目的探讨帕金森病(PD)患者皮肤小神经纤维的病理特点。方法收集16例临床确诊并符合纳入和排除标准的PD患者和性别年龄匹配的16例健康对照者的临床资料和神经电生理结果,对PD症状及周围神经病变进行评估。所有受试者行小腿皮肤活组织检查...目的探讨帕金森病(PD)患者皮肤小神经纤维的病理特点。方法收集16例临床确诊并符合纳入和排除标准的PD患者和性别年龄匹配的16例健康对照者的临床资料和神经电生理结果,对PD症状及周围神经病变进行评估。所有受试者行小腿皮肤活组织检查,通过标准化泛轴突标记蛋白基因产物9.5(PGP9.5)和生长相关蛋白43(GAP-43)两种免疫组织化学染色定量表皮内神经纤维密度(Intraepidermal nerve fiber density,IENFD),并进行统计学分析。结果16例PD患者,男7例,女9例,年龄(66.000±9.675)岁,Hoehn and Yahr(H-Y)分期(1.906±0.612)。PD组尺神经、正中神经、腓肠神经远端感觉潜伏期较对照组延长(P<0.05),余2组间神经电生理结果未见统计学差异(P>0.05)。PD组PGP9.5和GAP-43染色下IENFD值均低于健康对照组(8.618±3.984根/mm vs18.198±5.387根/mm,4.543±3.363根/mm vs 14.174±5.485根/mm;P<0.05)。两种染色下IENFD值与PD的临床症状有相关性。GAP-43/PGP9.5染色下IENFD比值在2组之间无统计学差异(0.575±0.380 vs 0.787±0.218;P>0.05)。结论PD患者存在周围神经损害,以小神经纤维受累为主,大直径神经纤维受累不明显,皮肤活检是诊断PD小纤维神经病较为可靠的方法。展开更多
Sea cucumber Apostichopus japonicus samples were collected in Changdao, Penglai (PL), 27 individuals, and Lingshandao, Qingdao (QD), 30 individuals, in the Shandong Peninsula, China. Ten SSR primers were used to asses...Sea cucumber Apostichopus japonicus samples were collected in Changdao, Penglai (PL), 27 individuals, and Lingshandao, Qingdao (QD), 30 individuals, in the Shandong Peninsula, China. Ten SSR primers were used to assess the genetic variation and relationship between and within the two stocks. Respectively, for each stock, the percentage of polymorphic bands was 85.2% and 86.9%; the gene diver- sity was 0.360 5 and 0.342 8; and the Shannon’s information index was 0.515 0 and 0.499 0. At species level, the percentage of polymorphic bands was 92.2%, the total gene diversity was 0.378 9 and the Shannon’s information index was 0.550 4. The coefficient of overall genetic differentiation and the ge- netic distances between the stocks were also calculated to be 0.073 0 and 0.079 6 using the POPGENE program. Results show that the genetic diversity of the two stocks is still large but the genetic distance between the two stocks is close. A dendrogram was constructed for the 57 individuals from the two stocks, showing that the genetic structure was unitary for PL stock but complex for QD stock.展开更多
This work is devoted to obtaining radionuclide-labeled microspheres for radionuclide therapy as a therapeutic drug with a selective method of delivery. The nature of magnetic microspheres and quantitative elemental co...This work is devoted to obtaining radionuclide-labeled microspheres for radionuclide therapy as a therapeutic drug with a selective method of delivery. The nature of magnetic microspheres and quantitative elemental composition radiopreparats was considered. We have developed a new method of producing radionuclide-labeled microspheres （radiopharmaceutical ^140Nd-microspheres） for radionuclide therapy.展开更多
Statistical classification methods are frequently applied to analyze metabolomics data, especially from medicinal plants. Combined with variable selection techniques, we are able to identify marker candidates, which c...Statistical classification methods are frequently applied to analyze metabolomics data, especially from medicinal plants. Combined with variable selection techniques, we are able to identify marker candidates, which can be used to discriminate the group to which unknown subjects belong. After preprocessing, such as outlier checking, normalization, missing value imputation and transformation, we then mainly utilized four novel classification methods： RF （random forest）, NSC （nearest shrunken centroid）, PLS-DA （partial least square discriminant analysis） and SAM （significant analysis ofmicroarrays）. Each method has its own device to measure the importance of single metabolite, so that, it is probable to choose highly ranked metabolites, which show the best prediction accuracy. Adapting above strategy, we have successfully analyzed several kinds of metabolomics data including Panax ginseng, Lespedeza species, Anemarrhean asphodeloides and Gastrodia elata.展开更多
p-Quinols are ubiquitous structural motifs of various natural products and pharmaceutical compounds,and versatile building blocks in synthetic chemistry.The reported methods for the synthesis of p-quinol require stoic...p-Quinols are ubiquitous structural motifs of various natural products and pharmaceutical compounds,and versatile building blocks in synthetic chemistry.The reported methods for the synthesis of p-quinol require stoichiometric amounts of oxidants.Molecular oxygen is considered as an ideal oxidant due to its natural,inexpensive,and environmentally friendly characteristics.During the ongoing research of C–H bond hydroxylation,we found that multi-alkyl phenols could react with molecular oxygen under mild conditions.Herein,we describe an efficient oxidative de-aromatization of multi-alkyl phenols to p-quinols.1 atm of molecular oxygen was used as the oxidant.Many multi-alkyl phenols could react smoothly at room temperature.Isotopic labeling experiment was also performed,and the result proved that the oxygen atom in the produced hydroxyl group is from molecular oxygen.展开更多
文摘目的探讨帕金森病(PD)患者皮肤小神经纤维的病理特点。方法收集16例临床确诊并符合纳入和排除标准的PD患者和性别年龄匹配的16例健康对照者的临床资料和神经电生理结果,对PD症状及周围神经病变进行评估。所有受试者行小腿皮肤活组织检查,通过标准化泛轴突标记蛋白基因产物9.5(PGP9.5)和生长相关蛋白43(GAP-43)两种免疫组织化学染色定量表皮内神经纤维密度(Intraepidermal nerve fiber density,IENFD),并进行统计学分析。结果16例PD患者,男7例,女9例,年龄(66.000±9.675)岁,Hoehn and Yahr(H-Y)分期(1.906±0.612)。PD组尺神经、正中神经、腓肠神经远端感觉潜伏期较对照组延长(P<0.05),余2组间神经电生理结果未见统计学差异(P>0.05)。PD组PGP9.5和GAP-43染色下IENFD值均低于健康对照组(8.618±3.984根/mm vs18.198±5.387根/mm,4.543±3.363根/mm vs 14.174±5.485根/mm;P<0.05)。两种染色下IENFD值与PD的临床症状有相关性。GAP-43/PGP9.5染色下IENFD比值在2组之间无统计学差异(0.575±0.380 vs 0.787±0.218;P>0.05)。结论PD患者存在周围神经损害,以小神经纤维受累为主,大直径神经纤维受累不明显,皮肤活检是诊断PD小纤维神经病较为可靠的方法。
基金Supported by the NSFC (No. 30571417)The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China (No. 200426)
文摘Sea cucumber Apostichopus japonicus samples were collected in Changdao, Penglai (PL), 27 individuals, and Lingshandao, Qingdao (QD), 30 individuals, in the Shandong Peninsula, China. Ten SSR primers were used to assess the genetic variation and relationship between and within the two stocks. Respectively, for each stock, the percentage of polymorphic bands was 85.2% and 86.9%; the gene diver- sity was 0.360 5 and 0.342 8; and the Shannon’s information index was 0.515 0 and 0.499 0. At species level, the percentage of polymorphic bands was 92.2%, the total gene diversity was 0.378 9 and the Shannon’s information index was 0.550 4. The coefficient of overall genetic differentiation and the ge- netic distances between the stocks were also calculated to be 0.073 0 and 0.079 6 using the POPGENE program. Results show that the genetic diversity of the two stocks is still large but the genetic distance between the two stocks is close. A dendrogram was constructed for the 57 individuals from the two stocks, showing that the genetic structure was unitary for PL stock but complex for QD stock.
文摘This work is devoted to obtaining radionuclide-labeled microspheres for radionuclide therapy as a therapeutic drug with a selective method of delivery. The nature of magnetic microspheres and quantitative elemental composition radiopreparats was considered. We have developed a new method of producing radionuclide-labeled microspheres （radiopharmaceutical ^140Nd-microspheres） for radionuclide therapy.
文摘Statistical classification methods are frequently applied to analyze metabolomics data, especially from medicinal plants. Combined with variable selection techniques, we are able to identify marker candidates, which can be used to discriminate the group to which unknown subjects belong. After preprocessing, such as outlier checking, normalization, missing value imputation and transformation, we then mainly utilized four novel classification methods： RF （random forest）, NSC （nearest shrunken centroid）, PLS-DA （partial least square discriminant analysis） and SAM （significant analysis ofmicroarrays）. Each method has its own device to measure the importance of single metabolite, so that, it is probable to choose highly ranked metabolites, which show the best prediction accuracy. Adapting above strategy, we have successfully analyzed several kinds of metabolomics data including Panax ginseng, Lespedeza species, Anemarrhean asphodeloides and Gastrodia elata.
基金supported by the National Natural Science Foundation of China(21325206,21172006)the Key Laboratory of Oil and Gas Fine Chemicals(XJDX0908-2013-2)
文摘p-Quinols are ubiquitous structural motifs of various natural products and pharmaceutical compounds,and versatile building blocks in synthetic chemistry.The reported methods for the synthesis of p-quinol require stoichiometric amounts of oxidants.Molecular oxygen is considered as an ideal oxidant due to its natural,inexpensive,and environmentally friendly characteristics.During the ongoing research of C–H bond hydroxylation,we found that multi-alkyl phenols could react with molecular oxygen under mild conditions.Herein,we describe an efficient oxidative de-aromatization of multi-alkyl phenols to p-quinols.1 atm of molecular oxygen was used as the oxidant.Many multi-alkyl phenols could react smoothly at room temperature.Isotopic labeling experiment was also performed,and the result proved that the oxygen atom in the produced hydroxyl group is from molecular oxygen.
