本文收集1986年11月以前经手术切除的胃癌40例,按五年生存期分为 A、B 两组(≥5年为 A 组,<5年为 B 组)。从粘液组织化学[AB(PH2.5)/PAS、HID/AB(PH2.5)]、免疫组织化学(CEA、ER 的表达)及核形态测量参数等方面探讨其与组织学类型及...本文收集1986年11月以前经手术切除的胃癌40例,按五年生存期分为 A、B 两组(≥5年为 A 组,<5年为 B 组)。从粘液组织化学[AB(PH2.5)/PAS、HID/AB(PH2.5)]、免疫组织化学(CEA、ER 的表达)及核形态测量参数等方面探讨其与组织学类型及预后的关系。结果发现即使是低分化型癌,分泌酸性粘液者预后较好;ER 阳性者较阴性者好;而 CEA 表达与预后无关;核形态测量在高分化型癌与预后有关,低分化型癌 B 组的细胞核具有异型性,但核面积与核周长的值均小于 A 组,有参考价值。展开更多
AIM: To investigate the association of nuclear morphometry and DNA content with resectability of pancreatic cancer.METHODS: A total of 36 patients with pancreatic adenocarcinoma were divided into resectable group and ...AIM: To investigate the association of nuclear morphometry and DNA content with resectability of pancreatic cancer.METHODS: A total of 36 patients with pancreatic adenocarcinoma were divided into resectable group and unresectable group. The nuclear morphometry and DNA contents of tumor cells were analyzed by IBAS autoimagine analyzer from paraffin-embedded materials. Localization size,histological type and grade, and clinical stage of the tumor were evaluated. Factors influencing resectability of pancreatic cancer were investigated using stepwise regression analysis.RESULTS: Statistical significance was found in nuclear DNA content (integrated optical density, IOD) of tumor cells (1.64±0.41 vs 2.96±0.55), DNA ploidy, ages (46.5±5.3 years vs 58.6±0.7 years) and tumor volumes (298.1±101.5 cm3 vs 634.7±512.5 cm3) in both groups (P＜0.05), and no difference was found in the nuclear morphometry (P＞0.05). The rates of diploid/tetraploid and aneuploid were 66.7 % and 33.3% in resectable group respectively, and 38.9 % and 62.1%in unresectable group, respectively (P＜0.05). IOD (X12), ploidy status (X13) and clinical stage (X3) were radical resectable indicators with statistical significance. The regression equation for resectability was Y=-9.2053+3.5428X12+2.5390X13-2.3001X3(RR=0.8780, P＜0.01).CONCLUSION: There is a high correlation between resectability of pancreatic cancers and their DNA contents,DNA ploidy status and clinical stage.展开更多
Two varieties of fiddleneck （Phacelia tanacetifolia Benth.） plant were determined for visualizing somatic chromosomes. The 4-5 days old root tips were pre-treated in 6% a-monobromonaphtalane in ＋4 ℃ for 7.5 h, the...Two varieties of fiddleneck （Phacelia tanacetifolia Benth.） plant were determined for visualizing somatic chromosomes. The 4-5 days old root tips were pre-treated in 6% a-monobromonaphtalane in ＋4 ℃ for 7.5 h, then fixed in glacial acetic acid for 30 min and transferred to 70% ethanol for long storage. When the root tips were analyzed, they were hydrolyzed with 1 N HCI for 13 min at room temperature （25 ℃）. After hydrolyzing, root tips were stained with 2% aceto orcein in darkness for 2.5 h. The squash method for preparation was used for chromosomal investigations. The chromosome length （C）, relative length （RL）, the long arm （L） and short arm （S） lengths, arm ratio （AR; L/S） and centromefic index （S/C） were calculated for caryologic parameters. The ideograms and detailed chromosome morphology measurements of the species were performed by the use of MicroMeasure 3.3. According to results, fiddleneck （Phacelia tanacetifolia Benth.） has 2n = 22 chromosomes and the karyotype formulas of two varieties of fiddleneck were 16 median and 6 submedian （16 m ＋ 6 sm）.展开更多
文摘本文收集1986年11月以前经手术切除的胃癌40例,按五年生存期分为 A、B 两组(≥5年为 A 组,<5年为 B 组)。从粘液组织化学[AB(PH2.5)/PAS、HID/AB(PH2.5)]、免疫组织化学(CEA、ER 的表达)及核形态测量参数等方面探讨其与组织学类型及预后的关系。结果发现即使是低分化型癌,分泌酸性粘液者预后较好;ER 阳性者较阴性者好;而 CEA 表达与预后无关;核形态测量在高分化型癌与预后有关,低分化型癌 B 组的细胞核具有异型性,但核面积与核周长的值均小于 A 组,有参考价值。
文摘AIM: To investigate the association of nuclear morphometry and DNA content with resectability of pancreatic cancer.METHODS: A total of 36 patients with pancreatic adenocarcinoma were divided into resectable group and unresectable group. The nuclear morphometry and DNA contents of tumor cells were analyzed by IBAS autoimagine analyzer from paraffin-embedded materials. Localization size,histological type and grade, and clinical stage of the tumor were evaluated. Factors influencing resectability of pancreatic cancer were investigated using stepwise regression analysis.RESULTS: Statistical significance was found in nuclear DNA content (integrated optical density, IOD) of tumor cells (1.64±0.41 vs 2.96±0.55), DNA ploidy, ages (46.5±5.3 years vs 58.6±0.7 years) and tumor volumes (298.1±101.5 cm3 vs 634.7±512.5 cm3) in both groups (P＜0.05), and no difference was found in the nuclear morphometry (P＞0.05). The rates of diploid/tetraploid and aneuploid were 66.7 % and 33.3% in resectable group respectively, and 38.9 % and 62.1%in unresectable group, respectively (P＜0.05). IOD (X12), ploidy status (X13) and clinical stage (X3) were radical resectable indicators with statistical significance. The regression equation for resectability was Y=-9.2053+3.5428X12+2.5390X13-2.3001X3(RR=0.8780, P＜0.01).CONCLUSION: There is a high correlation between resectability of pancreatic cancers and their DNA contents,DNA ploidy status and clinical stage.
文摘Two varieties of fiddleneck （Phacelia tanacetifolia Benth.） plant were determined for visualizing somatic chromosomes. The 4-5 days old root tips were pre-treated in 6% a-monobromonaphtalane in ＋4 ℃ for 7.5 h, then fixed in glacial acetic acid for 30 min and transferred to 70% ethanol for long storage. When the root tips were analyzed, they were hydrolyzed with 1 N HCI for 13 min at room temperature （25 ℃）. After hydrolyzing, root tips were stained with 2% aceto orcein in darkness for 2.5 h. The squash method for preparation was used for chromosomal investigations. The chromosome length （C）, relative length （RL）, the long arm （L） and short arm （S） lengths, arm ratio （AR; L/S） and centromefic index （S/C） were calculated for caryologic parameters. The ideograms and detailed chromosome morphology measurements of the species were performed by the use of MicroMeasure 3.3. According to results, fiddleneck （Phacelia tanacetifolia Benth.） has 2n = 22 chromosomes and the karyotype formulas of two varieties of fiddleneck were 16 median and 6 submedian （16 m ＋ 6 sm）.
