[Objective] The aim was to explore fertilizers with different proportions of N, P and K on citrus fruit cracking and to seek best treatment in preventing fruit cracking. [Method] Compound fertilizers with different pr...[Objective] The aim was to explore fertilizers with different proportions of N, P and K on citrus fruit cracking and to seek best treatment in preventing fruit cracking. [Method] Compound fertilizers with different proportions of N, P and K were prepared, supplemented by leaf fertilizers with varying mineral elements. [Result] It is proved the treatment II containing spring fertilizers （N：P2O5：K2O=20：10：10）, summer fertilizers （N：P2O5：K2O=16：8：16）, and leaf fertilizers with calcium nitrate solu- tion （0.5%） achieved the best prevention effects of fruit cracking. In the meantime, N-to-K ratio and N-to-Ca ratio were proved of positive correlation with cracked fruits, and the higher the ratios, the higher the proportion of cracked fruits, vice versa. On other hand, Ca-to-K ratio was of negative correlation with cracked fruits, and the higher the ratio, the lower the proportion of cracked fruits. [Conclusion] In practical production, fertilization should be conducted as per soil moisture content, tree growth, and seasons, in a balanced and comprehensive way.展开更多
The dynamic concentrations of glucose, fructose, sucrose and total sugar were determined through exogenous ABA and GA3 treatments during young period of fruit and before fruit coloring in pulp of ‘Cara cara’ navel o...The dynamic concentrations of glucose, fructose, sucrose and total sugar were determined through exogenous ABA and GA3 treatments during young period of fruit and before fruit coloring in pulp of ‘Cara cara’ navel orange. The results are as fol ows： 10 mg/L ABA increased glucose, fructose and total sugar concen-tration significantly or very significantly, ABA treatment of 50 mg/L increased sucrose concentration very significantly, but ABA treatment of 100 mg/L decreased glucose concentration very significantly. GA3 treatment of lower and middle concentrations（10, 50 and 250 mg/L） increased sucrose concentration very significantly, GA3 treatment of 10 mg/L had no remarkable effect on glucose and fructose concentration but in-creased total sugar concentration very significantly, GA3 treatment of 50, 250 and 500 mg/L decreased glucose, fructose and total sugar concentration very significant-ly. Therefore, ABA treatment of lower concentration could increase one or several kinds of sugar concentration, but GA3 treatment of higher concentration （250 and 500 mg/L） restrained sugar concentration in pulp of ‘Cara cara’ navel orange seri-ously.展开更多
AIM:To investigate the growth-inhibiting and apoptosis-inducing effects of the gene MOB2 on human hepatic carcinoma cell line SMMC-7721.METHODS:The full-length cDNA of the MOB2 gene was amplified from human umbilical ...AIM:To investigate the growth-inhibiting and apoptosis-inducing effects of the gene MOB2 on human hepatic carcinoma cell line SMMC-7721.METHODS:The full-length cDNA of the MOB2 gene was amplified from human umbilical vein endothelial cells.The correct full-length MOB2 cDNA was subcloned into the eukaryotic expression vector pEGFP-C1.After lipofection of the MOB2 gene into cancer cells,the levels of MOB2 protein in the cancer cells were detected by immunoblotting.To transfect the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells,the cells were cultured in Dulbecco's Modified Eagle'sMedium with 10% fetal calf serum and glutamine,and then mixed with liposomes,Lipofectamine 2000 and the plasmid vector pEGFP-CI-MOB2.RESULTS:We observed the growth and proliferation of SMMC-7721 cells containing pEGFP-CI-MOB2 and analyzed their apoptosis and growth cycle phases by flow cytometry.We successfully transfected the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells and screened for a single clone cell containing MOB2.After transfection,MOB2 enhanced growth suppression,induced apoptosis,increased the ratio of G0/G1,significantly inhibited the advance of cell cycle phase,and arrested cells in G0/G1 phase.CONCLUSION:MOB2 overexpression induces apoptosis and inhibits the growth of human hepatic cancer cells,which may be useful in gene therapy for hepatic carcinoma.展开更多
文摘[Objective] The aim was to explore fertilizers with different proportions of N, P and K on citrus fruit cracking and to seek best treatment in preventing fruit cracking. [Method] Compound fertilizers with different proportions of N, P and K were prepared, supplemented by leaf fertilizers with varying mineral elements. [Result] It is proved the treatment II containing spring fertilizers （N：P2O5：K2O=20：10：10）, summer fertilizers （N：P2O5：K2O=16：8：16）, and leaf fertilizers with calcium nitrate solu- tion （0.5%） achieved the best prevention effects of fruit cracking. In the meantime, N-to-K ratio and N-to-Ca ratio were proved of positive correlation with cracked fruits, and the higher the ratios, the higher the proportion of cracked fruits, vice versa. On other hand, Ca-to-K ratio was of negative correlation with cracked fruits, and the higher the ratio, the lower the proportion of cracked fruits. [Conclusion] In practical production, fertilization should be conducted as per soil moisture content, tree growth, and seasons, in a balanced and comprehensive way.
基金Supported by Startup Foundation for Doctors of Yangtze University~~
文摘The dynamic concentrations of glucose, fructose, sucrose and total sugar were determined through exogenous ABA and GA3 treatments during young period of fruit and before fruit coloring in pulp of ‘Cara cara’ navel orange. The results are as fol ows： 10 mg/L ABA increased glucose, fructose and total sugar concen-tration significantly or very significantly, ABA treatment of 50 mg/L increased sucrose concentration very significantly, but ABA treatment of 100 mg/L decreased glucose concentration very significantly. GA3 treatment of lower and middle concentrations（10, 50 and 250 mg/L） increased sucrose concentration very significantly, GA3 treatment of 10 mg/L had no remarkable effect on glucose and fructose concentration but in-creased total sugar concentration very significantly, GA3 treatment of 50, 250 and 500 mg/L decreased glucose, fructose and total sugar concentration very significant-ly. Therefore, ABA treatment of lower concentration could increase one or several kinds of sugar concentration, but GA3 treatment of higher concentration （250 and 500 mg/L） restrained sugar concentration in pulp of ‘Cara cara’ navel orange seri-ously.
文摘AIM:To investigate the growth-inhibiting and apoptosis-inducing effects of the gene MOB2 on human hepatic carcinoma cell line SMMC-7721.METHODS:The full-length cDNA of the MOB2 gene was amplified from human umbilical vein endothelial cells.The correct full-length MOB2 cDNA was subcloned into the eukaryotic expression vector pEGFP-C1.After lipofection of the MOB2 gene into cancer cells,the levels of MOB2 protein in the cancer cells were detected by immunoblotting.To transfect the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells,the cells were cultured in Dulbecco's Modified Eagle'sMedium with 10% fetal calf serum and glutamine,and then mixed with liposomes,Lipofectamine 2000 and the plasmid vector pEGFP-CI-MOB2.RESULTS:We observed the growth and proliferation of SMMC-7721 cells containing pEGFP-CI-MOB2 and analyzed their apoptosis and growth cycle phases by flow cytometry.We successfully transfected the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells and screened for a single clone cell containing MOB2.After transfection,MOB2 enhanced growth suppression,induced apoptosis,increased the ratio of G0/G1,significantly inhibited the advance of cell cycle phase,and arrested cells in G0/G1 phase.CONCLUSION:MOB2 overexpression induces apoptosis and inhibits the growth of human hepatic cancer cells,which may be useful in gene therapy for hepatic carcinoma.
