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乳腺癌易感基因BRCA1研究进展 被引量:2
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作者 严景华 叶棋浓 黄翠芬 《遗传》 CAS CSCD 北大核心 2004年第3期367-372,共6页
BRCA1是目前所发现的最重要的乳腺癌易感基因之一,它在DNA损伤修复,细胞周期调节,基因的转录激活,染色质稳定性,细胞增殖等方面都起着重要作用。该文着重介绍近几年来BRCA1基础研究方面的进展,并讨论BRCA1在肿瘤发生、发展过程的作用。... BRCA1是目前所发现的最重要的乳腺癌易感基因之一,它在DNA损伤修复,细胞周期调节,基因的转录激活,染色质稳定性,细胞增殖等方面都起着重要作用。该文着重介绍近几年来BRCA1基础研究方面的进展,并讨论BRCA1在肿瘤发生、发展过程的作用。为BRCA1在临床上的应用提供理论依据。 展开更多
关键词 BRCA1 DNA损伤修复 细胞周期调节 基因的转录激活 染色质稳定性
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Inhibition of SIRT1 Increases EZH2 Protein Level and Enhances the Repression of EZH2 on Target Gene Expression
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作者 Chih-chuan Liang 《Chinese Medical Sciences Journal》 CAS CSCD 2011年第2期77-84,共8页
Objective To study the regulatory roles of SIRT1 on EZH2 expression and the further ef-fects on EZH2's repression of target gene expression. Methods The stable SIRT1 RNAi and Control RNAi HeLa cells were establish... Objective To study the regulatory roles of SIRT1 on EZH2 expression and the further ef-fects on EZH2's repression of target gene expression. Methods The stable SIRT1 RNAi and Control RNAi HeLa cells were established by in-fection with retroviruses expressing shSIRT1 and shLuc respectively followed by puromycin selection. EZH2 protein level was detected by Western blot in either whole cell lysate or the fractional cell extract. Reverse transcription-polymerase chain reaction was performed to detect the mRNA level of EZH2. Cycloheximide was used to treat SIRT1 RNAi and Control RNAi cells for protein stability assay. Chromatin immunoprecipitation (ChIP) assay was applied to measure enrichment of SIRT1, EZH2, and trimethylated H3K27 (H3K27me3) at SATB1 promoter in SIRT1 RNAi and Control RNAi cells. Results Western blot results showed that EZH2 protein level increased upon SIRT1 de-pletion. Fractional extraction results showed unchanged cytoplasmic fraction and increased chromatin fraction of EZH2 protein in SIRT1 RNAi cells. The mRNA level of EZH2 was not affected by knockdown of SIRT1. SIRT1 recruitment was not detected at the promoter region of EZH2 gene locus. The protein stability assay showed that the protein stability of EZH2 increases upon SIRT1 knockdown. Upon SIRT1 depletion, EZH2 and H3K27me3 recruitment at SATB1 promoter increases and the mRNA level of SATB1 decreases. Conclusions Depletion of SIRT1 increases the protein stability of EZH2. The regulation of EZH2 protein level by SIRT1 affects the repressive effects of EZH2 on the target gene expres-sion. 展开更多
关键词 SIRT1 EZH2 Polycomb repression complex trimethylated H3K27
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Recruitment and reinforcement: maintaining epigenetic silencing 被引量:6
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作者 Chengzhi Wang Bing Zhu Jun Xiong 《Science China(Life Sciences)》 SCIE CAS CSCD 2018年第5期515-522,共8页
Cells need to appropriately balance transcriptional stability and adaptability in order to maintain their identities while responding robustly to various stimuli. Eukaryotic cells use an elegant "epigenetic"... Cells need to appropriately balance transcriptional stability and adaptability in order to maintain their identities while responding robustly to various stimuli. Eukaryotic cells use an elegant "epigenetic" system to achieve this functionality. "Epigenetics" is referred to as heritable information beyond the DNA sequence, including histone and DNA modifications, nc RNAs and other chromatin-related components. Here, we review the mechanisms of the epigenetic inheritance of a repressive chromatin state,with an emphasis on recent progress in the field. We emphasize that(i) epigenetic information is inherited in a relatively stable but imprecise fashion;(ii) multiple cis and trans factors are involved in the maintenance of epigenetic information during mitosis; and(iii) the maintenance of a repressive epigenetic state requires both recruitment and self-reinforcement mechanisms.These mechanisms crosstalk with each other and form interconnected feedback loops to shape a stable epigenetic system while maintaining certain degrees of flexibility. 展开更多
关键词 heterochromatin heterochromatin maintenance polycomb H3K27me3 H3K9me2/3
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Conservation and characterization of unique porcine interstitial telomeric sequences 被引量:1
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作者 JI GuangZhen LIU Kai +8 位作者 CHEN ChengBin RUAN WeiMin GLYTSOU Christina YANG Yang OKUKA Maja SONG WenQing GAGOS Sarantis LINing LIU Lin 《Science China(Life Sciences)》 SCIE CAS 2012年第12期1029-1037,共9页
Telomeres are composed of TTAGGG repeats and located at the ends of chromosomes. Telomeres protect chromosomes from instability in mammals, including mice and humans. Repetitive TTAGGG sequences are also found at intr... Telomeres are composed of TTAGGG repeats and located at the ends of chromosomes. Telomeres protect chromosomes from instability in mammals, including mice and humans. Repetitive TTAGGG sequences are also found at intrachromosomal sites, where they are named as interstitial telomeric sequences (ITSs). Aberrant ITSs are implicated in chromosomal instability and found in cancer cells. Interestingly, in pigs, vertebrate telomere sequences TTAGGG (vlTSs) are also localized at the centro- meric region of chromosome 6, in addition to the end of all chromosomes. Surprisingly, we found that botanic telomere se- quences, TTTAGGG (bITSs), also localize with vITSs at the centromeric regions of pig chromosome 6 using telomere fluo- rescence in situ hybridization (FISH) and by comparisons between several species. Furthermore, the average lengths of vITSs are highly correlated with those of the terminal telomeres (TTS). Also, pig ITSs show a high incidence of telomere doublets, suggesting that pig ITSs might be unstable and dynamic. Together, our results show that pig cells maintain the conserved te- lomere sequences that are found at the ITSs from of plants and other vertebrates. Further understanding of the function and regulation of pig ITSs may provide new clues for evolution and chromosomal instability. 展开更多
关键词 evolution TELOMERE interstitial telomeric sequence (ITS) PIG
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