[Objective] SSR molecular marker technique was used to determine the purity of sunflower seed with the aim to provide accurate, convenient method for the identification of the purity of hybrid seeds in production and ...[Objective] SSR molecular marker technique was used to determine the purity of sunflower seed with the aim to provide accurate, convenient method for the identification of the purity of hybrid seeds in production and processing. [Method] With the DNA of Xinshikui 6 and its parents as template, about 100 pairs of SSR molecular markers were screened after DNA extraction, PCR amplification and electrophoresis production. [Results] SSR polymorphic primer marker 532 produced a specific band of 469 bp in the female parent, and a specific band of 451 bp in the male parent; primer marker 574 produced a specific band of 364 bp in the female parent, and a specific band of 384 bp in the male parent. The indoor molecular purity identification and field purity identification were consistent with each other. The primer marker 532 and 574 could be obtained from the SSR molecular marker method to distinguish the male parent, female parent and hybrid of Xinshikui 6, and both of the 2 primer markers can effectively identify the purities of the hybrid seeds of Xinshikui 6, as well as the authenticity of the seeds. [Conclusion] The proposed method was simple, fast, accurate to operate with the advantages of high reproducibility, and it had become the major method in the identification of sunflower varieties.展开更多
[Objective] This study aimed to establish a method for rapid identification of Ningza 11 seeds purity with SSR markers. [Method] Taking Ningza 11 hybrid seeds as experimental materials, a method for rapid identificati...[Objective] This study aimed to establish a method for rapid identification of Ningza 11 seeds purity with SSR markers. [Method] Taking Ningza 11 hybrid seeds as experimental materials, a method for rapid identification of hybrid rape-seeds was established with SSR molecular markers; meanwhile, the test seeds were planted in the field for comparison and verification. [Result] A method for rapid identification of Ningza 11 seeds purity with SSR molecular markers was estab-lished： DNA from seeds germinated in the night was extracted by alkaline lysis method; the PCR amplification was performed for 2 h, and electrophoresis for 1.5 h, and a silver staining for 10 minutes. It took less than one day to from obtaining sampling seeds to obtaining the purity identification result, so a skil ed professional can complete the detection of at least 6 ×96 = 576 seeds per weekday. By using this set of detection system, the measured purity of seeds from nine samples was extremely significantly positively correlated to the actual purity identified in the field test, with a correlation coefficient of up to 0.984 （P〈0.01）. [Conclusion] This SSR-PCR molecular identification system can be applied for rapid and accurate identifi-cation of Ningza 11 hybrid seeds.展开更多
Hybrid rice Xinhunyou No. 6 could be cultivated by mixing male and fe- male parents and performing seedling raising followed by transplanting, mechanical planting or mechanical direct seeding according to the producti...Hybrid rice Xinhunyou No. 6 could be cultivated by mixing male and fe- male parents and performing seedling raising followed by transplanting, mechanical planting or mechanical direct seeding according to the production methods of com- mercial rice. During flowering stage, leaf cutting, application of ＂920＂ and pollination were conducted; after pollination, bentazon with a certain concentration was sprayed to kill the male parent; and the hybrid rice was harvested mechanically. Before storage, color separation was performed to remove little remaining male parent, thereby achieving whole-process mechanization of hybrid rice seed production. This study introduced mechanized seed production of Xinhunyou No. 6 through mixed- seeding from the aspects including mechanical direct seeding, seeding raising fol- lowed by transplanting and mechanical planting.展开更多
Objective To investigate the role of sphingosine-l-phosphate （S1P） and its receptors in cardiomyocyte autophagy, cardiomyocyte hypertrophy and cardiac function. Methods Cardiomyocytes were isolated from neonatal Vis...Objective To investigate the role of sphingosine-l-phosphate （S1P） and its receptors in cardiomyocyte autophagy, cardiomyocyte hypertrophy and cardiac function. Methods Cardiomyocytes were isolated from neonatal Vista rats. Autophagy and hypertrophy of car- diomyocytes were induced via starvation culture and phenylephrine （PE）, respectively, and S 1 P was used to treat the cardiomyocytes. The effect of S1P on cardiomyocyte autophagy was evaluated by the number of autophagosomes, the expression of autophagy-related proteins and autophagic marker genes in cardiomyocytes. The effect of S1P on cardiomyocyte hypertrophy was evaluated by examining the surface area of cardiomyoeytes and the expression of hypertrophic genes. Subsequently, different small interfering RNAs （siRNAs） were used to knockdown the expression of the three types of S 1P receptors on cardiomyocytes and to analyze the type of receptor that mediates S 1P sig- naling in cardiomyocytes. Finally, sphingosine 1 phosphate receptor-1 （S1PR1） was knockout in the mouse cardiomyocytes using the Cas9 technique. The effect of S 1PR1 on cardiac autophagy and cardiac hypertrophy was examined by assessing cardiomyocyte autophagy, car- diomyocyte hypertrophy and cardiac function. Results Starvation-induced cardiomyocyte autophagy and PE -induced cardiomyocyte hy- pertrophy were significantly attenuated by SIP. The results showed that the formation of autophagosomes was decreased, the auto- phagy-associated protein LC3 II/I and the expression of autophagic marker genes Atg5, Atgl2, Beclinl and LC3B decreased after SIP treatment. The surface area of the cardiomyocytes was decreased, and the expression of hypertrophic genes, including atrial natriuretic factor （ANF）, skeletal muscle and cardiac actin （SKA）, myosin heavy chain （β-MHC） and brain natriuretic peptide （BNP） were all decreased after S 1 P treatment. The autophagy and hypertrophy of cardiomyocytes in the S 1PR 1 knocked-down group were significantly increased compared to those in the control group, the SIPR2 and the S1PR3 knocked-down groups. In vivo, the knockout of S1PR1 in cardiomyocytes exacer- bated stress-induced cardiac autophagy, cardiac hypertrophy and the impairment of cardiac function. Conclusion SIP could inhibit car- diomyocyte autophagy, thereby inhibiting cardiomyocyte hypertrophy and protecting cardiac function by activating S1PR1 in pres- sure-overloaded cardiomyocytes in mice.展开更多
In vertebrates, the patterning of anterior-posterior （AP） axis is a fundamental process during embryogenesis. Wnt and FGF signalling pathways play important roles in regulating the patterning of embryo AP axis. Mous...In vertebrates, the patterning of anterior-posterior （AP） axis is a fundamental process during embryogenesis. Wnt and FGF signalling pathways play important roles in regulating the patterning of embryo AP axis. Mouse Tbx6 encodes a transcription factor that has been demonstrated to be involved in the specification of the posterior tissue in mouse embryonic body. Here, we prove that morpholino-induced knockdown of XTbx6 impairs posterior development, indicating the requirement of XTbx6 in this process. Meanwhile, gain of XTbx6 function is sufficient to induce ectopic posterior structures in Xenopus embryos. Furthermore, XTbx6 activates the expression of Xwnt8 and FGF8, which are two mediators of posterior development, suggesting a mechanism by which XTbx6 modulates posterior patterning via Wnt and FGF signalling pathway activation.展开更多
The sign algorithm has been extensively investigated for digital echo cancellation application and other adaptive filtering applications. In this paper, we use the blind averaging Sign-regressor (SR) algorithm for ada...The sign algorithm has been extensively investigated for digital echo cancellation application and other adaptive filtering applications. In this paper, we use the blind averaging Sign-regressor (SR) algorithm for adaptive multiuser detection. It is another least mean square (LMS) algorithm and eliminates the need for multiplication in the adaptive algorithm. The new algorithm not only reduces the calculation complexity but also has good convergence character. Simulations indicate that this algorithm can adapt to the changes of the environment quickly and improve the stability of the SIR.展开更多
Sixteen polymorphic microsatellite markers suitable for population genetic structure analysis and signal transduction coding genes variation measurement were developed for rice blast fungus, Magnaporthe grisea. Polymo...Sixteen polymorphic microsatellite markers suitable for population genetic structure analysis and signal transduction coding genes variation measurement were developed for rice blast fungus, Magnaporthe grisea. Polymorphism was evaluated by using forty-six isolates collected from diverse geographical locations （including japonica grown zone, indica grown zone） and rice varieties. Preliminary results indicated that each locus resolved multiple alleles ranging from three to fourteen. The results showed that these SSR-containing genes are also polymorphic in the nature population.展开更多
基金Supported by the Key Science and Technology Project of Xinjiang Production and Construction Corps(2016AC024)the Key Science and Technology Project for Seed Breeding during the Thirteenth Five Year Plan of Xinjiang Production and Construction Corps(2014BA005)+1 种基金the China Agriculture Research System for Sunflower of China(CARS-16)the Science and Technology Project for Supporting Xinjiang of China(2014AB007)~~
文摘[Objective] SSR molecular marker technique was used to determine the purity of sunflower seed with the aim to provide accurate, convenient method for the identification of the purity of hybrid seeds in production and processing. [Method] With the DNA of Xinshikui 6 and its parents as template, about 100 pairs of SSR molecular markers were screened after DNA extraction, PCR amplification and electrophoresis production. [Results] SSR polymorphic primer marker 532 produced a specific band of 469 bp in the female parent, and a specific band of 451 bp in the male parent; primer marker 574 produced a specific band of 364 bp in the female parent, and a specific band of 384 bp in the male parent. The indoor molecular purity identification and field purity identification were consistent with each other. The primer marker 532 and 574 could be obtained from the SSR molecular marker method to distinguish the male parent, female parent and hybrid of Xinshikui 6, and both of the 2 primer markers can effectively identify the purities of the hybrid seeds of Xinshikui 6, as well as the authenticity of the seeds. [Conclusion] The proposed method was simple, fast, accurate to operate with the advantages of high reproducibility, and it had become the major method in the identification of sunflower varieties.
基金Supported by the Jiangsu Provincial Agricultural Science and Technology Innovation Fund[CX(11)1026]National Science and Technology Support Program(2010BAD01B-10)863 Major Project(2011AA10A10403)~~
文摘[Objective] This study aimed to establish a method for rapid identification of Ningza 11 seeds purity with SSR markers. [Method] Taking Ningza 11 hybrid seeds as experimental materials, a method for rapid identification of hybrid rape-seeds was established with SSR molecular markers; meanwhile, the test seeds were planted in the field for comparison and verification. [Result] A method for rapid identification of Ningza 11 seeds purity with SSR molecular markers was estab-lished： DNA from seeds germinated in the night was extracted by alkaline lysis method; the PCR amplification was performed for 2 h, and electrophoresis for 1.5 h, and a silver staining for 10 minutes. It took less than one day to from obtaining sampling seeds to obtaining the purity identification result, so a skil ed professional can complete the detection of at least 6 ×96 = 576 seeds per weekday. By using this set of detection system, the measured purity of seeds from nine samples was extremely significantly positively correlated to the actual purity identified in the field test, with a correlation coefficient of up to 0.984 （P〈0.01）. [Conclusion] This SSR-PCR molecular identification system can be applied for rapid and accurate identifi-cation of Ningza 11 hybrid seeds.
基金Supported by Science and Technology Innovation Team of Anhui Academy of Agricultural Sciences(15C0108)Subject Construction Project of Anhui Academy of Agricultural Sciences(14A0102)Science and Technology Support Program of Ministry of Science and Technology(2012BAD07B01-3)~~
文摘Hybrid rice Xinhunyou No. 6 could be cultivated by mixing male and fe- male parents and performing seedling raising followed by transplanting, mechanical planting or mechanical direct seeding according to the production methods of com- mercial rice. During flowering stage, leaf cutting, application of ＂920＂ and pollination were conducted; after pollination, bentazon with a certain concentration was sprayed to kill the male parent; and the hybrid rice was harvested mechanically. Before storage, color separation was performed to remove little remaining male parent, thereby achieving whole-process mechanization of hybrid rice seed production. This study introduced mechanized seed production of Xinhunyou No. 6 through mixed- seeding from the aspects including mechanical direct seeding, seeding raising fol- lowed by transplanting and mechanical planting.
文摘Objective To investigate the role of sphingosine-l-phosphate （S1P） and its receptors in cardiomyocyte autophagy, cardiomyocyte hypertrophy and cardiac function. Methods Cardiomyocytes were isolated from neonatal Vista rats. Autophagy and hypertrophy of car- diomyocytes were induced via starvation culture and phenylephrine （PE）, respectively, and S 1 P was used to treat the cardiomyocytes. The effect of S1P on cardiomyocyte autophagy was evaluated by the number of autophagosomes, the expression of autophagy-related proteins and autophagic marker genes in cardiomyocytes. The effect of S1P on cardiomyocyte hypertrophy was evaluated by examining the surface area of cardiomyoeytes and the expression of hypertrophic genes. Subsequently, different small interfering RNAs （siRNAs） were used to knockdown the expression of the three types of S 1P receptors on cardiomyocytes and to analyze the type of receptor that mediates S 1P sig- naling in cardiomyocytes. Finally, sphingosine 1 phosphate receptor-1 （S1PR1） was knockout in the mouse cardiomyocytes using the Cas9 technique. The effect of S 1PR1 on cardiac autophagy and cardiac hypertrophy was examined by assessing cardiomyocyte autophagy, car- diomyocyte hypertrophy and cardiac function. Results Starvation-induced cardiomyocyte autophagy and PE -induced cardiomyocyte hy- pertrophy were significantly attenuated by SIP. The results showed that the formation of autophagosomes was decreased, the auto- phagy-associated protein LC3 II/I and the expression of autophagic marker genes Atg5, Atgl2, Beclinl and LC3B decreased after SIP treatment. The surface area of the cardiomyocytes was decreased, and the expression of hypertrophic genes, including atrial natriuretic factor （ANF）, skeletal muscle and cardiac actin （SKA）, myosin heavy chain （β-MHC） and brain natriuretic peptide （BNP） were all decreased after S 1 P treatment. The autophagy and hypertrophy of cardiomyocytes in the S 1PR 1 knocked-down group were significantly increased compared to those in the control group, the SIPR2 and the S1PR3 knocked-down groups. In vivo, the knockout of S1PR1 in cardiomyocytes exacer- bated stress-induced cardiac autophagy, cardiac hypertrophy and the impairment of cardiac function. Conclusion SIP could inhibit car- diomyocyte autophagy, thereby inhibiting cardiomyocyte hypertrophy and protecting cardiac function by activating S1PR1 in pres- sure-overloaded cardiomyocytes in mice.
基金the National Natural Science Foundation of China （90408005, 30270650） the National Key Project for Basic Science Research of China （2001CB509901）.
文摘In vertebrates, the patterning of anterior-posterior （AP） axis is a fundamental process during embryogenesis. Wnt and FGF signalling pathways play important roles in regulating the patterning of embryo AP axis. Mouse Tbx6 encodes a transcription factor that has been demonstrated to be involved in the specification of the posterior tissue in mouse embryonic body. Here, we prove that morpholino-induced knockdown of XTbx6 impairs posterior development, indicating the requirement of XTbx6 in this process. Meanwhile, gain of XTbx6 function is sufficient to induce ectopic posterior structures in Xenopus embryos. Furthermore, XTbx6 activates the expression of Xwnt8 and FGF8, which are two mediators of posterior development, suggesting a mechanism by which XTbx6 modulates posterior patterning via Wnt and FGF signalling pathway activation.
文摘The sign algorithm has been extensively investigated for digital echo cancellation application and other adaptive filtering applications. In this paper, we use the blind averaging Sign-regressor (SR) algorithm for adaptive multiuser detection. It is another least mean square (LMS) algorithm and eliminates the need for multiplication in the adaptive algorithm. The new algorithm not only reduces the calculation complexity but also has good convergence character. Simulations indicate that this algorithm can adapt to the changes of the environment quickly and improve the stability of the SIR.
文摘Sixteen polymorphic microsatellite markers suitable for population genetic structure analysis and signal transduction coding genes variation measurement were developed for rice blast fungus, Magnaporthe grisea. Polymorphism was evaluated by using forty-six isolates collected from diverse geographical locations （including japonica grown zone, indica grown zone） and rice varieties. Preliminary results indicated that each locus resolved multiple alleles ranging from three to fourteen. The results showed that these SSR-containing genes are also polymorphic in the nature population.
