The structure of the nuclosome core particle of chromatin in chicken erythrocytes has been examined by usingAFM. The 146 hp of DNA wrapped twice around the corehistone octamer are clearly visualized. Both the ends ofe...The structure of the nuclosome core particle of chromatin in chicken erythrocytes has been examined by usingAFM. The 146 hp of DNA wrapped twice around the corehistone octamer are clearly visualized. Both the ends ofentry/exit of linker DNA are also demonstrated. The dimension of the nucleosome core particles is- 1-4 um inheight and ～13-22 um in width. In addition, superbeads(width of - 48-57 urn, beight of-2-3 nm) are occasionallyrevealed, two turns of DNA around the core particles arealso detected.展开更多
Fine structure of the hypobranchial gland of Haliotis diversicolor has been studied by light and electron microscopy. The results showed that the gland is folded pleats organ, which is highly glandular area of the epi...Fine structure of the hypobranchial gland of Haliotis diversicolor has been studied by light and electron microscopy. The results showed that the gland is folded pleats organ, which is highly glandular area of the epidermal lining the roof of the mantle cavity. Two such glandular areas, one on each side of the rectum, occur in H. diversicolor. Left one is much larger than fight one. By light microscope, on H-E stained section, four cell types can be divided： cells with weak basophilic fibrillar elements; with acidophilic granular substance; with strong basophiIic fibrillar elements and ciliated ceils. In the basal lamina region under the gland epithelium, there are a few connective tissues. Surface view of the gland could be seen by scanning electron microscope, there are cilia and different kinds of secretions distributed. By transmission electron microscope, supporting cells, sensory cells and seven types gland cells were observed to form the glandular epithelium. Cells are rich in rough endoplasmic reticulum, smooth muscle fiber and nerve endings were found beneath glandular epithelium, between basal lamina.展开更多
Previous study on TNFRl-mediated hepatocyte apoptosis has been implicated in the development of fulminant viral hepatitis. To interfere with the potentially effective target, plasmid named p-mTNFRlshRNA complimentary ...Previous study on TNFRl-mediated hepatocyte apoptosis has been implicated in the development of fulminant viral hepatitis. To interfere with the potentially effective target, plasmid named p-mTNFRlshRNA complimentary to the sequence responsible for mTNFR1 was also constructed and further confirmed by sequence analysis. To investigate the effect of mTNFRlshRNA plasmid on mTNFR1 expression in vivo and the disease progress in MHV-3 induced fulminant hepatitis mice model. By hydrodynamic injection of mTNFRlshRNA plasmid, the survival rate of mice, hepatic pathological change were examined and compared between mice with/without mTNFRlshRNA plasmid intervention. The expression of mTNFR1 was detected by Real-time PCR, immunohistochemistry staining. The mTNFRlshRNA plasmid significantly reduced mTNFR1 expression in vivo, markedly ameliorates inflammatory infiltration, prolonged the survival time period and elevated the survival rate from 0 up to 13.3% in Balb/cJ mice with MHV-3 induced fulminant hepatitis. This study was designed to explore the opportunity of RNA interference technique in inhibiting TNFR1 expression, which has been reported to be involved in the development of a variety of diseases including fulminant viral hepatitis and severe chronic hepatitis B.展开更多
文摘The structure of the nuclosome core particle of chromatin in chicken erythrocytes has been examined by usingAFM. The 146 hp of DNA wrapped twice around the corehistone octamer are clearly visualized. Both the ends ofentry/exit of linker DNA are also demonstrated. The dimension of the nucleosome core particles is- 1-4 um inheight and ～13-22 um in width. In addition, superbeads(width of - 48-57 urn, beight of-2-3 nm) are occasionallyrevealed, two turns of DNA around the core particles arealso detected.
基金Acknowledgments： This study was founded by Natural Science Foundation of Guangdong （No. 032248） and Large Instrument Using Fund of South China Agricultural University （ No. 2007Y002 ） .
文摘Fine structure of the hypobranchial gland of Haliotis diversicolor has been studied by light and electron microscopy. The results showed that the gland is folded pleats organ, which is highly glandular area of the epidermal lining the roof of the mantle cavity. Two such glandular areas, one on each side of the rectum, occur in H. diversicolor. Left one is much larger than fight one. By light microscope, on H-E stained section, four cell types can be divided： cells with weak basophilic fibrillar elements; with acidophilic granular substance; with strong basophiIic fibrillar elements and ciliated ceils. In the basal lamina region under the gland epithelium, there are a few connective tissues. Surface view of the gland could be seen by scanning electron microscope, there are cilia and different kinds of secretions distributed. By transmission electron microscope, supporting cells, sensory cells and seven types gland cells were observed to form the glandular epithelium. Cells are rich in rough endoplasmic reticulum, smooth muscle fiber and nerve endings were found beneath glandular epithelium, between basal lamina.
基金National Science Fund of China(NSFC)(30571643,30672380,30700702)National Key Basic Research Program of China(2005CB522901,2007CB512900)
文摘Previous study on TNFRl-mediated hepatocyte apoptosis has been implicated in the development of fulminant viral hepatitis. To interfere with the potentially effective target, plasmid named p-mTNFRlshRNA complimentary to the sequence responsible for mTNFR1 was also constructed and further confirmed by sequence analysis. To investigate the effect of mTNFRlshRNA plasmid on mTNFR1 expression in vivo and the disease progress in MHV-3 induced fulminant hepatitis mice model. By hydrodynamic injection of mTNFRlshRNA plasmid, the survival rate of mice, hepatic pathological change were examined and compared between mice with/without mTNFRlshRNA plasmid intervention. The expression of mTNFR1 was detected by Real-time PCR, immunohistochemistry staining. The mTNFRlshRNA plasmid significantly reduced mTNFR1 expression in vivo, markedly ameliorates inflammatory infiltration, prolonged the survival time period and elevated the survival rate from 0 up to 13.3% in Balb/cJ mice with MHV-3 induced fulminant hepatitis. This study was designed to explore the opportunity of RNA interference technique in inhibiting TNFR1 expression, which has been reported to be involved in the development of a variety of diseases including fulminant viral hepatitis and severe chronic hepatitis B.
