In order to provide reference for probiotics application in aquaculture, Bacillus subtilis （A）, Streptococcus faecalis （B） and photosynthetic bacteria （C） were prepared according to the ratios of 9：1, 4：1, 1：...In order to provide reference for probiotics application in aquaculture, Bacillus subtilis （A）, Streptococcus faecalis （B） and photosynthetic bacteria （C） were prepared according to the ratios of 9：1, 4：1, 1：1, 1：4 and 1：9 with 10^5 cfu/ml as the unit of concentration into 15 mixed microecological preparations, and their effects on COD, ammonia nitrogen, nitrite nitrogen and sulfide in pond water were investigated. The results showed that the mixed preparation of B. subtilis and photosynthetic bacteria at a ratio of 1：4 had the best effect in treating COD （P〈0.05）, the mixed preparation of B. subtilis and S. faecalis at a ratio of 4：1 showed the best effect in treating ammonia nitrogen （P〈0.05）, the mixed preparation of S. faecalis and photosynthetic bacteria at a ratio of 4：1 showed the best effect in treating nitrite nitrogen （P〈0.05）, and the mixed preparation of Streptococcus faecalis and photosynthetic bacteria at a ratio of 9：1 had the best effect of reducing sulfide （P〈0.05）.展开更多
The production and properties of the biosurfactant synthesized by Bacillus subtilis CCTCC AB93108 were studied. The maximum concentration of the surfactant is 1.64 g/L when the bacteria grow in a medium supplemented w...The production and properties of the biosurfactant synthesized by Bacillus subtilis CCTCC AB93108 were studied. The maximum concentration of the surfactant is 1.64 g/L when the bacteria grow in a medium supplemented with glucose as carbon sources. The isolated biosurfactant is a complex of protein and polysaccharide without lipids. It reduces the surface tension of distilled water to 45.9 mN/m, and its critical micelle concentration (CMC) is 2.96 g/L. It can stabilize emulsions of several aromatic and aliphatic hydrocarbons, such as benzene, xylene, n-pentane, n-nonane, gasoline and diesel oil. It presents high emulsification activity and stability in a wide range of temperature (4-100 ℃) and a long period of duration.展开更多
Bacillus subtilis strain C19, capable of ulilizing pyrene as a sole of carbon and energy, was isolated from marine Indonesian Archipelago. In biodegradation of pyrene by B. subtilis C19, various metabolites was isolat...Bacillus subtilis strain C19, capable of ulilizing pyrene as a sole of carbon and energy, was isolated from marine Indonesian Archipelago. In biodegradation of pyrene by B. subtilis C19, various metabolites was isolated and identified by chromatographic and spectral analyses. Lipopeptide biosurfactant was produced and it has stable emulsification activity. Biosurfactant was produced for enhancing pyrene uptake and bioavaibility. After 30 days incubation, there were no toxic metabolite and biodegradation of pyrene was already complete （only 0.02% undegradable metabolite）. Therefore, this strain is suitable for PAHs （polyaromatic hydrocarbons） contaminated environment recovery.展开更多
基金Supported by Science and Technology Planning Project of Hunan Province(2012NK3097)Fund for Key Discipline Construction(Zoology)of Hunan Province during the"12thFive-Year Plan"(2015-007)+1 种基金Open Fund of Hunan Province Key Laboratory of Health Aquaculture and Processing(2015-011)Science and Technology Innovation Team Plan of Hunan Provincial Colleges and Universities(2014-031)~~
文摘In order to provide reference for probiotics application in aquaculture, Bacillus subtilis （A）, Streptococcus faecalis （B） and photosynthetic bacteria （C） were prepared according to the ratios of 9：1, 4：1, 1：1, 1：4 and 1：9 with 10^5 cfu/ml as the unit of concentration into 15 mixed microecological preparations, and their effects on COD, ammonia nitrogen, nitrite nitrogen and sulfide in pond water were investigated. The results showed that the mixed preparation of B. subtilis and photosynthetic bacteria at a ratio of 1：4 had the best effect in treating COD （P〈0.05）, the mixed preparation of B. subtilis and S. faecalis at a ratio of 4：1 showed the best effect in treating ammonia nitrogen （P〈0.05）, the mixed preparation of S. faecalis and photosynthetic bacteria at a ratio of 4：1 showed the best effect in treating nitrite nitrogen （P〈0.05）, and the mixed preparation of Streptococcus faecalis and photosynthetic bacteria at a ratio of 9：1 had the best effect of reducing sulfide （P〈0.05）.
基金Project(IRT0719) supported by the Program for Changjiang Scholars and Innovative Research Team in University of China Project (2004AA649370) supported by the National High-Tech Research and Development Program of China+1 种基金 Projects(50425927, 50225926) supported by the Natural Foundation for Distinguished Young Scholars Project(2007F3102) supported by Technology Foundation for Creative Young Scholars of Fujian Province, China
文摘The production and properties of the biosurfactant synthesized by Bacillus subtilis CCTCC AB93108 were studied. The maximum concentration of the surfactant is 1.64 g/L when the bacteria grow in a medium supplemented with glucose as carbon sources. The isolated biosurfactant is a complex of protein and polysaccharide without lipids. It reduces the surface tension of distilled water to 45.9 mN/m, and its critical micelle concentration (CMC) is 2.96 g/L. It can stabilize emulsions of several aromatic and aliphatic hydrocarbons, such as benzene, xylene, n-pentane, n-nonane, gasoline and diesel oil. It presents high emulsification activity and stability in a wide range of temperature (4-100 ℃) and a long period of duration.
文摘Bacillus subtilis strain C19, capable of ulilizing pyrene as a sole of carbon and energy, was isolated from marine Indonesian Archipelago. In biodegradation of pyrene by B. subtilis C19, various metabolites was isolated and identified by chromatographic and spectral analyses. Lipopeptide biosurfactant was produced and it has stable emulsification activity. Biosurfactant was produced for enhancing pyrene uptake and bioavaibility. After 30 days incubation, there were no toxic metabolite and biodegradation of pyrene was already complete （only 0.02% undegradable metabolite）. Therefore, this strain is suitable for PAHs （polyaromatic hydrocarbons） contaminated environment recovery.
