流式细胞术(flow cytometry)可以实现高速、逐一的细胞定量分析和分选,是研究和诊断血液病的重要手段之一。但是由于不同实验所用细胞和实验条件不同,经常存在抗原阴性细胞非特异染色等问题。利用抗体滴定法,可通过计算、比较染色指数,...流式细胞术(flow cytometry)可以实现高速、逐一的细胞定量分析和分选,是研究和诊断血液病的重要手段之一。但是由于不同实验所用细胞和实验条件不同,经常存在抗原阴性细胞非特异染色等问题。利用抗体滴定法,可通过计算、比较染色指数,得到使抗原阳性细胞群和阴性细胞群达到最佳分离效果的实验条件。为了优化血液细胞流式细胞术中荧光抗体染色的实验条件,以小鼠骨髓细胞为被标记细胞,选择利用非串联荧光染料FITC标记的大鼠抗小鼠CD11b抗体(FITC Rat Anti-Mouse CD11b)和串联荧光染料APC-eFluor780标记的大鼠抗小鼠CD11b抗体(APC-eFluor780 Rat Anti-Mouse CD11b)进行标记。通过计算不同浓度抗体标记小鼠骨髓细胞的染色指数进行抗体滴定,确定合适的抗体浓度区间,进而分析细胞数量、染色时间及固定步骤对抗体染色指数的影响,探究影响血液细胞抗体染色的关键因素。结果显示,FITC Rat Anti-Mouse CD11b和APC-eFluor780 Rat Anti-Mouse CD11b的浓度分别在0.156~2.500μg·mL-1和0.25~1.00μg·mL-1范围内染色指数较高,但是超出这个范围的抗体浓度会使染色指数降低;抗体浓度、染色时间一定时,FITC Rat Anti-Mouse CD11b和APC-eFluor780 Rat Anti-Mouse CD11b分别在细胞数量为1.56×105~5.00×106 cells·管-1和1.56×105~3.12×105 cells·管-1范围内染色指数较高,但是超出这个范围的细胞数量会使染色指数降低;抗体浓度、细胞数量一定时,对于FITC Rat Anti-Mouse CD11b,随着染色时间的延长,染色指数降低,而APC-eFluor780 Rat Anti-Mouse CD11b与之相反;通过比较固定前后染色指数的高低发现,FITC Rat Anti-Mouse CD11b和APC-eFluor780 Rat Anti-Mouse CD11b在固定后染色指数均显著下降(P<0.01和P<0.05)。研究结果提供了一种通过抗体滴定优化流式分析血液细胞的方法,并指出在特定实验中根据抗体滴定结果选择合适的抗体浓度、细胞数量、染色时间和固定步骤对标记血液细胞进行流式检测的研究至关重要。展开更多
AIM： To investigate the expressions of leptin and per- oxisome proliferator-activated receptor y （PPARG） in relation to body mass index （BMI）. METHODS： We evaluated leptin and PPARG expres- sion in 30 adenomas o...AIM： To investigate the expressions of leptin and per- oxisome proliferator-activated receptor y （PPARG） in relation to body mass index （BMI）. METHODS： We evaluated leptin and PPARG expres- sion in 30 adenomas over 1 cm in size by immunohisto- chemical staining. In addition, clinicopathologic features including BMI were assessed. RESULTS： PPARG and leptin expression showed a strong positive correlation （P = 0.035）. The average BMI of the leptin-positive group was higher than that of the leptin-negative group （25.4 ＋ 3.4 kg/m2 vs 22.6 ＋ 2.4 kg/m2, P = 0.018）, and leptin expression was sig- nificantly correlated with high BMI （P = 0.024）. Leptinexpression was more frequently observed in intermedi- ate/high grade dysplasia than in low grade dysplasia （P = 0.030）. However, PPARG expression was not cor- related with BMI and grade of dysplasia. CONCLUSION： BMI has influenced on the leptin ex- pression of colorectal adenoma. The exact mechanism underlies the strong correlation between leptin and PPARG expression needs further study.展开更多
The new standards for the photometric characterization of light-emitting diode(LED)sources and luminaries require the measurement evaluation of photometric and colorimetric quantities.This present work studies the per...The new standards for the photometric characterization of light-emitting diode(LED)sources and luminaries require the measurement evaluation of photometric and colorimetric quantities.This present work studies the performance of some types of LED light sources in different spectra and different color temperature for photometric and colorimetric applications.The study depends on mixed spectra of these different types of LEDs to enhance the color rendering index(CRI)to be near as possible to standard sources.Characterization of LED sources requires the evaluation of some photometric and colorimetric quantities such as luminous flux(),chromaticity coordinates(x,y)and(u′,v′),correlated color temperature(CCT)and color rending indices(CRI).展开更多
The reaction ofAllium cepa root tips to the presence of potential toxic chemicals was used to evaluate the cyto/genotoxic effects of some water sources of Nen-Shkodra lowland agricultural area after massive 2010-2011 ...The reaction ofAllium cepa root tips to the presence of potential toxic chemicals was used to evaluate the cyto/genotoxic effects of some water sources of Nen-Shkodra lowland agricultural area after massive 2010-2011 flooding. MRL （Mean root length）, MI （mitotic index）, PI （phase index） and CA （chromosome abnormalities） endpoints of onion roots, grown in four water samples, were measured and compared with tap water and Cu solution 0.5 mg/L （negative and positive controls）. Inhibition of root growth, cell division and induction of mitotic and chromosomal aberrations were detected. The most polluted water sample caused MRL inhibition of 36%, MI decrease of 38%, and a considerable increase in chromosomal aberrations （7.8%） compared to the negative control. Most frequent CA types were： bridges and fragments, stickiness and C-metaphase. The results indicated a slight toxic tendency of analyzed natural waters, serving as an alert of environmental impact that chemical pollution had after flooding.展开更多
文摘流式细胞术(flow cytometry)可以实现高速、逐一的细胞定量分析和分选,是研究和诊断血液病的重要手段之一。但是由于不同实验所用细胞和实验条件不同,经常存在抗原阴性细胞非特异染色等问题。利用抗体滴定法,可通过计算、比较染色指数,得到使抗原阳性细胞群和阴性细胞群达到最佳分离效果的实验条件。为了优化血液细胞流式细胞术中荧光抗体染色的实验条件,以小鼠骨髓细胞为被标记细胞,选择利用非串联荧光染料FITC标记的大鼠抗小鼠CD11b抗体(FITC Rat Anti-Mouse CD11b)和串联荧光染料APC-eFluor780标记的大鼠抗小鼠CD11b抗体(APC-eFluor780 Rat Anti-Mouse CD11b)进行标记。通过计算不同浓度抗体标记小鼠骨髓细胞的染色指数进行抗体滴定,确定合适的抗体浓度区间,进而分析细胞数量、染色时间及固定步骤对抗体染色指数的影响,探究影响血液细胞抗体染色的关键因素。结果显示,FITC Rat Anti-Mouse CD11b和APC-eFluor780 Rat Anti-Mouse CD11b的浓度分别在0.156~2.500μg·mL-1和0.25~1.00μg·mL-1范围内染色指数较高,但是超出这个范围的抗体浓度会使染色指数降低;抗体浓度、染色时间一定时,FITC Rat Anti-Mouse CD11b和APC-eFluor780 Rat Anti-Mouse CD11b分别在细胞数量为1.56×105~5.00×106 cells·管-1和1.56×105~3.12×105 cells·管-1范围内染色指数较高,但是超出这个范围的细胞数量会使染色指数降低;抗体浓度、细胞数量一定时,对于FITC Rat Anti-Mouse CD11b,随着染色时间的延长,染色指数降低,而APC-eFluor780 Rat Anti-Mouse CD11b与之相反;通过比较固定前后染色指数的高低发现,FITC Rat Anti-Mouse CD11b和APC-eFluor780 Rat Anti-Mouse CD11b在固定后染色指数均显著下降(P<0.01和P<0.05)。研究结果提供了一种通过抗体滴定优化流式分析血液细胞的方法,并指出在特定实验中根据抗体滴定结果选择合适的抗体浓度、细胞数量、染色时间和固定步骤对标记血液细胞进行流式检测的研究至关重要。
基金Supported by Grant from Inje University College of Medicine(2010)
文摘AIM： To investigate the expressions of leptin and per- oxisome proliferator-activated receptor y （PPARG） in relation to body mass index （BMI）. METHODS： We evaluated leptin and PPARG expres- sion in 30 adenomas over 1 cm in size by immunohisto- chemical staining. In addition, clinicopathologic features including BMI were assessed. RESULTS： PPARG and leptin expression showed a strong positive correlation （P = 0.035）. The average BMI of the leptin-positive group was higher than that of the leptin-negative group （25.4 ＋ 3.4 kg/m2 vs 22.6 ＋ 2.4 kg/m2, P = 0.018）, and leptin expression was sig- nificantly correlated with high BMI （P = 0.024）. Leptinexpression was more frequently observed in intermedi- ate/high grade dysplasia than in low grade dysplasia （P = 0.030）. However, PPARG expression was not cor- related with BMI and grade of dysplasia. CONCLUSION： BMI has influenced on the leptin ex- pression of colorectal adenoma. The exact mechanism underlies the strong correlation between leptin and PPARG expression needs further study.
文摘The new standards for the photometric characterization of light-emitting diode(LED)sources and luminaries require the measurement evaluation of photometric and colorimetric quantities.This present work studies the performance of some types of LED light sources in different spectra and different color temperature for photometric and colorimetric applications.The study depends on mixed spectra of these different types of LEDs to enhance the color rendering index(CRI)to be near as possible to standard sources.Characterization of LED sources requires the evaluation of some photometric and colorimetric quantities such as luminous flux(),chromaticity coordinates(x,y)and(u′,v′),correlated color temperature(CCT)and color rending indices(CRI).
文摘The reaction ofAllium cepa root tips to the presence of potential toxic chemicals was used to evaluate the cyto/genotoxic effects of some water sources of Nen-Shkodra lowland agricultural area after massive 2010-2011 flooding. MRL （Mean root length）, MI （mitotic index）, PI （phase index） and CA （chromosome abnormalities） endpoints of onion roots, grown in four water samples, were measured and compared with tap water and Cu solution 0.5 mg/L （negative and positive controls）. Inhibition of root growth, cell division and induction of mitotic and chromosomal aberrations were detected. The most polluted water sample caused MRL inhibition of 36%, MI decrease of 38%, and a considerable increase in chromosomal aberrations （7.8%） compared to the negative control. Most frequent CA types were： bridges and fragments, stickiness and C-metaphase. The results indicated a slight toxic tendency of analyzed natural waters, serving as an alert of environmental impact that chemical pollution had after flooding.
