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BnGID1基因过量表达对甘蓝型油菜矮化突变体NDF-1株高的恢复 被引量:5
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作者 李晓晨 李华鹏 +2 位作者 邓喜 马欣荣 王茂林 《应用与环境生物学报》 CAS CSCD 北大核心 2010年第2期192-196,共5页
在正常株高油菜中克隆了与拟南芥矮化突变相关基因AtGID1同源的BnGID1基因全长cDNA目的片段,将其正向插入表达载体pFGC5941上的CaMV35S启动子下游,构成该基因过量表达载体pFGC5941-BnGID1.经根癌农杆菌介导,导入甘蓝型油菜矮化突变体ND... 在正常株高油菜中克隆了与拟南芥矮化突变相关基因AtGID1同源的BnGID1基因全长cDNA目的片段,将其正向插入表达载体pFGC5941上的CaMV35S启动子下游,构成该基因过量表达载体pFGC5941-BnGID1.经根癌农杆菌介导,导入甘蓝型油菜矮化突变体NDF-1基因组,通过PCR及半定量RT-PCR检测,确定得到抗性转基因植株23株.移至大田生长,观察结果显示,转基因植株明显高于对照矮化突变体NDF-1,平均高出49.05cm.结果表明,BnGID1基因过量表达能够使甘蓝型油菜矮化突变体NDF-1株高得到较大程度的恢复. 展开更多
关键词 甘蓝型油菜 BnGID1基因 矮化突变体 过量表达载体 遗传转化 株高恢复
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Research on Relationship between Recovery Ability after Drought Stress-rewatering and Water Use Efficiency of Winter Wheat Varieties
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作者 黄桂荣 张欣莹 +1 位作者 王雅静 钟秀丽 《Agricultural Science & Technology》 CAS 2017年第12期2236-2240,2245,共6页
The study aims at exploring the possibility of using the recovery ability af- ter drought stress-rewatering at vegetative growth stage as the evaluating index in water use efficiency (WUE) of winter wheat varieties.... The study aims at exploring the possibility of using the recovery ability af- ter drought stress-rewatering at vegetative growth stage as the evaluating index in water use efficiency (WUE) of winter wheat varieties. 'Jing 411 ', 'Jinmai 47' and their 34 near isogenic lines (NILs) were used as test materials. Semi-automatic rainproof shelter and the percolating pools were used for simulating drought treat- ment. After suffering severe drought stress, winter wheat crops were rewatered at early jointing stage. The biomass accumulation after rewatering was determined as recovery ability index. In the meanwhile, plant height in the end of vegetative growth stage was measured, and WUE of varieties/lines was also determined. Thereafter, the differences in recovery ability, plant height and the population WUE, together with the correlation between recovery ability and population WUE were analyzed, respectively. The results showed that there were significant differences in recovery ability among some varieties/lines. The recovery ability was affected by both geno- type and environment, and the interaction existed in these two factors. Significant differences existed in plant height and population WUE among the 34 NILs along with their parents. There was a significantly positive correlation between recovery ability and plant height of varieties/lines. Recovery ability and plant height were very significantly and positively correlated with population yield WUE respectively. The re- sults indicated that recovery ability after drought stress-rewatering could be used as an evaluating index of population WUE under drought condition. 展开更多
关键词 Drought stress-rewatering Near isogenic lines Recover ability Plantheight Water use efficiency
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