[Objective] This study was to screen the economic or stable PCR system of rice and detect the generality of the selected system in different molecular markers based on PCR.[Method] With DNA extracted from rice leaves ...[Objective] This study was to screen the economic or stable PCR system of rice and detect the generality of the selected system in different molecular markers based on PCR.[Method] With DNA extracted from rice leaves by CTAB method as the template,PCR system was optimized by L16(45)orthogonal design.[Result] Clear bands were amplified from 16 different combinations,but the amplification effects and yields had difference.The most economic and applicable system was as follows:20 ng DNA template,150 μmol/L dNT...展开更多
At the Plant of Production of Radioisotopes part of Nuclear Center of Mexico （ININ）, weekly radiofarmaceutics of sodium iodohippurate and meta-iodobenzyl-guanidine are prepared, these compounds are known as marked m...At the Plant of Production of Radioisotopes part of Nuclear Center of Mexico （ININ）, weekly radiofarmaceutics of sodium iodohippurate and meta-iodobenzyl-guanidine are prepared, these compounds are known as marked molecules with iodine-131. Currently the processes for the preparation of these compounds are carried out individually and in manual form, in box of gloves, presenting radiological risks. To avoid these risks, this work, shows equipment that is inside a warm cell with the finality of minimizing the radiological risks. Also, this semi-automated equipment has the purpose of to reduce the radiation exposure of personnel involved in the process of marking molecules. The routine industrial production with this equipment starts with the preparation of 9 marked doses of sodium iodohippurate of 2.73 mCi of iodine-131, each of the vials with saline solution of 4.5 mL and the product that containing the marked molecules of 0.5 mL with a percentage of marked of 95.6%. The innovation of this work consists in presenting a new design of equipment, for marked molecules, formed by electrical, mechanical, vacuum systems, and air extraction system.展开更多
Lipid rafts are cholesterol-enriched microdomains and implicated in many essential physiological ac-tivities such as the neurotransmitter release.Many studies have been carried out on the function of rafts inthe plasm...Lipid rafts are cholesterol-enriched microdomains and implicated in many essential physiological ac-tivities such as the neurotransmitter release.Many studies have been carried out on the function of rafts inthe plasma membranes,whereas little is known about the information of such microdomains in subcellularcompartments especially synaptic vesicles(SVs).In the well-studied plasma membranes,several proteinshave been recognized as raft markers,which are used to label or trace rafts.But the raft marker proteinon SVs has not been identified yet.Although some SV proteins,including VAMP and CPE,have beenfound in raft fractions,they cannot be used as markers due to their low abundance in rafts.In this work,we designed several chimera proteins and tested their characteristics for using as SV raft makers.First,we detected whether they located in SVs,and then the chimeras exhibiting the better localization in SVswere further examined for their enrichment in raft using detergent treatment and gradient density floatationanalysis.Our results indicate that one of the chimeric proteins is primarily located in SVs and distributedin raft microdomains,which strongly suggests that it could be served as a raft marker for SVs.展开更多
基金Supported by the Key Project from Department of Science and Technology of Heilongjiang Province(GA06B102-3-4)the Key Project from Heilongjiang Provincial Reclamation General Administration(HNKXIV-01-01-02)~~
文摘[Objective] This study was to screen the economic or stable PCR system of rice and detect the generality of the selected system in different molecular markers based on PCR.[Method] With DNA extracted from rice leaves by CTAB method as the template,PCR system was optimized by L16(45)orthogonal design.[Result] Clear bands were amplified from 16 different combinations,but the amplification effects and yields had difference.The most economic and applicable system was as follows:20 ng DNA template,150 μmol/L dNT...
文摘At the Plant of Production of Radioisotopes part of Nuclear Center of Mexico （ININ）, weekly radiofarmaceutics of sodium iodohippurate and meta-iodobenzyl-guanidine are prepared, these compounds are known as marked molecules with iodine-131. Currently the processes for the preparation of these compounds are carried out individually and in manual form, in box of gloves, presenting radiological risks. To avoid these risks, this work, shows equipment that is inside a warm cell with the finality of minimizing the radiological risks. Also, this semi-automated equipment has the purpose of to reduce the radiation exposure of personnel involved in the process of marking molecules. The routine industrial production with this equipment starts with the preparation of 9 marked doses of sodium iodohippurate of 2.73 mCi of iodine-131, each of the vials with saline solution of 4.5 mL and the product that containing the marked molecules of 0.5 mL with a percentage of marked of 95.6%. The innovation of this work consists in presenting a new design of equipment, for marked molecules, formed by electrical, mechanical, vacuum systems, and air extraction system.
基金Supported by the National Natural Science Foundation of China (No. 30340420442,30330160)the National Basic Research Program of China (No.2004 CB720005)
文摘Lipid rafts are cholesterol-enriched microdomains and implicated in many essential physiological ac-tivities such as the neurotransmitter release.Many studies have been carried out on the function of rafts inthe plasma membranes,whereas little is known about the information of such microdomains in subcellularcompartments especially synaptic vesicles(SVs).In the well-studied plasma membranes,several proteinshave been recognized as raft markers,which are used to label or trace rafts.But the raft marker proteinon SVs has not been identified yet.Although some SV proteins,including VAMP and CPE,have beenfound in raft fractions,they cannot be used as markers due to their low abundance in rafts.In this work,we designed several chimera proteins and tested their characteristics for using as SV raft makers.First,we detected whether they located in SVs,and then the chimeras exhibiting the better localization in SVswere further examined for their enrichment in raft using detergent treatment and gradient density floatationanalysis.Our results indicate that one of the chimeric proteins is primarily located in SVs and distributedin raft microdomains,which strongly suggests that it could be served as a raft marker for SVs.
