气相OH标准发生源的建立和研究

利用H2 O在 184 9nm紫外光照射下光解产生OH自由基建立了气相OH标准发生源 ,通过测量气流中O3和H2 O浓度计算得到OH浓度 ,此计算浓度与利用水杨酸液相吸收 高效液相色谱法测得的OH浓度比较一致 ,同时用激光诱导荧光技术对可能存在的壁效应进行研究 ,结果表明在较高流速下其影响较小。

气相-内标法测定白兰地中甲醇、乙酸乙酯、糠醛和高级醇

建立了气相-内标标准曲线法同时测定白兰地中甲醇、乙酸乙酯、糠醛、高级醇（正丙醇＋异戊醇＋异丁醇＋正丁醇＋仲丁醇）含量的方法,样品添加内标后直接进样,经CP-Wax 57毛细管色谱柱分离后用FID检测器进行分析。在所建立的最佳试验条件下,白兰地中各组分峰得到有效分离,标准曲线相关系数均在0.999 60以上,回收率在94.9%~117.7%之间。该方法灵敏、易操作、准确可靠,能够对白兰地中甲醇、乙酸乙酯、糠醛、高级醇进行准确的定性定量分析。

气相色谱内标法测定注射级聚山梨酯80中乙二醇、二甘醇、三甘醇残留量

目的:建立注射级聚山梨酯80中乙二醇、二甘醇、三甘醇残留溶剂检测方法。方法:采用毛细管气相色谱法,以6%氰丙基苯基-94%二甲基聚硅氧烷(DB-624,60 m×0.32 mm×1.8μm)为固定相,柱温为程序升温:初始温度为60℃,以每分钟10℃的速率升温至110℃,维持5 min,再以每分钟40℃的速率升温至170℃,维持5 min;再以每分钟40℃的速率升温至230℃,维持10 min。进样口温度为230℃,检测器温度为250℃;载气(N)流速为5 mL/min。结果:在确定的色谱条件下分离度良好;3种溶剂检测限均满足验证要求,具有良好的线性关系,相关系数(r)均大于0.9990;重复性试验测定结果的RSD(n=6)为2.1%~3.2%,中间精密度试验各溶剂测定结果的RSD(n=12)为1.9%~2.6%;各溶剂回收率为97.2%~100.0%,在80%~120%范围内,且各溶剂结果RSD(n=9)不超过10%。结论:建立的方法专属性强、灵敏度高、有良好的线性关系、精密度和准确度高,可为注射级聚山梨酯80残留溶剂的质量控制提供参考。

Computational Simulation of Aptamer-target Binding Mechanisms

Aptamers are a type of single-chain oligonucleotide that can combine with a specific target.Due to their simple preparation,easy modification,stable structure and reusability,aptamers have been widely applied as biochemical sensors for medicine,food safety and environmental monitoring.However,there is little research on aptamer-target binding mechanisms,which limits their application and development.Computational simulation has gained much attention for revealing aptamer-target binding mechanisms at the atomic level.This work summarizes the main simulation methods used in the mechanistic analysis of aptamer-target complexes,the characteristics of binding between aptamers and different targets(metal ions,small organic molecules,biomacromolecules,cells,bacteria and viruses),the types of aptamer-target interactions and the factors influencing their strength.It provides a reference for further use of simulations in understanding aptamer-target binding mechanisms.

气相色谱内标法测定牡荆油胶丸中β-丁香烯含量的不确定度评定

目的:建立气相色谱法(GC)测定牡荆油胶丸中β-丁香烯含量的测量不确定度评定方法。方法:通过建立GC内标法测定含量的数学模型,对各个不确定度因素进行评估、计算,由此计算合成不确定度,最终给出测量结果在95%置信区间下的扩展不确定度。结果:本次实验的牡荆油胶丸β-丁香烯含量测定结果为(84.25±3.59)mg.g-1。结论:建立的不确定度评定法适用于气相内标法测定药物含量的不确定度分析。

RFLP Detection of Genetic Variation of Maize Inbred Lines

Genetic similarities of 13 inbred lines of maize (Zea mays L.) were analyzed by restriction fragment length polymorphisms (RFLPs). The objectives of the study were to detect genetic similarities among 13 inbreds and to assign them to heterotic groups. By means of 24 probe_enzyme combinations (PECs) selected for locus specificity, clear patterns and reproducibility, 85 alleles were found with an average of 3.3 alleles per locus. The allelic frequency data were used to estimate genetic similarities among lines, and as a result the diversity index of 0.499 was obtained. Genetic similarities between the pairs of 13 lines ranged from 0.523 up to 0.802 with an average of 0.649. The UPGMA clustering algorithm analysis classified the 13 lines into five groups, which generally corresponded to known maize heterotic groups based on pedigree information. The authors concluded that RFLP_based markers could be used for investigating genetic relationships between maize inbred lines and assigning them to heterotic groups, but it seemed that a large number of PECs were needed to obtain reliable estimates of genetic similarity.

Icon memory research under different time pressures and icon quantities based on event-related potential

In order to obtain related brain electrical components and neural bases of physiology assessment of icon elements in a digital human-computer interface the modified sample-delay matching task experimental paradigm is used under different time pressures 4 000 and 2 000 ms and different icon quantities three five and ten icons on icon memory based on event-related potential ERP technology.Experimental results demonstrate that P300 has significant volatility changes and the maximum amplitude around the middle line of the parietal area PZ and P200 has obvious volatility changes around the middle line of the frontal and central area FCZ during icon cognition.P300 and P200 amplitudes increase as tasks become more difficult.Thus P300 latency is positively correlated with task difficulty. ERP research on the characteristics of icon memory will be an important reference standard in guiding user neurocognitive behavior and physiology assessment on interface usability.

Combined Method of Datum Transformation Between Different Coordinate Systems

The similarity transformation model between different coordinate systems is not accurate enough to describe the discrepancy of them.Therefore,the coordinate transformation from the coordinate frame with poor accuracy to that with high accuracy cannot guarantee a high precision of transformation.In this paper,a combined method of similarity transformation and regressive approximating is presented.The local error accumulation and distortion are taken into consideration and the precision of coordinate system is improved by using the recommended method

Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique

Powdery mildew is a serious disease of wheat in China. As part of ITEC (International Triteace EST cooperation), EST (expressed sequence tags) technique was used to explore the gene expression in leaf induced by Erysiphe graminis DC. A conventional cDNA library was constructed, and a total of 1 500 clones picked randomly from the library were sequenced, three hundred and eighty_seven ESTs of them were unique, which got the Accession Number in GenBank. About 49.4% ESTs showed significant similarity to functions of known sequences in GenBank. There are 196 ESTs' with functions not able to be determined, and eighty_four ESTs were demonstrated to be novel sequences. High_density dot membranes from unique clones were produced, and several disease resistance related genes were screened by differential hybridization.

APPROACH FOR FUZZY MULTI-ATTRIBUTE DECISION-MAKING WITH FUZZY COMPLEMENTARY PREFERENCE RELATION ON ALTERNATIVES

In presented fuzzy multi-attribute decision-making （FMADM） problems, the information about attribute weights is interval numbers and the decision maker （DM） has fuzzy complementary preference relation on alternatives. Firstly, the decision-making information based on the subjective preference information in the form of the fuzzy complementary judgment matrix is uniform by using a translation function. Then an objective programming model is established. Attribute weights are obtained by solving the model, thus the fuzzy overall values of alternatives are derived by using the additive weighting method. Secondly, the ranking approach of alternatives is proposed based on the degree of similarity between the fuzzy positive ideal solution of alternatives （FPISA） and the fuzzy overall values. The method can sufficiently utilize the objective information of alternatives and meet the subjective requirements of the DM as much as possible. It is easy to be operated and implemented on a computer. Finally, the proposed method is applied to the project evaluation in the venture investment.

Winter Wheat Salt Tolerance Appraisal Indices with Varied Mineral Content of Saline Water

[Objective] The aim was to study wheat salt resistance appraisal indicators under the condition of saline water irrigation.[Method] A trial was conducted with five varieties irrigated with saline water at 1,2,4,6,and 8 g/L during 2009-2010 and 2010-2011 in the research station of Dry-land Farming Institute of Hebei Academy of Agricultural and Forestry Sciences.With standardized indicators for measuring treatment,the response relations among different salt stress levels and winter wheat growth index were analyzed in this study.[Result] Under the condition of different salinity of irrigation,relative plant height after jointing stage,relative leaf area index,relative dry matter weight,relative ear number per unit area showed significant differences among treatments and yield showed significant correlation,which can be taken as salt-resistance examination index of wheat.[Conclusion] The results showed that the relative height after jointing stage was recommended as the most practical salt-tolerance appraisal indices,because it was easy to be observed and sensitive to salt stress.

Design of beacon system based on MSP430 micro- controller

In order to solve the onboard recorder search problem, a new beacon system based on radio direction finding tech- nique, which indicates the orientation of the recorder, is designed. The system consists of phase-locked loop （PLL）, micro- processor, global position system （GPS） module, power module and so on. The scene simulation verifies the feasibility and reliability of the system. This beacon system can be applied to the recorder search in many fields, and can effectively reduce the search scope and improve work efficiency.

Optimization of Chromatographic Conditions for Detecting Ellagic Acid in Pomegranate Peels Using HPLC Method

[Objective] This study aimed to optimize the chromatographic conditions for detecting ellagic acid in pomegranate peels using HPLC method. [Method] By using 0.2 mg/ml ellagic acid standard solution, on the basis of single-factor experiment and orthogonal experiment, chromatographic conditions （mobile phase ratio, flow rate, col- umn temperature） for detecting ellagic acid using HPLC were optimized. Based on the optimal chromatographic conditions, the ellagic acid content in experimental pomegranate peels was determined. [Resull] The optimal chromatographic conditions for detecting ellagic acid in pomegranate peels using HPLC method are： 1.2% phos- phoric acid：acetonitrile=85：15, column temperature of 35 ℃, and flow rate of 1.0 ml/min. The linear regression equation of ellagic acid is： y=2.9e＋0.6x＋4.4e＋5 （FF=9 999）. Ac- cording to the standard addition recovery test, the average recovery rate of ellagic acid is 98.20%, and RSD is 0.60%. Under above optimized chromatographic condi- tions, ellagic acid can be well separated from other interfering components in pomegranate peels, with shorter peak time and ideal effect, which is convenient for the detection in production practices. [Conclusion] This study laid the foundation for detecting ellagic acid in pomegranate peels using HPLC method.

Joint DOA Estimation and Phase Calibration for Synchronous CDMA with Decorrelator

A joint direction of arrival (DOA) estimation and phase calibration for synchronous CDMA system with decorrelator are presented. Through decorrelating processing DOAs of the desired users can be estimated independently and all other resolved signal interferences are eliminated. Emphasis is directed to applications in which sensor phases may be in error. It is shown that accurate phase calibration in conjunction with their use in high resolution DOA estimation can be achieved for the decoupled signals.

Study on the scales of heterogeneous geologic bodies in random media

In order to study the scale characteristics of heterogeneities in complex media, a random medium is constructed using a statistical method and by changing model parameters （autocorrelation lengths a and b）, the scales of heterogeneous geologic bodies in the horizontal and the vertical Cartesian directions may be varied in the medium. The autocorrelation lengths a and b represent the mean scale of heterogeneous geologic bodies in the horizontal and vertical Cartesian directions in the randQm medium, respectively. Based on this model, the relationship between model autocorrelation lengths and heterogeneous geologic body scales is studied by horizontal velocity variation and standard deviation. The horizontal velocity variation research shows that velocities are in random perturbation. The heterogeneous geologic body scale increases with increasing autocorrelation length. The recursion equation for the relationship between autocorrelation lengths and heterogeneous geologic body scales is determined from the velocity standard deviation research and the actual heterogeneous geologic body scale magnitude can be estimated by the equation.

Development of Bio-fertilizer Standard in China and Establishment of Standard System

Over the past decade,national standard and industry standard of bio-fertilizer have been gradually established in China. Moreover,the microbial fertilizer standard system with Chinese characteristics has been firstly established by China in the world. Development of bio-fertilizer standard,especially the amplification and modification of bio-fertilizer product standard and the construction of bio-fertilizer standard system,etc. were summarized in this paper. Meanwhile,relative suggestions of existing problems were put forward.

Screening and Application of Molecular Markers for Starch Synthesis-related Genes in Rice

In this study, the genotypes of starch synthesis-related genes were systematically screened from different rice varieties using molecular markers. The results showed that starch synthesis-related genes were highly polymorphic between indica and japonica varieties, as they greatly variated among indica varieties, but were conserved among japonica varieties. The genotypes of two indica varieties9311 and Minghui 63 were more similar to that of japonica varieties. Two or three alleles of six starch synthesis-related genes were found in 28 japonica parental varieties. Four genotypes of two soluble starch synthase genes, SSIIa and SSIIIa,were detected in 88 stable lines derived from the cross of Kanto 194/ Wujing 13 using molecular markers.

Clustering Analysis on Large Grained Brassica napus Materials Based on the Optimized ACGM Markers

[Objective] This study aimed to develop ACGM markers for the clustering analysis of large grained Brassica napus materials. [Method] A total of 44 pairs of ACGM primers were designed according to 18 genes related to Arabidopsis grain development and their homologous rape EST sequences. After electrophoresis, 18 pairs of ACGM primers were selected for the clustering analysis of 16 larger grained samples and four fine grained samples of rapeseed. [Result] PCR result showed that 2-6 specific bands were respectively amplified by each pair of primes, and all the bands were polymorphic and repeatable, suggesting that the optimized ACGM markers were useful for clustering analysis of B. napus species. Clustering analysis revealed that the 20 rapeseed samples were divided into three clusters A, B, and C at similarity coefficient 0.6. Then, the clusters A and B were further divided into five sub clusters A1, A2, A3, B1 and B2 at similarity coefficient 0.67. [Conclusion] This study will provide theoretical and practical values for rape breeding.

Enabling weakened hedges in linguistic multi-criteria decision making

A semantics-based model is proposed to enable weakened hedges, such as ＂more or less＂ and ＂roughly＂ in the context of linguistic multi-criteria decision making. First, the resemblance relations are defined based on the semantics of terms on the domain. Then, the hedges can be represented after the upper and loose upper approximations of a linguistic term are derived. Accordingly, some compact formulae can be derived for the semantics of linguistic expressions with hedges. Parameters in these formulae are objectively determined according to the semantics of original terms. The proposed model presents a more natural way to express the decision information under uncertainties and its semantics is clear. The proposed model is clarified by solving the problem of evaluation and selection of sustainable innovative energy technologies. Computational results demonstrate that the model can deal with various uncertainties of the problem. Finally, the model is compared with existing techniques and extended to the case when the semantics of terms are represented by trapezoidal fuzzy numbers.

NOVEL GENERALIZED GREY INCIDENCE MODEL BASED ON INTERVAL GREY NUMBERS

To extend the traditional generalized grey incidence model, a novel grey incidence model based on inter- val grey numbers is constructed. Considering the numerical information of indexes cannot be accurately obtained and can be defined as interval grey numbers, the interval grey numbers are defined as standard interval grey num- bers which are split in white part and grey part. The absolute degree of incidence and relative degree of incidence based on the interval grey numbers are constructed and their arithmetic are given. Finally, an example about commercial aircraft index selection illuminates the effectiveness of the model. The results show that the model can sort indexes better and can extend the grey incidence models significantly.