AIM： To assesse changes in plasma HBV DNA after TAE in HBV-related HCC and correlate the levels with the pattern of lipiodol accumulation on CT. METHODS： Between April and June 2001, 14 patients with HBV-associated ...AIM： To assesse changes in plasma HBV DNA after TAE in HBV-related HCC and correlate the levels with the pattern of lipiodol accumulation on CT. METHODS： Between April and June 2001, 14 patients with HBV-associated HCC who underwent TAE for inoperable or recurrent tumor were studied. Levels of plasma HBV DNA were measured by real-time quantitative PCR daily for five consecutive days after TAE. More than twofold elevation of circulating HBV DNA was considered as a definite elevation. Abdominal CT was performed 1-2 mo after TAE for the measurement of lipiodol retention. RESULTS： Circulating HBV DNA in 10 out of 13 patients was elevated after TAE, except for one patient whose plasma HBV DNA was undetectable before and after TAE. In group Ⅰ patients （n = 6）, the HBV DNA elevation persisted for more than 2 d, while in group Ⅱ （n = 7）, the HBV DNA elevation only appeared for i d or did not reach a definite elevation. There were no significant differences in age or tumor size between the two groups. Patients in group Ⅰ had significantly better lipiodol retention （79.31±28.79%） on subsequent abdominal CT than group Ⅱ （18.43±10.61%） （P = 0.02）. CONCLUSION： Patients with durable HBV DNA elevation for more than 2 d correlated with good lipiodol retention measured 1 mo later, while others associated with poor lipiodol retention. Thus, circulating HBV DNA may be an early indicator of the success or failure of TAE.展开更多
Objective： To establish the animal model for the chronic pulmonary embolism and do some research on it. Methods： Pulmonary arteriography by DSA were performed in 6 goats and the stent was released in the infer-pulmo...Objective： To establish the animal model for the chronic pulmonary embolism and do some research on it. Methods： Pulmonary arteriography by DSA were performed in 6 goats and the stent was released in the infer-pulmonary arterary. Arterial blood gas analysis and right heart catheterization were done. After 3 days of embolism, pulmonary arteriography were performed again. After the animal was sacrificed, the right and left infer-pulmonary tissues were prepared and pathologically studied. Results： Stents were smoothly released in 6 animals. Compared with that before embolism, the arterial blood gas showed no difference 10 minutes later and 3 days later of the stent released. The pulmonary angiography showed that the right infra pulmonary artery was partly blocked and the blood could still pass by. 3 d of the embolism later, the stent was placed well and the right pulmonary artery was completely blocked. Compared with that before embolism,the right atrial pressure, ventricular pressure and the pulmonary pressure in 10 rain after embolism did not change much （ P 〉 0.05 ）, while 3 d after embolism, the pressures were obviously changed（P 〈 0.05 ）. There was no atelectasis ,pleural effusion in the right infra lobe in the experimental group. The wall of blood capillary among the alve- olar wall were significantly dilated. Hyperemia and edema were also observed. Lots of monocyte infiltrated. Localized exudates appeared in alveolar. Conclusion： By releasing stent in the pulmonary, the chronic pulmonary embolism model could be established in the animal experiment, and it should have great potential value in the following study.展开更多
基金Supported by the grant NSC91-2314-B-195-026 from National Science Council, Taiwan, China
文摘AIM： To assesse changes in plasma HBV DNA after TAE in HBV-related HCC and correlate the levels with the pattern of lipiodol accumulation on CT. METHODS： Between April and June 2001, 14 patients with HBV-associated HCC who underwent TAE for inoperable or recurrent tumor were studied. Levels of plasma HBV DNA were measured by real-time quantitative PCR daily for five consecutive days after TAE. More than twofold elevation of circulating HBV DNA was considered as a definite elevation. Abdominal CT was performed 1-2 mo after TAE for the measurement of lipiodol retention. RESULTS： Circulating HBV DNA in 10 out of 13 patients was elevated after TAE, except for one patient whose plasma HBV DNA was undetectable before and after TAE. In group Ⅰ patients （n = 6）, the HBV DNA elevation persisted for more than 2 d, while in group Ⅱ （n = 7）, the HBV DNA elevation only appeared for i d or did not reach a definite elevation. There were no significant differences in age or tumor size between the two groups. Patients in group Ⅰ had significantly better lipiodol retention （79.31±28.79%） on subsequent abdominal CT than group Ⅱ （18.43±10.61%） （P = 0.02）. CONCLUSION： Patients with durable HBV DNA elevation for more than 2 d correlated with good lipiodol retention measured 1 mo later, while others associated with poor lipiodol retention. Thus, circulating HBV DNA may be an early indicator of the success or failure of TAE.
基金supported financially by the Youth Scholar Foundation of city health administration of shanghai(No044Y02)
文摘Objective： To establish the animal model for the chronic pulmonary embolism and do some research on it. Methods： Pulmonary arteriography by DSA were performed in 6 goats and the stent was released in the infer-pulmonary arterary. Arterial blood gas analysis and right heart catheterization were done. After 3 days of embolism, pulmonary arteriography were performed again. After the animal was sacrificed, the right and left infer-pulmonary tissues were prepared and pathologically studied. Results： Stents were smoothly released in 6 animals. Compared with that before embolism, the arterial blood gas showed no difference 10 minutes later and 3 days later of the stent released. The pulmonary angiography showed that the right infra pulmonary artery was partly blocked and the blood could still pass by. 3 d of the embolism later, the stent was placed well and the right pulmonary artery was completely blocked. Compared with that before embolism,the right atrial pressure, ventricular pressure and the pulmonary pressure in 10 rain after embolism did not change much （ P 〉 0.05 ）, while 3 d after embolism, the pressures were obviously changed（P 〈 0.05 ）. There was no atelectasis ,pleural effusion in the right infra lobe in the experimental group. The wall of blood capillary among the alve- olar wall were significantly dilated. Hyperemia and edema were also observed. Lots of monocyte infiltrated. Localized exudates appeared in alveolar. Conclusion： By releasing stent in the pulmonary, the chronic pulmonary embolism model could be established in the animal experiment, and it should have great potential value in the following study.
