目的:研究黄芩苷对大鼠肠缺血再灌注(intestinal ischemia-reperfusion,IIR)损伤的保护作用及机制.方法:♂Wistar大鼠32只,随机分为假手术(sham-operated,SO)组、黄芩苷(baicalin,BA)组、肠缺血再灌注(IIR)组、黄芩苷+肠缺血再灌注(BA+I...目的:研究黄芩苷对大鼠肠缺血再灌注(intestinal ischemia-reperfusion,IIR)损伤的保护作用及机制.方法:♂Wistar大鼠32只,随机分为假手术(sham-operated,SO)组、黄芩苷(baicalin,BA)组、肠缺血再灌注(IIR)组、黄芩苷+肠缺血再灌注(BA+IIR)组.BA组和BA+IIR组于造模前30 min腹腔注射黄芩苷100 mg/kg体质量,SO组和IIR组注射等量生理盐水.再灌注2 h后取材,HE染色观察小肠组织损伤程度,水溶性四唑盐1(water soluble tetrazolium s a l t 1,W S T-1)法测定血清超氧化物歧化酶(superoxide dismutase,SOD)活性,硫代巴比妥酸法测定血清丙二醛(malondialdehyde,MDA)含量,ELISA法检测血浆D-乳酸含量,免疫组织化学(immunohistochemistry,IHC)和Western blot法检测核因子相关因子2(NF-E2-related factor 2,Nrf2)、血红素加氧酶1(heme oxygenase 1,HO-1)蛋白表达.结果:IIR组与SO组比较,小肠黏膜损伤Chiu氏评分升高(3.99±0.24 vs 0.30±0.08,P<0.01),血清S O D活性降低(100.08 U/m L±3.20 U/mL vs 136.88 U/mL±5.93 U/mL,P<0.01),MDA含量增加(3.41 nmol/mL±0.23nmol/mL vs 1.59 nmol/mL±0.25 nmol/mL,P<0.01),血浆D-乳酸含量增多(174.27 ng/mL±33.84 ng/mL vs 52.40 ng/mL±12.12 ng/mL,P<0.01),小肠组织中N r f2、H O-1表达增加(IHC:0.326±0.024 vs 0.289±0.041,0.298±0.025 vs 0.258±0.027,P<0.05;WB:1.062±0.056 vs 0.584±0.048,1.019±0.041 vs 0.592±0.037,P<0.01).BA组小肠组织Nrf2、HO-1表达比SO组显著增加(IHC:0.322±0.028vs 0.289±0.041,0.284±0.009 vs 0.258±0.027,P<0.05;WB:1.077±0.038 vs 0.584±0.048,1.027±0.042 vs 0.592±0.037,P<0.01).BA+IIR组与IIR组相比,小肠黏膜损伤Chiu氏评分降低(2.95±0.26 vs 3.99±0.24,P<0.01),血清SOD活性升高(116.11 U/mL±4.12 U/mL vs 100.08 U/mL±3.20 U/mL,P<0.01),MDA含量减少(3.09 nmol/mL±0.15 nmol/mL vs 3.41nmol/mL±0.23 nmol/mL,P<0.01),血浆D-乳酸含量减少(108.04 ng/mL±20.19 ng/mL vs174.27 ng/mL±33.84 ng/mL,P<0.01),小肠组织Nrf2、HO-1表达增加(IHC:0.371±0.024 vs0.326±0.024,0.336±0.031 vs 0.298±0.025,P<0.01;WB:1.541±0.100 vs 1.062±0.056,1.458±0.071 vs 1.019±0.041,P<0.01).结论:黄芩苷对大鼠肠缺血再灌注损伤有良好的保护作用,其机制可能是通过激活核因子相关因子2-抗氧化反应元件(NF-E2-related factor 2-antioxidant response element,Nrf2-ARE)通路进而增加抗氧化酶SOD、HO-1表达实现的.展开更多
Follicle stimulating hormone β (FSHβ) of Japanese flounder (Paralichthys olivaceus) plays a key role in the regulation of gonadal development.This study aimed to investigate molecular genetic characteristics of the ...Follicle stimulating hormone β (FSHβ) of Japanese flounder (Paralichthys olivaceus) plays a key role in the regulation of gonadal development.This study aimed to investigate molecular genetic characteristics of the FSHβ gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSHβ on reproductive traits in Japanese flounder.We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the FSHβ gene in 60 individuals.We identified only an SNP (T/C) in the coding region of exon3 of FSHβ.The SNP (T/C) did not lead to amino acid changes at the position 340 bp of FSHβ gene.Statistical analysis showed that the SNP was significantly associated with testosterone (T) level and gonadosomatic index (GSI) (P<0.05).Individuals with genotype TC of the SNP had significantly higher serum T levels and GSI (P<0.05) than that of genotype CC.Therefore, FSHβ gene could be a useful molecular marker in selection for prominent reproductive trait in Japanese Flounder.展开更多
Within the framework of the U<SUB>sdpf</SUB>(16) interacting boson model (IBM), the effects of strong correlations of the dipole (p<SUP>?</SUP>-boson) and the octupole (f<SUP>?</SUP>...Within the framework of the U<SUB>sdpf</SUB>(16) interacting boson model (IBM), the effects of strong correlations of the dipole (p<SUP>?</SUP>-boson) and the octupole (f<SUP>?</SUP>-boson) degree of freedom on the positive-parity states of even-even nuclei in SU(3) limit are discussed. It is shown that configurations of an even number of many p- and f-bosons can not only be incorporated into the usual low-lying collective rotational bands, such as the ground state band, β- and γ-vibrational bands, but also naturally form the rotational bands, etc. These results are similar to that of U<SUB>sdg</SUB>(15)-IBM and in good agreement with the experimental data of the nucleus. Besides, several intraband E2 transition probabilities are given, which are consistent with that of U<SUB>sd</SUB>(6)-IBM.展开更多
The aim of this study is to explore potential pathogenicity of Mycoplasma penetrans, and to investigate whether M. penetrans lipid-associated membrane proteins （LAMPs） could induce human monocytic cell line （THP- 1...The aim of this study is to explore potential pathogenicity of Mycoplasma penetrans, and to investigate whether M. penetrans lipid-associated membrane proteins （LAMPs） could induce human monocytic cell line （THP- 1 ） to produce some proinflammatory cytokines in vitro, including interleukin- 1β （ IL- 1β）, tumor necrosis factor alpha （TNF-α）, and IL-8. THP-1 was stimulated with different concentrations of M.penetrans LAMPs and at different time to analyze the production of human IL-1β, TNF-α and IL-8. The protein levels of human IL-1β, TNF-α and IL-8 were measured by enzyme-linked immunoadsorbent assay （ELISA） and the mRNA levels of these proinflammatory cytokines were detected by reverse transcriptase-PCR （RT-PCR）. It was demonstrated in the present study that the production of IL-1β, TNF-α and IL-8 increased in dose- and time-dependent manner after stimulation with M. penetrans LAMPs in THP-1 cells. M. penetrans LAMPs also induced the expression of IL-1β, TNF-α and IL-8 mRNA. The production of IL-1β, TNF-α and IL-8 and the expression of mRNA were down-regulated by pyrrolidine dithiocarbamate （PDTC）. This study demonstrated that M. penetrans LAMPs can induce the production of proinflammatory cytokines in human monocytic cells in vitro, thus suggesting that it may be an important etiological factor.展开更多
文摘目的:研究黄芩苷对大鼠肠缺血再灌注(intestinal ischemia-reperfusion,IIR)损伤的保护作用及机制.方法:♂Wistar大鼠32只,随机分为假手术(sham-operated,SO)组、黄芩苷(baicalin,BA)组、肠缺血再灌注(IIR)组、黄芩苷+肠缺血再灌注(BA+IIR)组.BA组和BA+IIR组于造模前30 min腹腔注射黄芩苷100 mg/kg体质量,SO组和IIR组注射等量生理盐水.再灌注2 h后取材,HE染色观察小肠组织损伤程度,水溶性四唑盐1(water soluble tetrazolium s a l t 1,W S T-1)法测定血清超氧化物歧化酶(superoxide dismutase,SOD)活性,硫代巴比妥酸法测定血清丙二醛(malondialdehyde,MDA)含量,ELISA法检测血浆D-乳酸含量,免疫组织化学(immunohistochemistry,IHC)和Western blot法检测核因子相关因子2(NF-E2-related factor 2,Nrf2)、血红素加氧酶1(heme oxygenase 1,HO-1)蛋白表达.结果:IIR组与SO组比较,小肠黏膜损伤Chiu氏评分升高(3.99±0.24 vs 0.30±0.08,P<0.01),血清S O D活性降低(100.08 U/m L±3.20 U/mL vs 136.88 U/mL±5.93 U/mL,P<0.01),MDA含量增加(3.41 nmol/mL±0.23nmol/mL vs 1.59 nmol/mL±0.25 nmol/mL,P<0.01),血浆D-乳酸含量增多(174.27 ng/mL±33.84 ng/mL vs 52.40 ng/mL±12.12 ng/mL,P<0.01),小肠组织中N r f2、H O-1表达增加(IHC:0.326±0.024 vs 0.289±0.041,0.298±0.025 vs 0.258±0.027,P<0.05;WB:1.062±0.056 vs 0.584±0.048,1.019±0.041 vs 0.592±0.037,P<0.01).BA组小肠组织Nrf2、HO-1表达比SO组显著增加(IHC:0.322±0.028vs 0.289±0.041,0.284±0.009 vs 0.258±0.027,P<0.05;WB:1.077±0.038 vs 0.584±0.048,1.027±0.042 vs 0.592±0.037,P<0.01).BA+IIR组与IIR组相比,小肠黏膜损伤Chiu氏评分降低(2.95±0.26 vs 3.99±0.24,P<0.01),血清SOD活性升高(116.11 U/mL±4.12 U/mL vs 100.08 U/mL±3.20 U/mL,P<0.01),MDA含量减少(3.09 nmol/mL±0.15 nmol/mL vs 3.41nmol/mL±0.23 nmol/mL,P<0.01),血浆D-乳酸含量减少(108.04 ng/mL±20.19 ng/mL vs174.27 ng/mL±33.84 ng/mL,P<0.01),小肠组织Nrf2、HO-1表达增加(IHC:0.371±0.024 vs0.326±0.024,0.336±0.031 vs 0.298±0.025,P<0.01;WB:1.541±0.100 vs 1.062±0.056,1.458±0.071 vs 1.019±0.041,P<0.01).结论:黄芩苷对大鼠肠缺血再灌注损伤有良好的保护作用,其机制可能是通过激活核因子相关因子2-抗氧化反应元件(NF-E2-related factor 2-antioxidant response element,Nrf2-ARE)通路进而增加抗氧化酶SOD、HO-1表达实现的.
基金supported by the open-fund of Key Laboratory of Fisheries Genetic Resources and Aquaculture,Chinese Academy of Fisheries Sciences of China (2008B1207)New Teacher Special Fund of Doctor of Ministry of Education of China (20090132120006)
文摘Follicle stimulating hormone β (FSHβ) of Japanese flounder (Paralichthys olivaceus) plays a key role in the regulation of gonadal development.This study aimed to investigate molecular genetic characteristics of the FSHβ gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSHβ on reproductive traits in Japanese flounder.We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the FSHβ gene in 60 individuals.We identified only an SNP (T/C) in the coding region of exon3 of FSHβ.The SNP (T/C) did not lead to amino acid changes at the position 340 bp of FSHβ gene.Statistical analysis showed that the SNP was significantly associated with testosterone (T) level and gonadosomatic index (GSI) (P<0.05).Individuals with genotype TC of the SNP had significantly higher serum T levels and GSI (P<0.05) than that of genotype CC.Therefore, FSHβ gene could be a useful molecular marker in selection for prominent reproductive trait in Japanese Flounder.
文摘Within the framework of the U<SUB>sdpf</SUB>(16) interacting boson model (IBM), the effects of strong correlations of the dipole (p<SUP>?</SUP>-boson) and the octupole (f<SUP>?</SUP>-boson) degree of freedom on the positive-parity states of even-even nuclei in SU(3) limit are discussed. It is shown that configurations of an even number of many p- and f-bosons can not only be incorporated into the usual low-lying collective rotational bands, such as the ground state band, β- and γ-vibrational bands, but also naturally form the rotational bands, etc. These results are similar to that of U<SUB>sdg</SUB>(15)-IBM and in good agreement with the experimental data of the nucleus. Besides, several intraband E2 transition probabilities are given, which are consistent with that of U<SUB>sd</SUB>(6)-IBM.
基金This study is supported by Natural Science Foundation of Hunan Province （No. 06JJ5044） a Grant from Hunan Province Department of Health （No. B2005-089）.
文摘The aim of this study is to explore potential pathogenicity of Mycoplasma penetrans, and to investigate whether M. penetrans lipid-associated membrane proteins （LAMPs） could induce human monocytic cell line （THP- 1 ） to produce some proinflammatory cytokines in vitro, including interleukin- 1β （ IL- 1β）, tumor necrosis factor alpha （TNF-α）, and IL-8. THP-1 was stimulated with different concentrations of M.penetrans LAMPs and at different time to analyze the production of human IL-1β, TNF-α and IL-8. The protein levels of human IL-1β, TNF-α and IL-8 were measured by enzyme-linked immunoadsorbent assay （ELISA） and the mRNA levels of these proinflammatory cytokines were detected by reverse transcriptase-PCR （RT-PCR）. It was demonstrated in the present study that the production of IL-1β, TNF-α and IL-8 increased in dose- and time-dependent manner after stimulation with M. penetrans LAMPs in THP-1 cells. M. penetrans LAMPs also induced the expression of IL-1β, TNF-α and IL-8 mRNA. The production of IL-1β, TNF-α and IL-8 and the expression of mRNA were down-regulated by pyrrolidine dithiocarbamate （PDTC）. This study demonstrated that M. penetrans LAMPs can induce the production of proinflammatory cytokines in human monocytic cells in vitro, thus suggesting that it may be an important etiological factor.
