AIM:To comparatively evaluate PCR and other diagnostic methods (the rapid urease test and/or culture) in order to determine which of the three PCR methods (ureA,glmM and 26-kDa,SSA gene) was most appropriate in the di...AIM:To comparatively evaluate PCR and other diagnostic methods (the rapid urease test and/or culture) in order to determine which of the three PCR methods (ureA,glmM and 26-kDa,SSA gene) was most appropriate in the diagnosis of Helicobacterpylori(Hpylori) infection and also to evaluate the detection of a putative virulence marker of H pylori,the cage,gene,by PCR in biopsy specimens. METHODS:One hundred and eighty-nine biopsy specimens were collected from 63 patients (three biopsies each) undergoing upper gastroduodenal endoscopy for various dyspeptic symptoms.The PCR methods used to detect H pylori DNA directly from biopsies were the glmM,26-kDa, ureA and then cagA was used to compare the culture technique and CLO for urease with the culture technique being used as the gold standard. RESULTS:Thirty-five percent of the biopsies were positive for H pylori DNA using the 3 PCR methods,while 68% of these were positive for the cagA gene.Twenty-four percent of the biopsies were negative for H pylori DNA in all PCR methods screened.The remaining 41% were either positive for ureA gene only,glmM only,26-kDa only,or ureA+glmM, ureA+26-kDa,glmM+26-kDa.Out of the 35% positive biopsies,41% and 82% were positive by culture and CLO respectively,while all negative biopsies were also negative by culture and cagA.Cag A+ infection was also predominantly found in H pylori DNA of the biopsies irrespective of the clinical diagnosis. CONCLUSION:This method is useful for correctly identifying infections caused by H pylori and can be easily applied in our laboratory for diagnostic purposes.展开更多
This study was conducted to find out the available visual diversity in Japanese residential streetscapes. The visual diversity occurs when the visual elements on streetscapes are different to each other. In this study...This study was conducted to find out the available visual diversity in Japanese residential streetscapes. The visual diversity occurs when the visual elements on streetscapes are different to each other. In this study, a field survey as a subjective method and the entropy as an objective method were used as measures of visual diversity. Data were collected from 80 residential streetscapes from four sites in the Saitama Prefecture, Japan. The sequential perspective views of streetscapes were analyzed for eight visual elements： shape of houses, shape of roofs, shape of windows, positions of the main openings of houses, availability of the ornaments on houses, height, color and the appearance of the boundary walls of premises to calculate the entropy. A field survey with multicultural subjects was used to find out the correlation between entropy and the human perception on the visual diversity. According to the results, the residential streetscapes displayed high diversity variations largely due to the shape of the roofs, shape of the windows and the appearance of the boundary walls of the premises. Moreover, the results concluded that the residential streetscapes at the study area are neither visually diverse nor visually uniform in considering the visual diversity aspect.展开更多
We consider an iterative phase synchronization scheme based on maximum a posteriori probability algorithm.In classical approaches,the phase noise estimation model considers one sample per symbol at the channel and rec...We consider an iterative phase synchronization scheme based on maximum a posteriori probability algorithm.In classical approaches,the phase noise estimation model considers one sample per symbol at the channel and receiver.However,information theoretic studies suggested use of more than one sample per symbol at the channel and receiver for achieving higher performance.In this article,a soft-information aided iterative receiver is derived,which uses off-the-shelf blocks for detection and demodulation by keeping the complexity of the receiver acceptable.We consider here two samples per symbols at the channel and receiver in a pragmatic paradigm.It is shown that phase noise estimation can be significantly improved at the expense of modest processing overhead.Simulation results are presented for low-density parity check coded quadrature amplitude modulations.Our results show a significant performance improvement for strong phase noise values compared to classical receiver approaches.展开更多
The study was conducted for the detection of Listeria monocytogenes bacteria from different sources （CSF and blood） obtained from patients in Pediatric hospital and from food sources like （yogurt, raw vegetables an...The study was conducted for the detection of Listeria monocytogenes bacteria from different sources （CSF and blood） obtained from patients in Pediatric hospital and from food sources like （yogurt, raw vegetables and raw milk, sausage）. Ten isolates were isolated from 150 specimens one of them from CSF and one isolate isolated from blood samples the others isolated from food specimens 6 isolates isolated from sausage and 2 from raw vegetables. Isolates were identified traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties, identification by Api listeria was done. Determine the isolates that produce biofilm by tissue culture plate method. The highest biofilm forming strains ofListeria monocytogenes isolates appear in No. （D10, El, E5 and E7） OD reading each of them is （0.13, 0.09, 0.11 and 0.19） respectively, the lowest or poor biofilm forming strains appear in No. （D11, D12, E2, E3, E4 and E6） that optical density （OD） reading are （0.04, 0.03, 0.05, 0.04, 0.05 and 0.03） respectively by comparing with control prepared in well （A12） that stained by crystal violate without putting any isolates and the OD reading is （0.003）. Confirmation by PCR was done only for four isolates that produce biofilm （D10 and El） that obtained from CSF and blood sample and for （E5 and E7） that obtained from food samples.展开更多
Fixed-time control of traffic signals pursues the regulation of phases based on historical data of traffic demand, in this way, neglecting of the random arrival rates of traffic flow on different intersection streams ...Fixed-time control of traffic signals pursues the regulation of phases based on historical data of traffic demand, in this way, neglecting of the random arrival rates of traffic flow on different intersection streams causes increasing of the stops and delays and fuel consumption at the same time. Coordinated semi-actuated control due to ability to respond traffic demands on both main and secondary directions, based on road detector registration saves the coordinated features, serving the unused time to the main road, while the secondary clears early. In this paper, the authors analyzed and explained comparatively the results of LOS （level of service） parameters of the current state of control （fixed-time） with the proposed control （semi-actuated coordinated） of the artery of length 2,348 km consisted of four signalized T intersections. Highway Capacity Manual and Synchro/Sim Traffic software are used for analysis and optimization of parameters in this paper.展开更多
基金Supported by Inserm Fellowship,France,awarded to Dr.SI Smith
文摘AIM:To comparatively evaluate PCR and other diagnostic methods (the rapid urease test and/or culture) in order to determine which of the three PCR methods (ureA,glmM and 26-kDa,SSA gene) was most appropriate in the diagnosis of Helicobacterpylori(Hpylori) infection and also to evaluate the detection of a putative virulence marker of H pylori,the cage,gene,by PCR in biopsy specimens. METHODS:One hundred and eighty-nine biopsy specimens were collected from 63 patients (three biopsies each) undergoing upper gastroduodenal endoscopy for various dyspeptic symptoms.The PCR methods used to detect H pylori DNA directly from biopsies were the glmM,26-kDa, ureA and then cagA was used to compare the culture technique and CLO for urease with the culture technique being used as the gold standard. RESULTS:Thirty-five percent of the biopsies were positive for H pylori DNA using the 3 PCR methods,while 68% of these were positive for the cagA gene.Twenty-four percent of the biopsies were negative for H pylori DNA in all PCR methods screened.The remaining 41% were either positive for ureA gene only,glmM only,26-kDa only,or ureA+glmM, ureA+26-kDa,glmM+26-kDa.Out of the 35% positive biopsies,41% and 82% were positive by culture and CLO respectively,while all negative biopsies were also negative by culture and cagA.Cag A+ infection was also predominantly found in H pylori DNA of the biopsies irrespective of the clinical diagnosis. CONCLUSION:This method is useful for correctly identifying infections caused by H pylori and can be easily applied in our laboratory for diagnostic purposes.
文摘This study was conducted to find out the available visual diversity in Japanese residential streetscapes. The visual diversity occurs when the visual elements on streetscapes are different to each other. In this study, a field survey as a subjective method and the entropy as an objective method were used as measures of visual diversity. Data were collected from 80 residential streetscapes from four sites in the Saitama Prefecture, Japan. The sequential perspective views of streetscapes were analyzed for eight visual elements： shape of houses, shape of roofs, shape of windows, positions of the main openings of houses, availability of the ornaments on houses, height, color and the appearance of the boundary walls of premises to calculate the entropy. A field survey with multicultural subjects was used to find out the correlation between entropy and the human perception on the visual diversity. According to the results, the residential streetscapes displayed high diversity variations largely due to the shape of the roofs, shape of the windows and the appearance of the boundary walls of the premises. Moreover, the results concluded that the residential streetscapes at the study area are neither visually diverse nor visually uniform in considering the visual diversity aspect.
文摘We consider an iterative phase synchronization scheme based on maximum a posteriori probability algorithm.In classical approaches,the phase noise estimation model considers one sample per symbol at the channel and receiver.However,information theoretic studies suggested use of more than one sample per symbol at the channel and receiver for achieving higher performance.In this article,a soft-information aided iterative receiver is derived,which uses off-the-shelf blocks for detection and demodulation by keeping the complexity of the receiver acceptable.We consider here two samples per symbols at the channel and receiver in a pragmatic paradigm.It is shown that phase noise estimation can be significantly improved at the expense of modest processing overhead.Simulation results are presented for low-density parity check coded quadrature amplitude modulations.Our results show a significant performance improvement for strong phase noise values compared to classical receiver approaches.
文摘The study was conducted for the detection of Listeria monocytogenes bacteria from different sources （CSF and blood） obtained from patients in Pediatric hospital and from food sources like （yogurt, raw vegetables and raw milk, sausage）. Ten isolates were isolated from 150 specimens one of them from CSF and one isolate isolated from blood samples the others isolated from food specimens 6 isolates isolated from sausage and 2 from raw vegetables. Isolates were identified traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties, identification by Api listeria was done. Determine the isolates that produce biofilm by tissue culture plate method. The highest biofilm forming strains ofListeria monocytogenes isolates appear in No. （D10, El, E5 and E7） OD reading each of them is （0.13, 0.09, 0.11 and 0.19） respectively, the lowest or poor biofilm forming strains appear in No. （D11, D12, E2, E3, E4 and E6） that optical density （OD） reading are （0.04, 0.03, 0.05, 0.04, 0.05 and 0.03） respectively by comparing with control prepared in well （A12） that stained by crystal violate without putting any isolates and the OD reading is （0.003）. Confirmation by PCR was done only for four isolates that produce biofilm （D10 and El） that obtained from CSF and blood sample and for （E5 and E7） that obtained from food samples.
文摘Fixed-time control of traffic signals pursues the regulation of phases based on historical data of traffic demand, in this way, neglecting of the random arrival rates of traffic flow on different intersection streams causes increasing of the stops and delays and fuel consumption at the same time. Coordinated semi-actuated control due to ability to respond traffic demands on both main and secondary directions, based on road detector registration saves the coordinated features, serving the unused time to the main road, while the secondary clears early. In this paper, the authors analyzed and explained comparatively the results of LOS （level of service） parameters of the current state of control （fixed-time） with the proposed control （semi-actuated coordinated） of the artery of length 2,348 km consisted of four signalized T intersections. Highway Capacity Manual and Synchro/Sim Traffic software are used for analysis and optimization of parameters in this paper.
