目的以入血成分为研究对象,基于网络药理学探究衢枳壳对糖尿病起效的物质基础及作用机制。方法采用高效液相色谱串联四极杆-静电场轨道阱高分辨质谱(high-performance liquid chromatography tandem quadrupole-electrostatic field orb...目的以入血成分为研究对象,基于网络药理学探究衢枳壳对糖尿病起效的物质基础及作用机制。方法采用高效液相色谱串联四极杆-静电场轨道阱高分辨质谱(high-performance liquid chromatography tandem quadrupole-electrostatic field orbitrap high resolution mass spectrometry,HPLC-Q-Exactive Orbitrap MS/MS)对衢枳壳入血成分进行鉴定,在此基础上通过Swiss Target Prediction与SuperPred数据库预测入血成分作用靶点,同时在OMIM,GeneCards等数据库获取糖尿病靶点。采用Cytoscape 3.9.1绘制中药衢枳壳“活性成分-靶点-疾病”网络关系图,利用String数据分析平台进行蛋白互作(protein-protein interaction,PPI)网络分析,筛选关键靶点。通过DAVID数据库对关键靶点进行基因本体功能(gene ontology,GO)和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。应用Autodock 1.5.7软件进行分子对接验证。结果共鉴定衢枳壳入血成分20个,筛选出潜在靶点170个,核心靶点32个。GO功能富集和KEGG信号通路分析结果显示缺氧诱导因子(hypoxia-inducible factor,HIF)-1信号通路、晚期糖基化终末产物(advanced glycation end products,AGE)-晚期糖基化终产物受体(receptor for advanced glycation end products,RAGE)信号通路、表皮生长因子(epidermal growth factor receptor,EGFR)信号通路、癌症蛋白聚糖通路等为衢枳壳降糖的关键通路,丝氨酸/苏氨酸蛋白激酶(RAC serine/threonine-protein kinase,AKT)1、白蛋白(albumin,ALB)、细胞肿瘤抗原p53(cellular tumor antigenp 53,TP53)、肿瘤坏死因子(tumor necrosis factor,TNF)、EGFR为其中关键靶点,且衢枳壳中5个活性成分与核心靶点经分子对接后的结合活性较好。结论衢枳壳中的芦丁、新橙皮苷、橙皮苷、芸香柚皮苷、川陈皮素等可能为衢枳壳降糖的物质基础,可能是通过调控HIF-1、AGE-RAGE、EGFR等信号通路及AKT1、ALB、TP53等核心基因发挥降糖作用。展开更多
Fruits and vegetables are an essential part of a healthy diet, providing humans with vitamins, phytonutrients, and minerals. They are significantly vulnerable, however, to post-harvest diseases caused by numerous fung...Fruits and vegetables are an essential part of a healthy diet, providing humans with vitamins, phytonutrients, and minerals. They are significantly vulnerable, however, to post-harvest diseases caused by numerous fungal and bacterial pathogens. These pathogens can cause significant quantitative and qualitative losses from harvest to consumption during the handling and storage processes. Chemical fungicides are commonly used but are likely to leave residues on the produce, rendering short shelf-life produce, such as berries, unsuitable for human consumption. Identifying eco-friendly methods to control post-harvest disease is, therefore, of utmost importance. The presence of antifungal constituents in the roots of Poncirus trifoliata extracts was detected by thin layer chromatography-based bioautography. The active constituents were isolated and identified by bioautography assay-guided fractionation using flash chromatography followed by spectroscopic techniques. In this study, xanthoxyletin, demethylsuberosin, dentatin, nordentatin, ponfolin, and clausarin were isolated from the root extracts. The antifungal activity of these compounds was moderate to weak compared to the commercial fungicide captan. This study reports the isolation and identification of natural compounds from Poncirus trifoliata that exhibited antifungal activity against Colletotrichum fragariae and Botrytis cinerea, two major post-harvest pathogens.展开更多
提出了题示方法测定衢枳壳样品中黄曲霉毒素B1、B2、G1、G2和赭曲霉毒素A的含量。样品经过体积比79∶20∶1的乙腈-水-乙酸混合溶液超声提取,离心,上清液用p H 7.0磷酸盐缓冲液稀释,用复合免疫亲和柱净化,过滤,滤液中的目标物在XDB C18...提出了题示方法测定衢枳壳样品中黄曲霉毒素B1、B2、G1、G2和赭曲霉毒素A的含量。样品经过体积比79∶20∶1的乙腈-水-乙酸混合溶液超声提取,离心,上清液用p H 7.0磷酸盐缓冲液稀释,用复合免疫亲和柱净化,过滤,滤液中的目标物在XDB C18色谱柱上用不同体积比的体积比1∶9的甲醇-5 mmol·L^(-1)乙酸铵混合溶液和体积比9∶1的甲醇-5 mmol·L^(-1)乙酸铵混合溶液的混合溶液分离,电喷雾离子源正离子(ESI+)和多反应监测(MRM)模式检测,同位素内标法定量。结果显示:5种真菌毒素与其同位素内标质量浓度比和对应的峰面积比在一定范围内呈线性关系,检出限为0.05~0.20μg·kg^(-1)。3个加标浓度水平下阴性样品中5种真菌毒素的回收率为82.7%~97.3%,测定值的相对标准偏差(n=6)为5.2%~12%。展开更多
文摘目的以入血成分为研究对象,基于网络药理学探究衢枳壳对糖尿病起效的物质基础及作用机制。方法采用高效液相色谱串联四极杆-静电场轨道阱高分辨质谱(high-performance liquid chromatography tandem quadrupole-electrostatic field orbitrap high resolution mass spectrometry,HPLC-Q-Exactive Orbitrap MS/MS)对衢枳壳入血成分进行鉴定,在此基础上通过Swiss Target Prediction与SuperPred数据库预测入血成分作用靶点,同时在OMIM,GeneCards等数据库获取糖尿病靶点。采用Cytoscape 3.9.1绘制中药衢枳壳“活性成分-靶点-疾病”网络关系图,利用String数据分析平台进行蛋白互作(protein-protein interaction,PPI)网络分析,筛选关键靶点。通过DAVID数据库对关键靶点进行基因本体功能(gene ontology,GO)和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。应用Autodock 1.5.7软件进行分子对接验证。结果共鉴定衢枳壳入血成分20个,筛选出潜在靶点170个,核心靶点32个。GO功能富集和KEGG信号通路分析结果显示缺氧诱导因子(hypoxia-inducible factor,HIF)-1信号通路、晚期糖基化终末产物(advanced glycation end products,AGE)-晚期糖基化终产物受体(receptor for advanced glycation end products,RAGE)信号通路、表皮生长因子(epidermal growth factor receptor,EGFR)信号通路、癌症蛋白聚糖通路等为衢枳壳降糖的关键通路,丝氨酸/苏氨酸蛋白激酶(RAC serine/threonine-protein kinase,AKT)1、白蛋白(albumin,ALB)、细胞肿瘤抗原p53(cellular tumor antigenp 53,TP53)、肿瘤坏死因子(tumor necrosis factor,TNF)、EGFR为其中关键靶点,且衢枳壳中5个活性成分与核心靶点经分子对接后的结合活性较好。结论衢枳壳中的芦丁、新橙皮苷、橙皮苷、芸香柚皮苷、川陈皮素等可能为衢枳壳降糖的物质基础,可能是通过调控HIF-1、AGE-RAGE、EGFR等信号通路及AKT1、ALB、TP53等核心基因发挥降糖作用。
文摘Fruits and vegetables are an essential part of a healthy diet, providing humans with vitamins, phytonutrients, and minerals. They are significantly vulnerable, however, to post-harvest diseases caused by numerous fungal and bacterial pathogens. These pathogens can cause significant quantitative and qualitative losses from harvest to consumption during the handling and storage processes. Chemical fungicides are commonly used but are likely to leave residues on the produce, rendering short shelf-life produce, such as berries, unsuitable for human consumption. Identifying eco-friendly methods to control post-harvest disease is, therefore, of utmost importance. The presence of antifungal constituents in the roots of Poncirus trifoliata extracts was detected by thin layer chromatography-based bioautography. The active constituents were isolated and identified by bioautography assay-guided fractionation using flash chromatography followed by spectroscopic techniques. In this study, xanthoxyletin, demethylsuberosin, dentatin, nordentatin, ponfolin, and clausarin were isolated from the root extracts. The antifungal activity of these compounds was moderate to weak compared to the commercial fungicide captan. This study reports the isolation and identification of natural compounds from Poncirus trifoliata that exhibited antifungal activity against Colletotrichum fragariae and Botrytis cinerea, two major post-harvest pathogens.
