AIM: To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection. METHODS: Reverse transcribed cDNA was subjected to microarray assay. The ...AIM: To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection. METHODS: Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods. RESULTS: The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepGZ and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene named HCTP4 was cloned with molecular biological method in combination with bioinformatics method. CONCLUSION: HCV core is a potential transactivator. Microarray is an efficient and convenient method for analysis of differentially expressed genes.展开更多
The sensitivity of precipitation was studied by conducting control aqua-planet experiments(APEs) with a model to determine atmospheric general circulation.The model includes two versions: that with a spectral dynamica...The sensitivity of precipitation was studied by conducting control aqua-planet experiments(APEs) with a model to determine atmospheric general circulation.The model includes two versions: that with a spectral dynamical core(SAMIL) and that with a finite-volume dynamical core(FAMIL).Three factors were investigated including dynamical core,time-step length,and horizontal resolution.Numerical results show that the dynamical core significantly affects the structure of zonal averaged precipitation.FAMIL exhibited an equatorial precipitation belt with a single narrow peak,and SAMIL showed a broader belt with double peaks.Moreover,the time step of the model physics is shown to affect the zonal-averaged tropical convective precipitation ratio such that a longer time step leads to more production and consumption of convective available potential energy and convection initiated away from the equator,which corresponds to equatorial double peaks of precipitation.Further,precipitation is determined to be sensitive to horizontal resolution such that higher horizontal resolution allows for more small-scale kinetic energy to be resolved and leads to a broader probability distribution of low-level vertical velocity.This process results in heavier rainfall and convective precipitation extremes in the tropics.Abstract The sensitivity of precipitation was studied by conducting control aqua-planet experiments(APEs)with a model to determine atmospheric general circulation.The model includes two versions:that with a spectral dynamical core(SAMIL)and that with a finite-volume dynamical core(FAMIL).Three factors were investigated including dynamical core,time-step length,and horizontal resolution.Numerical results show that the dynamical core significantly affects the structure of zonal averaged precipitation.FAMIL exhibited an equatorial precipitation belt with a single narrow peak,and SAMIL showed a broader belt with double peaks.Moreover,the time step of the model physics is shown to affect the zonal-averaged tropical convective precipitation ratio such that a longer time step leads to more production and consumption of convective available potential energy and convection initiated away from the equator,which corresponds to equatorial double peaks of precipitation.Further,precipitation is determined to be sensitive to horizontal resolution such that higher horizontal resolution allows for more small-scale kinetic energy to be resolved and leads to a broader probability distribution of low-level vertical velocity.This process results in heavier rainfall and convective precipitation extremes in the tropics.展开更多
At the CERN large hadron collider （LHC）, production of the Higgs boson in association with Z or W bosons provides a dramatic experimental signal for detecting the standard model （SM） Higgs boson. In this paper, we...At the CERN large hadron collider （LHC）, production of the Higgs boson in association with Z or W bosons provides a dramatic experimental signal for detecting the standard model （SM） Higgs boson. In this paper, we consider the contributions of the left-right twin Higgs （LRTH） model to the processes qq＇ --~ Z（W）H. Our numerical results show that, in the favorable parameter spaces, the cross sections deviate distinctly from the predictions of the SM. The possible signals of the LRTH model can be detected via these processes at the LHC experiments.展开更多
基金Supported by the National Natural Science Foundation of China, No. 39970674
文摘AIM: To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection. METHODS: Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods. RESULTS: The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepGZ and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene named HCTP4 was cloned with molecular biological method in combination with bioinformatics method. CONCLUSION: HCV core is a potential transactivator. Microarray is an efficient and convenient method for analysis of differentially expressed genes.
基金supported by the Chinese Academy of Sciences (XDA11010402 and XDA05110303)the National Basic Research Program of China (973 Program,2012CB417203 and 2013CB955803)and the National Natural Science Foundation of China (91337110 and 41023002)
文摘The sensitivity of precipitation was studied by conducting control aqua-planet experiments(APEs) with a model to determine atmospheric general circulation.The model includes two versions: that with a spectral dynamical core(SAMIL) and that with a finite-volume dynamical core(FAMIL).Three factors were investigated including dynamical core,time-step length,and horizontal resolution.Numerical results show that the dynamical core significantly affects the structure of zonal averaged precipitation.FAMIL exhibited an equatorial precipitation belt with a single narrow peak,and SAMIL showed a broader belt with double peaks.Moreover,the time step of the model physics is shown to affect the zonal-averaged tropical convective precipitation ratio such that a longer time step leads to more production and consumption of convective available potential energy and convection initiated away from the equator,which corresponds to equatorial double peaks of precipitation.Further,precipitation is determined to be sensitive to horizontal resolution such that higher horizontal resolution allows for more small-scale kinetic energy to be resolved and leads to a broader probability distribution of low-level vertical velocity.This process results in heavier rainfall and convective precipitation extremes in the tropics.Abstract The sensitivity of precipitation was studied by conducting control aqua-planet experiments(APEs)with a model to determine atmospheric general circulation.The model includes two versions:that with a spectral dynamical core(SAMIL)and that with a finite-volume dynamical core(FAMIL).Three factors were investigated including dynamical core,time-step length,and horizontal resolution.Numerical results show that the dynamical core significantly affects the structure of zonal averaged precipitation.FAMIL exhibited an equatorial precipitation belt with a single narrow peak,and SAMIL showed a broader belt with double peaks.Moreover,the time step of the model physics is shown to affect the zonal-averaged tropical convective precipitation ratio such that a longer time step leads to more production and consumption of convective available potential energy and convection initiated away from the equator,which corresponds to equatorial double peaks of precipitation.Further,precipitation is determined to be sensitive to horizontal resolution such that higher horizontal resolution allows for more small-scale kinetic energy to be resolved and leads to a broader probability distribution of low-level vertical velocity.This process results in heavier rainfall and convective precipitation extremes in the tropics.
基金Supported by the National Natural Science Foundation of China under Grant Nos. 10975047 and 10979008
文摘At the CERN large hadron collider （LHC）, production of the Higgs boson in association with Z or W bosons provides a dramatic experimental signal for detecting the standard model （SM） Higgs boson. In this paper, we consider the contributions of the left-right twin Higgs （LRTH） model to the processes qq＇ --~ Z（W）H. Our numerical results show that, in the favorable parameter spaces, the cross sections deviate distinctly from the predictions of the SM. The possible signals of the LRTH model can be detected via these processes at the LHC experiments.
