基于智能化控制的高效活细胞成像技术

为提升活细胞功能研究的效率与准确性,显微成像平台研发了1款智能化控制精准加样系统。自主研发的加样系统具备远程自动化控制功能,能实现精准的药物定时定点添加。经研究和测试,该系统展现出卓越的通用性,能与常见的光学成像设备搭载使用,并能满足从短期到长期活细胞功能研究的各种实验要求。在活细胞连续成像的过程中,采用智能化与精准化的控制方法,可显著提升实验的准确性、稳定性和效率,尤其是能确保关键数据的完整性。该研究工作有效促进了细胞功能等关键科研领域的深入探索,成功增强了多台光学成像仪器的测试能力和应用价值,同时也推动了创新人才的成长和高标准科研平台的搭建。

烧结配料系统仪表的自动控制应用

介绍烧结配料系统中仪表与电气的自动控制,根据烧结工艺需求能将各种矿按照所需量自动投入,由皮带运到混合机中进行下一步工艺的生产。

印刷行业中的色彩管理

生活需要色彩,因为大自然本身就是丰富多彩的.色彩逼真的建筑效果图、层次分明的卫星遥感图、还原准确的印刷品样张和生动形象的大幅彩照,所有这些让人目眩神迷的事物,都跟色彩密切相关.这些均需要通过扫描仪或数字相机等输入设备获取图像,由显示器的屏幕来观察图像色彩,然后通过一台大幅面彩色喷墨打印机或一台印刷机来实现色彩的还原.

Research of Dynamic Monitoring on Arable Land Quality

In China with such a large population and few land, the problem of arable land is particularly prominent. While the amount of arable land per capita is small, the quality of arable land is declining. Compared with the decreasing arable land, the decline in the quality of arable land is invisible and hard to be detected. But the impact is no less than the amount of arable land reduced, and ~;hanges in the quality of arable land pose a serious threat to ecological environment and socio- economic development. Against the background of high-intensity development and the amount of arable land decreasing, the research of monitoring of arable land quality is of great realistic significance.

柔印粘版九问

一、为什么说要特别重视粘版工作? 一般情况下,粘版机(有人称之为上版机)都是作为柔性版印刷机的配套设备购进的,即使是视频上版机,其原理结构也比柔印机简单得多.

国际食品微生物学制标和实验技术研究进展

国际食品微生物学制标和实验技术研究进展黑龙江省哈尔滨市卫生防疫站（１５００１０）于海川高迎春为确保食品的卫生安全性、防止微生物和有毒化学物质对食品的污染，建国后有关部门即着手制定了食品卫生检验方法，６０年代初期制订了食品卫生微生物及化学检验方法，７０...

Fracture strength evaluation of titanium alloys using modified average stress criterion

Structural integrity procedures were used to demonstrate the fitness for the purpose of engineering components transmitting loads. The prediction of the fracture strength of titanium alloys containing sharp notches through the damage model depends on the un-notched strength and the critical length of the damage zone ahead of the notch. In general, the critical length of the damage zone depends on the material, specimen, and size of the sharp notch. Modifications were made in one of the stress fracture criteria known as the average stress criterion for accurate prediction of notched tensile strength of titanium alloy specimen containing sharp notches. To examine the adequacy of these modifications, fracture data of center-cracked titanium alloys with various thicknesses are considered. The notched （fracture） strength estimates are found to be close to the test results. The modified average stress criterion is very simple to predict the notched tensile strength.

Effect of aging treatment on irradiation-induced segregation of high Mn-Cr steel

To investigate the effect of aging treatment on irradiation-induced segregation of high Mn-Cr steel, specimens for electron-beam irradiation were prepared from the high Mn-Cr austenitic steel which was solution treated at 1 373 K for 1 h and aging treated at 573 K for 1 000 h, respectively. The electron-beam irradiation was performed at 573 K up to doses of 5.4 dpa in a 1 250 kV HVEM and irradiation-induced segregation analyses were carried out by an EDX in a 200 kV FE-TEM. The results show that void formation is not observed in both solution treated and aging treated ones. The amount of Cr segregation at the grain boundary decreases in the aged one; however, that of Mn is not changed in solution treated one.

IMAGE PROFILE AREA CALCULATION BASED ON CIRCULAR SAMPLE MEASUREMENT CALIBRATION

A practical approach of measurement calibration is presented for obtaining the true area of the photographed objects projected in the 2-D image scene. The calibration is performed using three circular samples with given diameters. The process is first to obtain the ratio mm/pixel in two orthogonal directions, and then use the obtained ratios with the total number of pixels scanned within projected area of the object of interest to compute the desired area. Compared the optically measured areas with their corresponding true areas, the results show that the proposed method is quite encouraging and the relevant application also proves the approach adequately accurate.

Comparison of RFFIT Tests with Different Standard Sera and Testing Procedures

The World Health Organization （WHO） standard assay for determining antibody level is the rapid fluorescent focus inhibition test （RFFIT） and is used to determine the degree of immunity after vaccination against rabies. To compare the difference in RFFIT results between the laboratories of The National Institute of Infectious Disease in Japan （NIID） and the Chinese Centre for Disease Control （CCDC） as well the influence of the choice of standard serum （STD） for the detection, the two laboratories detection methods were simultaneously manipulated by RFFIT. The reference serums used in NIID and the WHO standard serum used in CCDC were compared in the same RFFIT detection to determine the titer of four sera samples C1, Sl, S2 and S4 in parallel, and the titers of the detected sera samples were calculated using the standard formula for neutralizing antibody titer. No significant difference was found in RFFIT methods from the two laboratories and the RFFIT testing procedures of the two laboratories have good consistency. However, different titers were obtained with the tentative internal standard serum （TI-STD） produced by adjusting to 2.0 IU of WHO standard serum in NIID and the WHO STD. The titer determined with the TI-STD was higher than that determined with WHO STD, This difference appears to be significant and requires further investigation

Standard-sample bracketing calibration method combined with Mg as an internal standard for silicon isotopic compositions using multi-collector inductively coupled plasma mass spectrometry

Silicon isotope analysis traditionally uses a standard-sample bracketing （SSB） method that relies upon greater instrument stability than can be consistently expected. The following proposed method reduces the level of instrumental stability required for the analysis process and provides a valid solution for high-precision and accurate studies of Si isotopic compositions. Rock samples were dissolved by using alkali fusion and acidification. Silicon isotopes were purified with an ion exchange resin. Interfering peaks for isotopes were separated by using a Nu Plasma 1700 multi-collector inductively coupled plasma mass spectrometry （MS） system in high-resolution mode （M/AM 〉 8000 RP）. Two magnesium isotopes （25Mg and 26Mg） and three silicon isotopes （28Si, 29Si, and 3;Si） were analyzed in the same data collection cycle. Mg isotopes were used as an internal standard to calibrate the mass discrimination effects in MS analysis of Si isotopes in combination with the SSB method in order to reduce the effects of MS interference and instrumental mass dis- crimination on the accuracy of measurements. The conventional SSB method without the Mg internal standard and the proposed SSB method with Mg calibration delivered consistent results within two standard deviations. When Mg was used as an internal standard for calibration, the analysis precision was better than 0.05 %0 amu.

Grading Standards of the Coefficient of Variation in the Electronic Raw Silk Size Testing

In order to shorten the difference between the raw silk size grading standards of the world and that of China, to quicken the step of the electronic raw silk testing process, the distribution of the coefficient of variation (CV50m%) of the raw silk size in the electronic testing and the development of the new standards are studied according to the sampling and grading theory. By the theoretical deduction and the simulating experiments, the distribution of the coefficient of variation of the raw silk size is given, and the grading scheme whose quality index is the coefficient of variation(CV50m%)of the raw silk size and the grading precisions of all grades are proposed. Moreover, the rightness and the feasibility of the grading scheme are testified by the sampling and grading simulation.

Improved oxidation resistance of chemical vapor reaction SiC coating modified with silica for carbon/carbon composites

To protect carbon/carbon (C/C) composites from oxidation, a SiC coating modified with SiO2 was prepared by a complex technology. The inner SiC coating with thickness varying from 150 to 300 μm was initially coated by chemical vapor reaction (CVR): a simple and cheap technique to prepare the SiC coating via siliconizing the substrate that was exposed to the mixed vapor (Si and SiO2) at high temperatures (1 923?2 273 K). Then the as-prepared coating was processed by a dipping and drying procedure with tetraethoxysilane as source materials to form SiO2 to fill the cracks and holes. Oxidation tests show that, after oxidation in air at 1 623 K for 10 h and thermal cycling between 1 623 K and room temperature 5 times, the mass loss of the CVR coated sample is up to 18.21%, while the sample coated with modified coating is only 5.96%, exhibiting an obvious improvement of oxidation and thermal shock resistance of the coating. The mass loss of the modified sample is mainly contributed to the reaction of C/C substrate with oxygen diffusing through the penetrating cracks formed in thermal shock tests.

Creep failure mode and criterion of Xiangjiaba sandstone

Based on the results of conventional triaxial compression tests and triaxial compression creep tests on Xiangjiaba sandstone,the failure modes in short-term tests and creep tests,fractography of sandstone after creep failure,short-term and creep failure criterion are analyzed.In the short-term tests and creep tests,the sandstone samples fail in a mix mode consisting of shear failure in a single main plane and tensile failure.Confining pressure can restrict brittle failure and enhance the ductility of sandstone.In the creep tests,brittle fracture is reduced and plastic deformation can fully be developed compared to the condition of short-term tests.And the shear fracture surfaces are flat and they are covered by small particles as a result of friction.When confining pressure increases,particle size decreases while the degree of friction on shear plane increases.On the tensile failure plane,the tensile trace and direction of tearing could be clearly observed.There are obvious tearing steps on the tensile failure plane and tearing laminated structure on the front edge of tearing fracture.The same criterion can be used for the short-term and creep behavior,and the fitting effect using the MOGI criterion is better than the DRUCKER PRAGER criterion.The cohesion and friction angle calculated by the MOGI criterion are in good accordance with those calculated by the MOHR COULOMB criterion.

A Comparative Study of Flavonoid Water-Ethanol Extraction Process in Planted Trollius Chinensis

With rutin standard sample as the comparison, flavonoid extraction rate as an indicator, UV-2450 ultraviolet visible light spectrophotometer to measure flavonoid content in planted Trollius chinensis through orthogonal experiment, this experiment optimizes process condition of flavonoid in Trollius chinensis through water extraction and ethanol extraction. The result shows that flavonoid extraction rate of Trollius chinensis through ethanol extraction method is obviously higher than water extraction, and the optimal extraction process condition is： ethanol concentration is 75%, extraction time is 90min, extraction times are 3, and fluid material ratio is 20：1.

Gel Microbead Cultivation with a Subenrichment Procedure Can Yield Better Bacterial Cultivability from a Seawater Sample than Standard Plating Method

A gel microbead (GMD) cultivation method was employed to cultivate microorganisms from an amphioxus breeding zone in Qingdao, P. R. China. The culture results were compared with those by standard plating method. In the GMD-based method, the microcolony-forming GMDs were sorted by fluorescence-activated cell sorting (FACS). To further get pure cultures, a subsequent enrichment culture and a streaking purification procedure were conducted on marine R2A medium. Eighty bacterial strains isolated by the GMD-based method were randomly selected for sequencing. These isolates belonged to Alphaproteobacteria (33%), Gammaproteobacteria (44%), Bacteroidetes (11%), Actinobacteria (5%), Firmicutes (5%), Epsilonproteobacteria (1%), and Verrucomicrobia (1%), the last two groups being usually difficult to culture. The 16S rRNA gene sequences revealed a diverse community with 91.1%-100% of the bacterial rRNAs similarities. Thirteen strains were sharing 16S rRNA gene sequence which was less than 97% similar to any other rRNA genes currently deposited in TYP16S database. Seventy isolates derived from the standard plating method fell into 4 different taxonomic groups: Alphaproteobacteria (9%), Gammaproteobacteria (81%), Bacteroidetes (7%) and Firmicutes (3%) with a 16S rRNA gene sequence similarities between 95.8%-100%, in which only 3 strains were sharing 16S rRNA gene sequence of less than 97%. The results indicated that the GMD-based method with subenrichment culture yielded more taxonomic groups and more novel microbial strains, including members of previously rarely cultured groups, when compared with the standard plating method, and that this method markedly improved the bacterial cultivability.

On Normal Criterion of Meromorphic Functions

We proved： Let F be a family of meromorphic functions in a domain D anda α ≠0, b ∈C. If f1（z） - α（f（z））^2 ≠ b, f≠ 0 and the poles of f（z） are of multiplicity ≥ 3 foreach f（z） ∈F, then F is normal in D.

Enabling in the Production-Oriented Approach:Theoretical Principles and Classroom Implementation

Enabling as the second phase of the Production-Oriented Approach(POA)instruction(Wen,2015),plays a vital role in determining the quality of productive performance of English language learners.To explore how to apply theoretical principles of enabling to practice,this paper interprets theoretical principles and reports the practice of the classroom implementation of enabling.The paper proposes a definition for enabling,clarifies the confusion between enabling and scaffolding,and clears up some misunderstandings about enabling.It explains three criteria for effective enabling,namely,alignment,gradualness,and variety,and elaborates on the relations among them.Based on the teaching material of Unit 7 of iEnglish,the paper demonstrates how to design and implement enabling activities under the guidance of these three criteria and evaluates the actual effectiveness of the enabling implemented in the classroom.

Characteristics and Causes of the Preconsolidation Stress of Soils in the Yellow River Delta

Preconsolidation stress （ σP） is the maximum effective stress that a soil has suffered throughout its life. From a geotech- nical point of view, pre-consolidation stress has great importance because it separates elastic and reversible deformations from inelastic and only partially irreversible deformations and marks the starting point of high compressibility. In this study we calculated the preconsolidation stress for 72 undisturbed soil samples from the Yellow River Delta, using the oedometer test and applying the method proposed by Casagrande. The results showed that the over-consolidation ratio （OCR-the ratio of pre-consolidation stress to current natural overburden stress） values of the soil from soil surface to 6m depth varied from 1.72 to 15.34 and the maximum pre- consolidation stress was above 200 kPa. In consequence, the soils of the Yellow River Delta are highly over-consolidated within the upper 6m, the OCR decreasing gradually with depth from 6 to 12m. For samples from deeper than 12m the soils were found to be under-consolidated, which was proved by standard penetration tests （SPTs）. The main causes of this type of consolidation are the mineral composition and the designability of the soil body.

Impact of International Financial Reporting Standards （IFRS） Adoption on Key Financial Ratios＂ The Case of the Czech Republic

This contribution analyzes the impact of new International Financial Reporting Standards （IFRS） reporting rules on financial ratios prepared in the Czech companies. Using a sample of 16 Czech firms, we attempt to measure the scope and size of the differences in the selected set of financial ratios as calculated with data reported according to the traditional Czech accounting standards （CAS） and under the IFRS provisions. Our study discovers that there are important differences resulting from the two reporting formats. Our research comes to a conclusion that translation of Czech statements to IFRS may cause changes in the values of financial indicators without relationship to the real change in the firms＇ value, performance, and stability. Even though the findings were not statistically significant, the indicative results of our measurements disclosed an important fact that the transition to IFRS could cause deterioration of key indicators and thereby could impact on the overall rating of companies. One needs to be cautious with generalization due to the small sample size.