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枣根际解磷细菌的分离筛选及16S rDNA鉴定 被引量:11
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作者 郭艺鹏 王海儒 +4 位作者 孙林琦 张晶晶 韩超 秦韵婷 李建贵 《河南农业大学学报》 CAS CSCD 北大核心 2015年第6期811-816,837,共7页
以新疆红枣根际土壤为材料,采用溶磷圈初筛和摇瓶复筛法筛选出土样中的解磷细菌,并对解磷细菌的16S r DNA进行测序,通过Blast检索同源序列,鉴定解磷细菌的分类。结果表明,在磷酸三钙无机磷培养基中,所筛选出的20个菌株在摇瓶培养下均有... 以新疆红枣根际土壤为材料,采用溶磷圈初筛和摇瓶复筛法筛选出土样中的解磷细菌,并对解磷细菌的16S r DNA进行测序,通过Blast检索同源序列,鉴定解磷细菌的分类。结果表明,在磷酸三钙无机磷培养基中,所筛选出的20个菌株在摇瓶培养下均有溶磷效果,其中P7,P13的能力最强,解磷量达到300 mg·L^(-1)以上,pH值在培养期间均有显著下降,菌株解磷量与培养液pH值呈极显著负相关;在蒙金娜有机磷培养基中,仅有少数几个菌株对卵磷脂有较强的解磷能力,P_3,P_7,P_9与对照间差异显著,培养液的pH值维持在一个较高水平,菌株解磷量与培养液的p H值呈极显著负相关,16S rDNA序列分析,筛选出的解磷细菌主要为Bacillus sp.,Acinetobacter sp.,Pseudomonas sp.,Enterobacter sp.等4个属的细菌。本试验最终确定的最佳解磷细菌为P_7,Bacillus sp.。 展开更多
关键词 解磷细菌 筛选 枣根际土 新疆红
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Effects of Nitrogen Fertilizer on Rhizosphere Microbial Populations under Cotton Intercropped with Red Date in Arid Desert Oasis of Southern Xinjiang
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作者 汤秋香 石大伟 +7 位作者 林涛 高文伟 田立文 郭仁松 李玉 叶强涛 林毅 徐海东 《Agricultural Science & Technology》 CAS 2014年第2期207-211,309,共6页
[Objective]This study aimed to determine the number of microorganisms of cotton rhizosphere in a soil at various amounts of applied nitrogen fertilizer in a red date-cotton intercropping system. [Method] In the field ... [Objective]This study aimed to determine the number of microorganisms of cotton rhizosphere in a soil at various amounts of applied nitrogen fertilizer in a red date-cotton intercropping system. [Method] In the field experiment, nitrogen fer-tilizer was applied at 0, 150, 300, 450, 600, and 750 kg/hm2, respectively. The dy-namic changes of the populations of bacteria, fungi and actinomycetes at the rhizo-sphere of cotton intercropped with red date were investigated. [Result] Microbial populations significantly increased at nitrogen fertilizer of 300 and 450 kg/hm2 during bud, flowering and bol opening periods. The numbers of bacteria and actinomycetes were higher during flowering and bol opening stages than at bud stage. The num-ber of fungi slightly changed during the entire growth period. Appropriate amount of nitrogen fertilizer (300 to 600 kg/hm2) was favorable to the survival of the microor-ganisms in the soil under the intercropping system. [Conclusion] The study provides a guideline for screening and determining the optimum amount of applied nitrogen fertilizer. 展开更多
关键词 Red date and cotton intercropping system Nitrogen fertilizer Rhizo-sphere soil Microbial populations
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