In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its nea...In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its near-isogenic line TIST2 carrying S-b(i)/S-b(i) were used to develop the mapping population. One hundred and fifty-eight microsatellite markers were selected to survey T65 and TISL2. RM13 on chromosome 5 was found to be polymorphic between them. Cosegregation indicated that RM13 was closely linked with locus S-b. Eleven RFLP markers were selected on the corresponding region from the genetic map of Rice Genome Research Program (RGP) of Japan to convert into sequence-tagged site (STS) markers. Amplicon length polymorphism (ALP) was carried out, but none of them was found to be polymorphic between T65 and TISL2. Then PCR-based RFLP (PBR) was done using six 4-nucleotide recognizing restriction endonucleases. Polymorphism was detected when PCR products of R830STS and R2213SSTS were digested with Taq I. Genetic analysis indicated that the distance between locus S-b and markers, R830STS, RM13 and R2213SSTS were 3.3 cM (centi-Morgan), 5.2 cM and 5.5 cM, respectively. These PCR-based markers could be directly used in marker-assisted selection. The technical system combining genetic mapping and PCR-based marker-assisted selection will facilitate the development of molecular breeding.展开更多
[Objective] This study aimed to analyze the quantitative trait loci （QTLs） of grain storage durability for maize under controlled deterioration conditions by using SSR markers. [Method] The recombinant inbred line p...[Objective] This study aimed to analyze the quantitative trait loci （QTLs） of grain storage durability for maize under controlled deterioration conditions by using SSR markers. [Method] The recombinant inbred line population F 2 derived from the cross of shen 137/02-50 was used to do the simple sequence repeats （SSRs） and QTL analysis. [Result] The 2 parents showed good polymorphisms. Three loci were detected on chromosome 1, chromosome 6 and chromosome 9, accounting for 41.2% of total phenotypic. [Conclusion] This study provided theoretical basis for using molecular marker to assist the breeding of storage durable maize varieties.展开更多
With joint analysis based on the parents, F 1, F 2 and backcrosses, the authors found that the resistance of the maize inbred line Huangzaosi to the maize dwarf mosaic virus strain B was conditioned by a major gene ...With joint analysis based on the parents, F 1, F 2 and backcrosses, the authors found that the resistance of the maize inbred line Huangzaosi to the maize dwarf mosaic virus strain B was conditioned by a major gene and polygene, and identified a new major gene. Bulked segregate and microsatellite analysis of a F 2 progeny from the combination of Huangzaosi×Mo17 were used to identify the resistance gene, mdm1(t), on the long arm of chromosome 6. This new resistance gene is tightly linked to and located between the microsatellite markers loci, phi077 and bnlg391. The linkage distances between phi077-mdm1(t) and mdm1(t)-bnlg391 are 4.74 centiMorgan (cM) and 6.72 cM respectively.展开更多
Genetic comparison between Rhynchophorus ferrugineus （Coleoptera： Curculionidae： Rhynchophorinae） and other four Rhynchophorus spp. such as R. bilineatus, R. phoenicis, R. cruentatus and R. palmarum was investigat...Genetic comparison between Rhynchophorus ferrugineus （Coleoptera： Curculionidae： Rhynchophorinae） and other four Rhynchophorus spp. such as R. bilineatus, R. phoenicis, R. cruentatus and R. palmarum was investigated using partial sequences of Cytochrome c oxidase subunit I （COl） mitochondrial gene. The number ofpolymorphic sites ranged from 79 to 102 while the genetic distances ranged from 0.058 to 0.095. The number of polymorphic sites, genetic distances and phylogenetic relationships among R. ferrugineus and the other four tested species revealed that R.ferrugineus was more closely related to R. bilineatus while it was distantly related to R. palmarum. It is possible to rank the five tested species as follows： R.ferrugineus, R. bilineatus, R. phoenicis, R. cruentatus and R. palmarum. The result of this study is the first step of the revision of the uncertain taxonomic status and phylogenetic relationships among the Rhynchophorus genus. Further analysis should be performed using other Rhynchophorus species as well as other molecular markers.展开更多
Net blotch disease caused by the fungus Pyrenophora teres belongs to the complex of leaf blotch diseases that decrease the yield and the quality of barley （Hordeum vulgate L.）. This study deals with the use of micro...Net blotch disease caused by the fungus Pyrenophora teres belongs to the complex of leaf blotch diseases that decrease the yield and the quality of barley （Hordeum vulgate L.）. This study deals with the use of microsatellites localized nearby the quantitative trait loci, associated with the resistance to P. teres to screen barley lines and varieties that could be introduced into a net blotch resistance breeding program. Thirty-five barley microsatellite loci and 65 barley genotypes, 265 alleles were detected. The number of alleles per locus ranged from 2 to 26. The arrangement of genotypes into clusters based on microsatellite data does not correspond to their resistance level to P. teres even though chosen microsatellite markers have been localized nearby QTLs associated with the resistance to P. teres.展开更多
In view of the movable hook structure on zero position,the traditional system could not recognize the zero scale.According to the essential requirements of the measurement,a new system based on machine vision was prop...In view of the movable hook structure on zero position,the traditional system could not recognize the zero scale.According to the essential requirements of the measurement,a new system based on machine vision was proposed.Standard datum planes and standard scales were designed as standard component,and the indication error can be calculated by comparing the standard component and the measured value between zero position and 500 mm scale.The alignment of scribed lines was realized by machine vision,and the float pixel equivalent method was applied in image process.Experimental results prove that this system meets the requirement of national standard steel tape verification regulation,and the repeatability of zero error can reach 0.006 mm after zero calibration.展开更多
Five molecular related indexes: MOL, SIM, d2, H0and PIC of 15 turbot parent pairs were estimated by using 10 SSR loci, which were used in correlation analysis with growth traits, DIL and DIW, of family filial from tho...Five molecular related indexes: MOL, SIM, d2, H0and PIC of 15 turbot parent pairs were estimated by using 10 SSR loci, which were used in correlation analysis with growth traits, DIL and DIW, of family filial from those 15 parent pairs.DIL and DIW were regressed on the MOL, SIM, d2, H0and PIC. Results showed MOL of five SSR loci(12, 17, 24, 81 and 85) and SIM of five loci(17, 21, 24, 81and 85) all shared significant positive correlation with DIL(r=0.482, P=0.035 and r=0.479, P=0.035, respectively); H0of six SSR loci(11, 17, 21, 24, 26 and 85) had significant positive correlation with DIW(r=0.551, P=0.017); PIC of two SSR loci(9and 26) had significant positive(r=0.519, P=0.024) correlation with DIL, while that and of four loci(17, 24, 27 and 85) had significant negative correlation(r=-0.519,P=0.024), with DIL. This present study suggested that filial growth expression could be predicted by using molecular related indexes in turbot breeding practice, and the accuracy of prediction depends on more SSR loci, especially associated with QTL.展开更多
Dear Editor,Although simple sequence repeat（SSR）markers are not new,they are still useful and often used markers in molecular mapping and marker-assisted breeding,particularly in developing countries.However,locus-s...Dear Editor,Although simple sequence repeat（SSR）markers are not new,they are still useful and often used markers in molecular mapping and marker-assisted breeding,particularly in developing countries.However,locus-specific SSR markers could be more useful and informative in wheat breeding and genetic studies.In the present study,221,911 locus-specific SSR markers were designed.Verification of polymorphisms showed that the proportion of polymorphic markers increases with an increase in SSR size.展开更多
文摘In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its near-isogenic line TIST2 carrying S-b(i)/S-b(i) were used to develop the mapping population. One hundred and fifty-eight microsatellite markers were selected to survey T65 and TISL2. RM13 on chromosome 5 was found to be polymorphic between them. Cosegregation indicated that RM13 was closely linked with locus S-b. Eleven RFLP markers were selected on the corresponding region from the genetic map of Rice Genome Research Program (RGP) of Japan to convert into sequence-tagged site (STS) markers. Amplicon length polymorphism (ALP) was carried out, but none of them was found to be polymorphic between T65 and TISL2. Then PCR-based RFLP (PBR) was done using six 4-nucleotide recognizing restriction endonucleases. Polymorphism was detected when PCR products of R830STS and R2213SSTS were digested with Taq I. Genetic analysis indicated that the distance between locus S-b and markers, R830STS, RM13 and R2213SSTS were 3.3 cM (centi-Morgan), 5.2 cM and 5.5 cM, respectively. These PCR-based markers could be directly used in marker-assisted selection. The technical system combining genetic mapping and PCR-based marker-assisted selection will facilitate the development of molecular breeding.
基金Supported by the National Science Fund for Young Scholars of China (31101598)the Natural Science Fund of Anhui Province, China (090411002)+2 种基金the Youth Science Fund of Anhui Province, China (10040606Y02)the Innovation Team of Corn of Anhui Science and Technology University (2011AKKC2011-1)the Key Disciplines of Anhui Science and Technology University (AKXK2010-1-1)~~
文摘[Objective] This study aimed to analyze the quantitative trait loci （QTLs） of grain storage durability for maize under controlled deterioration conditions by using SSR markers. [Method] The recombinant inbred line population F 2 derived from the cross of shen 137/02-50 was used to do the simple sequence repeats （SSRs） and QTL analysis. [Result] The 2 parents showed good polymorphisms. Three loci were detected on chromosome 1, chromosome 6 and chromosome 9, accounting for 41.2% of total phenotypic. [Conclusion] This study provided theoretical basis for using molecular marker to assist the breeding of storage durable maize varieties.
文摘With joint analysis based on the parents, F 1, F 2 and backcrosses, the authors found that the resistance of the maize inbred line Huangzaosi to the maize dwarf mosaic virus strain B was conditioned by a major gene and polygene, and identified a new major gene. Bulked segregate and microsatellite analysis of a F 2 progeny from the combination of Huangzaosi×Mo17 were used to identify the resistance gene, mdm1(t), on the long arm of chromosome 6. This new resistance gene is tightly linked to and located between the microsatellite markers loci, phi077 and bnlg391. The linkage distances between phi077-mdm1(t) and mdm1(t)-bnlg391 are 4.74 centiMorgan (cM) and 6.72 cM respectively.
文摘Genetic comparison between Rhynchophorus ferrugineus （Coleoptera： Curculionidae： Rhynchophorinae） and other four Rhynchophorus spp. such as R. bilineatus, R. phoenicis, R. cruentatus and R. palmarum was investigated using partial sequences of Cytochrome c oxidase subunit I （COl） mitochondrial gene. The number ofpolymorphic sites ranged from 79 to 102 while the genetic distances ranged from 0.058 to 0.095. The number of polymorphic sites, genetic distances and phylogenetic relationships among R. ferrugineus and the other four tested species revealed that R.ferrugineus was more closely related to R. bilineatus while it was distantly related to R. palmarum. It is possible to rank the five tested species as follows： R.ferrugineus, R. bilineatus, R. phoenicis, R. cruentatus and R. palmarum. The result of this study is the first step of the revision of the uncertain taxonomic status and phylogenetic relationships among the Rhynchophorus genus. Further analysis should be performed using other Rhynchophorus species as well as other molecular markers.
文摘Net blotch disease caused by the fungus Pyrenophora teres belongs to the complex of leaf blotch diseases that decrease the yield and the quality of barley （Hordeum vulgate L.）. This study deals with the use of microsatellites localized nearby the quantitative trait loci, associated with the resistance to P. teres to screen barley lines and varieties that could be introduced into a net blotch resistance breeding program. Thirty-five barley microsatellite loci and 65 barley genotypes, 265 alleles were detected. The number of alleles per locus ranged from 2 to 26. The arrangement of genotypes into clusters based on microsatellite data does not correspond to their resistance level to P. teres even though chosen microsatellite markers have been localized nearby QTLs associated with the resistance to P. teres.
文摘In view of the movable hook structure on zero position,the traditional system could not recognize the zero scale.According to the essential requirements of the measurement,a new system based on machine vision was proposed.Standard datum planes and standard scales were designed as standard component,and the indication error can be calculated by comparing the standard component and the measured value between zero position and 500 mm scale.The alignment of scribed lines was realized by machine vision,and the float pixel equivalent method was applied in image process.Experimental results prove that this system meets the requirement of national standard steel tape verification regulation,and the repeatability of zero error can reach 0.006 mm after zero calibration.
文摘Five molecular related indexes: MOL, SIM, d2, H0and PIC of 15 turbot parent pairs were estimated by using 10 SSR loci, which were used in correlation analysis with growth traits, DIL and DIW, of family filial from those 15 parent pairs.DIL and DIW were regressed on the MOL, SIM, d2, H0and PIC. Results showed MOL of five SSR loci(12, 17, 24, 81 and 85) and SIM of five loci(17, 21, 24, 81and 85) all shared significant positive correlation with DIL(r=0.482, P=0.035 and r=0.479, P=0.035, respectively); H0of six SSR loci(11, 17, 21, 24, 26 and 85) had significant positive correlation with DIW(r=0.551, P=0.017); PIC of two SSR loci(9and 26) had significant positive(r=0.519, P=0.024) correlation with DIL, while that and of four loci(17, 24, 27 and 85) had significant negative correlation(r=-0.519,P=0.024), with DIL. This present study suggested that filial growth expression could be predicted by using molecular related indexes in turbot breeding practice, and the accuracy of prediction depends on more SSR loci, especially associated with QTL.
基金supported by the National Natural Science Foundation of China(31270704,31600997)the Yangzhou Key Research and Development Program(YZ2016035)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘Dear Editor,Although simple sequence repeat（SSR）markers are not new,they are still useful and often used markers in molecular mapping and marker-assisted breeding,particularly in developing countries.However,locus-specific SSR markers could be more useful and informative in wheat breeding and genetic studies.In the present study,221,911 locus-specific SSR markers were designed.Verification of polymorphisms showed that the proportion of polymorphic markers increases with an increase in SSR size.
