目的探讨间期核荧光原位杂交(FISH)检测肺癌印片标本染色体异倍体的可行性和其临床应用前景。方法选取7、8、9和12号染色体特异着丝粒 DNA 探针,用 FISH 技术检测46例肺癌印片标本的染色体异倍体。结果 46例肺癌印片标本均检出染色体异...目的探讨间期核荧光原位杂交(FISH)检测肺癌印片标本染色体异倍体的可行性和其临床应用前景。方法选取7、8、9和12号染色体特异着丝粒 DNA 探针,用 FISH 技术检测46例肺癌印片标本的染色体异倍体。结果 46例肺癌印片标本均检出染色体异倍体,主要特征为7、8、和12号染色休增多及9号染色体丢失。7、8、9和12号染色体异倍体总检出率分别为:67.4%(31/46)、60.9%(28/46)、28.3%(13/46)和56.5%(26/31)。结论间期核 FISH 可以检测出肺癌印片标本中染色体异倍体,这些改变有望应用于肺癌早期诊断和预后判断。展开更多
Gossypium hirsutum L. and G. barbadense L. are the two cultivated tetraploid species of cotton. The first is characterized by a high yield and wide adaptation, and the second by its super fiber property. Substit...Gossypium hirsutum L. and G. barbadense L. are the two cultivated tetraploid species of cotton. The first is characterized by a high yield and wide adaptation, and the second by its super fiber property. Substitution line in which a pair of intact chromosomes of TM_1 ( G. hirsutum ) were replaced by a pair of homozygous chromosomes of 3_79 ( G. barbadense ) is an excellent material for genetic research and molecular tagging. In this study, substitution line 16 (Sub 16) was used to evaluate the performance of the 16th chromosome in G. barbadense in TM_1 background. The genetic analysis using the major gene plus polygene mixed inheritance model in F 2∶3 family revealed that there might exist 2 QTLs respectively for boll size, lint percentage, lint index, fiber length and the first fruit branch node, 1 QTL for fiber elongation and flowering date, and no QTL for seed index, fiber strength and Micronaire in chromosome 16. However, 9 QTLs (LOD (logarithm of odds)≥3.0) controlling 6 quantitative traits were significantly identified in linkage group of chromosome 16 constructed in (TM_1×3_79) F 2by interval mapping. Among them, 1 QTL for boll size, fiber length, flowering date and fiber elongation could explain 15.2%, 19.7%, 12.1%, and 11.7% phenotypic variance respectively, 2 QTLs for lint index could explain 11.6% and 41.9%, and 3 QTLs for lint percentage could explain 8.7%, 9.6% and 29.2% phenotypic variance respectively. One unlinked SSR marker was associated with one QTL respectively for boll size and flowering date and they could explain 1.60% and 4.63% phenotypic variance. The traits associated significantly with chromosome 16 from Sub 16 were boll weight, lint percentage, lint index, fiber length, fiber elongation and flowering days.展开更多
文摘目的探讨间期核荧光原位杂交(FISH)检测肺癌印片标本染色体异倍体的可行性和其临床应用前景。方法选取7、8、9和12号染色体特异着丝粒 DNA 探针,用 FISH 技术检测46例肺癌印片标本的染色体异倍体。结果 46例肺癌印片标本均检出染色体异倍体,主要特征为7、8、和12号染色休增多及9号染色体丢失。7、8、9和12号染色体异倍体总检出率分别为:67.4%(31/46)、60.9%(28/46)、28.3%(13/46)和56.5%(26/31)。结论间期核 FISH 可以检测出肺癌印片标本中染色体异倍体,这些改变有望应用于肺癌早期诊断和预后判断。
文摘Gossypium hirsutum L. and G. barbadense L. are the two cultivated tetraploid species of cotton. The first is characterized by a high yield and wide adaptation, and the second by its super fiber property. Substitution line in which a pair of intact chromosomes of TM_1 ( G. hirsutum ) were replaced by a pair of homozygous chromosomes of 3_79 ( G. barbadense ) is an excellent material for genetic research and molecular tagging. In this study, substitution line 16 (Sub 16) was used to evaluate the performance of the 16th chromosome in G. barbadense in TM_1 background. The genetic analysis using the major gene plus polygene mixed inheritance model in F 2∶3 family revealed that there might exist 2 QTLs respectively for boll size, lint percentage, lint index, fiber length and the first fruit branch node, 1 QTL for fiber elongation and flowering date, and no QTL for seed index, fiber strength and Micronaire in chromosome 16. However, 9 QTLs (LOD (logarithm of odds)≥3.0) controlling 6 quantitative traits were significantly identified in linkage group of chromosome 16 constructed in (TM_1×3_79) F 2by interval mapping. Among them, 1 QTL for boll size, fiber length, flowering date and fiber elongation could explain 15.2%, 19.7%, 12.1%, and 11.7% phenotypic variance respectively, 2 QTLs for lint index could explain 11.6% and 41.9%, and 3 QTLs for lint percentage could explain 8.7%, 9.6% and 29.2% phenotypic variance respectively. One unlinked SSR marker was associated with one QTL respectively for boll size and flowering date and they could explain 1.60% and 4.63% phenotypic variance. The traits associated significantly with chromosome 16 from Sub 16 were boll weight, lint percentage, lint index, fiber length, fiber elongation and flowering days.
