color around the stone gene pool （B1） and non-color around the stone gene pool （B2） by using bulked segregant analysis （BSA） method, molec- ular markers linked to the flesh color around the stone of P. persica （L.） Batsch were selected with SSR molecular marker technology. [Result] After selection with 256 pairs of SSR primers, three pairs of molecular markers linked to the gene con- trolling flesh color around the stone of P. persica （L.） Batsch were selected （UDP96- 003, ch04g09 and UDP97-402）. In addition, genetic distances between the three molecular markers and the gene controlling flesh color around the stone of P. persi- ca （L.） Batsch were calculated, which were 16.7, 10.1 and 17.0 cM, respectively. [Conclusion] This study laid the foundation for further selection of co-dominant molecular markers with closer genetic distance.展开更多
基金Supported by Natural Science Foundation of Anhui Province "Studyon the Metaxenia Phenomenon and its Mechanism of DangshansuPear" (090411008)Anhui Agricultural Committee " Modern Agricultural Fruit Industry Technology System" (Anhui Academy of Agricultural Sciences [2011] No. 6)Anhui Provincial Finance Department " Modern Agricultural Production and Development Project oDangshansu Pear Industry of Anhui Academy of Agricultural Sciences" (Anhui Academy of Agricultural Sciences [2010] No. 53)~~
文摘[Objective] The aim was to clear the relationship between fruit growth and development and endogenous hormones content in the pulp of Dangshansu pear.[Method] Choosing Dangshansu pear as material,the dynamic changes of endogenous hormones in pulp during various development stages were determined by means of enzyme linked immunosorbent assay（ELISA）.[Result] The results indicated that the content of endogenous hormones like ZR,GA and IAA promoting fruit growth showed increase trend within 15 d after pollination,and higher content of ZR,GA and IAA could promote ovary development and normal fruit setting of Dangshansu pear.The content of ZR in pulp had the highest value at the beginning of the first fast growth period,and then showed decrease trend until fruit became mature.The dynamic changes of IAA and GA were similar,namely their contents were higher in the first fast growth period,and decreased with fruit development,and then increased rapidly and was up to the maximum value in the second fast growth period,corresponding with the growth dynamic of fruit.The peaks of ABA content could be found on the 15th,45th and 120th day after pollination,which corresponded with the physiological fruit dropping and maturing period of Dangshansu pear.[Conclusion] The duration of various development periods and content of endogenous hormones of Dangshansu pear were different,and fruit growth and development were closely related to the content of endogenous hormones.
基金Supported by the Social Development Project of Jiangsu Provincial Science and Technology Department(BK2010023)江苏省科技厅社会发展项目(BK2010023)资助
文摘[Objective] To study the purification effect of plant community on wetland water environment in Hongze Lake, and to provide references to the ecological restoration of this area. [Methed] The typical lakeside zone of Hongze Lake Wetland National Nature Reserve was taken as the study area. Through the field survey based on environmental characteristics of aquatic areas and non-aquatic areas, combined with laboratory test, the content changes of nutrients （N and P）, COD （chemical oxygen demand） and SD （transparency） levels in the water body of lake wetland were measured; the purification effect of the lake wetland ecosystem in the growth process of aquatic vegetation was quantitatively analyzed, and possible influ- encing factors were discussed. [Result] Aquatic vegetation could remove the TN, TP, COD, Chla in wetland water environment and had obvious effects on the changes of SD content. Competition between aquatic plants can inhibit the growth of algae and relieve the eutrophication of water body. [Conclusion] This study provided theoretical basis for ecological restoration and enhancement of self-purification capability in the lakeside zone of Hongze Lake.
文摘[Objective] The aim was to study the relationship between endogenous hormone levels and the pod lengths in Brassica napus L.[Method] Two specially-long pod lines H218 and H203,two normal pod length lines MSP334,Zhongyou 821 and two short pod lines ＂4101＂ and Zeng 11,were exploited as experimental materials,and the endogenous IAA,iPA,GA3 and ABA content in the pods of the materials were analyzed with the method of enzyme-linked immunosorbent assay（ELISA）.[Result] IAA contents in the specially-long pod lines were lower than those in the short pod lines on the 1st and 5th day after blossom.IAA contents on the 5th and 20th day were negatively correlated to the final pod length respectively.iPA contents in the specially-long pod lines were lower than those in the short pod lines on the 5th and 20th day.iPA content on the first day was positively correlated to the final pod length,but iPA content on the fifth day,the tenth day and the fifteenth day were negatively correlated to the final pod length.GA3 content on the 1st day was positively correlated to the final pod length.ABA content on the 1st day was negatively correlated to the final pod length.[Conclusion] It was shown that iPA and IAA played important roles in the development of pod length in the B.napus lines.
基金Supported by the Special Funds for Forestry Industry Research in Public Welfare(201404720)the National Science and Technology Support Program(2014BAD16B02-3)the Science and Technology Innovation Ability Construction Projects of Beijing Academy of Agriculture and Forestry Science(KJCX20150403)~~
文摘[Objective] The aims were to explore the relationship between the contents of fruit mineral elements and quality features of the ＇Fuji＇ apple, screen major mineral elements of the fruit affecting fruit quality features, and set up optimum proposals of fruit mineral elements for good fruit qualities, so as to provide a theoretical basis for the reasonable orchard soil and foliar fertilizer applications to increase fruit quality and reduce the physiological diseases related to the ＇Fuji＇ apple. [Methods] The fruit mineral elements and quality indicators of ＇Fuji＂ apples were in- vestigated and analyzed, which were collected from the 153 commercial apple or- chards of ＂Fuji＇ apple located in 51 counties. The variable importance for projection （VlP） of partial least squares regression （PLS） method was used to analyze the model effect and weight analysis impact of the fruit mineral element contents to fruit quality, screen out major factors of fruit mineral elements influencing the different fruit qualities, and set up the regression equation of the fruit qualities and major fruit mineral elements. Linear programming was used to obtain optimum proposals of the fruit mineral elements to achieve good ＇Fuji＇ apple qualities. [Results] The mineral elements content and quality characteristics in ＂Fuji＇ apple fruit had great differences in the different produce regions in which the maximum content of nitro- gen, iron, zinc and boron in the ＇Fuji＇ fruit were12.06, 6.17, 7.7, and 10.08 times greater than the minimum respectively, and the differences for titratable acid and the SSC/TA ratio were 2.33 and 2.16 times respectively. The correlation analysis between the fruit mineral element contents and qualities showed that the nitrogen content of fruit had a significantly negative correlation with the soluble solid content, SSC/TA ratio and red color area, while the calcium and iron contents in the fruit were in significantly positive correlation with the soluble solid content and SSC/TA ratio. The model effect and weight analysis showed that the content of nitrogen and iron in the fruit had a greater influence on the integral fruit quality, followed by phosphorus, potassium and calcium. The variable importance for projection （VlP） technology of PLS found that the mean fruit weight was primarily affected by nitro- gen, phosphorus and potassium, and the soluble solid was primarily affected by ni- trogen, calcium and iron, while the red color area was primarily affected by nitro- gen, potassium, calcium, iron and zinc. The regression equation between fruit quality and mineral element contents showed that the mean fruit weight had a greater pos- itive effect coefficient with the content of phosphorus and potassium, and a greater negative effect coefficient with the content of nitrogen in the fruit. Moreover, the sol- uble solid had the largest negative effect coefficient with nitrogen and largest posi- tive effect coefficient with calcium and iron in the fruit. [Conclusion] The maximum content of soluble solid and titratable acid were 1.5 times more than the minimum, and nitrogen, iron, zinc and boron were 6 times more than in the ＇Fuji＇ apple fruit in the different produce regions. Therefore, it is a key technological measure to improve the overall qualities of the ＂Fuji＇ apple by decreasing the content of nitrogen, and increasing the contents of iron, phosphorus, potassium and calcium in the fruit.
基金Supported by the Doctor Foundation of Xinjiang Production and Con-struction Corps(Bingbo02,NKBOISHZO8)~~
文摘The aim of the study was to investigate the immune effect of fusion protein VP4-STI. 40 mouse were randomly divided into 4 groups of test bacterin group （30 μg VP4-STI ＋0.6 μg LTB）, aluminiumhydroxide vaccine group （30 μg VP4-STI ＋Al（OH）3 gel ） , pure protein VP4- STI vaccine group （30μg VP4-STI ） and PBS control group were immunized by rhinal dripping. And then, the antibody levels of the mouse were determined. The protection effects of mouse in all immune groups were observed after the toxicity test with strong virulent strain C83902 of E. coli. Anti-VP4-STI antibodies were produced in other groups with the highest at the 6th week, except PBS control group. The antibody level in aluminiumhydroxide bactetin group was higher than that in test vaccine group. The antibody level in pure protein VP4-STI bacterin group was lower, being extremely significantly different from that in test vaccine group （ P 〈0. 001 ）. Mouse in test vaccine group and aluminium hydroxide bacterin group had better immuno-protection effect on strong virulent strain C83902 of E. coli, obviously different from that in PBS control group. The research provided basis for further improving the immunogeneticity of STI.
基金Supported by Fund of Hebei Academy of Agriculture and Forestry Sciences(A06120203)~~
文摘[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica （L.） Batsch. [Method] P. persica （L.） Batsch varieties Chongyanghong and Yanhong were used as parents to construct F1 orthogonal group. A total of 138 FI individuals were selected as experimental materi- als for construction of color around the stone gene pool （B1） and non-color around the stone gene pool （B2） by using bulked segregant analysis （BSA） method, molec- ular markers linked to the flesh color around the stone of P. persica （L.） Batsch were selected with SSR molecular marker technology. [Result] After selection with 256 pairs of SSR primers, three pairs of molecular markers linked to the gene con- trolling flesh color around the stone of P. persica （L.） Batsch were selected （UDP96- 003, ch04g09 and UDP97-402）. In addition, genetic distances between the three molecular markers and the gene controlling flesh color around the stone of P. persi- ca （L.） Batsch were calculated, which were 16.7, 10.1 and 17.0 cM, respectively. [Conclusion] This study laid the foundation for further selection of co-dominant molecular markers with closer genetic distance.
