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柏木叶中多醣提取技术的研究
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作者 毛燕 《浙江林业科技》 北大核心 2000年第3期51-53,共3页
用正交试验结果作方差分析表明 :提取时间、提取次数、提取温度及溶剂体积对柏木叶多醣的提取均有显著影响 ,醇析作用分离未见多醣的得率与乙醇体积分数相关 ,当乙醇体积为 60 %~ 70 %时 ,粗品多醣的含量达到最大值 4 .0 8%
关键词 柏木叶 多醣 提取工艺 药用价值
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不同化学型柏木叶精油抗氧、抑菌及对秀丽隐杆线虫的活性试验 被引量:3
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作者 陈华铮 王侨 朱凯 《林业工程学报》 CSCD 北大核心 2022年第4期72-77,共6页
为了解决化学药品滥用而带来的病虫害抗性问题,笔者以6种不同化学型的墨西哥柏木叶精油为试验对象,采用清除2,2′-叠氮基双(3-乙基苯并噻唑-6-磺酸)二铵盐(ABTS^(+))和2,2-二苯基-1-吡啶并肼基(DPPH)自由基的方法,探讨其体外抗氧化能力... 为了解决化学药品滥用而带来的病虫害抗性问题,笔者以6种不同化学型的墨西哥柏木叶精油为试验对象,采用清除2,2′-叠氮基双(3-乙基苯并噻唑-6-磺酸)二铵盐(ABTS^(+))和2,2-二苯基-1-吡啶并肼基(DPPH)自由基的方法,探讨其体外抗氧化能力;通过抑菌圈大小和最小抑菌杀菌浓度来判断墨西哥柏木叶精油的杀菌抑菌活性;通过对秀丽隐杆线虫的毒性来评估其杀虫驱虫活性。结果表明,香紫苏型对ABTS^(+)自由基清除效果最佳,其清除率达10%时所用精油的质量浓度(LC_(10))为1.0134 mg/mL,依兰油型对DPPH自由基清除效果最佳(LC_(10)为0.8931 mg/mL)。香紫苏型和依兰油型对枯草芽孢杆菌的抑制效果最好,当体积分数为100%时,抑菌圈直径约为11 mm,最小抑菌浓度和最小杀菌浓度分别为3.75和7.50 mg/mL。高浓度时香紫苏型对线虫毒杀效果最佳,且能很好地破坏线虫体内的氧化平衡,加速线虫死亡;100 mg/L质量浓度下,柠檬烯型可抑制过氧化氢酶活性,香紫苏型可抑制谷胱甘肽酶活性,蒎烯型可抑制超氧化物歧化酶活性。研究结果表明,墨西哥柏木叶精油对秀丽隐杆线虫体内抗氧化酶活性有很大影响,能缩短秀丽隐杆线虫的寿命。在开发环境友好型杀菌剂和杀虫剂时,可以使用墨西哥柏木叶精油作为增效剂,以提高其综合杀灭能力。 展开更多
关键词 墨西哥柏木叶精油 体外抗氧 杀菌抑菌 秀丽隐杆线虫 毒杀效果
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Variable chlorophyll fluorescence in response to water plus heat stress treatments in three coniferous tree seedlings 被引量:10
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作者 喻方圆 Robert D.Guy 《Journal of Forestry Research》 SCIE CAS CSCD 2004年第1期24-28,J001-J002,共7页
Effects of water and heat stress treatments on chlorophyll fluorescence of Chinese fir (Cunninghamia lanceolata), Masson pine (Pinus massoniana) and western redcedar (Thuja plicata D. Don)_seedlings were monitored dur... Effects of water and heat stress treatments on chlorophyll fluorescence of Chinese fir (Cunninghamia lanceolata), Masson pine (Pinus massoniana) and western redcedar (Thuja plicata D. Don)_seedlings were monitored during a three-cycle stress period. It was shown that ratio of variable to maximal chlorophyll fluorescence (Fv/Fm) of these three species responded differently to water stress treatments. The Fv/Fm ratio of western redcedar decreased dramatically after water stress, while that of Chinese fir had only a slight reduction and that of Masson pine had no significant change. The experiment also showed that the Fv/Fm ratio of all three species differed significantly under heat stress treatments. Concerning three different water plus heat stress cycles, it was found that the Fv/Fm ratios of Chinese fir and Masson pine measured at the end of each water plus heat stress cycle were not significantly different. However, the Fv/Fm ratio of western redcedar was diminished significantly in response to an increase of stress time. Keywords Chinese fir - Chlorophyll fluorescence - Heat stress - Masson pine - Water stress - Western redcedar CLC number Q945.17 - S791.248 Document code A Biography: Yu Fang-yuan (1965-), male, Ph. Doctor. Associate professor in College of Forest Resources and Environment, Nanjing, Forestry University, Nanjing 210037, P. R. China.Responsible editor: Zhu Hong 展开更多
关键词 Chinese fir Chlorophyll fluorescence Heat stress Masson pine Water stress Western redcedar
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