[Objective ] The study aimed to construct the AFLP molecular marking system in Mangifera indica. [ Method ] Four varieties of Mangifera indica were used to explore new ways for high-quality DNA, and AFLP analysis of 3...[Objective ] The study aimed to construct the AFLP molecular marking system in Mangifera indica. [ Method ] Four varieties of Mangifera indica were used to explore new ways for high-quality DNA, and AFLP analysis of 31 varieties of Mangifera indica was carried out to detect the varietal genetic diversity. [ Result] 14 pairs of primers with stronger polymorphism, better banding patterns and higher resolution were screened out from 64 pairs of selective amplification primers. Then they were used to analyse the fingerprint of 31 varieties of Mangifera indica, the results showed that the ratio of polymorphic bands amplificated by the 14 pairs of primers reached 97% in 31 varieties of Mangifera.[ Conclusion] It was suggested that AFLP was suitable for detecting the polymorphism of Mangifera indica resources.展开更多
The aim of the work was the study of the effect of NEMP （nanosecond electromagnetic pulses） on steel properties. Steel treatment was performed under the workshop conditions. The mechanical properties and the microst...The aim of the work was the study of the effect of NEMP （nanosecond electromagnetic pulses） on steel properties. Steel treatment was performed under the workshop conditions. The mechanical properties and the microstructure of the experimental samples and samples prepared by usual way were analyzed. The treatment of metal melt with nanosecond electromagnetic pulses at the crystallization stage makes it possible to change mechanical properties of metal and its structure.展开更多
The Tunisian coastal oases constitute a model of very rich plant biodiversity, organized in different floors of cultivation. The date palm is the highest, the second is the diversified fruit trees and the smallest is ...The Tunisian coastal oases constitute a model of very rich plant biodiversity, organized in different floors of cultivation. The date palm is the highest, the second is the diversified fruit trees and the smallest is composed by various other productions. The present paper is the results of a RAA diagnosis and bibliographic analysis of nearly thirty years of work on oases. The aim of this study is to focus on the plant diversity and the traditions in using different products. The survey revealed the existence of nearly 45 local date palm varieties, about 26 fruit trees, 65 vegetables and divers other species. It also raises nearly 30 uses of dates and date palm by-products. This survey shows also that oases inhabitants keep in possessing, for a long time, a knowledge to make ingenious concerning use and conservation of the products and by products of the date palm. This survey has all as much demonstrated the risks from weakening this balance which has long been maintained. Some recommendations to maintain and conserve this system are also presented in this paper.展开更多
The lignite samples collected from Giral lignite field of Barmer basin have been subjected to petrological investigation. The data generated has been discussed to understand the evolution of the paleomires of these li...The lignite samples collected from Giral lignite field of Barmer basin have been subjected to petrological investigation. The data generated has been discussed to understand the evolution of the paleomires of these lignites. The present study reveals that these low rank C coals are chiefly composed of huminite group macerals, mainly telohuminite and detrohuminite, while liptinite and inertinite group macerals occur in subordinate amounts. Not much variation in the maceral composition from Seam-I to Seam-VIII has been observed. Barmer lignites are characterized by a very high GI （〉10） and moderate TPI indicating topogenous mire in the basin which was permanently flooded. The GI and TPI values and the petrography-based facies critical models indicate that these lignites originated mostly under wet forest swamp to clastic marsh having telmatic to limno-telmatic conditions with a moderate rate of subsidence and a very slow fall in ground water table. Further, the GWI and VI values are suggestive of mesotrophic to rheotrophic hydrological conditions having the dominance of herbaceous to marginal aquatic vegetation. There were spells of periodic drowning of peat especially during the formation of Seam-VII. Moderately high concentration of calcium in these lignites along with the presence of framboidal pyrite indicate enhanced sulphate-reducing bacterial activity present in carbonate and sulphate-rich waters in the basin during peat formation.展开更多
Minerals which enter lake-marsh ores composition are very diverse. There are two large classes of ores： ferrous and manganese-ferrous ores. Each class is divided into groups according to its mineral composition. In t...Minerals which enter lake-marsh ores composition are very diverse. There are two large classes of ores： ferrous and manganese-ferrous ores. Each class is divided into groups according to its mineral composition. In this investigation, ferrous ore sample （Chagodostcha） and manganese-ferrous ore samples （Totyma-2 and Mologa-2） were considered. All samples in the initial state are contaminated with a-quartz and Ab-An feldspars. Thus for purification of the samples, direct flotation method with kerosene agent were used. As a result, almost all impurities were removed from the samples. With the X-ray diffraction method, the authors identified following phases： goethite （ferrous ore） and birnessite （manganese-ferrous ores）. Also, the authors used electron probe microanalysis of the original and purified probes and it showed the presence of such impurities as Ca, K, Mg and Ba in birnessite from the manganese-ferrous ores, and AI and Mn impurities in goethite from the ferrous ore. All ore minerals are in a nanocrystalline state （crystallite size is about 10 nm）.展开更多
Objective： Hepatocellular carcinoma （HCC） is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. Thi...Objective： Hepatocellular carcinoma （HCC） is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. This study employed a quantitative proteomic strategy to analyze the differential expression of serum glycoproteins between HCC and normal control serum samples. Methods： Lectin affinity chromatography （LAC） was used to enrich glycoproteins from the serum samples. Quantitative mass spectrometric analysis combined with stable isotope dimethyl labeling and 2D liquid chromatography （LC） separations were performed to examine the differential levels of the detected proteins between HCC and control serum samples. Western blot was used to analyze the differential expression levels of the three serum proteins. Results： A total of 2,280 protein groups were identified in the serum samples from HCC patients by using the 2D LC-MS/MS method. Up to 36 proteins were up-regulated in the HCC serum, whereas 19 proteins were down-regulated. Three differential glycoproteins, namely, fibrinogen gamma chain （FGG）, FOS-like antigen 2 （FOSL2）, and a-l, 6-mannosylglycoprotein 6-^-N-acetylglucosaminyltransferase B （MGATSB） were validated by Western blot. All these three proteins were up-regulated in the HCC serum samples. Conclusion： A quantitative glycoproteomic method was established and proven useful to determine potential novel biomarkers for HCC.展开更多
Significant amounts of free amino acids exist in commercially sold vegetables and fruits. Despite of the fact, only a little information is available about the free amino acid contents in foods. To utilize information...Significant amounts of free amino acids exist in commercially sold vegetables and fruits. Despite of the fact, only a little information is available about the free amino acid contents in foods. To utilize information of free amino acids in food, we have carried out the experiments to quantitate the free amino acids by derivatized with NBD-F （4-fluoro-7-nitrobenzofurazan） and analyzed on reversed-phase UHPLC （ultra-high pressure liquid chromatography） equipped with ultraviolet visible detector. Almost all of food extracts contained free amino acids including GABA （T-amino butyrate）. Contents of free amino acids vary considerably depending upon vegetables and fruits. Principal free amino acids found in vegetables and fruits were asparagine, glutamine, arginine and GABA, which are involved in important metabolic pathways in human. About 140 species of vegetables and fruits were subjected for the data base. All of the plants and fruits we examined exhibit significant amount of free amino acids, those are clearly distinct from data bases obtained after acid hydrolysis treated food samples. Since glutamate and GABA act as excitatory and inhibitory neurotransmitters in CNS, respectively; free amino acids in vegetables and fruits that we eat daily, should be an important source for the cellular metabolic activities.展开更多
Highly precise and reliable determination of heavy metals in soil micro-arthropod tissues remains a challenge because of the small size of the animals and their typical low abundance in metal-contaminated agricultural...Highly precise and reliable determination of heavy metals in soil micro-arthropod tissues remains a challenge because of the small size of the animals and their typical low abundance in metal-contaminated agricultural soils. The present study sought to develop a method for cadmium （Cd） determination in soil micro-arthropods by optimizing the sample digestion procedure, reducing sample weight, modifying sample pre-treatment and validating the methodology with field samples. The optimized digestion conditions comprised a sample mass of 50-150 μg, digestion reagent of nitric acid：hydrogen peroxide （3：1）, digestion temperature of 105 ℃, digestion period of 3 h and digestion volume of 30 μL. Defecation of the standard Collembola Folsomia candida （92 h） and the indigenous Collembola Onychiurus yodai （42 h） and ultrasonic cleaning of F. candida increased the accuracy of Cd determination. The recovery of Cd using the refined procedure was 98.9% and the limits of detection and quantification were 0.002 and 0.008 μg L-1, respectively. The within-batch precision values were 〈 3%. The Cd concentrations in the tissues of the Collembola Isotorna sp. collected from a range of metal-contaminated fields determined by the improved method were consistent with the Cd concentrations in the field soils. The results indicate that the optimized method can be used for more accurate or reliable determination of Cd concentrations in soil micro-arthropod tissues.展开更多
Fast and efficient sample preparation methods are a prerequisite for protein identification in bottom-up proteomics. Here, an innovative microwave irradiation sample preparation method was developed based on an optimi...Fast and efficient sample preparation methods are a prerequisite for protein identification in bottom-up proteomics. Here, an innovative microwave irradiation sample preparation method was developed based on an optimized organic-aqueous solvent system for protein identification. Specifically, protein solutions containing high-concentration acetonitrile were subjected to 5 min microwave irradiation. After cooling down, trypsin was added and the digestion was performed with 30 s microwave irradiation, and the resulting peptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS). A shortened processing time of only 5.5 min is needed with this method(more than 12 h is necessary in the traditional overnight protein sample preparation). Moreover, due to the absence of urea and other chaotropic reagents, the digests can be readily identified by MALDI-TOF MS. When an assessment of this method was performed by digesting a model protein BSA, 69% ± 3% sequence coverage corresponding to 47 ± 3 peptides was obtained, which shows better protein identification than that from the standard overnight protein sample preparation method(51% ± 2% sequence coverage and 23 ± 1 peptides). Another model protein α-casein was used for the analysis of protein phosphorylation with the newly developed method that yielded 4 phosphopeptides with 8 phosphorylation sites, whereas 3 phosphopeptides with 2 phosphorylation sites were obtained from the traditional overnight approach. Moreover, the organic-aqueous microwave irradiation method provides effective digestion for proteins down to fmol.展开更多
Emerging and re-emerging viruses continue to surface all over the world.Some of these viruses have the potential for rapid and global spread with high morbidity and mortality,such as the SARS coronavirus outbreak.It i...Emerging and re-emerging viruses continue to surface all over the world.Some of these viruses have the potential for rapid and global spread with high morbidity and mortality,such as the SARS coronavirus outbreak.It is extremely urgent and important to identify a novel virus near-instantaneously to develop an active preventive and/or control strategy.As a cultureindependent approach,viral metagenomics has been widely used to investigate highly divergent and completely new viruses in humans,animals,and even environmental samples in the past decade.A new model of Koch's postulates,named the metagenomic Koch's postulates,has provided guidance for the study of the pathogenicity of novel viruses.This review explains the viral metagenomics strategy for virus discovery and describes viruses discovered in human feces in the past 10 years using this approach.This review also addresses issues related to the metagenomic Koch's postulates and the challenges for virus discovery in the future.展开更多
基金Supported by Key Projects of Basic Platform of the National Science and Technology (2005DKA21005 )National Natural Science Foundation-funded Projects (30260086)~~
文摘[Objective ] The study aimed to construct the AFLP molecular marking system in Mangifera indica. [ Method ] Four varieties of Mangifera indica were used to explore new ways for high-quality DNA, and AFLP analysis of 31 varieties of Mangifera indica was carried out to detect the varietal genetic diversity. [ Result] 14 pairs of primers with stronger polymorphism, better banding patterns and higher resolution were screened out from 64 pairs of selective amplification primers. Then they were used to analyse the fingerprint of 31 varieties of Mangifera indica, the results showed that the ratio of polymorphic bands amplificated by the 14 pairs of primers reached 97% in 31 varieties of Mangifera.[ Conclusion] It was suggested that AFLP was suitable for detecting the polymorphism of Mangifera indica resources.
文摘The aim of the work was the study of the effect of NEMP （nanosecond electromagnetic pulses） on steel properties. Steel treatment was performed under the workshop conditions. The mechanical properties and the microstructure of the experimental samples and samples prepared by usual way were analyzed. The treatment of metal melt with nanosecond electromagnetic pulses at the crystallization stage makes it possible to change mechanical properties of metal and its structure.
文摘The Tunisian coastal oases constitute a model of very rich plant biodiversity, organized in different floors of cultivation. The date palm is the highest, the second is the diversified fruit trees and the smallest is composed by various other productions. The present paper is the results of a RAA diagnosis and bibliographic analysis of nearly thirty years of work on oases. The aim of this study is to focus on the plant diversity and the traditions in using different products. The survey revealed the existence of nearly 45 local date palm varieties, about 26 fruit trees, 65 vegetables and divers other species. It also raises nearly 30 uses of dates and date palm by-products. This survey shows also that oases inhabitants keep in possessing, for a long time, a knowledge to make ingenious concerning use and conservation of the products and by products of the date palm. This survey has all as much demonstrated the risks from weakening this balance which has long been maintained. Some recommendations to maintain and conserve this system are also presented in this paper.
文摘The lignite samples collected from Giral lignite field of Barmer basin have been subjected to petrological investigation. The data generated has been discussed to understand the evolution of the paleomires of these lignites. The present study reveals that these low rank C coals are chiefly composed of huminite group macerals, mainly telohuminite and detrohuminite, while liptinite and inertinite group macerals occur in subordinate amounts. Not much variation in the maceral composition from Seam-I to Seam-VIII has been observed. Barmer lignites are characterized by a very high GI （〉10） and moderate TPI indicating topogenous mire in the basin which was permanently flooded. The GI and TPI values and the petrography-based facies critical models indicate that these lignites originated mostly under wet forest swamp to clastic marsh having telmatic to limno-telmatic conditions with a moderate rate of subsidence and a very slow fall in ground water table. Further, the GWI and VI values are suggestive of mesotrophic to rheotrophic hydrological conditions having the dominance of herbaceous to marginal aquatic vegetation. There were spells of periodic drowning of peat especially during the formation of Seam-VII. Moderately high concentration of calcium in these lignites along with the presence of framboidal pyrite indicate enhanced sulphate-reducing bacterial activity present in carbonate and sulphate-rich waters in the basin during peat formation.
文摘Minerals which enter lake-marsh ores composition are very diverse. There are two large classes of ores： ferrous and manganese-ferrous ores. Each class is divided into groups according to its mineral composition. In this investigation, ferrous ore sample （Chagodostcha） and manganese-ferrous ore samples （Totyma-2 and Mologa-2） were considered. All samples in the initial state are contaminated with a-quartz and Ab-An feldspars. Thus for purification of the samples, direct flotation method with kerosene agent were used. As a result, almost all impurities were removed from the samples. With the X-ray diffraction method, the authors identified following phases： goethite （ferrous ore） and birnessite （manganese-ferrous ores）. Also, the authors used electron probe microanalysis of the original and purified probes and it showed the presence of such impurities as Ca, K, Mg and Ba in birnessite from the manganese-ferrous ores, and AI and Mn impurities in goethite from the ferrous ore. All ore minerals are in a nanocrystalline state （crystallite size is about 10 nm）.
基金supported by grants from the National Natural Science Foundation of China(Grant No.21205088)973 Project(Grant No.2011CB933100)+2 种基金National Science Fund for Distinguished Young Scholars(Grant No.81125019)Doctoral Research Fund from the Ministry of Education of China(Grant No.20121202120001)sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry
文摘Objective： Hepatocellular carcinoma （HCC） is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. This study employed a quantitative proteomic strategy to analyze the differential expression of serum glycoproteins between HCC and normal control serum samples. Methods： Lectin affinity chromatography （LAC） was used to enrich glycoproteins from the serum samples. Quantitative mass spectrometric analysis combined with stable isotope dimethyl labeling and 2D liquid chromatography （LC） separations were performed to examine the differential levels of the detected proteins between HCC and control serum samples. Western blot was used to analyze the differential expression levels of the three serum proteins. Results： A total of 2,280 protein groups were identified in the serum samples from HCC patients by using the 2D LC-MS/MS method. Up to 36 proteins were up-regulated in the HCC serum, whereas 19 proteins were down-regulated. Three differential glycoproteins, namely, fibrinogen gamma chain （FGG）, FOS-like antigen 2 （FOSL2）, and a-l, 6-mannosylglycoprotein 6-^-N-acetylglucosaminyltransferase B （MGATSB） were validated by Western blot. All these three proteins were up-regulated in the HCC serum samples. Conclusion： A quantitative glycoproteomic method was established and proven useful to determine potential novel biomarkers for HCC.
文摘Significant amounts of free amino acids exist in commercially sold vegetables and fruits. Despite of the fact, only a little information is available about the free amino acid contents in foods. To utilize information of free amino acids in food, we have carried out the experiments to quantitate the free amino acids by derivatized with NBD-F （4-fluoro-7-nitrobenzofurazan） and analyzed on reversed-phase UHPLC （ultra-high pressure liquid chromatography） equipped with ultraviolet visible detector. Almost all of food extracts contained free amino acids including GABA （T-amino butyrate）. Contents of free amino acids vary considerably depending upon vegetables and fruits. Principal free amino acids found in vegetables and fruits were asparagine, glutamine, arginine and GABA, which are involved in important metabolic pathways in human. About 140 species of vegetables and fruits were subjected for the data base. All of the plants and fruits we examined exhibit significant amount of free amino acids, those are clearly distinct from data bases obtained after acid hydrolysis treated food samples. Since glutamate and GABA act as excitatory and inhibitory neurotransmitters in CNS, respectively; free amino acids in vegetables and fruits that we eat daily, should be an important source for the cellular metabolic activities.
基金supported by the National High Technology Research and Development Program of China (No. 2012AA101402-2)the National Natural Science Foundation of China (Nos. 41271264, 41325003)
文摘Highly precise and reliable determination of heavy metals in soil micro-arthropod tissues remains a challenge because of the small size of the animals and their typical low abundance in metal-contaminated agricultural soils. The present study sought to develop a method for cadmium （Cd） determination in soil micro-arthropods by optimizing the sample digestion procedure, reducing sample weight, modifying sample pre-treatment and validating the methodology with field samples. The optimized digestion conditions comprised a sample mass of 50-150 μg, digestion reagent of nitric acid：hydrogen peroxide （3：1）, digestion temperature of 105 ℃, digestion period of 3 h and digestion volume of 30 μL. Defecation of the standard Collembola Folsomia candida （92 h） and the indigenous Collembola Onychiurus yodai （42 h） and ultrasonic cleaning of F. candida increased the accuracy of Cd determination. The recovery of Cd using the refined procedure was 98.9% and the limits of detection and quantification were 0.002 and 0.008 μg L-1, respectively. The within-batch precision values were 〈 3%. The Cd concentrations in the tissues of the Collembola Isotorna sp. collected from a range of metal-contaminated fields determined by the improved method were consistent with the Cd concentrations in the field soils. The results indicate that the optimized method can be used for more accurate or reliable determination of Cd concentrations in soil micro-arthropod tissues.
基金supported by the National Basic Research Program of China(2012CB910604,2013CB911202)the National Natural Science Foundation of China(21190043)the Innovation Method Fund of China(2012IM030900)
文摘Fast and efficient sample preparation methods are a prerequisite for protein identification in bottom-up proteomics. Here, an innovative microwave irradiation sample preparation method was developed based on an optimized organic-aqueous solvent system for protein identification. Specifically, protein solutions containing high-concentration acetonitrile were subjected to 5 min microwave irradiation. After cooling down, trypsin was added and the digestion was performed with 30 s microwave irradiation, and the resulting peptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS). A shortened processing time of only 5.5 min is needed with this method(more than 12 h is necessary in the traditional overnight protein sample preparation). Moreover, due to the absence of urea and other chaotropic reagents, the digests can be readily identified by MALDI-TOF MS. When an assessment of this method was performed by digesting a model protein BSA, 69% ± 3% sequence coverage corresponding to 47 ± 3 peptides was obtained, which shows better protein identification than that from the standard overnight protein sample preparation method(51% ± 2% sequence coverage and 23 ± 1 peptides). Another model protein α-casein was used for the analysis of protein phosphorylation with the newly developed method that yielded 4 phosphopeptides with 8 phosphorylation sites, whereas 3 phosphopeptides with 2 phosphorylation sites were obtained from the traditional overnight approach. Moreover, the organic-aqueous microwave irradiation method provides effective digestion for proteins down to fmol.
文摘Emerging and re-emerging viruses continue to surface all over the world.Some of these viruses have the potential for rapid and global spread with high morbidity and mortality,such as the SARS coronavirus outbreak.It is extremely urgent and important to identify a novel virus near-instantaneously to develop an active preventive and/or control strategy.As a cultureindependent approach,viral metagenomics has been widely used to investigate highly divergent and completely new viruses in humans,animals,and even environmental samples in the past decade.A new model of Koch's postulates,named the metagenomic Koch's postulates,has provided guidance for the study of the pathogenicity of novel viruses.This review explains the viral metagenomics strategy for virus discovery and describes viruses discovered in human feces in the past 10 years using this approach.This review also addresses issues related to the metagenomic Koch's postulates and the challenges for virus discovery in the future.
