Objective: To elucidate the relation between human tissue factor pathwayinhibitor-2 (TFPI-2) expression and ovarian tumor migration and invasion. Methods: Human TFPI-2expression vector pBos-Cite-neo/TFPI-2 was transfe...Objective: To elucidate the relation between human tissue factor pathwayinhibitor-2 (TFPI-2) expression and ovarian tumor migration and invasion. Methods: Human TFPI-2expression vector pBos-Cite-neo/TFPI-2 was transfected into ovarian tumor cells line A2780- Afterthe transfected cells were selected by G418, transfected and nontransfected cells were screened forTFPI-2 mRNA and protein by reverse transcription-polymerase chain reaction and Western blotanalysis, respectively. The number of transfected or nontransfected cells passing through membraneof Boyden chamber was counted as the basis assessing tumor cells migratory and invasive behaviors.Results: Expression of mRNA and protein of TFPI-2 was detectable in transfected cells. In invasionassay, the number of TFPI-2-expressing cells to traverse a Matrigel-coated membrane was obviouslydecreased compared with that of nonexpressing cells (59.3±6.5 vs 109.7±5.5, P 【 0.01); While inmigration assay, no significant difference through a noncoated membrane was observed amongtransfected and nontransfected cells (114.7±8.6 vs 127.3±7.1, P 】 0.05). Conclusion: Expression ofTFPI-2 may strongly inhibit the invasive ability of ovarian tumor cells in vitro, but has no effecton the migratory ability which provides an experimental basis for genotherapy of human ovariantumor.展开更多
This study aimed to remediate in-situ leach mining contaminated soil by amendment-plant synergism. The results showed that plant species exhibited ex-tremely significant effects on the concentration of nitrate nitroge...This study aimed to remediate in-situ leach mining contaminated soil by amendment-plant synergism. The results showed that plant species exhibited ex-tremely significant effects on the concentration of nitrate nitrogen; to be specific, the concentration of nitrate nitrogen in soil planted with wheat was reduced from 692.19 mg/kg to lower than 100 mg/kg; when the mass ratio of amendment to soil reached 3:50 and the amendment particle size was 1-2 mm, the concentration of nitrate ni-trogen in soil planted with wheat was reduced to 43 mg/kg. The amendment type exhibited extremely significant effects on the concentration of ammonium nitrogen; to be specific, when the mass ratio of amendment to soil reached 10:50, the concen-tration of ammonium nitrogen in soil added with 2-3 mm zeolite was reduced from 23 593.75 to 3 300 mg/kg on day 15. Amendments and plants mainly exhibited desorption performance for sulfate radical in soil, and the amendment type extreme-ly significantly affected the concentration of sulfate radical; to be specific, the con-centration of sulfate radical in soil added with limestone increased from 370 mg/kg to 900 mg/kg on day 7.展开更多
Pb2+ and Cd2+ in leachate were adsorbed on clay-solidified grouting curtain for waste landfills with equilibrium experiment. The cation exchange capacity was determined with ammonium acetate. And the concentration of ...Pb2+ and Cd2+ in leachate were adsorbed on clay-solidified grouting curtain for waste landfills with equilibrium experiment. The cation exchange capacity was determined with ammonium acetate. And the concentration of heavy metal cations in leachate was determined with atomic absorption spectrophotometer. Their equilibrium isotherms were measured, and the experimental isotherm data were analyzed by using Freundlich and Langmuir models. The results show that the adsorption capacities of the heavy metal cations are closely related to the compositions of clay-solidified grouting curtain, and the maximum adsorption appears at the ratio of cement to clay of 2∶4 in the experimental conditions. At their maximum adsorption and pH 5.0, the adsorption capacities of Pb 2+ and Cd 2+ are 16.19mg/g and 1.21mg/g. The competitive adsorption coefficients indicate that the adsorption of clay-solidified grouting curtain for Pb2+ is stronger than that for Cd 2+ . The adsorption process conforms to Freundlich’s model with related coefficient higher than 0.996.展开更多
The serious limitations of available technologies for decontamination of wastewater have compelled researchers to search for alternative solutions. Catalytic treatment with hydrogen peroxide, which appears to be one o...The serious limitations of available technologies for decontamination of wastewater have compelled researchers to search for alternative solutions. Catalytic treatment with hydrogen peroxide, which appears to be one of the most efficient treatment systems, is able to degrade various organics with the help of powerful ·OH radicals. This review focuses on recent progress in the use of bicarbonate activated hydrogen peroxide for wastewater treatment. The introduction of bicarbonate to pollutant treatment has led to appreciable improvements, not only in process efficiency, but also in process stability. This review describes in detail the applications of this process in homogeneous and heterogeneous systems. The enhanced degradation, limited or lack of leaching during heterogeneous degradation, and prolonged catalysts stability during degradation are salient features of this system. This review provides readers with new knowledge regarding bicarbonate, including the fact that it does not always harm pollutant degradation, and can significantly benefit degradation under some conditions.展开更多
Bioleaching is an environment-friendly and economical technique to remove heavy metals from contaminated soil.The objective of this work is to find out an indigenous strain to remedy soil contaminated by Zn,Pb,Cu and ...Bioleaching is an environment-friendly and economical technique to remove heavy metals from contaminated soil.The objective of this work is to find out an indigenous strain to remedy soil contaminated by Zn,Pb,Cu and Cd.A strain which was selected from the soil of a local smelting industry was found to be able to produce many organic acids and degrade pH value of the liquid medium.The fungus strain is identified as Penicillium Chrysogenum (P.Chrysogenum) by sequencing 18srDNA and ITS.Bioleaching condition using P.Chrysogenum is optimized.Glucose is the best carbon source for P.Chrysogenum and inorganic nitrogen is better than organic nitrogen.In addition,neutral solution and room temperature are fit for P.Chrysogenum to bioleach.In the one-step bioleaching,the bioleaching ratios are 39.95% for Zn,9.4% for Pb,34.89% for Cu and 49.59% for Cd,which are 53.89% for Zn,14.44% for Pb,55.53% for Cu and 62.81% for Cd in the two-step bioleaching.The efficiency of two-step bioleaching is better than the one-step bioleaching.P.Chrysogenum is effective in removing heavy metals from the contaminated soil.展开更多
The authors investigated the leaching rate of antifouling agents from marine paint using an instrument that allows direct measurement from the flat bottom of ship hulls shortly after dry dock. A similar procedure was ...The authors investigated the leaching rate of antifouling agents from marine paint using an instrument that allows direct measurement from the flat bottom of ship hulls shortly after dry dock. A similar procedure was also used for painted flat panels that were immersed in the ocean. The relationship between leaching rate and fouling condition was considered by quantifying the amount of ATP (adenosine triphosphate) on the surface of immersed flat panel using fluorescence spectroscopy. The leaching rate results from ship hulls showed good agreement with the value by reporting of leaching rate of antifouling agents. The fluorescence spectroscopy results showed that the amount of ATP on the surface of the panel increased over time as the leaching rate fell, which implies an inverse relationship between leaching rate and fouling index. Fluorescence spectroscopy was also seen to be a simple method for evaluating the relationship between biofouling indexes and leaching rate.展开更多
The results of investigation of micromineralogy of disseminated-sulphide gold ore deposits of the Yana-Kolyma belt; the investigation was conducted via electron microscopy using a QEMSCAN (Quantitative Evaluation of ...The results of investigation of micromineralogy of disseminated-sulphide gold ore deposits of the Yana-Kolyma belt; the investigation was conducted via electron microscopy using a QEMSCAN (Quantitative Evaluation of Minerals by Scanning Electron Microscopy) hardware and software instrument equipped with a Quantax quantitative analysis system. The following micro-mineral phases have been detected: native gold and silver, selenides of Au and Ag (naumanite and fishesserite (?), hessite, sulfoarsenides of Ni and Co (gersdorffite, cobaltite, rammelsbergite), molibdenite and platinum group minerals. Our findings in disseminated sulfide ores selenides of Au and Ag, and molybdenite, together with the findings of platinum group minerals and Ni and Co, emphasize the "black shale" specifics character of these types of deposits.展开更多
AIM: To evaluate the predictive value of neutrophil in- filtration as a marker of Helicobacter pylori (H. pyloni) infection. METHODS: A total of 315 patients with dyspepsia symptoms who underwent upper gastrointes...AIM: To evaluate the predictive value of neutrophil in- filtration as a marker of Helicobacter pylori (H. pyloni) infection. METHODS: A total of 315 patients with dyspepsia symptoms who underwent upper gastrointestinal en- doscopy were enrolled in this study. Biopsies were evaluated using the updated Sydney system. The medication history of all patients in the preceding 4 wk was recorded. The diagnosis of H. pylori infection was based on 13C-urea breath test at least 4 wk after with- drawal of antisecretory drugs, antibiotics and related drugs. For the patients with subtotal gastrectomy, the diagnosis of H. pylori infection was based on anti-H. pylori immunoglobulin G (IgG) antibody. Serum anti-H. pylori IgG antibody was measured by enzyme-linked immunosorbent assays (Biohit, Finland). RESULTS: The sensitivity, specificity, positive predic- tive value and negative predictive value of neutrophil infiltration in the diagnosis ofH, pylorlinfection were 92.3%, 83.5%, 77.4% and 94.7%, respectively. Neu- trophil infiltration of gastric mucosa in the histological analysis was strongly associated withH, pylorlinfection (77.4% vs 5.3% in the neutrophil infiltration negative group, P = 0.000). Moderate neutrophil infiltration was more frequent in H. pylorl infection when compared to mild infiltration (81.8% and 75%, respectively), but did not reach statistical significance. For those patients with negative rapid urease test, H. pylori was detected in 73.2% of patients with positive neutrophil infiltration on histology. In patients with subtotal gastrectomy, the diagnostic accuracy of neutrophil infiltration in H. pylori infection was 50%. CONCLUSION: Neutrophil infiltration is closely associ- ated withH, pylori and may be recognized as a sign of this infection.展开更多
AIM: To investigate the changing pattern of different histological parameters occurring in the stomach tissue of Helicobacter pylori (H pylori) infected tribal populations and duodenal ulcer patients among ethnic B...AIM: To investigate the changing pattern of different histological parameters occurring in the stomach tissue of Helicobacter pylori (H pylori) infected tribal populations and duodenal ulcer patients among ethnic Bengalis and correlation of the genotypes of H pylori with different histological parameters. METHODS: One hundred and twelve adult individuals were enrolled into this study between 2002 and 2004. Among them, 72 had clinical features of duodenal ulcer (DU) from ethnic Bengali population and 40 were asymptomatic ethnic tribals. Endoscopic gastric biopsy samples were processed for histology, genotyping and rapid urease test. Histologically, haematoxylin and eosin staining was applied to assess the pathomorphological changes and a modified Giemsa staining was used for better detection of Hpylori. For intestinal metaplasia, special stainings, i.e. Alcian blue periodic acid-Schiff and high iron diamine-Alcian blue staining, were performed. PCR was performed on bacterial DNA to characterize the presence or absence of virulence-associated genes, like cagA, and distribution of different alleles of vacA and iceA. RESULTS: Intraglandular neutrophil infiltration, a hallmark of activity of gastritis, was present in 34 (94%) of tribals (TRs) and 42 (84%) of DU individuals infected with H pylori. Lymphoid follicles and aggregates, which are important landmarks in H pylori infection, were positive amongst 15 (41%) of TRs and 20 (40%) of DU subjects. Atrophic changes were observed in 60% and 27.7%, respectively, among DU cases and tribals (P 〉 0.003). Metaplastic changes were detected in low numbers in both groups. Moderate to severe density distribution of Hpylori in the gastric mucosa was 63% among TRs, whereas it was 62% in DU subjects. There were no significant differences in the distribution of virulence-associated genes like cagA, vacA and iceA of H pylori strains carried by these two populations. CONCLUSION: Our study showed almost similar distribution of inflammatory cells among asymptomatic tribals and DU Bengali patients. Interestingly, the tribal population are free from any clinical symptoms despite evidence of active histologic gastritis and infection with Hpylori strains carrying similar virulence markers as of strains isolated from patients with DU. There was an increased cellular response, especially in terms of neutrophil infiltration, but much lower risk of developing atrophy and metaplastic changes among the tribal population.展开更多
In order to improve the efficiency of bioleaching heavy metal from the contaminated soil using Penicillium chrysogenum(P.chrysogenum),experiment was conducted to evaluate the influence of heavy metal stress on P.chrys...In order to improve the efficiency of bioleaching heavy metal from the contaminated soil using Penicillium chrysogenum(P.chrysogenum),experiment was conducted to evaluate the influence of heavy metal stress on P.chrysogenum during bioleaching.The morphology and physiology of P.chrysogenum were observed.Assuming that the heavy metals are all leached out from the experiment soil,heavy metals are added into the agar medium by simulating the heavy metal content in the soil.It is concluded that the survivable heavy metal contaminated soil mass range for P.chrysogenum is 2.5-5.0 g.As for biomass determination,the contaminated soil is added into the liquid medium directly.The soil mass that P.chrysogenum can be survivable is in the range of 2.5-8.75 g.In this mass range,the biomass of P.chrysogenum is bigger than that of the control sample.10 g soil mass is the threshold of the growth of P.chrysogenum.102.2 mg/L gluconic acid,156.4 mg/L oxalic acid,191.6 mg/L pyruvic acid,0.02 mg/L citric acid,0.03 mg/L malic acid and 70.6 mg/L succinic acid are determined after 15 d bioleaching.The mycelium is broken into fragments,and heavy metals are adsorbed on the cell wall or transported into the cytoplasm during bioleaching.The GOD activity declines from 1.08 U/mL to 0.2 U/mL under 400 mg/L of multi-metal stress.The influence of Pb on GOD activity is bigger than that of Cr and Cd,and the GOD activity is not influenced apparently by Mn,Zn and Cu.展开更多
An HIV infection model with saturated infection rate and double delays is investigated. First, the existence of the infection-free equilibrium E0, the immune-exhausted equilibrium E1 and the infected equilibrium E2 wi...An HIV infection model with saturated infection rate and double delays is investigated. First, the existence of the infection-free equilibrium E0, the immune-exhausted equilibrium E1 and the infected equilibrium E2 with immunity in different conditions is shown. By analyzing the characteristic equation, we study the locally asymptotical stability of the trivial equilibrium, and the existence of Hopf bifurcations when two delays are used as the bifurcation parameter. Furthermore, we apply the Nyquist criterion to estimate the length of delay for which stability continues to hold. Then with suitable Lyapunov function and LaSalle's invariance principle, the global stability of the three equilibriums is obtained. Finally, numerical simulations are presented to illustrate the main mathematical results.展开更多
文摘Objective: To elucidate the relation between human tissue factor pathwayinhibitor-2 (TFPI-2) expression and ovarian tumor migration and invasion. Methods: Human TFPI-2expression vector pBos-Cite-neo/TFPI-2 was transfected into ovarian tumor cells line A2780- Afterthe transfected cells were selected by G418, transfected and nontransfected cells were screened forTFPI-2 mRNA and protein by reverse transcription-polymerase chain reaction and Western blotanalysis, respectively. The number of transfected or nontransfected cells passing through membraneof Boyden chamber was counted as the basis assessing tumor cells migratory and invasive behaviors.Results: Expression of mRNA and protein of TFPI-2 was detectable in transfected cells. In invasionassay, the number of TFPI-2-expressing cells to traverse a Matrigel-coated membrane was obviouslydecreased compared with that of nonexpressing cells (59.3±6.5 vs 109.7±5.5, P 【 0.01); While inmigration assay, no significant difference through a noncoated membrane was observed amongtransfected and nontransfected cells (114.7±8.6 vs 127.3±7.1, P 】 0.05). Conclusion: Expression ofTFPI-2 may strongly inhibit the invasive ability of ovarian tumor cells in vitro, but has no effecton the migratory ability which provides an experimental basis for genotherapy of human ovariantumor.
基金Supported by National Natural Science Foundation of China(Grant No.21067003,5136-4015)Natural Science Foundation of Jiangxi Province(Grant No.20114BAB203024)National High-Tech Research and Development Program of China(Grant No.2012BAC11B07)~~
文摘This study aimed to remediate in-situ leach mining contaminated soil by amendment-plant synergism. The results showed that plant species exhibited ex-tremely significant effects on the concentration of nitrate nitrogen; to be specific, the concentration of nitrate nitrogen in soil planted with wheat was reduced from 692.19 mg/kg to lower than 100 mg/kg; when the mass ratio of amendment to soil reached 3:50 and the amendment particle size was 1-2 mm, the concentration of nitrate ni-trogen in soil planted with wheat was reduced to 43 mg/kg. The amendment type exhibited extremely significant effects on the concentration of ammonium nitrogen; to be specific, when the mass ratio of amendment to soil reached 10:50, the concen-tration of ammonium nitrogen in soil added with 2-3 mm zeolite was reduced from 23 593.75 to 3 300 mg/kg on day 15. Amendments and plants mainly exhibited desorption performance for sulfate radical in soil, and the amendment type extreme-ly significantly affected the concentration of sulfate radical; to be specific, the con-centration of sulfate radical in soil added with limestone increased from 370 mg/kg to 900 mg/kg on day 7.
基金Project (200065) supported by University Key Teacher Foundation of the Ministry of Education of China
文摘Pb2+ and Cd2+ in leachate were adsorbed on clay-solidified grouting curtain for waste landfills with equilibrium experiment. The cation exchange capacity was determined with ammonium acetate. And the concentration of heavy metal cations in leachate was determined with atomic absorption spectrophotometer. Their equilibrium isotherms were measured, and the experimental isotherm data were analyzed by using Freundlich and Langmuir models. The results show that the adsorption capacities of the heavy metal cations are closely related to the compositions of clay-solidified grouting curtain, and the maximum adsorption appears at the ratio of cement to clay of 2∶4 in the experimental conditions. At their maximum adsorption and pH 5.0, the adsorption capacities of Pb 2+ and Cd 2+ are 16.19mg/g and 1.21mg/g. The competitive adsorption coefficients indicate that the adsorption of clay-solidified grouting curtain for Pb2+ is stronger than that for Cd 2+ . The adsorption process conforms to Freundlich’s model with related coefficient higher than 0.996.
基金supported by the National Natural Science Foundation of China(21273086)~~
文摘The serious limitations of available technologies for decontamination of wastewater have compelled researchers to search for alternative solutions. Catalytic treatment with hydrogen peroxide, which appears to be one of the most efficient treatment systems, is able to degrade various organics with the help of powerful ·OH radicals. This review focuses on recent progress in the use of bicarbonate activated hydrogen peroxide for wastewater treatment. The introduction of bicarbonate to pollutant treatment has led to appreciable improvements, not only in process efficiency, but also in process stability. This review describes in detail the applications of this process in homogeneous and heterogeneous systems. The enhanced degradation, limited or lack of leaching during heterogeneous degradation, and prolonged catalysts stability during degradation are salient features of this system. This review provides readers with new knowledge regarding bicarbonate, including the fact that it does not always harm pollutant degradation, and can significantly benefit degradation under some conditions.
基金Project(2009ZX07212-001-01) supported by Major Science and Technology Program for Water Pollution Control and Treatment of ChinaProject(50925417) supported by the National Natural Science Funds for Distinguished Young Scholar in ChinaProjects(50830301,51074191) supported by the National Natural Science Foundation of China
文摘Bioleaching is an environment-friendly and economical technique to remove heavy metals from contaminated soil.The objective of this work is to find out an indigenous strain to remedy soil contaminated by Zn,Pb,Cu and Cd.A strain which was selected from the soil of a local smelting industry was found to be able to produce many organic acids and degrade pH value of the liquid medium.The fungus strain is identified as Penicillium Chrysogenum (P.Chrysogenum) by sequencing 18srDNA and ITS.Bioleaching condition using P.Chrysogenum is optimized.Glucose is the best carbon source for P.Chrysogenum and inorganic nitrogen is better than organic nitrogen.In addition,neutral solution and room temperature are fit for P.Chrysogenum to bioleach.In the one-step bioleaching,the bioleaching ratios are 39.95% for Zn,9.4% for Pb,34.89% for Cu and 49.59% for Cd,which are 53.89% for Zn,14.44% for Pb,55.53% for Cu and 62.81% for Cd in the two-step bioleaching.The efficiency of two-step bioleaching is better than the one-step bioleaching.P.Chrysogenum is effective in removing heavy metals from the contaminated soil.
文摘The authors investigated the leaching rate of antifouling agents from marine paint using an instrument that allows direct measurement from the flat bottom of ship hulls shortly after dry dock. A similar procedure was also used for painted flat panels that were immersed in the ocean. The relationship between leaching rate and fouling condition was considered by quantifying the amount of ATP (adenosine triphosphate) on the surface of immersed flat panel using fluorescence spectroscopy. The leaching rate results from ship hulls showed good agreement with the value by reporting of leaching rate of antifouling agents. The fluorescence spectroscopy results showed that the amount of ATP on the surface of the panel increased over time as the leaching rate fell, which implies an inverse relationship between leaching rate and fouling index. Fluorescence spectroscopy was also seen to be a simple method for evaluating the relationship between biofouling indexes and leaching rate.
文摘The results of investigation of micromineralogy of disseminated-sulphide gold ore deposits of the Yana-Kolyma belt; the investigation was conducted via electron microscopy using a QEMSCAN (Quantitative Evaluation of Minerals by Scanning Electron Microscopy) hardware and software instrument equipped with a Quantax quantitative analysis system. The following micro-mineral phases have been detected: native gold and silver, selenides of Au and Ag (naumanite and fishesserite (?), hessite, sulfoarsenides of Ni and Co (gersdorffite, cobaltite, rammelsbergite), molibdenite and platinum group minerals. Our findings in disseminated sulfide ores selenides of Au and Ag, and molybdenite, together with the findings of platinum group minerals and Ni and Co, emphasize the "black shale" specifics character of these types of deposits.
基金Supported by The grant for Key Laboratory of Gastroenterology and Hepatology,Ministry of Health,Renji Hospital,Shanghai Jiao Tong University School of MedicineNational Natural Science Foundation of China,No.81170355
文摘AIM: To evaluate the predictive value of neutrophil in- filtration as a marker of Helicobacter pylori (H. pyloni) infection. METHODS: A total of 315 patients with dyspepsia symptoms who underwent upper gastrointestinal en- doscopy were enrolled in this study. Biopsies were evaluated using the updated Sydney system. The medication history of all patients in the preceding 4 wk was recorded. The diagnosis of H. pylori infection was based on 13C-urea breath test at least 4 wk after with- drawal of antisecretory drugs, antibiotics and related drugs. For the patients with subtotal gastrectomy, the diagnosis of H. pylori infection was based on anti-H. pylori immunoglobulin G (IgG) antibody. Serum anti-H. pylori IgG antibody was measured by enzyme-linked immunosorbent assays (Biohit, Finland). RESULTS: The sensitivity, specificity, positive predic- tive value and negative predictive value of neutrophil infiltration in the diagnosis ofH, pylorlinfection were 92.3%, 83.5%, 77.4% and 94.7%, respectively. Neu- trophil infiltration of gastric mucosa in the histological analysis was strongly associated withH, pylorlinfection (77.4% vs 5.3% in the neutrophil infiltration negative group, P = 0.000). Moderate neutrophil infiltration was more frequent in H. pylorl infection when compared to mild infiltration (81.8% and 75%, respectively), but did not reach statistical significance. For those patients with negative rapid urease test, H. pylori was detected in 73.2% of patients with positive neutrophil infiltration on histology. In patients with subtotal gastrectomy, the diagnostic accuracy of neutrophil infiltration in H. pylori infection was 50%. CONCLUSION: Neutrophil infiltration is closely associ- ated withH, pylori and may be recognized as a sign of this infection.
基金Supported by The Indian Council of Medical Research, Government of India and Program of Founding Research Center for Emerging and Reemerging Infectious Diseases, Ministry of Education, Culture, Sports, Science and Technology of Japan
文摘AIM: To investigate the changing pattern of different histological parameters occurring in the stomach tissue of Helicobacter pylori (H pylori) infected tribal populations and duodenal ulcer patients among ethnic Bengalis and correlation of the genotypes of H pylori with different histological parameters. METHODS: One hundred and twelve adult individuals were enrolled into this study between 2002 and 2004. Among them, 72 had clinical features of duodenal ulcer (DU) from ethnic Bengali population and 40 were asymptomatic ethnic tribals. Endoscopic gastric biopsy samples were processed for histology, genotyping and rapid urease test. Histologically, haematoxylin and eosin staining was applied to assess the pathomorphological changes and a modified Giemsa staining was used for better detection of Hpylori. For intestinal metaplasia, special stainings, i.e. Alcian blue periodic acid-Schiff and high iron diamine-Alcian blue staining, were performed. PCR was performed on bacterial DNA to characterize the presence or absence of virulence-associated genes, like cagA, and distribution of different alleles of vacA and iceA. RESULTS: Intraglandular neutrophil infiltration, a hallmark of activity of gastritis, was present in 34 (94%) of tribals (TRs) and 42 (84%) of DU individuals infected with H pylori. Lymphoid follicles and aggregates, which are important landmarks in H pylori infection, were positive amongst 15 (41%) of TRs and 20 (40%) of DU subjects. Atrophic changes were observed in 60% and 27.7%, respectively, among DU cases and tribals (P 〉 0.003). Metaplastic changes were detected in low numbers in both groups. Moderate to severe density distribution of Hpylori in the gastric mucosa was 63% among TRs, whereas it was 62% in DU subjects. There were no significant differences in the distribution of virulence-associated genes like cagA, vacA and iceA of H pylori strains carried by these two populations. CONCLUSION: Our study showed almost similar distribution of inflammatory cells among asymptomatic tribals and DU Bengali patients. Interestingly, the tribal population are free from any clinical symptoms despite evidence of active histologic gastritis and infection with Hpylori strains carrying similar virulence markers as of strains isolated from patients with DU. There was an increased cellular response, especially in terms of neutrophil infiltration, but much lower risk of developing atrophy and metaplastic changes among the tribal population.
基金Project(50925417)supported by the National Natural Science Foundation of China for Distinguished Young ScholarsProject(51074191)supported by the National Natural Science Foundation of ChinaProject(2012BAC09B04)supported by the National Key Technology Research and Development Program of China
文摘In order to improve the efficiency of bioleaching heavy metal from the contaminated soil using Penicillium chrysogenum(P.chrysogenum),experiment was conducted to evaluate the influence of heavy metal stress on P.chrysogenum during bioleaching.The morphology and physiology of P.chrysogenum were observed.Assuming that the heavy metals are all leached out from the experiment soil,heavy metals are added into the agar medium by simulating the heavy metal content in the soil.It is concluded that the survivable heavy metal contaminated soil mass range for P.chrysogenum is 2.5-5.0 g.As for biomass determination,the contaminated soil is added into the liquid medium directly.The soil mass that P.chrysogenum can be survivable is in the range of 2.5-8.75 g.In this mass range,the biomass of P.chrysogenum is bigger than that of the control sample.10 g soil mass is the threshold of the growth of P.chrysogenum.102.2 mg/L gluconic acid,156.4 mg/L oxalic acid,191.6 mg/L pyruvic acid,0.02 mg/L citric acid,0.03 mg/L malic acid and 70.6 mg/L succinic acid are determined after 15 d bioleaching.The mycelium is broken into fragments,and heavy metals are adsorbed on the cell wall or transported into the cytoplasm during bioleaching.The GOD activity declines from 1.08 U/mL to 0.2 U/mL under 400 mg/L of multi-metal stress.The influence of Pb on GOD activity is bigger than that of Cr and Cd,and the GOD activity is not influenced apparently by Mn,Zn and Cu.
基金This work was supported by National Natural Science Foundation of China (61174209, 11471034).
文摘An HIV infection model with saturated infection rate and double delays is investigated. First, the existence of the infection-free equilibrium E0, the immune-exhausted equilibrium E1 and the infected equilibrium E2 with immunity in different conditions is shown. By analyzing the characteristic equation, we study the locally asymptotical stability of the trivial equilibrium, and the existence of Hopf bifurcations when two delays are used as the bifurcation parameter. Furthermore, we apply the Nyquist criterion to estimate the length of delay for which stability continues to hold. Then with suitable Lyapunov function and LaSalle's invariance principle, the global stability of the three equilibriums is obtained. Finally, numerical simulations are presented to illustrate the main mathematical results.