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一种相对快速收敛的遗传算法的探讨
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作者 唐日照 齐吉泰 《现代电子技术》 2007年第18期76-77,共2页
GA作为一种新的全局优化搜索技术比其他搜索算法,优点明显;其不足之处是当搜索具有复杂染色体结构的求解空间时收敛速度慢。针对这问题,提出了一种改进的相对快速收敛的GA算法的思路:增加对染色体的分割与重组操作,依据于各段的结构和段... GA作为一种新的全局优化搜索技术比其他搜索算法,优点明显;其不足之处是当搜索具有复杂染色体结构的求解空间时收敛速度慢。针对这问题,提出了一种改进的相对快速收敛的GA算法的思路:增加对染色体的分割与重组操作,依据于各段的结构和段长,组成段群体(segmented population),对其实施遗传操作以寻找优化段,重新组合成完整的染色体来搜索优化解。 展开更多
关键词 收敛速度 GA 染色体段 群体
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Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background 被引量:7
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作者 柴建芳 吴志明 +2 位作者 赵和 Andre LAROCHE 王海波 《Acta Botanica Sinica》 CSCD 2003年第4期379-383,共5页
为解决AFLP中样品间共同片段对多态性片段识别的干扰 ,作者建立了一种“减法AFLP”标记技术 ,并运用这种技术成功对小麦中的外源染色体片段进行了标记。运用该技术 ,样品间的共同扩增片段显著减少 ,在非变性聚丙稀酰胺凝胶上带型差异十... 为解决AFLP中样品间共同片段对多态性片段识别的干扰 ,作者建立了一种“减法AFLP”标记技术 ,并运用这种技术成功对小麦中的外源染色体片段进行了标记。运用该技术 ,样品间的共同扩增片段显著减少 ,在非变性聚丙稀酰胺凝胶上带型差异十分明显 ,多态性片段很容易分辨。其真实性通过将其中两条成功转换成外源染色体片段特异的SCAR标记得到了验证。减法AFLP标记技术的建立为标记作物中的外源染色体片段提供了一种有效手段。 展开更多
关键词 subtracted AFLP subtractive hybridization AFLP alien chromosome fragment WHEAT
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基于小生境遗传算法的连续属性关联规则挖掘 被引量:3
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作者 杨光军 王丽丽 《计算机工程与应用》 CSCD 北大核心 2008年第5期184-186,共3页
对连续属性数据进行关联规则提取是一个重要的课题,构造了一种新的遗传算法模型,在结构上采用三段式染色体,将连续属性离散化、属性约简和关联规则提取集成在一起,并将小生境引入到遗传算法中避免"早熟"现象。实验表明了该算... 对连续属性数据进行关联规则提取是一个重要的课题,构造了一种新的遗传算法模型,在结构上采用三段式染色体,将连续属性离散化、属性约简和关联规则提取集成在一起,并将小生境引入到遗传算法中避免"早熟"现象。实验表明了该算法是有效的。 展开更多
关键词 关联规则 遗传算法 离散化 结构染色体 小生境
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QTLs Mapping for Salinity Tolerance at Seedling Stage or Rice(Oryza sativa L.) Using Chromosome Segment Substitution Lines
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作者 林静 张所兵 +2 位作者 张云辉 汪迎节 方先文 《Agricultural Science & Technology》 CAS 2017年第12期2209-2211,共3页
In this study, a population of chromosome segment substitution lines (CSSLs) derived from the cross between 9311 (indica) and Nipponbare (japonica) was employed to map the quantitative trait loci (QTLs) for sa... In this study, a population of chromosome segment substitution lines (CSSLs) derived from the cross between 9311 (indica) and Nipponbare (japonica) was employed to map the quantitative trait loci (QTLs) for salt tolerance under the salt stress simulated with 0.5% NaCI, using survival rate as the index. The data were analyzed by QTL IciMapping v3.1, and the results showed that one QTL (QSsr3) related to salt tolerance was located in the vicinity of the marker RM1350 on chromosome 3, into a genetic interval of 113.2-132.8 cM, with a contribution rate of 17.75%. The additive effect was 10.9, indicating that the QTL derived from the parent Nipponbare improved the salt tolerance of rice at seedling stage. This study will provide a theoretical basis for the selection of salt tolerant rice germplasm. 展开更多
关键词 RICE Salt tolerance Chromosome segment substitution lines (CSSLs) Quantitative trait loci (QTLs) mapping
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Mapping QTLs Associated with Sheath Blight Resistance Using Chromosome Segment Substitution Lines of Rice(Oryza sativa L.)
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作者 林静 张所兵 +2 位作者 张云辉 汪迎节 方先文 《Agricultural Science & Technology》 CAS 2014年第5期756-759,共4页
In this study, a population of 119 chromosome segment substitution lines (CSSLs) derived from backcross between indica 9311 and japonica Nipponbare was employed to map quantitative trait loci (QTL) associated with... In this study, a population of 119 chromosome segment substitution lines (CSSLs) derived from backcross between indica 9311 and japonica Nipponbare was employed to map quantitative trait loci (QTL) associated with sheath blight resis-tance in rice with toothpick inoculation method. A total of three sheath blight resis-tance-associated QTLs (qsb8-1, qsb8-2 and qsb8-3) were identified, which were lo-cated on adjacent molecular markers RM3262, RM5485 and RM3496 of chromo-some 8; the genetic interval was 81.7cM-91.7cM, 91.7cM-108.1cM and 108.1cM-119.6cM, respectively. The additive effect of qsb8-2 was negative, indicating that sheath blight resistance of susceptible parent harboring qsb8-2 fragment was en-hanced; additive effects of qsb8-1 and qsb8-3 were positive, indicating that sheath blight resistance of susceptible parent harboring qsb8-1 and qsb8-3 fragments was reduced. 展开更多
关键词 RICE Sheath blight resistance Chromosome segment substitution lines(CSSLs) Quantitative trait locus (QTL) mapping
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Direct cloning and transplanting of large DNA fragments from Escherichia coli chromosome
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作者 Ying Zhu Yan Yang +3 位作者 Pingping Den Yong Huang Mengxiang Ni Hongqing Fang 《Science China(Life Sciences)》 SCIE CAS CSCD 2016年第10期1034-1041,共8页
We applied a resistance split-fusion strategy to increase the in vivo direct cloning efficiency mediated by Red recombination. The cat cassette was divided into two parts: cma (which has a homologous sequence with ... We applied a resistance split-fusion strategy to increase the in vivo direct cloning efficiency mediated by Red recombination. The cat cassette was divided into two parts: cma (which has a homologous sequence with cmb) and cmb, each of which has no resistance separately unless the two parts are fused together. The crab sequence was integrated into one flank of a target clon- ing region in the chromosome, and a linear vector containing the cma sequence was electroporated into the cells to directly capture the target region. Based on this strategy, we successfully cloned an approximately 48 kb DNA fragment from the E. coli DH1-Z chromosome with a positive frequency of approximately 80%. Combined with double-strand breakage-stimulated homologous recombination, we applied this strategy to successfully replace the corresponding region of the E. coli DH36 chromosome and knock out four non-essential genomic regions in one step. This strategy could provide a powerful tool for the heterologous expression of microbial natural product biosynthetic pathways for genome assembly and for the functional study of DNA sequences dozens of kilobases in length. 展开更多
关键词 Red homologous recombination resistance split-fusion target cloning transferring
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