一串红株型突变体AFLP分析

一串红(Salvia splendens)株型突变体BN35-1分枝能力强,不必摘心,株型自然成球。本研究利用AFLP(amplified fragment length polymorphism)分子标记技术对其进行分析。从21对引物组合中筛选出6对多态性高的引物组合对突变体BN35-1、野生型BN35及4个商品种进行亲缘关系分析以确定BN35-1与BN35亲缘关系。另外用21对供试引物组合单独对突变体及原种进行多态性分析以寻找差异条带。结果表明,BN35-1与BN35亲缘关系最近,相似系数为0.986 1,远高于其他商品种、BN35与商品种以及商品种间的相似性。在21对供试引物组合中,12对引物检测出BN35-1与BN35基因组DNA共52处基因座位位点存在差异,相应的特异条带是控制株型突变或与控制株型突变基因相连锁的侯选基因片段,深入研究工作有待进一步进行。

拟南芥株型突变体zpr1的性状特征与基因鉴定分析

对本研究室经T-DNA插入法获得的拟南芥株型突变株系——隐性突变体zpr1植株进行植物学性状调查和遗传分析,并对该突变基因进行鉴定、表达定位和调控元件分析。结果显示:(1)性状分析表明,与野生型拟南芥Ws-2相比,突变体zpr1的茎生叶分枝数量增加,茎生叶分枝发生于拟南芥顶端花序部位;野生型拟南芥茎生叶为披针形,而突变体zpr1没有出现分枝的茎生叶呈倒卵形,出现分枝的茎生叶呈披针型;突变体zpr1的主花序高度、株高、分枝高度和分枝长度都高于野生型,且分枝数多于野生型。(2)利用质粒挽救和反向PCR法(IPCR)确定了ZPR1基因突变发生位置是该基因起始密码子上游426bp处,证明T-DNA插入破坏了ZPR1基因的启动子区域,导致该基因在拟南芥内不能正常表达。(3)基因转录调控区域的顺式作用元件分析发现在ZPR1基因的转录调控区有多个与植物激素相关的调控元件,还有与光周期调节相关的调控元件。(4)亚细胞定位发现,ZPR1基因在所有细胞中的细胞膜中表达,而在部分细胞的细胞膜、细胞质和细胞核中均有表达。研究表明,ZPR1基因的表达对植物株型发育有重要的调控作用,该基因的表达水平受植物激素和光照的调节,最终导致了植物株型的变化。

一串红自封顶型品种内源激素含量特征分析

为了解一串红(Salvia splendens)‘彩铃红’株型突变机理,以‘彩铃红’和其野生型品系‘35’的一级侧枝和扦插苗(一级侧枝为插穗)为材料,通过测定侧芽萌动前后‘彩铃红’和‘35’的茎尖、茎节及根的内源激素含量,分析了两者内源激素含量特征差异。结果表明,在侧芽萌动前和后,‘彩铃红’和‘35’不同组织中生长素、细胞分裂素、脱落酸含量和茎节中生长素与细胞分裂素比值差异较大。侧芽萌动前,‘彩铃红’茎尖和茎节生长素含量均比‘35’低,茎节内细胞分裂素活性成分含量高于‘35’,茎节内脱落酸含量低于‘35’,茎节内生长素与细胞分裂素比值低于‘35’。侧芽萌动后,‘彩铃红’茎尖和茎节生长素含量均比‘35’高,茎尖和根尖细胞分裂素含量高于‘35’,茎节脱落酸含量远低于‘35’,茎节内生长素与细胞分裂素比值高于‘35’。

Identification and Analysis of Mature Plant Type Mutants of M_1 Generation Foxtail Millet Changnong35 Treated with EMS Mutagenesis

[Objective] The aim was to identify the mutants of millet Changnong35 induced by different concentrations of EMS, so as to construct a millet mutant library. [Method] Foxtail millet cultivar Changnong35 which is widely used in agricultural production, was treated with 0.8% and 1.0% EMS; and then seven traits of mutants were investigated analyzed, to classify the mutants into different groups. [Result] 282 mutants in the M1 generation related to plant type were obtained, of which, 100 mutant plants treated with 0.8% EMS can be divided into 10 groups; 182 mutant plants obtained by using 1.0% EMS can be divided into 17 groups. The analysis results of the mature plant type traits of the M1 Generation showed that, plant height, diameter of stem under spike, diameter of the first internode under spike and internode number of the mutants treated with 1.0% EMS were significantly different from those of control, while those of mutants treated with 0.8% EMS did not show significant difference from those of control. [Conclusion] The inducing with 1.0% EMS was more conducive to obtain a large number and different types of mutants from Changnong35.