Screening study on new tumor marker periplakin for lung cancer

Objective: The aim of this study was to use lung cancer targeting binding polypeptide ZS-9 to screen cDNA library of human lung cancer and obtain ZS-9 specific ligand to confirm tumor marker of non small-cell lung cancer. Methods: Artificially synthesize biotin labeled peptide ZS-9, anchored ZS-9 in the enzyme label plate coupled by avidin, used ZS-9 as probe to screen cDNA library of human lung cancer, after screening, obtained bacteriophage clone specifically binding with anchored polypeptide ZS-9. Extracted plasmid of bacteriophage and performed sequencing after amplified by PCR. Results: It was demonstrated by bioinformatic analysis on the sequence of ligand binded by lung cancer specific peptide ZS-9 that the ligand was the cytoskeletal protein periplakin on the surface of lung cancer cells, suggesting that periplakin might be a new marker for non-small-cell lung cancer in lung cancer. Conclusion: Use specific lung cancer binding peptide to screen new tumor marker periplakin in lung cancer and further studies on its biologic functions in genesis and development of lung cancer are still needed.

西安博物馆设计

最近落成的西安博物馆是西安博物院当中的主馆。该博物院是在古都西安以小雁塔为标志的寺庙建筑群、博物馆、公园三位一体的现代大型博物院。博物馆设计力求在和古迹和谐统一的环境中创造自身特色,在城市中具有标志性,与公园互相成景、得景。设计师们一如既往追求传统的建筑理念与现代功能相结合,传统的审美意识与现代审美意识相结合,传统的造型规律与现代建筑的技术手段、手法相结合。