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Impacts of Environmental Factors on the Structure Characteristics of Avian Community in Shanghai Woodlots in Spring 被引量:31
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作者 葛振鸣 王天厚 +2 位作者 施文彧 周立晨 薛文杰 《Zoological Research》 CAS CSCD 北大核心 2005年第1期17-24,共8页
From March to May, 2004, we selected 8 typical parks or green lands in Shanghai downtown as the study sites to conduct a ornithological research. During this study, total 55 species of birds were recorded, which inclu... From March to May, 2004, we selected 8 typical parks or green lands in Shanghai downtown as the study sites to conduct a ornithological research. During this study, total 55 species of birds were recorded, which included 31 resident and 24 migratory species. With statistics methodology, we analyzed the avian communities with 10 major environmental factors covering the park area, water percentage, vegetation species, canopy cover, shrub cover, grass cover, hill number, location condition, human quantity, path width, the study results indicated that: (1) the following 7 environmental factors were important to impact the structures of the avian community, they were park area, vegetation species, shrub coverage, grass coverage, hill number, location condition of the parks and path width; (2) the avian community of theses urban parks tended to deteriorate in the bird number and diversity; the man-made lake which was currently promoted in the park designing and planning process would not be good to attract the birds. 展开更多
关键词 Urban avian community Bird diversity Key environmental factor Shanghai park
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面向术前皮瓣设计:三维创面的形态学展开与分析 被引量:2
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作者 纪小刚 栾宇豪 +2 位作者 张溪溪 张建安 胡海涛 《计算机辅助设计与图形学学报》 EI CSCD 北大核心 2020年第6期989-996,共8页
临床上由于创伤所致的皮肤等软组织缺损创面形状常不规则,目前仅采用样布描记皮瓣形状进行术前设计,其方法较为粗糙,所切取皮瓣往往无法与创面相契合,且影响术后外观.为解决这类问题,提出一种基于层次值的皮肤创面形态学展开方法.首先... 临床上由于创伤所致的皮肤等软组织缺损创面形状常不规则,目前仅采用样布描记皮瓣形状进行术前设计,其方法较为粗糙,所切取皮瓣往往无法与创面相契合,且影响术后外观.为解决这类问题,提出一种基于层次值的皮肤创面形态学展开方法.首先对皮肤创面进行层次划分,分层拓扑映射至平面.然后结合力学方法模拟皮肤的应力和应变,分层优化皮肤创面的展开形状,实现局部创面和创面整体的并行优化.最后对生成的皮肤展开轮廓进行光顺,得到面向术前皮瓣设计的指导方案.通过对十几例不同部位的皮肤创面进行展开与分析,结果表明,文中方法获得的展开结果能更好地与皮肤创面相契合,展开后的面积和边长平均偏差值缩小至10%以内. 展开更多
关键词 形态学展开 样布描记 层次值 拓扑映射
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旋毛虫成囊前期幼虫收集方法的研究 被引量:10
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作者 张荣光 王中全 尹清源 《河南预防医学杂志》 1998年第4期192-193,228,共3页
为了分析旋毛虫成囊前期幼虫(PEL)抗原以寻找更有效的旋毛虫病诊断方法,本实验国内首次对PEL的收集方法进行了研究。在贝氏法原理的基础上,分别采用了160目、200目、240目和280目4种分样筛以及布袋收集PEL,... 为了分析旋毛虫成囊前期幼虫(PEL)抗原以寻找更有效的旋毛虫病诊断方法,本实验国内首次对PEL的收集方法进行了研究。在贝氏法原理的基础上,分别采用了160目、200目、240目和280目4种分样筛以及布袋收集PEL,并观测了PEL的数量和纯度。结果表明:在4种分样筛和各种收集时间中,用240目分样筛收集3h为比较理想的实验条件,在此条件下能够得到大量纯度较高的PEL虫体;另外,用棉布袋比用分样筛的收集效果更好。在收集2.5h时用240目分样筛得到的虫体数量比用棉布袋多(p<0.05),但纯度较低;在收集3h以后,用这两种方法得到的虫体数量相近(P>0.05)。 展开更多
关键词 旋毛虫 成囊前期幼虫 袋法 收集
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Distribution pattern of rare plants along riparian zone in Shennongjia Area 被引量:4
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作者 江明喜 邓红兵 蔡庆华 《Journal of Forestry Research》 SCIE CAS CSCD 2002年第1期25-27,83,共3页
Due to the importance of riparian zone in maintaining and protecting regional biodiversity, increasingly more ecologists paid their attentions to riparian zone and had been aware of the important effects of riparian z... Due to the importance of riparian zone in maintaining and protecting regional biodiversity, increasingly more ecologists paid their attentions to riparian zone and had been aware of the important effects of riparian zone in basic study and practical management. In this study, 42 sampling belts (10 m?00 m) parallel to the bank of Xiangxi River at different elevations in Shennongjia Area were selected to investigate the riparian vegetation and rare plants. 14 species of rare plants were found distributing in riparian zone, accounting for 42.4% of the total rare plant species in Shennongjia Area. The main distribution range of the 14 rare plant species was the evergreen and deciduous mixed broadleaved forest at elevation of 1200-1800 m, where, species diversity of plant community was the maximum at the moderate elevation. The analysis of TWINSPAN divided the 14 rare species into 3 groups against the elevation, namely low elevation species group, moderate elevation species group, and high elevation species group. The analysis of DCA ordination showed similar results to that of TWINSPAN. In the paper, the authors discussed the reasons forming the distribution pattern of rare plant species, and pointed out that the important function of riparian zone on rare plant species protection. 展开更多
关键词 Xiangxi River Rare plant Riparian zone Distribution pattern BIODIVERSITY
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Genetic Diversity of Hordeum bogdanii Wilensky Native to Xinjiang, China, Based on STS-PCR Markers 被引量:3
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作者 郭红 魏育明 +1 位作者 陈放 郑有良 《Acta Botanica Sinica》 CSCD 2002年第11期1327-1332,共6页
The genetic diversity among 32 accessions of Hordeum bogdanii Wilensky native to Xinjiang, China, was evaluated by 22 STS_PCR primer sets derived from RFLP clones of the wheat ( Triticum aestivum L.) or barley ... The genetic diversity among 32 accessions of Hordeum bogdanii Wilensky native to Xinjiang, China, was evaluated by 22 STS_PCR primer sets derived from RFLP clones of the wheat ( Triticum aestivum L.) or barley ( Hordeum vulgare L.) mapping. Out of the 22 STS_PCR markers, only three markers gave products which did not generate polymorphic bands upon digestion with Hin fⅠ, Hha Ⅰ, Hae Ⅲ and Rsa Ⅰ, while 19 out of 22 markers (86.4%) and 46 out of 88 marker/enzyme combinations (52.3%) revealed polymorphisms. Among the 32 H. bogdanii accessions, a total of 315 bands were observed in 88 STS_PCR marker/enzyme combinations, with 3.6 bands each. One hundred and twenty_three out of 315 bands (39.0%) were polymorphic, among which 1 to 6 polymorphic bands were generated by each polymorphic marker/enzyme combination. The STS_PCR_based genetic diversity index ( GD ) among 32 H. bogdanii accessions ranged between 0.078 to 0.352, with a mean of 0.198. Based on the GD matrix, a dendrogram showing the genetic relationships between accessions was constructed using the unweighted pair_group method with arithmetic average (UPGMA). Results showed that all 32 accessions could be distinguished by STS_PCR markers. The accessions originated from the same region were distributed within different groups or subgroups. This study indicates that the genetic diversity of H. bogdanii is not closely correlated with the geographical distribution. 展开更多
关键词 Hordeum bogdanii STS_PCR marker genetic diversity genetic relationship
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Widely Tunable Sampled-Grating DBR Laser
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作者 阚强 赵玲娟 +4 位作者 张靖 周帆 王宝军 王鲁峰 王圩 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2005年第5期853-856,共4页
The 3-section SG-DBR tunable laser is fabricate d using an ion implantation quantum-well intermixing process.The over 30nm discontinuous tuning range is achieved with the SMRS greater than 30dB.
关键词 sampled-grating distributed Bragg reflector laser comb-like spectrum wavelength tuning
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Distributed Sampling Measurement Model in a Large-Scale High-Speed IP Networks 被引量:1
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作者 龚俭 程光 《Journal of Southeast University(English Edition)》 EI CAS 2002年第1期40-45,共6页
The distributed passive measurement is an important technology for networkbehavior research. To achieve a consistent measurement, the same packets should be sampled atdistributed measurement points. And in order to es... The distributed passive measurement is an important technology for networkbehavior research. To achieve a consistent measurement, the same packets should be sampled atdistributed measurement points. And in order to estimate the character of traffic statistics, thetraffic sample should be random in statistics. A distributed samplingmask measurement model isintroduced to tackle the difficulty of measuring the full trace of high-speed networks. The keypoint of the model is to choose some bits that are suitable to be sampling mask. In the paper, thebit entropy and bit flow entropy of IP packet headers in CERNET backbone are analyzed, and we findthat the 16 bits of identification field in IP packet header are fit to the matching field ofsampling mask. Measurement traffic also can be used to analyze the statistical character ofmeasurement sample and the randomicity of the model. At the same time the experiment resultsindicate that the model has a good sampling performance. 展开更多
关键词 sampling measurement bit entropy matching field identification field
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Calculation and Analysis of Near Field of Airborne Short Wave Antenna
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作者 薛正辉 高本庆 +2 位作者 刘瑞祥 吕昕 杨力 《Journal of Beijing Institute of Technology》 EI CAS 1998年第3期286-292,共7页
Aim To calculate and analyze the near field distribution of ariborne short wave antenna. Methods B-spline method was used to get the mathermatital model of a Boeing 707320Baircraft and simulate its short wave antenna ... Aim To calculate and analyze the near field distribution of ariborne short wave antenna. Methods B-spline method was used to get the mathermatital model of a Boeing 707320Baircraft and simulate its short wave antenna . FDTD (finite-difference time-domain) method are ed tO complete the calculation and analysis. Results The near field distributions on aircraft's surface were obtained, the curve and gray figures of the normalized near field value were shown. Conclusion These modeling and calculating methods can provide data foraircraft's EMC analysis and design. 展开更多
关键词 airborne short antenna near field distribution FDTD method B-SPLINE
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DESCRIPTIONS OF THE CYLINDRICAL HELIX WITH NURBS
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作者 孙克豪 乔新 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 1997年第2期190-197,共8页
The representation of a cylindrical helix by Non-Uniform Rational B-Spline (NURBS) curves is presented in this paper. A method is proposed to assess the influences produced by different ways to determine the control v... The representation of a cylindrical helix by Non-Uniform Rational B-Spline (NURBS) curves is presented in this paper. A method is proposed to assess the influences produced by different ways to determine the control ver-texes positions of helix. The error distribution cases between the helix approximated by NURBS curves and the original theoretical one are also analyzed. Meanwhile a computational method that guarantees the precision requirements is presented. 展开更多
关键词 helixes CURVES non-uniform rational B-spline (NURBS) error distribution
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缺面少料时代——从时尚说起
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《中国科技信息》 2004年第6期12-13,共2页
目前,很多中国设计师认识到,布料在服装的设计中往往成为这件作品成功与否的重要因素。国内虽有服装设计、面料设计的行业区分,但是许多面料企业由于技术和观念等各种原因,使得面料设计还停留在纹样设计或者纤维设计上,很少有企业... 目前,很多中国设计师认识到,布料在服装的设计中往往成为这件作品成功与否的重要因素。国内虽有服装设计、面料设计的行业区分,但是许多面料企业由于技术和观念等各种原因,使得面料设计还停留在纹样设计或者纤维设计上,很少有企业能够将面料的织物结构开发与纹样设计有机地结合。服装设计师想要得到可心的面料往往要大费周折,而购买进口面料已经成为一部分设计师取得眩目的视觉效果和完美质感的“捷径”。 展开更多
关键词 时装发 服装设计 服装面料 样布
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Subtree, TASS and an Analysis of the Genus Caragana
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作者 张明理 Pauline Y. LADIGES Gareth NELSON 《Acta Botanica Sinica》 CSCD 2002年第10期1213-1218,共6页
Subtree analysis and three area satements (TASS) procedure are used to deal with the area relationship in historical biogeography. On the basis of the taxon cladogram, the procedure could identify and eliminate the ... Subtree analysis and three area satements (TASS) procedure are used to deal with the area relationship in historical biogeography. On the basis of the taxon cladogram, the procedure could identify and eliminate the paralogy node and determine the informative subtree. The area relationships are generally illustrated in several subtrees and the consensus tree. The distribution pattern of the genus Caragana comprising about 72 species and occurring in 13 areas, was analyzed by using subtree method and TASS procedure in this study. The results showed seven subtrees representing the area relationships of section and series of the genus, and the consensus tree provided the 13 area relationships. These results are congruent with our former result using component analysis for Caragana. 展开更多
关键词 SUBTREE three area satements (TASS) CARAGANA distribution pattern
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Analyzing Undersampled Signals Using High Order Ambiguity Function Algorithm
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作者 陶然 单涛 +1 位作者 周思永 王越 《Journal of Beijing Institute of Technology》 EI CAS 1999年第2期64-69,共6页
Aim To find an effective and fast algorithm to analyze undersampled signals. Methods\ The advantage of high order ambiguity function(HAF) algorithm is that it can analyze polynomial phase signals by phase rank reduct... Aim To find an effective and fast algorithm to analyze undersampled signals. Methods\ The advantage of high order ambiguity function(HAF) algorithm is that it can analyze polynomial phase signals by phase rank reduction. In this paper, it was first used to analyze the parameters of undersampled signals. When some conditions are satisfied, the problem of frequency confusion can be solved. Results and Conclusion\ As an example, we analyze undersampled linear frequency modulated signal. The simulation results verify the effectiveness of HAF algorithm. Compared with time frequency distribution, HAF algorithm reduces computation burden to a great extent, needs weak boundary conditions and doesn't have boundary effect. 展开更多
关键词 undersampled signal frequency estimation time frequency distribution
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Biogeography of the leafhopper subfamily Stegelytrinae(Hemiptera:Cicadellidae),based on a cluster analysis of geographical distribution in areas of endemism combined with phylogeny of the subfamily
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作者 魏琮 程若琳 张雅林 《Entomotaxonomia》 CSCD 北大核心 2012年第4期575-586,共12页
Biogeography of the leaflaopper subfamily Stegelytrinae Baker is studied based on an analysis of geographical distribution of this subfamily worldwide using a cluster analysis of the zoological distribution of areas o... Biogeography of the leaflaopper subfamily Stegelytrinae Baker is studied based on an analysis of geographical distribution of this subfamily worldwide using a cluster analysis of the zoological distribution of areas of endemism as well as the phylogeny of representatives of this subfamily. Results show that the Stegelytrinae mainly occur in the Oriental Region and in the Mediterranean area of the Palaearctic Region, and this extends to the east side of both Wallaee's and Weber's lines. Eleven areas of endemism of this subfamily are recognized. The proportions of endemic taxa in different areas of endemism are generally very high in comparison with other leaflaopper groups, but distinct differences could be found among the different areas of endemism of Stegelytrinae. This subfamily is most intensively diversified in the Indochina Peninsula (INCN). This is the stegelytrine distribution center, having the highest biodiversity at both genetic and species levels. The dendrogram of endemic areas of Stegelytrinae constructed using cluster analysis of the zoological distribution of Stegelytrinae at generic level shows the endemic areas of Stegelytrinae can be divided into 4 large groups. Relationships among different endemic areas of Stegelytrinae correspond largely to the geologic history of related areas, which indicates that the evolution and vicariance of this subfamily have been closely related to the history of continental drift and climate changes. It is deduced that the presumed monophyletic Stegelytrinae originated in the Oriental Region after North America had separated from Eurasia; this is the case in the monophyletic genera group which is supported by the lateral frontal sutures extending dorsally well beyond the corresponding ocellus. In addition, two expanding traces of the Stegelytrinae are presumed, which remain plausible explanations for the dispersal of Stegelytrinae: (1) New Guinea (and probably (+ Australia)) - Kalimantan - Sumatra - Malay Peninsula - Indochina Peninsula - Central and Southern China - (Southwestern China + Nepal + Northeastern India) - (Northwestern India + Eastern Afghanistan); and (2) Indochina Peninsula- Central and Southern China- (Western Asia + Mediterranean Sea coastal area). 展开更多
关键词 distribution biodiversity origin historical biogeography
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Pedodiversity: A Case Study Based on 1:1 Million Scale SOTER of Shandong Province, China 被引量:12
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作者 TANMANZHI ZHANGXUELEI +2 位作者 CHENJIE YANWUJIU YANGYUJIAN 《Pedosphere》 SCIE CAS CSCD 2003年第3期219-226,共8页
The diversity of soils derived from different parent materials and developed on different terrains inShandong Province, China, was analyzed with the theory of pedodiversity using a 1:1000000 scale soilsand terrain dig... The diversity of soils derived from different parent materials and developed on different terrains inShandong Province, China, was analyzed with the theory of pedodiversity using a 1:1000000 scale soilsand terrain digital database (SOTER) of Shandong Province in a geographical information system (GIS)environment. Diversity index of soils derived from different parent materials followed the sequence of igneousrock > sedimentary rock > metamorphic rock > unconsolidated material. Diversity index of soils developedon different terrains followed the sequence of plain > medium-gradient mountain > medium-gradient hill> valley floor > medium-gradient escarpment zone > depression > high-gradient mountain. Abundancedistributions of these soil groups matched the logarithmic normal distributions. 展开更多
关键词 abundance distribution PEDODIVERSITY shandong province SOTER
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Study on the Thermal Insulation Performance of a New Seabed Sediment Fidelity Sampler Based on ANSYS 被引量:1
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作者 LIU Guoying LIU Guijie YUAN Weijie 《Journal of Ocean University of China》 SCIE CAS 2009年第3期277-282,共6页
A new seabed sediment fidelity sampler was developed and its thermal insulation performance was studied and analyzed. The temperature distribution simulation indicated that the sample quality could be insured by using... A new seabed sediment fidelity sampler was developed and its thermal insulation performance was studied and analyzed. The temperature distribution simulation indicated that the sample quality could be insured by using this new sampler. Based on ANSYS10, the temperature finite element model of the sample cylinder was established. According to the law of conservation of energy, the unsteady heat transmit equation of the sampler under solid-liquid coupling condition was derived, then the mathematical model calculation was carried out by using a mixed finite-element finite-difference method, and two thermal insulation methods were used. The simulation was carried out by using the thickness of the thermal insulation layer and heat conductivity as the variable parameters and the temperature distribution of the sampler and related influencing factors were obtained. Optimization analysis was conducted using the simulation data and related parameters and the magnitude ranges of the parameters were obtained that could meet the design temperature requirements. The experimental data and simulation results indicated that the results were in good agreement with the realities, and this sampler might be of value for seabed sediment sampler design and manufacture. 展开更多
关键词 sea bed sediment SAMPLER ANSYS thermal insulation method temperature field
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Spatial Distribution and Risk Assessment of Heavy Metals in Paddy Soils of Yongshuyu Irrigation Area from Songhua River Basin,Northeast China 被引量:9
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作者 CUI Zhengwu WANG Yang +3 位作者 ZHAO Na YU Rui XU Guanghui YU Yong 《Chinese Geographical Science》 SCIE CSCD 2018年第5期797-809,共13页
There is an increasing concern for potentially hazardous metals pollution, which can threaten crops production and human health. In this study, the spatial distribution and environmental risks of eight heavy metals in... There is an increasing concern for potentially hazardous metals pollution, which can threaten crops production and human health. In this study, the spatial distribution and environmental risks of eight heavy metals in surface soil samples collected from the paddy fields in Yongshuyu irrigation area, Northeast China were investigated. The mean concentrations of Pb, Cr, Cu, Ni, Zn, Cd, Hg and As were 34.6 ± 4.67, 82.8 ± 9.51, 17.3 ± 4.09, 21.2 ± 12.0, 88.6 ± 17.9, 0.18 ± 0.15, 0.22 ± 0.07 and 8.77 ± 2.47 mg/kg, respectively, which were slightly higher than their corresponding background values of Jilin Province, indicating enrichment of these metals in the paddy soils, especially for Ni, Cd and Hg. The spatial distribution of heavy metals was closely correlated with local anthropogenic activities, such as agricultural production, mining and transportation. The hot-spot areas of As and Cd were mainly concentrated in the up-midstream where were associated with agricultural activities. Cr and Cu showed similar spatial distributions with hot-spot areas distributed the whole irrigation area uniformly. Ni was mainly distributed in the downstream where Ni quarries concentrated, while the spatial distribution patterns of Hg was mainly located in the upstream and downstream where the soil was significantly influenced by irrigation and coal mining emission. The spatial distributions of Pb and Zn were mainly concentrated along the highway side. The pollution levels of Yongshuyu irrigation area were estimated through index of geo-accumulation(Igeo), Nemerow integrated pollution index(NIPI) and potential ecological risk index(PERI). The results showed that Cd and Hg were the main pollutants in the study area. Health risk assessment results indicated that children were in higher non-carcinogenic and carcinogenic risks than adults with the carcinogenic metal of As. Ingestion was the main exposure pathway to non-carcinogenic and carcinogenic risk for both adults and children. Principal component analysis(PCA) indicated that Cr and Cu were mainly from parent materials, while Cd and As were mainly affected by agricultural activities. Pb and Zn were controlled by traffic activities, and the accumulations of Ni and Hg were associated with mining activities. This study would be valuable for preventing heavy metals inputs and safety in rice production of the Songhua river basin. 展开更多
关键词 spatial distribution heavy metals paddy soil risk assessment source identification Songhua River Basin
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Convergence Rate of E·B Estimation for Location Parameter Function of One-side Truncated Family Under NA Samples 被引量:2
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作者 凌能祥 《Chinese Quarterly Journal of Mathematics》 CSCD 2003年第4期400-405,共6页
In this paper, we construct the E·B estimation for parameter function of one-side truncated distribution under NA samples. Also, we obtain its convergence rate at O(n-q), where q is approaching 1/2.
关键词 NA samples E.B estimation convergence rate
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Spatial distribution of mountainous regions and classifications of economic development in China 被引量:23
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作者 FANG Yi-ping YING Bin 《Journal of Mountain Science》 SCIE CSCD 2016年第6期1120-1138,共19页
China is a mountainous country with a great diversity of landform and geomorphology.This diversity underlines the need for regionalization and classification.This study defines the mountain terrains and regions with t... China is a mountainous country with a great diversity of landform and geomorphology.This diversity underlines the need for regionalization and classification.This study defines the mountain terrains and regions with three criteria-elevation,relative height,and slope,and examines the extent of mountainous regions by using county as the basic administrative unit.According to the three parameters of economic base,resident income and development potential,we classified the economic development level in mountainous regions of China.The findings reveal that the extent of the mountainous region accounts for 74.9% of the China's Mainland's total area.The economic development of mountainous regions in China is classified into 4 main types and 23 subtypes. 展开更多
关键词 Mountain terrain Mountainous region Classification system Economic development China
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Design and Implementation of Weibo Sentiment Analysis Based on LDA and Dependency Parsing 被引量:4
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作者 Yonggan Li Xueguang Zhou +1 位作者 Yan Sun Huanguo Zhang 《China Communications》 SCIE CSCD 2016年第11期91-105,共15页
Information content security is a branch of cyberspace security. How to effectively manage and use Weibo comment information has become a research focus in the field of information content security. Three main tasks i... Information content security is a branch of cyberspace security. How to effectively manage and use Weibo comment information has become a research focus in the field of information content security. Three main tasks involved are emotion sentence identification and classification,emotion tendency classification,and emotion expression extraction. Combining with the latent Dirichlet allocation(LDA) model,a Gibbs sampling implementation for inference of our algorithm is presented,and can be used to categorize emotion tendency automatically with the computer. In accordance with the lower ratio of recall for emotion expression extraction in Weibo,use dependency parsing,divided into two categories with subject and object,summarized six kinds of dependency models from evaluating objects and emotion words,and proposed that a merge algorithm for evaluating objects can be accurately evaluated by participating in a public bakeoff and in the shared tasks among the best methods in the sub-task of emotion expression extraction,indicating the value of our method as not only innovative but practical. 展开更多
关键词 information security information content security sentiment analysis dependency parsing emotion tendency classification emotion expression extraction
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Construction and diversity analysis of a murine IgEphage surface display library
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作者 LIZONGDONG MINGYEH 《Cell Research》 SCIE CAS CSCD 1997年第2期161-170,共10页
To make further investigation of the IgE antibodyrepertoire in Trichosanthin (TCS) allergic responses, amurine IgE phage surface display library was constructed(3.0×105 independent clones). We first constructed t... To make further investigation of the IgE antibodyrepertoire in Trichosanthin (TCS) allergic responses, amurine IgE phage surface display library was constructed(3.0×105 independent clones). We first constructed theVe cDNA library (4.6×105 independent clones) and VκcDNA library (3.0×105 independent clones). Then, theVε and Vκ gene segments were amplified from both libraries by PCR respectively, and assembled into Fab fragment by SOE PCR. The phage library containing Fabs wasthus constructed. The diversity of Vε from this library wasanalyzed and proved. Fab clones with high specificity toTCS have been screened out. 展开更多
关键词 Phage surface display library trichosanthin murine IgE antibody
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