Influence of Rainfall Run-off in Hydrologic Process on Non-Point Pollution

[Objective] This study aimed to study on influence of rainfall runoff on non-point pollution and to reduce the pollution through control of the contamination produced from rainfall runoff. [Method] In order to explore effective methods to decrease non-point pollution, we conducted analysis on hydrological process of rainfall runoff, interaction mechanism between the process and non-point pollutants, the influence on non-point pollution and hydrological model application in the research. [Result] It was proved that rainfall runoff was the main factor of non-point pollution. Control from source strengthened clearing and controlling of non-point pollutants on the ground. Growing plants in slope effectively reduced the scour and erosion of rainfall runoff on soil. The study became simple thanks for the hydrological process. [Conclusion] The research indicated that non-point pollution would be effectively reduced through control of rainfall runoff.

Chromosome Preparation and Preliminary Observation of Two Amphioxus Species in Xiamen

Amphioxus has an important evolutionary position as a result of their phylogenetic position relative to vertebrates. Understanding their chromosomes would provide key points in the study of evolutionary biology and comparative genomics. The difficulty in preparing amphioxus chromosomes currently provides a significant hurdle in this research. In the current study, we describe an improved method for metaphase preparation from amphioxus embryos and methodology for preparing metaphase spreads from regenerative somatic cells. Chromosomes of two amphioxus species from Xiamen waters in China are also observed. The diploid chromosome number was found to be 40 in Branchiostoma belcheri, while B. japonicum has 36, confirming the two are distinct species from cytotaxonomic viewpoint.

Repeat Sequences and Base Correlations in Human Y Chromosome Palindromes

On the basis of information theory and statistical methods, we use mutual information, n- tuple entropy and conditional entropy, combined with biological characteristics, to analyze the long range correlation and short range correlation in human Y chromosome palindromes. The magnitude distribution of the long range correlation which can be reflected by the mutual information is PS〉PSa〉PSb （P5a and P5b are the sequences that replace solely Alu repeats and all interspersed repeats with random uneorrelated sequences in human Y chromosome palindrome 5, respectively）; and the magnitude distribution of the short range correlation which can be reflected by the n-tuple entropy and the conditional entropy is PS〉P5a〉PSb〉random uncorrelated sequence. In other words, when the Alu repeats and all interspersed repeats replace with random uneorrelated sequence, the long range and short range correlation decrease gradually. However, the random nncorrelated sequence has no correlation. This research indicates that more repeat sequences result in stronger correlation between bases in human Y chromosome. The analyses may be helpful to understand the special structures of human Y chromosome palindromes profoundly.

Construction of a full bacterial artificial chromosome(BAC) library of Oryza sativa genome

We have constructed a full BAC library for the superior eaxly indica variety of OryZa sativa, Guang Lu Ai4. The MAX Efficiency DHlOB with increased stabilltyof inserts was used as BAC host cells. The potent pBelo BACII with double selection markers was used as cloning vector. The cloning efficiency we have reached was as high as 98%, and the transformation efficiency was raised up to 1Oo transformants / pg of large fragment DNA. The BAC recombinant transformants were picked at random and analyzed for the size of inserts, which turned out to be of 120 kb in length on average. We have obtained more than 20,000 such BAC clones. According to conventional probabillty equation, they covered the entire rice genome of 420,000 kb in length. The entire length of inserts of the library obtained has the 5- to 6- fold coverage of the genome. To our knowledge, this is the first reported full BAC library for a complex genome.

Antimicrobial management of intra-abdominal infections:Literature's guidelines

Antimicrobial management of severe intra-abdominal infections (IAIs) involves a delicate balance of optimizing empirical therapy,which has been shown to improve clinical outcomes,while simultaneously reducing unnecessary antimicrobial use.Two sets of guidelines for the management of intra-abdominal infections were recently published.In 2010,the Surgical Infection Society and the Infectious Diseases Society of America (SIS-IDSA) created guidelines for the diagnosis and management of complicated IAIs.The new SIS-IDSA guidelines replace those previously published in 2002 and 2003.The World Society of Emergency Surgery (WSES) guidelines represent additional contributions,made by specialists worldwide,to the debate regarding proper antimicrobial drug methodology.These guidelines represent the conclusions of the consensus conference held in Bologna,Italy,in July 2010 during the first congress of the WSES.

Construction of a DNA library from chromosome 4 of rice(Oryza sativa)by microdissection

A simple method to create a chromosome-specific DNA library of rice, including microdissection, amplification,characterization and cloning, is described. Rice chromosome 4 from a metaphase cell has been isolated and amplified by the Linker Adapter PCR (LA-PCR). The PCR products were labeled as probes with DIG-11-dUTP using the random priming method.Southern blot analysis with rice genomic DNA and specific RFLP markers demonstrated that the PCR products were derived from rice chromosome 4. A large library comprising over 100,000recombinant plasmid microclones from rice chromosome 4was constructed. Colony hybridization showed that 58% of the clones contained single or low-copy sequences and 42%contained repetitive sequences. The size of inserts generated by PCR ranged from 140bp to 500bp.This method will facilitate cloning of the specific chromosome DNA markers and important genes of rice.

Tracing Changes in the Microbial Community of a Hydrocarbon-Polluted Soil by Culture-Dependent Proteomics

Hydrocarbon contamination may affect the soil microbial community, in terms of both diversity and function. A laboratory experiment was set-up, with a semi-arid control soil and the same soil but artificially contaminated with diesel oil, to follow changes in the dominant species of the microbial community in the hydrocarbon-polluted soil via proteomics. Analysis of the proteins extracted from enriched cultures growing in Luria-Bertani （LB） media showed a change in the microbial community. The majority of the proteins were related to gIycolysis pathways, structural or protein synthesis. The results showed a relative increase in the complexity of the soil microbial community with hydrocarbon contamination, especially after 15 days of incubation. Species such as Ralstonia solanacearum, Synechococcus elongatus and different Clostridium sp. were adapted to contamination, not appearing in the control soil, although Bacillus sp. dominated the growing in LB in any of the treatments. We conclude that the identification of microbial species in soil extracts by culture-dependent proteomics is able to partially explain the changes in the diversity of the soil microbial community in hydrocarbon polluted semi-arid soils, but this information is much more limited than that provided by molecular methods.

Study on the Promotion Strategy of College Students’ Reading and Writing Ability Under the Micro Network Environment

Network media has become more and more involved in our daily life. It has become a part of modern life that cannot be stopped or rejected. Then, how to correctly face the changes in the Internet era is placed in front of everyone. Students need to “learn how to use the media to get their all-round development, effectively prevent the foreign information pollution, and actively guide students to a truly rational attitude for WeChat, blog and micro-blog”. It is a kind of media literacy being confronted with the network’s ability to consciously resist the harmful information, but also to benefit their own development and firmly grasp the information, so as to make full use of the convenience of network self promotion. “Screen reading” network plays an important role in college students’ life, and the fragmentation, the text features of the writing ability of college students also have a strong impact; this paper focuses on the influence of college students in the network reading and writing patterns on their writing ability and influence. From the perspective of media literacy education for college students, writing ability and reading ability provide a feasible way.

Bibliometric analysis of research on soil arsenic during 2005–2016

Bibliometrics is an important branch in the field of information science,and it is widely used in many disciplines in modern research.A current active research subject can be analyzed and summarized from many aspects,such as the core institutions,core authors,highly cited papers,and keywords by using bibliometrics.This paper describes a bibliometric analysis regarding soil arsenic content by using the database from Web of Science to compare relevant research from work done domestically and abroad from 2005 to 2016.The results show that the relevant publications in China and overseas both dramatically increased from 2009,which indicates that research activity is expanding.The USA produced 27.3%of all relevant articles followed by China with 26.9%and India with 9.5%.Key issues mainly focused on agriculture and environmental science.Major journals studied include the following:Science of the Total Environment,Environmental and Experimental Botany,Environmental Pollution,Chemosphere,Journal of Hazardous Materials,Journal of Environmental Chemical Engineering,Journal of Geochemical Exploration,Ecotoxicology and Environmental Safety,Environmental Research,Talanta,and Applied Geochemistry.These are the most important journals in this field.In light of cooperation between countries,it is clear that China and the United States occupy the leading position.

Immunoscintigraphy with ^99mTc-Besilesomab in Aid to Fusiariosis Detection in Patients with Relapsed Acute Myeloid Leukemia （AML）： A Case Report and Literature Review

The aim of this article is to emphasize the importance of nuclear medicine in fungal infection characterization through a case report. Case： patient female, with AML-M2, hospitalized with fever, dyspnea, nausea, myasthenia, abdominal pain and diarrhea. In the physical examination exacerbated lesion in abdominal wall and subcutaneous nodules in upper and lower limbs. The computed tomography of the chest evinced area of attenuation in ground-glass opacities in the middle lobe, tending to consolidation and bilateral nodular opacities with ground-glass halo which suggested an infiammatory/infectious process of fungal etiology. Immunoscintigraphy with ^99mTc-besilesomab （Scintimun） identified focal infectious processes in activity, scattered over cutaneous and subcutaneous tissues, predominantly in extremities. In blood culture test, there was a growth ofFusarium sp. There is a difficulty in the diagnosis of fungal infections, both in clinical and imaging methods. There is no specific method to reaching the target. In addition, fungal diseases can be divided into focally localized or disseminated infections. There is a greater difficulty in the diagnosis of immunocompromised individuals due to the deficiency of basic defense mechanisms. Scintimun （scintigraphy with besilesomab） is a useful tool of nuclear medicine in the diagnosis of infectious and inflammatory diseases to allow the image of the whole-body and in vivo detection of early pathological and physiological phenomena, even before anatomical alterations occur.

The Effect of the Hot Springs at the Umm Qeis Archaeological Site and Museum

Several recent studies have pointed out that northern parts of Jordan are exposed to high levels of the effect of the hot spring, which is widely known to have severe impacts on health, vegetation and structures. North Jordan has a wealth of ancient heritage including six out of the ten Decapolis Cities. Air pollution that comes out from the hot springs is alleged to deteriorate and erode buildings, structures, statues and monuments through acid deposition. Therefore, this research was conducted to assess air quality at Gadara, the capital of the ancient Decapolis and suggest mitigation measures that have to be adopted in order to save the ancient heritage against further deterioration. Measurements revealed that most criteria air pollutants including sulfur dioxide, nitrogen dioxide and carbon monoxide attain corresponding national ambient air quality standards. Atmospheric concentrations of NO are strongly linked to the amount of 03. Simultaneous exposure to SO2 and NO2 demonstrated that a catalytic reaction takes place which increases the rate of sulphate production after 30 h exposure.

Influence of Air Pollution on the Deterioration of Monuments and Museum Collections

The time has come to recognize the extreme importance of the archaeological sites in Jordan. They provide a wealth evidence and precise information regarding ancient civilizations and cultures. The safeguarding of this cultural heritage is one of the most urgent priorities, that involve the proper conservation and preservation methods of all artifacts and buildings that have an important artistic and historic value. Conservation is a whole field in regarding the care and treatment of valuable artifacts, both movable and immovable. It has two aspects： （1） the control of the environment--to minimize the decay of artifacts and materials and （2） the treatment of these--in order to stop or slow down any deterioration and to stabilize them where possible against further dilapidation. The museum environment is a limited space dedicated not only to exhibition but also to the appropriate conservation of works of art. This article presents an overview of the types of damage and deterioration that air pollution causes to indoor cultural heritage materials and monuments. It identifies the main damaging air pollutants from outdoor sources to be sulphur dioxide, nitrogen dioxide, ozone and sulphide gases.

Creating a Geographical Observatory of Atmospheric Pollution （GEOAP） for the Greek Territory

The main purpose of this paper is to analyze the necessity of a Geographical Observatory of Atmospheric Pollution （GEOAP） in the Greek territory. The analysis performed is mainly focused on the benefits of the future function of the GEOAP to the environmental planning of the country and it could also provide an environmental management tool for the whole region. Measuring and mapping the pollution data and at the same time performing the geographical analysis of the complexity and the characteristics of natural and human environment can be useful tool in observation, management, and planning of the environmental policy of the country.

Effect of Polyvinylpyrrolidone on the Morphologies of Latex Films and Air Permeability of the Latex Finished Fabrics

Low air permeability is a common problem happening to the coating finished and pigment dyed fabrics.To tackle the problem,styrene and butyl acrylate copolymer latex was prepared and used as substitute for the binder.Then,polyvinylpyrrolidone(PVP)was fed into the latex.It was expected that pores could form in the resulting latex film after being rinsed.The morphologies of the latex films were analysized by scanning electric microscope(SEM).It was found that the continuity of the latex films decreased greatly because of the addition of PVP,leading to the loss of the stress at break of the films.After rinsing with methanol aqueous solution,PVP was easily leached off and(macro)void was formed in the latex films.The size of the void decreased with the increase of the PVP concentration.Then,the latex with and without PVP was used to finish the cotton fabrics and engaged as binder in pigment dyeing.After PVP was rinsed out,the air permeability of the fabrics improved greatly,which recovered to that of the unfinished one.In the meantime,the color fastness to rubbing was little affected.So,the method of PVP addition could be termed as an efficient way to improve the air permeability in coating and dyeing industry.

Blue Shallow Wounds Of Pad Dyeing ——Exploration And Reflection On Dali Tourism

Dali is an ancient city on the southwestern border of China＇ s territory, she sprouted seeds of human civilization, experienced the vicissitudes of the millennium,eventually formed a rich ethnic customs.She is ＂the cultural crossroads of the ancient capital of Asia＂ which is recognized by the world.However, the beauty of the past has been almost no advantage in today＇ s tourism industry accounts for Dianxi rapid development.Many traditional attractions false propaganda. Those views may already be the last generation of human memory even documented. Dali almost forgotten their own advantage in such under the impact,for the construction and development of cultural tourism in Dali, but also for thousands of years to live up to this cultural accumulation, this paper analyzes the development direction of the national Culture Tourism in Dali Dali from the perspective of the transformation.

Preparation of Latex Film with Macropores and Their Applications in Pigment Dyeing

Aiming at improving the permeability of the pigment dyed fabrics,two kinds of hydrophilic polymers(polyvinyl pyrrolidone(PVP),and polyethylene glycol(PEG)were fed into the styrene-butyl acrylate(St-BuA)copolymer latex binder respectively to prepare films with macropores.The effects of the post-added polymers on the latex film formation process and film structures were studied and the performance of the dyed fabrics was evaluated.It was found that the drying process could still be divided into three stages even after the addition of PVP and PEG.And the water evaporation rate during the first and last stage remained the same as usual.However,after the addition of PVP,the onset of the second stage was delayed to high volume fraction,and PVP formed into spherical dispersion phase with 300 nm in diameter.It provided a great deal of interface between the latex polymer and the PVP phase,which led to an increase in the water evaporation rate during the second stage.A different case was found after the feeding of PEG.Firstly,the first stage ended at low volume fraction and a decreased evaporation rate was observed in the second stage.Secondly,the PEG dispersion appeared as finger-like structure in the transmission electron microscopy(TEM)images with 9μm in length.After rinsing,pores were found only in the films formerly containing PVP or PEG,and the shapes and the sizes were closely correlated with the initial morphologies of the PVP or PEG domains.However,the shade of color,the abrasion fastness,and the permeability of the dyed fabric were independent of the type of the post-added hydrophilic polymer.

Construction of cDNA Library from Intestine, Mesentery and Coelomocyte of Apostichopus japonicus Selenka Infected with Vibrio sp. and a Preliminary Analysis of Immunity-Related Genes

The aquaculture of sea cucumber Apostichopus japonicus (Echinodermata, Holothuroidea) has grown rapidly during recent years and has become an important sector of the marine industry in Northern China. However, with the rapid growth of the industry and the use of non-standard culture techniques, epidemic diseases of A. japonicus now pose increasing problems to the industry. To screen the genes with stress response to bacterial infection in sea cucumber at a genome wide level, we constructed a cDNA library from A. japonicus Selenka (Aspidochirotida: Stichopodidae) after infecting them with Vibrio sp. for 48 h. Total RNA was extracted from the intestine, mesentery and coelomocyte of infected sea cucumber using Trizol and mRNA was isolated by Oligotex mRNA Kits. The ligated cDNAs were transformed into DH5α, and a library of 3.24×105 clones (3.24×105 cfu mL-1) was obtained with the sizes of inserted fragments ranging from 0.8 to 2.5 kb. Sequencing the cDNA clones resulted in a total of 1106 ESTs that passed the quality control. BlastX and BlastN searches have identified 168 (31.5%) ESTs sharing significant homology with known sequences in NCBI protein or nucleotide databases. Among a panel of 25 putative immunity-related genes, serum lectin isoform, complement component 3, complement component 3-like genes were further studied by real-time PCR and they all increased more than 5 fold in response to Vibrio sp. challenge. Our library provides a valuable molecular tool for future study of invertebrate immunity against bacterial infection and our gene expression data indicates the importance of the immune system in the evolution and development of sea cucumber.

Effect of Chloride on the Atmospheric Corrosion of Cast Iron in Sulphur or Nitrogen-Bearing Pollutant Environment

The effect of chloride on the atmospheric corrosion of cast iron in sulphur or nitrogen-bearing pollutant was investigated by using periodic wet-dry test, electrochemical experiment and surface tension test. Scanning electron microscopy coupled with energy dispersive atomic （EDAX） and stereoscopic microscopy was used to identify the corrosion processes and products. Cl^- and NO3^- were shown accelerating effects during the whole corrosion process but depression effects were observed in Cl^- and HSO3^- bearing pollutant at the initial corrosion stage. However, with the corrosion going on, the depression effects was less obviously and the initial corrosion process was investigated from the viewpoint of surface activity. At the initial corrosion stage, the corrosion rate was proportional to the adsorptivity of anions, but as corrosion went on, the penetration effect of anions and different characteristics of the corrosion products began to dominate the corrosion process, which led to changes on the corrosion rate.

Marie Corelli＇s The Secret Power in Bengali Rendering： Translation, Indianisation and Cultural Criss-crossing

Since the 1980s and 1980s, a new trend in the discipline of Translation Studies has emerged beyond the boundaries of Europe. Scholars from post-colonial nations like Canada, India, China, Africa, and Latin America have argued that translation was used in the past as a means of depriving the colonised people of their voice. In the colonial structure, the hegemonic culture used to dominate, making the others subservient. Translation in the colonial period reflected that hierarchy. In this light, decades after the independence of India, translation from a reverse direction, that is, rendering popular English texts in minority languages, may be seen as a process of＂writing back＂. Even if the original text concerned is not a ＂political＂ writing, and the translation is apparently meant for ＂safer-zones＂ such as ＂young students＇ literature＂, a tendency of claiming cultural equality, overlapping the translator＇s own cultural and literary heritage with the original author＇s, can be discovered. This paper attempts to focus on these issues in a particular text—the 1995 Bengali translation of Marie Corelli＇s 1921 science-fiction, The Secret Power. The purpose of this paper is to explore how Sudhindranath Raha has intermingled Biblical references and Western contexts with allusions to Indian culture and Sanskrit literature, thereby claiming a space for mutual understanding and respect between two cultures.

Primary malignant melanoma of the liver：One case report and literature review

Objective Primary malignant melanomas of the liver are exceedingly rare.Only 19 cases have been reported in the literature worldwide.In this report,we describe our pathological findings and review the literature in order to improve our understanding of the disease and prevent misdiagnosis,as well as provide evidence for its treatment and prognosis.Methods We present a case of an isolated malignant melanoma of the liver in a 61-year-old male Chinese patient.Results Comprehensive dermatological and ophthalmological examinations did not reveal any evidence of a primary cutaneous or ocular lesion.Similarly,serial physical examinations,auxiliary examinations,and bone scans did not demonstrate any other lesions in the brain,respiratory tract,and gastrointestinal tract.Microscopic examination of the resected specimen revealed malignant melanoma,which was confirmed by immunohistochemical staining for S-100 protein(+),ki67(30%+),EMA(+),CD10(+),and HMB-45(++).Conclusion Primary malignant melanoma may occur in the liver,and should be considered when the histopathological appearance is atypical of other hepatic neoplasms.The diagnostic criteria for hepatic malignant melanoma depend mainly on the clinical,radiographic,and histopathological findings.Pathomorphology and immumohistochemical staining can be utilized to confirm the diagnosis.