AIM： To assess each layer of the optical coherence tomography （OCT） image of the esophageal wall with reference to the histological structure, METHODS： Resected specimens of fresh pig esophagus was used as a model...AIM： To assess each layer of the optical coherence tomography （OCT） image of the esophageal wall with reference to the histological structure, METHODS： Resected specimens of fresh pig esophagus was used as a model for the esophageal wall. We injected cyanoacrylate adhesive into the specimens to create a marker, and scanned them using a miniature OCT probe. The localization of these markers was assessed in the OCT images. Then we compared the OCT-imaged morphology with the corresponding histological section, guided by the cyanoacrylate adhesive markers. We prepared a second set of experiments using nylon sutures as markers. RESULTS： The OCT image of the esophageal specimen has a clear five-layered morphology. First, it consisted of a relatively less reflective layer; second, a more reflective layer; third, a less reflective layer; fourth, a more reflective layer; and fifth, a less reflective layer. Comparing the OCT images with marked histological sections showed that the first layer corresponded to stratified squamous epithelium; the second to lamina propria; the third to muscularis mucosa; fourth, submucosa; and fifth, muscularis propria with deeper structures of the esophageal wa CONCLUSION： We demonstrated that the OCT image of the normal esophageal wall showed a five- layered morphology, which corresponds to histological esophageal wall components.展开更多
Two new photolabile nucleotide analogues with furan-fused deoxyuridine were synthesized through Sonogashira coupling.Their enzymatic incorporation into DNA was evaluated with two DNA polymerases(Taq and Deep vent exo-...Two new photolabile nucleotide analogues with furan-fused deoxyuridine were synthesized through Sonogashira coupling.Their enzymatic incorporation into DNA was evaluated with two DNA polymerases(Taq and Deep vent exo-)by polymerase chain reaction(PCR).Deep vent exo-recognized both nucleotides as substrates for primer extension,while Taq was much less proficient.Light irradiation of PCR products released the amino and carboxyl moieties of DNA.Further labeling with fluorescein isothiocyanate for a long DNA construct with F-dUnTP incorporation was successfully achieved.展开更多
文摘AIM： To assess each layer of the optical coherence tomography （OCT） image of the esophageal wall with reference to the histological structure, METHODS： Resected specimens of fresh pig esophagus was used as a model for the esophageal wall. We injected cyanoacrylate adhesive into the specimens to create a marker, and scanned them using a miniature OCT probe. The localization of these markers was assessed in the OCT images. Then we compared the OCT-imaged morphology with the corresponding histological section, guided by the cyanoacrylate adhesive markers. We prepared a second set of experiments using nylon sutures as markers. RESULTS： The OCT image of the esophageal specimen has a clear five-layered morphology. First, it consisted of a relatively less reflective layer; second, a more reflective layer; third, a less reflective layer; fourth, a more reflective layer; and fifth, a less reflective layer. Comparing the OCT images with marked histological sections showed that the first layer corresponded to stratified squamous epithelium; the second to lamina propria; the third to muscularis mucosa; fourth, submucosa; and fifth, muscularis propria with deeper structures of the esophageal wa CONCLUSION： We demonstrated that the OCT image of the normal esophageal wall showed a five- layered morphology, which corresponds to histological esophageal wall components.
基金supported by the National Natural Science Foundation of China(21072015)the National Basic Research Program of China(973 Program,2012CB720600)+1 种基金the Program for New Century Excellent Talents in University(NCET-10-0203)the State Key Laboratory of Drug Research(SIMM1106KF-15)
文摘Two new photolabile nucleotide analogues with furan-fused deoxyuridine were synthesized through Sonogashira coupling.Their enzymatic incorporation into DNA was evaluated with two DNA polymerases(Taq and Deep vent exo-)by polymerase chain reaction(PCR).Deep vent exo-recognized both nucleotides as substrates for primer extension,while Taq was much less proficient.Light irradiation of PCR products released the amino and carboxyl moieties of DNA.Further labeling with fluorescein isothiocyanate for a long DNA construct with F-dUnTP incorporation was successfully achieved.
