We isolated and purified mitochondria from mouse livers and spinach leaves. When added into egg extracts of Xenopus laevis, they caused nuclei of mouse liver to undergo apoptotic changes. Chromatin condensation, margi...We isolated and purified mitochondria from mouse livers and spinach leaves. When added into egg extracts of Xenopus laevis, they caused nuclei of mouse liver to undergo apoptotic changes. Chromatin condensation, margination and DNA ladder were observed. After incubating isolated mitochondria in some hypotonic solutions, and centrifuging these mixtures at high speed, we got mitochondrial supernatants. It was found that in the absence of cytosolic factor, the supernatant alone was able to induce apoptotic changes in nuclei. The effective components were partly of protein. DNA fragmentation was partly inhibited by caspase inhibitors AC-DEVD-CHO and AC- YVADCHO. Meanwhile, caspase inhibitors fully blocked chromatin condensation. Primary characterization of the nuclear endonuclease(s) induced by mitochondrial supernatants was also conducted. It was found that this endonuclease is different from endonuclease G, cytochrome c-induced nuclease, or Ca2+-activated endonuclease.展开更多
目的:观察芪参固肾方结合健脾益肾针刺治疗慢性肾小球肾炎(CGN)的临床疗效。方法:按照随机数字表法将116例CGN患者分为治疗组(57例)和对照组(59例)。对照组患者采用缬沙坦胶囊口服,80 mg/次,1次/d,连续服用8周。治疗组患者在对照组治疗...目的:观察芪参固肾方结合健脾益肾针刺治疗慢性肾小球肾炎(CGN)的临床疗效。方法:按照随机数字表法将116例CGN患者分为治疗组(57例)和对照组(59例)。对照组患者采用缬沙坦胶囊口服,80 mg/次,1次/d,连续服用8周。治疗组患者在对照组治疗基础上口服芪参固肾方及健脾益肾针刺激治疗,健脾益肾针刺取穴足三里、三阴交、关元、脾俞、肾俞,每天针刺1次,每周6次,共治疗8周。比较两组患者治疗前后中医证候主症评分,并比较两组患者血肌酐(Cr)、尿素氮(BUN)、24 h尿蛋白定量(24 h Upro)、外周血单个核细胞(PBMC)凋亡率、血清单核细胞趋化蛋白-1(MCP-1)及转化生长因子-β1(TGF-β1),并评价临床疗效。结果:治疗组和对照组分别有3例、5例患者失访,两组各有54例完成研究。治疗组患者临床疗效优于对照组,差异有统计学意义(P<0.05)。治疗后两组患者腰膝酸软、倦怠乏力、胃脘胀满、浮肿评分均降低(P<0.01),且治疗组患者治疗后中医证候评分均低于对照组,差异均有统计学意义(P<0.01)。与治疗前比较,治疗后两组患者Cr、BUN、24h Upro、PBMC凋亡率、MCP-1、TGF-β1水平均降低(P<0.01),且治疗组患者治疗后Cr、BUN、24 h Upro、PBMC凋亡率、MCP-1、TGF-β1水平均低于对照组,差异均有统计学意义(P<0.01)。结论:芪参固肾方结合健脾益肾针刺治疗CGN效果显著,可有效缓解患者症状,改善肾功能,其机制可能与降低PBMC凋亡率,下调血清MCP-1、TGF-β1水平有关。展开更多
基金the National Natural Science Fundation of China(Grant No. 39800075 and 19890380) and the special Funds for Major StateBasic
文摘We isolated and purified mitochondria from mouse livers and spinach leaves. When added into egg extracts of Xenopus laevis, they caused nuclei of mouse liver to undergo apoptotic changes. Chromatin condensation, margination and DNA ladder were observed. After incubating isolated mitochondria in some hypotonic solutions, and centrifuging these mixtures at high speed, we got mitochondrial supernatants. It was found that in the absence of cytosolic factor, the supernatant alone was able to induce apoptotic changes in nuclei. The effective components were partly of protein. DNA fragmentation was partly inhibited by caspase inhibitors AC-DEVD-CHO and AC- YVADCHO. Meanwhile, caspase inhibitors fully blocked chromatin condensation. Primary characterization of the nuclear endonuclease(s) induced by mitochondrial supernatants was also conducted. It was found that this endonuclease is different from endonuclease G, cytochrome c-induced nuclease, or Ca2+-activated endonuclease.
文摘目的:观察芪参固肾方结合健脾益肾针刺治疗慢性肾小球肾炎(CGN)的临床疗效。方法:按照随机数字表法将116例CGN患者分为治疗组(57例)和对照组(59例)。对照组患者采用缬沙坦胶囊口服,80 mg/次,1次/d,连续服用8周。治疗组患者在对照组治疗基础上口服芪参固肾方及健脾益肾针刺激治疗,健脾益肾针刺取穴足三里、三阴交、关元、脾俞、肾俞,每天针刺1次,每周6次,共治疗8周。比较两组患者治疗前后中医证候主症评分,并比较两组患者血肌酐(Cr)、尿素氮(BUN)、24 h尿蛋白定量(24 h Upro)、外周血单个核细胞(PBMC)凋亡率、血清单核细胞趋化蛋白-1(MCP-1)及转化生长因子-β1(TGF-β1),并评价临床疗效。结果:治疗组和对照组分别有3例、5例患者失访,两组各有54例完成研究。治疗组患者临床疗效优于对照组,差异有统计学意义(P<0.05)。治疗后两组患者腰膝酸软、倦怠乏力、胃脘胀满、浮肿评分均降低(P<0.01),且治疗组患者治疗后中医证候评分均低于对照组,差异均有统计学意义(P<0.01)。与治疗前比较,治疗后两组患者Cr、BUN、24h Upro、PBMC凋亡率、MCP-1、TGF-β1水平均降低(P<0.01),且治疗组患者治疗后Cr、BUN、24 h Upro、PBMC凋亡率、MCP-1、TGF-β1水平均低于对照组,差异均有统计学意义(P<0.01)。结论:芪参固肾方结合健脾益肾针刺治疗CGN效果显著,可有效缓解患者症状,改善肾功能,其机制可能与降低PBMC凋亡率,下调血清MCP-1、TGF-β1水平有关。
