The factors that influence the colorimetric gene detection of gold label silver stain and improve the detection signals were studied. The influence of amino DNA probes and thiol DNA modified gold nanoparticles was inv...The factors that influence the colorimetric gene detection of gold label silver stain and improve the detection signals were studied. The influence of amino DNA probes and thiol DNA modified gold nanoparticles was investigated based on a sandwich hybridization system. An increase in amino probe concentration brought about an increase in hybridization signal which reached a threshold corresponding to the saturated concentration of amino probes bounded onto a glass slide surface. Since the steric hindrance effect of nanoparticles was dominant over the influence of a surface area, the bigger gold nanoparticles led to weaker hybridization signals. The hybridization efficiency enhanced significantly with the increase of the thiol DNA modified nanoparticle concentrations. Experimental results showed that 125 μmol/L of the amino DNA probe concentration, 15 nm of the gold nanoparticle diameter, and 4.07 nmol/L of the thiol DNA modified gold nanoparticle concentration were optimal for the detection system. The hybridization signals can be improved remarkably by choosing optimal hybridization conditions.展开更多
The random amplified polymorphic DNA (RAPD) technique was used to amplify DNA fragment, aiming at finding markers linked to the sex determination in Ginkgo biloba L. One thousand and two hundred random decamers had be...The random amplified polymorphic DNA (RAPD) technique was used to amplify DNA fragment, aiming at finding markers linked to the sex determination in Ginkgo biloba L. One thousand and two hundred random decamers had been screened. Of the 8 372 RAPD bands, only a 682 bp RAPD marker generated by a primer (S1478) of random decamer sequence, named S1478-682, was found to be associated with the male plants. This marker was present in all male plants and absent in all female plants. Ginkgo trees collected in both Beijing and Shenyang, China were tested using primer S1478. Positive results were obtained, suggesting S1478-682 could be utilized as a reliable RAPD marker to detect the sexuality of Ginkgo.展开更多
Mercury sphygmomanometer (MSM) is reliable and widely used in clinics and hospitals. The principle of Korotkoff sounds method (KSM) applied in the MSM is also a gold standard to measure blood pressure. Many efforts ha...Mercury sphygmomanometer (MSM) is reliable and widely used in clinics and hospitals. The principle of Korotkoff sounds method (KSM) applied in the MSM is also a gold standard to measure blood pressure. Many efforts have been made attempting to replace MSM, which is criticized for being not healthy and safe. In this research, an electronic blood pressure monitor, named K-sounds electronic sphygmomanometer (KESM), was designed as a substitute to MSM. The three key elements of KSM were proposed for the first time. We used appropriate electronic components to build the KESM which can fulfill the functions related to the three key elements. The KESM, which was easy to operate and free of mercury, followed the same principle as MSM. The same principle guaranteed the comparable accuracy. We took equivalence test and the results showed that the designed KESM was as accurate as the calibrated standard MSM. The designed KESM passed the certifications of SFDA and is qualified in clinics or hospitals for diagnostic purposes.展开更多
The purpose of this article is to present and interpret the case study of the secure electronic transaction （SET） scheme of Internet security, as an illustration of the necessary construction of interoperability sol...The purpose of this article is to present and interpret the case study of the secure electronic transaction （SET） scheme of Internet security, as an illustration of the necessary construction of interoperability solutions for financial services. The interpretation of case data with actor network theory （ANT） provides an illustration of power coalitions among banks to create a de facto standard for transnational electronic payment security on the Internet. After a step of protecting its political interests and well-known technological solutions, the coalition understands that its mission becomes a matter of life and death for its members： Brand-new currency has been invented on the Web and one could develop business and monetary transactions without the banks. This interpretative stage tells us much about the transnational mechanisms of regulation and standardization as well as the ＂translation＂ steps regarding these transnational organizations. However, an additional step has to be added to this interpretative step, a step of framework construction. The aim is to help managers of the transnational firms involved in regulations and standardization to anticipate the evolutions and make relevant decisions. The framework has three distinctive characteristics： the ability to help conception, the ability to help conceive problems ＂ex ante＂, and the ability to facilitate collective conception of strategic maneuvers.展开更多
The aim of the article is to determine the extent to which customer satisfaction＇s in the e-banking in Semnan area had organizational and customer readiness for developing their services. One of the most important se...The aim of the article is to determine the extent to which customer satisfaction＇s in the e-banking in Semnan area had organizational and customer readiness for developing their services. One of the most important sectors that plays key role for grasping developed condition is banking sector. Banks like other institutions also look for augmenting their profits and increasing their strength by expansion. The designing of bank must involve selection of those that have sufficient success potential. Hence, from several factors of success, the following three criteria factors have been： selected-customer satisfied, one dimensional, and product fully functional. These factors offers three question multi criteria group extracted from a 16 norms. Also, three criteria factors have been studied over a period of five years and important criteria for evaluating the norms. The Analytical Hierarchy Process （AHP） has been employed to calculate the success potential of each norm. The empirical data comprises the longitudinal survey of the agricultural banking of the Semnan province. The paper, also, finds that product fully functional is placed as the first priority.展开更多
A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles(PNA/Ag NPs) has been initially proposed for specific recognition of m RNA.Making use of the controlled silver nanoparticles aggrega...A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles(PNA/Ag NPs) has been initially proposed for specific recognition of m RNA.Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA–RNA complex, proto-oncogene c-Myc m RNA detection can be achieved. Moreover, the PNA/Ag NPs platform can undergo color change in response to target c-Myc m RNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes.展开更多
Close-packed Ag pyramidal arrays have been fabricated by using inverted pyramidal pits on Si as a template and used to generate plentiful and homogeneous surface-enhanced Raman scattering （SERS） hot sites. The sharp...Close-packed Ag pyramidal arrays have been fabricated by using inverted pyramidal pits on Si as a template and used to generate plentiful and homogeneous surface-enhanced Raman scattering （SERS） hot sites. The sharp nanotip and the four edges of the Ag pyramid result in strong electromagnetic field enhancement with an average enhancement factor （EF） of 2.84 × 10^7. Moreover, the features of the close-packed Ag pyramidal array can be well controlled, which allows SERS substrates with good reproducibility to be obtained. The relative standard deviation （RSD） was lower than 8.78% both across a single substrate and different batches of substrates.展开更多
文摘The factors that influence the colorimetric gene detection of gold label silver stain and improve the detection signals were studied. The influence of amino DNA probes and thiol DNA modified gold nanoparticles was investigated based on a sandwich hybridization system. An increase in amino probe concentration brought about an increase in hybridization signal which reached a threshold corresponding to the saturated concentration of amino probes bounded onto a glass slide surface. Since the steric hindrance effect of nanoparticles was dominant over the influence of a surface area, the bigger gold nanoparticles led to weaker hybridization signals. The hybridization efficiency enhanced significantly with the increase of the thiol DNA modified nanoparticle concentrations. Experimental results showed that 125 μmol/L of the amino DNA probe concentration, 15 nm of the gold nanoparticle diameter, and 4.07 nmol/L of the thiol DNA modified gold nanoparticle concentration were optimal for the detection system. The hybridization signals can be improved remarkably by choosing optimal hybridization conditions.
文摘The random amplified polymorphic DNA (RAPD) technique was used to amplify DNA fragment, aiming at finding markers linked to the sex determination in Ginkgo biloba L. One thousand and two hundred random decamers had been screened. Of the 8 372 RAPD bands, only a 682 bp RAPD marker generated by a primer (S1478) of random decamer sequence, named S1478-682, was found to be associated with the male plants. This marker was present in all male plants and absent in all female plants. Ginkgo trees collected in both Beijing and Shenyang, China were tested using primer S1478. Positive results were obtained, suggesting S1478-682 could be utilized as a reliable RAPD marker to detect the sexuality of Ginkgo.
基金Supported by the Innovation Fund Project from Ministry of Science and Technology of China (08C26214401239)
文摘Mercury sphygmomanometer (MSM) is reliable and widely used in clinics and hospitals. The principle of Korotkoff sounds method (KSM) applied in the MSM is also a gold standard to measure blood pressure. Many efforts have been made attempting to replace MSM, which is criticized for being not healthy and safe. In this research, an electronic blood pressure monitor, named K-sounds electronic sphygmomanometer (KESM), was designed as a substitute to MSM. The three key elements of KSM were proposed for the first time. We used appropriate electronic components to build the KESM which can fulfill the functions related to the three key elements. The KESM, which was easy to operate and free of mercury, followed the same principle as MSM. The same principle guaranteed the comparable accuracy. We took equivalence test and the results showed that the designed KESM was as accurate as the calibrated standard MSM. The designed KESM passed the certifications of SFDA and is qualified in clinics or hospitals for diagnostic purposes.
文摘The purpose of this article is to present and interpret the case study of the secure electronic transaction （SET） scheme of Internet security, as an illustration of the necessary construction of interoperability solutions for financial services. The interpretation of case data with actor network theory （ANT） provides an illustration of power coalitions among banks to create a de facto standard for transnational electronic payment security on the Internet. After a step of protecting its political interests and well-known technological solutions, the coalition understands that its mission becomes a matter of life and death for its members： Brand-new currency has been invented on the Web and one could develop business and monetary transactions without the banks. This interpretative stage tells us much about the transnational mechanisms of regulation and standardization as well as the ＂translation＂ steps regarding these transnational organizations. However, an additional step has to be added to this interpretative step, a step of framework construction. The aim is to help managers of the transnational firms involved in regulations and standardization to anticipate the evolutions and make relevant decisions. The framework has three distinctive characteristics： the ability to help conception, the ability to help conceive problems ＂ex ante＂, and the ability to facilitate collective conception of strategic maneuvers.
文摘The aim of the article is to determine the extent to which customer satisfaction＇s in the e-banking in Semnan area had organizational and customer readiness for developing their services. One of the most important sectors that plays key role for grasping developed condition is banking sector. Banks like other institutions also look for augmenting their profits and increasing their strength by expansion. The designing of bank must involve selection of those that have sufficient success potential. Hence, from several factors of success, the following three criteria factors have been： selected-customer satisfied, one dimensional, and product fully functional. These factors offers three question multi criteria group extracted from a 16 norms. Also, three criteria factors have been studied over a period of five years and important criteria for evaluating the norms. The Analytical Hierarchy Process （AHP） has been employed to calculate the success potential of each norm. The empirical data comprises the longitudinal survey of the agricultural banking of the Semnan province. The paper, also, finds that product fully functional is placed as the first priority.
基金the National Natural Science Foundation of China (21305058, 21205056, 21075058 and 21503104)Tai-Shan Scholar Research Fund of Shandong Province
文摘A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles(PNA/Ag NPs) has been initially proposed for specific recognition of m RNA.Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA–RNA complex, proto-oncogene c-Myc m RNA detection can be achieved. Moreover, the PNA/Ag NPs platform can undergo color change in response to target c-Myc m RNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes.
文摘Close-packed Ag pyramidal arrays have been fabricated by using inverted pyramidal pits on Si as a template and used to generate plentiful and homogeneous surface-enhanced Raman scattering （SERS） hot sites. The sharp nanotip and the four edges of the Ag pyramid result in strong electromagnetic field enhancement with an average enhancement factor （EF） of 2.84 × 10^7. Moreover, the features of the close-packed Ag pyramidal array can be well controlled, which allows SERS substrates with good reproducibility to be obtained. The relative standard deviation （RSD） was lower than 8.78% both across a single substrate and different batches of substrates.
