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探讨样本吸附反应杯对临床检验结果的影响及解决办法
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作者 逯阵毫 王玉堂 +2 位作者 李林 付光宇 吴学炜 《中国医学工程》 2020年第6期9-11,共3页
目的研究磁微粒化学发光平台中,样本吸附反应杯造成检测结果的假阳性,为医学实验室提供一种研究样本吸附的方法,并提出相关解决办法。方法首先对磁微粒化学发光和酶联免疫吸附测定(ELISA)平台间测试结果进行比对,确认平台差异标本,然后... 目的研究磁微粒化学发光平台中,样本吸附反应杯造成检测结果的假阳性,为医学实验室提供一种研究样本吸附的方法,并提出相关解决办法。方法首先对磁微粒化学发光和酶联免疫吸附测定(ELISA)平台间测试结果进行比对,确认平台差异标本,然后通过替换磁微粒化学发光试剂盒中磁微粒组分,查找造成平台差异的主要原因,最后在样本稀释液中加入表面活性剂,试图通过表面活性剂的作用,使磁微粒化学发光平台与ELISA平台检测结果一致。结果5例样本出现平台间差异,其在磁微粒平台全部为阳性,ELISA平台全部为阴性;将磁微粒试剂盒中磁微粒组分替换成缓冲液后,其检测结果仍为阳性,证实磁微粒平台样本与反应杯发生非特异性结合;通过在样本稀释液中添加0.5%Triton X-100后,5例样本在磁微粒化学发光平台的假阳性现象消失。结论磁微粒化学发光平台检测结果易受非特异性结合因素影响,特别是在间接法免疫分析中,人体内免疫球蛋白非特异性结合在反应杯上,导致检测结果偏高,在样本稀释过程中加入适当浓度的表面活性剂可以降低此现象的发生概率。 展开更多
关键词 样本吸附 反应杯 假阳性 表面活性剂 非特异性结合
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Beneficiation study of Eshidiya phosphorites using a rotary triboelectrostatic separator 被引量:12
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作者 TAO Daniel AL-HWAITI Mohammad 《Mining Science and Technology》 EI CAS 2010年第3期357-364,371,共9页
This study was performed to investigate the feasibility of applying a Rotary Triboelectrostatic Separator(RTS) to the beneficiation of Eshidiya phosphate minerals.RTS separation tests were carried out on phosphatic ... This study was performed to investigate the feasibility of applying a Rotary Triboelectrostatic Separator(RTS) to the beneficiation of Eshidiya phosphate minerals.RTS separation tests were carried out on phosphatic bed A_1,phosphatic bed A_3 and slime samples.The bed A_1 and slime samples were tested without desliming.Two sets of tests were performed using the A_3 sample: one was performed without desliming and the other with the A_3 sample deslimed.RTS separation tests as initially performed on the bed A_1 and slime samples gave products that had essentially the same P_2O_5 content.This indicated that adsorbed clay particles on the phosphate surface are responsible for the poor separation of un-deslimed phosphates.Better triboelectrostatic separation was observed with the undeslimed A_3 phosphate sample;these tests resulted in a highest product grade of 26%P_2O_5.The deslimed A_3 sample showed far more effective separation than the undeslimed A_3 one.In fact,a concentrate of 34%P_2O_5 was obtained from the triboelectrcstatic separation of deslimed A_3.The results indicate that with deslimed A_3 P_2O_5 recovery was about 65%for a concentrate of 28%P_2O_5 and about 45%for a concentrate of 30%P_2O_5.These results clearly show the importance of desliming for effective beneficiation of phosphate by the RTS.A more efficient separation can be expected from optimized operating conditions and circuit configuration. 展开更多
关键词 BENEFICIATION triboelectrostatic separation PHOSPHATE Eshidiya JORDAN
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