[Objective] The research aimed to provide the genetic basis for the protection, development and utilization of Guizhou local pig breeds. [Method] From 27 pairs of porcine microsatellite primers recommended by Food and...[Objective] The research aimed to provide the genetic basis for the protection, development and utilization of Guizhou local pig breeds. [Method] From 27 pairs of porcine microsatellite primers recommended by Food and Agriculture Organization of the United Nations (FAO) and international Society for Animal Genetics (ISAG), six pairs (S0155, SW240, IGF1, SW951, SW857, SW24) were selected for microsatellite DNA detection of three Guizhou local pig breeds, including Nuogu Pig, Kele Pig and Guanling Pig. Subsequently, their genetic diversities were analyzed. [ Result] The three pig breeds were high polymorphic at the six microsatellite loci (PIC 〉0.5). The Nei's standard genetic distance of them was 0.206 3 -0.481 5. The genetic distance between Nuogu Pig and Kele Pig Was the closest, and that between Nuogu Pig and Guanling Pig was the furthest. [ Conclusion] The three Guizhou local pig breeds are in high genetic diversities. Nuogu Pig is a special type of Kele Pig, an excellent Chinese local pig breed.展开更多
据Heub S 2022年6月15日[Sci Rep,2022,12(1):9991-9991.]报道,瑞士CSEM SA研究人员提出了一种高性价比、高效率的微组织组织学处理方法,促进了球状体和器官组织的组织学处理,使凝胶嵌入的三维(3D)细胞模型具有精确的共面排列,与切片平...据Heub S 2022年6月15日[Sci Rep,2022,12(1):9991-9991.]报道,瑞士CSEM SA研究人员提出了一种高性价比、高效率的微组织组织学处理方法,促进了球状体和器官组织的组织学处理,使凝胶嵌入的三维(3D)细胞模型具有精确的共面排列,与切片平面平行,最大限度地减少了样品材料的损失。展开更多
基金Supported by Key Project of Agriculture in Guizhou Province (NY[2008]3042)~~
文摘[Objective] The research aimed to provide the genetic basis for the protection, development and utilization of Guizhou local pig breeds. [Method] From 27 pairs of porcine microsatellite primers recommended by Food and Agriculture Organization of the United Nations (FAO) and international Society for Animal Genetics (ISAG), six pairs (S0155, SW240, IGF1, SW951, SW857, SW24) were selected for microsatellite DNA detection of three Guizhou local pig breeds, including Nuogu Pig, Kele Pig and Guanling Pig. Subsequently, their genetic diversities were analyzed. [ Result] The three pig breeds were high polymorphic at the six microsatellite loci (PIC 〉0.5). The Nei's standard genetic distance of them was 0.206 3 -0.481 5. The genetic distance between Nuogu Pig and Kele Pig Was the closest, and that between Nuogu Pig and Guanling Pig was the furthest. [ Conclusion] The three Guizhou local pig breeds are in high genetic diversities. Nuogu Pig is a special type of Kele Pig, an excellent Chinese local pig breed.
文摘据Heub S 2022年6月15日[Sci Rep,2022,12(1):9991-9991.]报道,瑞士CSEM SA研究人员提出了一种高性价比、高效率的微组织组织学处理方法,促进了球状体和器官组织的组织学处理,使凝胶嵌入的三维(3D)细胞模型具有精确的共面排列,与切片平面平行,最大限度地减少了样品材料的损失。