目的观察脐血源单核细胞分化为树突状细胞(DC)的过程中,核转录因子-k B p65(NF-k B p65)的表达以及GPS对单核细胞分化为DC的影响。方法无菌条件下采集脐血,非连续密度梯度离心获取脐血单核细胞;用含粒单细胞刺激因子(GM-CSF)、白介素-4(...目的观察脐血源单核细胞分化为树突状细胞(DC)的过程中,核转录因子-k B p65(NF-k B p65)的表达以及GPS对单核细胞分化为DC的影响。方法无菌条件下采集脐血,非连续密度梯度离心获取脐血单核细胞;用含粒单细胞刺激因子(GM-CSF)、白介素-4(IL-4)、脂多糖(LPS)和GPS的RPMI-1640培养液分别对细胞因子组、GPS组、联合培养组的单核细胞进行体外培养;应用倒置相差显微镜、瑞氏染色和CD86、CD83、HLA-DR、S-100的免疫细胞化学法鉴定单核细胞和DC。动态观察单核细胞分化为DC过程中不同时间点的NF-k B p65的表达,并进行图像和SPSS软件分析。结果 CD86、CD83、HLADR、S-100的免疫细胞化学法证实所获单核细胞和DC符合各自的形态和表型特征。在含GM-CSF和IL-4的RPMI-1640培养液培养7d时,单核细胞分化成未成熟DC(im DC);在含GM-CSF、IL-4和LPS的RPMI-1640培养液继续培养5d时,imDC分化为成熟DC(m DC),两者的细胞核内NF-k B p65表达具有显著性差异(P<0.05)。单纯用只含GPS的RPMI-1640培养液培养单核细胞至7d时,可见其细胞核呈NF-k Bp65阳性,已分化为im DC,继续培养5d,则分化为DC。用含GMCSF、IL-4和甘草多糖的RPMI-1640培养液联合培养7 d时,则该单核细胞分化为im DC;加入LPS后继续培养5 d,im DC的细胞核呈NF-k Bp65强阳性,免疫细胞化学法证实im DC已经分化为DC。联合培养组细胞的表型表达均强于细胞因子组和GPS组(P<0.05)。结论脐血来源单核细胞分化为im DC和m DC过程中,细胞核内NF-k B p65表达逐渐增强。单纯用GPS(400μg/m L)可以刺激单核细胞分化为im DC和m DC,效果逊于细胞因子GM-CSF、IL-4和LPS共同培养。展开更多
We modify the square of virtual photon four-momentum by using nuclear binding energy formula, and calculate the effect of nuclear binding energy to K factor and Compton subprocess and annihilate subprocess in A-A coll...We modify the square of virtual photon four-momentum by using nuclear binding energy formula, and calculate the effect of nuclear binding energy to K factor and Compton subprocess and annihilate subprocess in A-A collision Drell-Yan process. The outcome indicates that the effect of nuclear binding energy to K factor is obvious in little x region and it would disappear gradually as x increases.展开更多
基金The project supported by the Natural Science Foundation of Hebei Province of China under Grant No. A2005000535
文摘We modify the square of virtual photon four-momentum by using nuclear binding energy formula, and calculate the effect of nuclear binding energy to K factor and Compton subprocess and annihilate subprocess in A-A collision Drell-Yan process. The outcome indicates that the effect of nuclear binding energy to K factor is obvious in little x region and it would disappear gradually as x increases.
文摘目的:探讨生长激素(GH)对实验大鼠牙齿移动过程中RANKL和BMPs表达的影响。方法:将40只7周龄雄性Wistar大鼠随机分为对照组(C)和实验组(E)。50 g力值用于近中移动左侧上颌第一磨牙。E组和C组分别腹部皮下注射GH(0.15 IU/kg/d)和等量的生理盐水。大鼠分别在第3、7、10、14 d处死。上颌第一磨牙及其牙周组织切片行RANKL、BMPs免疫组织化学染色,并进行图像分析和统计。结果:实验组在第3 d和第7 d RANKL表达明显增强(P<0.05),在第10 d和第14 d BMPs表达与对照组相比有显著性差异(P<0.05)。结论:短期注射生长激素可改变牙周组织内RANKL和BMPs的表达,表明GH参与了正畸牙齿移动过程中的骨改建。
