复合激活剂对混合菌群降解活性艳蓝的促进作用及生物降解机理

为探究模拟茶叶渣中关键成分制成的复合激活剂,提高两种不同混合菌群DDMY1、DDMY2对活性艳蓝19(reactive brilliant blue 19,RB19)脱色降解的机理,通过液相色谱质谱联用仪、高通量测序、非标及定量蛋白质组学进行分析。试验结果表明,经过72 h的培养,复合激活剂使菌群DDMY1的脱色率提高了7.24%,而菌群DDMY2的脱色率仅提高了1.77%,其对菌群DDMY1的促进作用明显高于对菌群DDMY2的作用。同时,激活剂能够富集肠杆菌属,这可能是增强混合菌群对RB19脱色降解的原因。在激活剂诱导下两种不同混合菌群DDMY1、DDMY2差异蛋白参与的KEGG(Kyoto Encyclopedia of Genes and Genomes)通路主要为代谢途径、次生代谢产物的生物合成、抗生素的生物合成、碳代谢。除上述的通路外,苯甲酸的降解途径也得到了证实。推测RB19被先降解为苯甲酸酯,随后进行糖酵解、柠檬酸循环等。研究结果对于激活剂强化染料生物降解机理研究具有一定指导意义。

厌氧/好氧膜生物反应器对不同pH模拟印染废水的脱色过程分析

采用厌氧/好氧膜生物反应器(A/O MBR),以艳蓝KN-R活性染料为模拟染料,在不同进水pH条件下,系统考察了A/O MBR各反应段出水的紫外-可见光谱谱图特征,初步探索pH变化时厌氧与好氧污泥对染料的降解情况。结果表明,在中性和偏酸性的进水条件下吸收带的强度较强,在酸性和偏碱性的进水条件下较弱,并且紫外和可见吸收带的峰强呈正相关关系。同时,吸收带强度减弱程度直接影响染料的脱色效果,二者呈正相关关系。碱性进水有助于提高厌氧脱色效果,酸性进水有利于提高好氧脱色效果。

Decolorization of Anthraquinone dye by Rhodopseudomonas XL-1

Rhodopseudomonas XL 1 gained from textile wastewater can effectively decolorize anthraquinone dye. Under anaerobic condition, 93 percent of the anthraquinone dye is decolorized , which is higher than that under aerobic condition. The optimum pH is 6～9 and the optimum temperature is 20～40℃ for the anthraquinone dye decolorization by XL 1 . XL 1 can not decolorize the anthraquinone dye when it is the sole carbon source. Microbial cometabolism and decolorization of the dye take place in the presence of some other carbon source(0.2～0.4g/100ml)called cometabolic substrate. The cometabolic substrate can be peptone, glucose, sodium acetate, beef extract, amylum, etc. The change of molecular structure of the dye before and after decolorized by XL 1 is studied by UV Vis absorption spectrum. The results indicate that its molecular structure is changed evidently.

Wastewater Treatment for Removal of Recalcitrant Compounds：A Hybrid Process for Decolorization and Biodegradation of Dyes

While conventional wastewater treatments for urban effluents are fairly routine and have proved highly effective,industrial wastewater requires more complex and specific treatments.This paper provides a technological strategy for removal of recalcitrant contaminants based on a hybrid treatment system.The model effluent containing a binary mixture of synthetic dyes is treated by a combination of a preliminary physicochemical stage followed by a biological stage based on ligninolytic enzymes produced by Phanerochaete chrysosporium.This proposal includes biosorption onto peat as pretreatment,which decreases the volume and concentration to be treated in the biological reactor,thereby obtaining a completely decolorized effluent.The treated wastewater can therefore be reused in the dyeing baths with the consequent saving of water resources.

Biodegradation of Congo Red Azo Dye by Aspergillus spp, Isolated from Dye Contaminated Soils

In the present study, it has been aimed to determine the non target effects of Congo red on soil enzyme activities namely amylase, protease, catalase, glucose oxidase and laccases, following the standard procedures. Further, it is also aimed to decolourize the dye effluents by using immobilized fimgal cultures namely Aspergillus spp. isolated from textile effluents and estimate the percentage of decolourization by dye decolourization assay method. The selected fungi were found efficient and dominant type in dye decolourization with the production of laccase and other enzymes. Except laccase, all the enzymes showed maximum activity at 14 days followed by declining trend at 21 days. Maximum enzyme activity was noticed with Aspergillus niger. 95% of decolourization was found within 4-24 h in sodium alginate immobilized fungal cultures compared to controls. The above results clearly indicate the efficiency of these fungi in selective bioremediation of dye contaminated sites.