[Objective] The aim was to study the supporting raising technology of Castanea mollissima Blume molecular marker-assisted selection to reduce the cost and time for chestnut breeding.[Method] To find the best cutting m...[Objective] The aim was to study the supporting raising technology of Castanea mollissima Blume molecular marker-assisted selection to reduce the cost and time for chestnut breeding.[Method] To find the best cutting method,10 treatments were designed according to transverse diameter and vertical diameter of the chestnut including different cutting ratios in 1/2,1/3,1/4 and different directions about cross cutting,vertical cutting,bevel cutting and two side-cutting.The germination rate,seedling survival rate and morphological indicators of the treatments were measured;significant difference among them was also analyzed.[Result] 1/3 bevel cutting was the best,which not only could give high germination rate and survival rate,but also could guarantee high quality of nursery stock,which did not grow so differently with the untreated seedlings.[Conclusion] This way can not only reduce too much cost and time for seedling breeding,but also promote the germination of chestnut in advance,which could do much good to production.展开更多
Although stone palettes from the Naqada culture are well known, research has been focused mostly on those palettes which are richly decorated. However, streaks and fragments of individual signs are also often found on...Although stone palettes from the Naqada culture are well known, research has been focused mostly on those palettes which are richly decorated. However, streaks and fragments of individual signs are also often found on simple palettes of various shapes. This study shows that the majority of these palettes have quite a number of marks and sign fragments that have been made by different techniques and over a long period of time. This particularly applies to rhomboidal palettes, for most of which there are no ground to infer that they have been used for ritual purposes or for mixing beauty care compositions. It is more likely that they denote an early form of the visual symbol in later periods represented by the head of Ptah＇s staff.展开更多
A novel, cheap, disposable and single-use nanoparticles-based nanochannel platform assembled on a flexible substrate for label-free immunosensing is pre- sented. This sensing platform is formed by the dip-coating of a...A novel, cheap, disposable and single-use nanoparticles-based nanochannel platform assembled on a flexible substrate for label-free immunosensing is pre- sented. This sensing platform is formed by the dip-coating of a homogeneous and assembled monolayer of carboxylated polystyrene nanospheres （PS, 200 and 500 nm-sized） onto the working area of flexible screen-printed indium tin oxide/polyethylene terephthalate （ITO/PET） electrodes. The spaces between the self-assembled nanospheres generate well-ordered nanochannels, with inter-PS particles distances of around 65 and 24 nm respectively. The formed nanochannels are used for the effective immobilization of antibodies and subsequent protein detection based on the monitoring of [Fe（CN）6]^4- flow through diffusion and the decrease in the differential pulse voltammetric signal upon immunocomplex formation. The obtained sensing system is nanochannel-size dependent and allows human immunoglobulin G （IgG） （chosen as a model analyte） to be detected at levels of 580 ng/mL. The system also exhibits an excellent specificity against other proteins present in real samples and shows good performance with a human urine sample. The developed device represents an integrated and simple biodetection system which overcomes many of the limitations of previously reported nanochannels-based approaches and can be extended in the future to several other immuno and DNA detection systems.展开更多
文摘目的研究使用En Vision多标记读板仪检测细胞内钙信号的可行性。方法分别用卡巴胆碱和凝血酶刺激LN229、SKN-MC细胞或者磷脂酶C抑制剂U73122预处理的LN229细胞,使用En Vision多标记读板仪检测细胞内钙信号强度的变化。结果卡巴胆碱诱导的LN229细胞内钙信号变化呈现浓度依赖性;卡巴胆碱和凝血酶诱导的细胞内钙信号变化趋势不同;凝血酶诱导的钙信号变化具有细胞特异性;U73122对凝血酶诱导的钙信号的抑制作用呈现剂量依赖性。结论 En Vision多标记读板仪作为细胞内钙信号检测仪器,具有快速、可重复性好和灵敏度高等优势,适用于钙信号相关的研究和小分子抑制剂的筛选等领域的工作。
基金Supported by Key Projects in the National Science&Technology Pil-lar Program during the Eleventh Five-Year Plan Period(2006BAD01A1703-1)~~
文摘[Objective] The aim was to study the supporting raising technology of Castanea mollissima Blume molecular marker-assisted selection to reduce the cost and time for chestnut breeding.[Method] To find the best cutting method,10 treatments were designed according to transverse diameter and vertical diameter of the chestnut including different cutting ratios in 1/2,1/3,1/4 and different directions about cross cutting,vertical cutting,bevel cutting and two side-cutting.The germination rate,seedling survival rate and morphological indicators of the treatments were measured;significant difference among them was also analyzed.[Result] 1/3 bevel cutting was the best,which not only could give high germination rate and survival rate,but also could guarantee high quality of nursery stock,which did not grow so differently with the untreated seedlings.[Conclusion] This way can not only reduce too much cost and time for seedling breeding,but also promote the germination of chestnut in advance,which could do much good to production.
文摘Although stone palettes from the Naqada culture are well known, research has been focused mostly on those palettes which are richly decorated. However, streaks and fragments of individual signs are also often found on simple palettes of various shapes. This study shows that the majority of these palettes have quite a number of marks and sign fragments that have been made by different techniques and over a long period of time. This particularly applies to rhomboidal palettes, for most of which there are no ground to infer that they have been used for ritual purposes or for mixing beauty care compositions. It is more likely that they denote an early form of the visual symbol in later periods represented by the head of Ptah＇s staff.
文摘A novel, cheap, disposable and single-use nanoparticles-based nanochannel platform assembled on a flexible substrate for label-free immunosensing is pre- sented. This sensing platform is formed by the dip-coating of a homogeneous and assembled monolayer of carboxylated polystyrene nanospheres （PS, 200 and 500 nm-sized） onto the working area of flexible screen-printed indium tin oxide/polyethylene terephthalate （ITO/PET） electrodes. The spaces between the self-assembled nanospheres generate well-ordered nanochannels, with inter-PS particles distances of around 65 and 24 nm respectively. The formed nanochannels are used for the effective immobilization of antibodies and subsequent protein detection based on the monitoring of [Fe（CN）6]^4- flow through diffusion and the decrease in the differential pulse voltammetric signal upon immunocomplex formation. The obtained sensing system is nanochannel-size dependent and allows human immunoglobulin G （IgG） （chosen as a model analyte） to be detected at levels of 580 ng/mL. The system also exhibits an excellent specificity against other proteins present in real samples and shows good performance with a human urine sample. The developed device represents an integrated and simple biodetection system which overcomes many of the limitations of previously reported nanochannels-based approaches and can be extended in the future to several other immuno and DNA detection systems.
