目的探讨富集有核细胞层沉淀法制备的非小细胞肺癌(non small cell lung cancer,NSCLS)胸腔积液细胞沉渣块中的表皮生长因子受体(EGFR)基因的突变率。方法选取NSCLS伴恶性胸腔积液患者116例,应用富集有核细胞层沉淀法制备胸腔积液细胞...目的探讨富集有核细胞层沉淀法制备的非小细胞肺癌(non small cell lung cancer,NSCLS)胸腔积液细胞沉渣块中的表皮生长因子受体(EGFR)基因的突变率。方法选取NSCLS伴恶性胸腔积液患者116例,应用富集有核细胞层沉淀法制备胸腔积液细胞沉渣块,并应用突变扩增阻滞系统(ARMS)法检测胸腔积液细胞沉渣块中的EGFR基因的突变情况。结果EGFR基因突变率为50.86%,其中腺癌突变率为57.83%,非腺癌突变率为33.33%。结论富集有核细胞层沉淀法制备的NSCLS胸腔积液细胞沉渣块中EGFR基因突变率高于传统的离心细胞沉渣法,有效提高了肿瘤细胞的获得率及DNA提取率,为肺癌患者的诊断、治疗及分子生物学检测提供了有效手段。展开更多
In order to overcome the defects of air-agitated seed precipitation, such as scaring, liquid short-(circuiting,) the three-dimension flow fields with different structure are numerically simulated by computational flui...In order to overcome the defects of air-agitated seed precipitation, such as scaring, liquid short-(circuiting,) the three-dimension flow fields with different structure are numerically simulated by computational fluid dynamics software. Euler/Euler approach was used to study the effects of structure on the flow field in the tank. Multi-fluid model, body-fitted coordinates and multi-block gird were adopted in the simulation. The simulating results are well consonant with the practical situations. The flow field is improved obviously when the flow velocity increases from (0.089m/s) to 0.1920.300m/s at the bottom of the optimized tank and therefore the scaring is reduced greatly in the industrial production. With a gathering sill, the problem of short-circuiting, which always appeares in the upper of the tank, can be solved very well.展开更多
文摘目的探讨富集有核细胞层沉淀法制备的非小细胞肺癌(non small cell lung cancer,NSCLS)胸腔积液细胞沉渣块中的表皮生长因子受体(EGFR)基因的突变率。方法选取NSCLS伴恶性胸腔积液患者116例,应用富集有核细胞层沉淀法制备胸腔积液细胞沉渣块,并应用突变扩增阻滞系统(ARMS)法检测胸腔积液细胞沉渣块中的EGFR基因的突变情况。结果EGFR基因突变率为50.86%,其中腺癌突变率为57.83%,非腺癌突变率为33.33%。结论富集有核细胞层沉淀法制备的NSCLS胸腔积液细胞沉渣块中EGFR基因突变率高于传统的离心细胞沉渣法,有效提高了肿瘤细胞的获得率及DNA提取率,为肺癌患者的诊断、治疗及分子生物学检测提供了有效手段。
基金Project(030620) supported by the Dissertation Innovation Fund of Central South University
文摘In order to overcome the defects of air-agitated seed precipitation, such as scaring, liquid short-(circuiting,) the three-dimension flow fields with different structure are numerically simulated by computational fluid dynamics software. Euler/Euler approach was used to study the effects of structure on the flow field in the tank. Multi-fluid model, body-fitted coordinates and multi-block gird were adopted in the simulation. The simulating results are well consonant with the practical situations. The flow field is improved obviously when the flow velocity increases from (0.089m/s) to 0.1920.300m/s at the bottom of the optimized tank and therefore the scaring is reduced greatly in the industrial production. With a gathering sill, the problem of short-circuiting, which always appeares in the upper of the tank, can be solved very well.
