浅谈朱砂染衣

染衣,俗称"挂衣",即将朱砂细粉按一定比例均匀地附在另一种药物表面的方法.中医学认为,朱砂染衣入煎可增强药物安神定惊、清心降火作用.但该法存在着种种弊端和隐患,不宜采用.

湖南省牛和猪衣原体病感染的血清学调查

应用间接血凝试验对湖南省的奶牛、水牛、黄牛和有流产史的母猪进行了农原体病的血清学检测;被查的410份奶牛血清有33份为阳性,37份为可疑;被查的111份猪血清有10份为阳性,3份为可疑;从受查血清中抽查176头奶牛和62头母猪血清作布氏杆菌病检测,阳性率分别为3.4％和25.80％,可疑率分别为9.09％和12.90％。

应用分散染料常温常压易染色涤纶开发匹染毛涤女衣呢

介绍了利用分散染料常温常压易染色涤纶 (EDDP)与羊毛同浴染色生产精纺毛涤女衣呢的情况。从产品规格设计 ,产品生产工艺流程等方面进行探讨 ,并对成品质量进行了分析。

精纺毛涤匹染女衣呢开发研制

介绍了利用分散染料常温常压易染色涤纶开发毛涤匹染女衣呢的情况。从新产品规格、生产工艺流程等方面进行探讨,并对成品质量进行分析。

马的衣原体感染

随着人们对衣原体研究的深入,鹦鹉衣原体所致畜禽疾病在许多国家和地区都有报道,其分布、危害日趋严重,并有传播的趋势。由于衣原体感染的多趋向性,畜禽患病时,很多组织和器官(肺、肝、肾、脑、胃、肠、呼吸道、尿生殖道、关节、角膜、结膜等)都可能被感染,因而本病临床症状显得特别复杂。马的衣原体病主要表现为母马的流产或产弱胎与死胎、肺炎、多关节炎、角膜结膜炎、肠炎、肝炎。在幼驹可引起支气管炎和多关节炎。严重者可引起死亡。一、历史与分布马属动物的衣原体病最早是由Popovici(1968)报道的,他从2匹患有支气管肺炎的马驹血液中分离出了衣原体。

Correlation of Chlamydia pneumonias infection with primary biliary cirrhosis

AIM:To evaluate the association between Chlamydia pneumoniae (Cpn) infection and primary biliary cirrhosis (PBC). METHODS: CpnIq/G and IgM were determined by enzyme-linked immunosorbent assay (ELBA) in 41 well-established PBC patients and two race-matched control groups (post-hepatitis cirrhosis, n = 70; healthy controls, n = 57). RESULTS: The mean level and seroprevalence of Cpn IgG in PBC group and post-hepatitis cirrhosis (PHC) group were significantly higher than those in healthy controls (46.8±43.4 RU/mL, 49.5±45.2 RU/mL vs28.3±32.7 RU/mL; 68.3%, 71.4%, 42.1%, respectively; P<0.05). There was a remarkably elevated seroprevalence of Cpn IgM in patients with PBC (22.0%) compared to the PHC and healthy control (HC) groups. For the PBC patients versus the HCs, the odds ratios (ORs) of the presence of Cpn IgG and IgM were 2.7 (95% CI 0.9-6.1) and 5.1 (95% CI 1.4-18.5), respectively. Though there was no correlation in the level of Cpn IgG with total IgG in sera of patients with PBC (r = -0.857, P = 0.344>0.05), Cpn IgM was related with the abnormally high concentrations of total IgM in PBC group. CONCLUSION: The results of this study do not support the hypothesis that infection with Chlamydia pneumoniae may be a triggering agent or even a causative agent in PBC, but suggest that Chlamydia pneumoniae infection probably contributes to the high level of IgM present in most patients with PBC.

Inhibition of Urogenital Chlamydia Trachomatis in Vitro by 12 Diuretic Traditional Chinese Medicines

Objective: To detect the inhibition of urogenitalchlamydia trachomatis (CT) by 12 traditional Chinesemedicines in vitro.Methods: The inhibition of CT isolates by these medicineswas detected by micro-culture technique with McCoy cellsin vitro. Results: All the diuretic traditional Chinese medicinesinhibited urogenital CT The minimum inhibitoryconcentrations (MICs) ranged from 0.122 mg ml^(-1) to 62.5mg ml^(-1). Diathus superbus L., Poria cocos (Shew.) Woft,Polyporus umbellatus (Pers.) Fries, and Artemisia capillariesThunb showed stronger inhibition than the other eighttraditional Chinese medicines. The numbers and sizes ofinclusions bodies reduced gradually and disappeared finallywith the increase of the concentrations. Conclusion: All the 12 diuretic traditional Chinesemedicines inhibited urogenital CT.

Progress of Study on Chlamydial Pathogenesis

Chlamydia, a human pathogen that mediates chronic, persistent and repeated infection progress, is capable of inflicting permanent scar formation. A typical reticular body is found in persistent infections. Chlamydial Hsp 60, interferon(IFN) -gamma and other cytokines function in the course of repeated infection. Immunopathological factors mediate chronic infection.

Advances on Syndromic Management of Sexually Transmitted Diseases

Research data showed that syndromic approach could successfully manage gonococcal and chlamydial infections in males and syphilis and chancroid in males and females. However, low sensitivity, specificity and positive predictive value were found in the syndromic management of vaginal discharge. It is recommended that the syndromic algorithm for management of vaginal discharge used when serving high-risk and symptomatic women.

Derivatives of Thiohydrazides as Effective Antibacterial Remedies for Chlamydial Infection Treatment at Chronic Stages of Infections

Hydrazones of poorly studied fluorine-containing oxamic acid thiohydrazides were synthesized by the reaction with salicylaldehydes. Tests showed that the newly synthesized compounds were effective low-toxic inhibitors of type III secretion system in Chlamydia trachomatis.

Detection of Genitourinary Tract Chlamydia trachomatis Infection in Urine specimens by PCR Assay

Objective: To compare the sensitivity and specificity of thecervical/urethral swabs with voided urine specimens for thedetection of genitourinary tract infection with Chlamydia tra-chomatis and determine whether urine specimens can replacethe cervical/urethral swabs in detection of C. thachomatis. Methods: The matched cervical/urethral swabs and voidedurine specimens were collected from 569 patients of STD clinics.Polymerase chain reaction (PCR) assay specific for C. tra-chomatis plasmid DNA and rapid antigen testing (Clear viewassay) was used to detect C trachomatis. Standard criteria thatdefined 'true' positive included: 1) positive PCR results both incervical/urethral swab and voided urine specimen or 2) positivevoided urine results both by PCR assay and clear view test or 3)positive results in both PCR assay of cervical/urethral swab andclear view test of voided urine. For statistical analysis, thechi-square test was used. Results: The prevalence of C trachomatis in patients withsymptoms was 12.1% (28/231) in women and 10.4%(10/96) inmen, with no significant difference between them (x^2=0.21,P>0.05). The prevalence of C trachomatis in patients with nosymptoms was 11.0% (11/100) in women and 15.5% (22/142) inmen, with a significant difference existing between them. (x^2=4.0, P<0.05). No significant difference (P>0.05) existed betweenPCR testing of swabs (sensitivity 87.3%; specificity 99.2%) andPCR testing of urine (sensitivity 88.7%: specificity 98.8%). Asfor clear view assay, sensitivity was 60.6% and specificity was100%. Conclusions: PCR assay is superior to clear view in detectingC trachomatis. Although both PCR testing of swabs and PCRtesting of urine specimens both have high sensitivity and speci-ficity, urine specimen testing is more cost-effective, practical andnoninvasive. Thus urine specimens can take the place of theswabs in PCR testing for chlamydia.

Clinical Study of Continuous Infection in the Urogenital System of Chlamydiae trachomatis

Objective： To analyze the causes of persistent infection of Chlamydia trachomatis in the urogenital system. Method： We followed 223 patients with Chlamydia trachomatis infection who were treated regularly. Result： After treatment, 22.87% of cases still tested positive. After one year and change of treatment regime,4.48% of cases remained positive, most of whom were female. Conclusion： The course of Chlamydia trachomatis infection in the urogenital system is varied. This diversity has many causes including immunocompetence the characteristics of chalmydia trchomatis infection and genetic resistance.

Measurement of Antibodies and Cytokines in Chlamydia Trachomatis-related Infertile Patients

Objectives: To find out the level and functions of Chlamydia trachomatis heat shock protein (C-hsp60) antibody, anti-spermantibody(ASAb), interleukin 1(IL-1), interleukin 6 (IL-6),interleukin 8 (IL-8), Tumor necrosis factor alpha (TNF-α)and γ-interferon (IFN-γ) in patients with CT-related infertility. Methods: CT-DNA of cervical secretions was detectedthrough polymerase chain reaction (PCR) and migrationinhibiting factor (MIF) was employed to measure IgG titre ofCT MOMP antibody. Western blot was used to determinepresence of C-hsp60 antibody and enzyme-linkedimmunoadsorbent assay (ELISA) measured ASAb of IgG typein blood serum and determine the content of IL-1, IL-6. IL-8.TNF-α. IFN-γ in uterine tube fluid. Results: 68 patients had positive CT-DNA, among which 57(83.8%) had C-hsp60 antibody. Among the 172 patients withnegative CT-DNA, 64 patients (37.2%) also had C-hsp60Antibody. There was a significant difference (P<0.01) betweeninfertile patients and control group patients in the presence ofAAb. Infertile patients with positive CT-DNA had higher levels of IL-1、IL-6. IL-8. TNF-α. IFN-γ in uterine tube fluidcompared to control group patients (P<0.01). Conclusion: Firstly, those patients with negative CT testingfrom cervical secretions cannot be ruled out for CT infectionin deep parts of the body (such as oviduct, pelvic kidney).Detection of C-hsp60 Antibody may help to diagnose suchcases of CT. Secondly, CT infection of the oviduct can raiselevels of IL-1、IL-6、 IL-8、 TNF-α. IFN-γ. The pathogenesis ofinfertility caused by CT infection in the reproductive tractmay be related to cytokine production and inflammatoryresponses mediated by C-hsp60 Antibody, IL-1, IL-6, IL-8,TNF-α, and IFN-γ.

Establishment of a Two-site ELISA for Detection of Chlamydia Trachomatis

Objective:To establish a rapid and simple assay for the diagnosis of Chlamydia trachomatis (CT) infection. Methods BALB/c mice were immunized with SDS-PAGE purified major outer membrane protein (MOMP) from CT and the monoclonal antibodies were obtained subsequently. Two-site ELISA was developed to detect CT infection. Results: The established assay was able to detect as low as 1.248ug/ml MOMP with interrun and inrun CV 6.9% and 3.1% respectively. 94% (34/36) of culture-positive samples were found to be positive in the current examination, indicating the high sensitivity of this assay. Conclusion: The assay is applicable for clinical diagnosis of CT infection.

manClinical Study on Infection of Chlamydia Trachomatis in Patients with Inflammation of Urogenital Tract

Object: To investigate the relationship between chlamydiatrachomatis (CT) and urogenital infection. Method Positive rate of CT in patients with inflammationof urogenital tract was significantly higher than those withoutinflammation(P<0.05). Result: There was statistical difference in the males nomatter they were patients with inflammation of urogenitaltract or not (P>H0.05), while there was no statistical differencein females (P>0.05). The incidence of the infection was highamong those aging from 21-50 years old. Conclusion: The clinical manifestations of CT infectionwere obscure, so we should examine CT in patients who haveno symptoms, especially in females and those of high-riskpopulation.

Indirect Enzyme-Linked Immunosorbent Assay Based on the Nucleocapsid Protein of SARS-like Coronaviruses

The nucleocapsid protein(N) is a major structural protein of coronaviruses. The N protein of bat SARS-like coronavirus(SL-CoV) has a high similarity with that of SARS-CoV. In this study,the SL-CoV N protein was expressed in Escherichia coli,purified and used as antigen. An Indirect Enzyme-Linked Immunosorbent Assay(indirect ELISA) was developed for detection of SARS-or SL-CoV infections in bat populations. The detection of 573 bat sera with this indirect ELISA demonstrated that SL-CoVs consistently circulate in Rhinilophus species,further supporting the proposal that bats are natural reservoirs of SL-CoVs. This method uses 1-2 μl of serum sample and can be used for preliminary screening of infections by SARS-or SL-CoV with a small amount of serum sample.

Vidas CHL Assay in the Detection of Urogenital Chlamydia Trachomatis Infection

Objectives: To evaluate the Vidas Chlamydia (CHL) assayfor detecting C.Trachomatis with swabs and first catch urine(FCU) specimens from STD patients and high riskpopulations. Methods: A total of 383 pahents were tested with tissueculture (TC), Vidas CHL and polymerase chain reaction (PCR)for C.trachomatis on male and female swabs, with Vidas CHLtesting male FCU specimens. CHL positive and equivocalresults were confirmed with a blocking assay (CHB). Truepositive were defined as either TC positive, or TC negtive butCHL and PCR positive. The performance of TC, CHL andPCR were evaluated according to this expanded goldstandard. Results: Compared with the expanded gold standard, 54 ofthe 232 male specimens were true positive results. For maleswabs, TC, CHL and PCR had sensitivities of 90.7%, 96.3%and 94.4%, and specificities of 100%, 98.3% and 97.2%,respectively. Differences were not statistically significant. Formale FCU specimens, CHL sensitivity and specificity were83.3% and 98.3%; there was little difference between theseresults and that of matched swabs. Compared with theexpanded gold standard, 28 of the 151 female swabs were truepositive; TC, CHL and PCR had sensitivities of 82.1%, 100%and 96.4%, and specificities of 100%, 98.4% and 97.6%,respectively. The difference was also not significant. Conclusions: Vidas CHL assay is very scnsitive and specificfor C.trachomatis detection with swab specimens of male andfemale STD patients. For male FCU specimens, the assay alsohad high sensitivity and specificity. CHB may not be needed inthe routine detection or Chlamydia infections. Populationswith higher incidence of C.trachomatis infection.