Objective: To investigate the predictability of MRI and the possiblebiological markers of cavernous sinus invasion of pituitary adenomas associated with fourphenomenas: angiogenesis, cell proliferation, apoptosis and ...Objective: To investigate the predictability of MRI and the possiblebiological markers of cavernous sinus invasion of pituitary adenomas associated with fourphenomenas: angiogenesis, cell proliferation, apoptosis and matrix metalloproteinase. Methods: Weevaluated 45 patients with pituitary adenoma according to the MRI, surgical findings and theimmunohistochemistry staining of tumor tissues. Results: The results have shown that the sensitivityof MRI for predicting cavernous sinus invasion in this prospective study was 60%, its specificity85%, its positive predictive value 83.33%, negative predictive value 62.96%. 45 specimens ofpituitary adenomas were analyzed for expression of F8, VEGF, Ki-67, c-myc, Bcl-2, nm23 and MMP-9immunoreactivity using immunoperoxidase staining. MVD was assessed using F8-related antigen. Theresults have shown that MVD of invasive pituitary adenomas was significantly higher than that ofnoninvasive (P 【 0.001). There was an association between the invasion of pituitary adenomas andKi-67 LI (P = 0.039) or the expression of VEGF (P 【 0.001) and MMP-9 (P 【 0.001). But c-myc LI andBcl-2 expression have no association with invasiveness of pituitary adenomas (P = 0.061 versus P =0.201). On the other hand, there is an inverse relationship between nm23 expression and tumorinvasion (P 【 0.001). Conclusion: Parasellar extension of pituitary adenomas through the medial wallof the cavernous sinus is diagnosed at surgery, and with sensitive gadolinium-enhanced MRI, itsextent can be partly determined by radiology. Although our study has shown that MVD and theexpression of VEGF, Ki-67, nm23 and MMP-9 have associations with invasiveness of pituitary adenomas,they are lack of specificity. These markers can only provide some useful information.展开更多
DSA images show vessels with clarity and CT images show bones distinctly. In this paper, we present an experimental research on the frameless registration of DSA/CT images based on localization algorithm. With four ex...DSA images show vessels with clarity and CT images show bones distinctly. In this paper, we present an experimental research on the frameless registration of DSA/CT images based on localization algorithm. With four external markers, the vessels and bones in human brain can be integrated. The mean accuracy of simulated experiment is about 2.0 mm. The experiment proved that the 3D images composed cerebral anatomy and vasculature could help neurosurgeons perform accurate diagnosis and make right operation planning.展开更多
AIM:To identify and assess the novel makers for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 with an integrated computational and experimental approach. METHODS:High-throughput NCBI blast (E-valu...AIM:To identify and assess the novel makers for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 with an integrated computational and experimental approach. METHODS:High-throughput NCBI blast (E-value cutoff e-5) was used to search homologous genes among all sequenced prokaryotic genomes of each gene encoded in each of the three strains of STEC O157:H7 with complete genomes,aiming to find unique genes in O157:H7 as its potential markers. To ensure that the identified markers from the three strains of STEC O157:H7 can serve as general markers for all the STEC O157:H7 strains,a genomic barcode approach was used to select the markers to minimize the possibility of choosing a marker gene as part of a transposable element. Effectiveness of the markers predicted was then validated by running polymerase chain reaction (PCR) on 18 strains of O157:H7 with 5 additional genomes used as negative controls. RESULTS:The blast search identified 20,16 and 20 genes,respectively,in the three sequenced strains of STEC O157:H7,which had no homologs in any of the other prokaryotic genomes. Three genes,wzy,Z0372 and Z0344,common to the three gene lists,were selected based on the genomic barcode approach. PCR showed an identification accuracy of 100% on the 18 tested strains and the 5 controls. CONCLUSION:The three identified novel markers,wzy,Z0372 and Z0344,are highly promising for the detection of STEC O157:H7,in complementary to the known markers.展开更多
In order to monitor the deformations of modern constructions a local 3D network was usually being established at the surrounding area. The centering error, of both the instrument and targets, causes significant uncert...In order to monitor the deformations of modern constructions a local 3D network was usually being established at the surrounding area. The centering error, of both the instrument and targets, causes significant uncertainties in the determination of the x, y, z coordinates of the network＇s points. In order to assure precise centering for both the instrument and targets, not only for the accessible but also for the inaccessible network＇s points, a prototype way of marking is being implied. A special semi-permanent portable metallic stand （Photo 1） was manufactured for marking the accessible points. The stand provides forced instrument centering of the order of_＋0, lmm. It is light enough to carry, it accelerates and facilitates the centering and leveling of the instrument as well as it eliminates the time needed for the measurements. The applied laboratory checks in order to certify the suitability of its use and the provided accuracy are being described. For the inaccessible points special targets were used. The targets were put in permanent attachments （Photos 7-8）, which were also manufactured. Useful conclusions were drawn when these special accessories were used in to a 3D network, which was established for the monitoring of a new football stadium. Two measurement phases were carried out. The first one is with the stadium empty and the second one is when it was crowded （about 32000 people） during a significant football match.展开更多
文摘Objective: To investigate the predictability of MRI and the possiblebiological markers of cavernous sinus invasion of pituitary adenomas associated with fourphenomenas: angiogenesis, cell proliferation, apoptosis and matrix metalloproteinase. Methods: Weevaluated 45 patients with pituitary adenoma according to the MRI, surgical findings and theimmunohistochemistry staining of tumor tissues. Results: The results have shown that the sensitivityof MRI for predicting cavernous sinus invasion in this prospective study was 60%, its specificity85%, its positive predictive value 83.33%, negative predictive value 62.96%. 45 specimens ofpituitary adenomas were analyzed for expression of F8, VEGF, Ki-67, c-myc, Bcl-2, nm23 and MMP-9immunoreactivity using immunoperoxidase staining. MVD was assessed using F8-related antigen. Theresults have shown that MVD of invasive pituitary adenomas was significantly higher than that ofnoninvasive (P 【 0.001). There was an association between the invasion of pituitary adenomas andKi-67 LI (P = 0.039) or the expression of VEGF (P 【 0.001) and MMP-9 (P 【 0.001). But c-myc LI andBcl-2 expression have no association with invasiveness of pituitary adenomas (P = 0.061 versus P =0.201). On the other hand, there is an inverse relationship between nm23 expression and tumorinvasion (P 【 0.001). Conclusion: Parasellar extension of pituitary adenomas through the medial wallof the cavernous sinus is diagnosed at surgery, and with sensitive gadolinium-enhanced MRI, itsextent can be partly determined by radiology. Although our study has shown that MVD and theexpression of VEGF, Ki-67, nm23 and MMP-9 have associations with invasiveness of pituitary adenomas,they are lack of specificity. These markers can only provide some useful information.
基金Supported by NSF(No.30570511) and National Fund for the Doctoral Program(No.200350)
文摘DSA images show vessels with clarity and CT images show bones distinctly. In this paper, we present an experimental research on the frameless registration of DSA/CT images based on localization algorithm. With four external markers, the vessels and bones in human brain can be integrated. The mean accuracy of simulated experiment is about 2.0 mm. The experiment proved that the 3D images composed cerebral anatomy and vasculature could help neurosurgeons perform accurate diagnosis and make right operation planning.
基金Supported by National Natural Science Foundation of China, No. 30872415National Science Foundation, No. DBI-0354771, ITR-IIS-0407204, DBI-0542119, CCF0621700+1 种基金National Institutes of Health, No. 1R01GM075331 and 1R01GM081682BioEnergy Science Center, US Department of Energy BioEnergy Research Center supported by the Office of Biological and Environmental Research in DOE Office of Science
文摘AIM:To identify and assess the novel makers for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 with an integrated computational and experimental approach. METHODS:High-throughput NCBI blast (E-value cutoff e-5) was used to search homologous genes among all sequenced prokaryotic genomes of each gene encoded in each of the three strains of STEC O157:H7 with complete genomes,aiming to find unique genes in O157:H7 as its potential markers. To ensure that the identified markers from the three strains of STEC O157:H7 can serve as general markers for all the STEC O157:H7 strains,a genomic barcode approach was used to select the markers to minimize the possibility of choosing a marker gene as part of a transposable element. Effectiveness of the markers predicted was then validated by running polymerase chain reaction (PCR) on 18 strains of O157:H7 with 5 additional genomes used as negative controls. RESULTS:The blast search identified 20,16 and 20 genes,respectively,in the three sequenced strains of STEC O157:H7,which had no homologs in any of the other prokaryotic genomes. Three genes,wzy,Z0372 and Z0344,common to the three gene lists,were selected based on the genomic barcode approach. PCR showed an identification accuracy of 100% on the 18 tested strains and the 5 controls. CONCLUSION:The three identified novel markers,wzy,Z0372 and Z0344,are highly promising for the detection of STEC O157:H7,in complementary to the known markers.
文摘In order to monitor the deformations of modern constructions a local 3D network was usually being established at the surrounding area. The centering error, of both the instrument and targets, causes significant uncertainties in the determination of the x, y, z coordinates of the network＇s points. In order to assure precise centering for both the instrument and targets, not only for the accessible but also for the inaccessible network＇s points, a prototype way of marking is being implied. A special semi-permanent portable metallic stand （Photo 1） was manufactured for marking the accessible points. The stand provides forced instrument centering of the order of_＋0, lmm. It is light enough to carry, it accelerates and facilitates the centering and leveling of the instrument as well as it eliminates the time needed for the measurements. The applied laboratory checks in order to certify the suitability of its use and the provided accuracy are being described. For the inaccessible points special targets were used. The targets were put in permanent attachments （Photos 7-8）, which were also manufactured. Useful conclusions were drawn when these special accessories were used in to a 3D network, which was established for the monitoring of a new football stadium. Two measurement phases were carried out. The first one is with the stadium empty and the second one is when it was crowded （about 32000 people） during a significant football match.
