Peucedanum aucheri Boiss. （Apiaceae） is a herbaceous wild plant native to Iran and is used in Iranian folk medicine as a diuretic and for the treatment of kidney disorders. Phytochemical investigation of different e...Peucedanum aucheri Boiss. （Apiaceae） is a herbaceous wild plant native to Iran and is used in Iranian folk medicine as a diuretic and for the treatment of kidney disorders. Phytochemical investigation of different extracts prepared from the aerial part of P. aucheri Boiss. resulted to the isolation of two main flavonol glycosides from methanolic extract. Using comprehensive spectroscopic methods, including 1D and 2D nuclear magnetic resonance （NMR） spectroscopy and mass spectroscopy, chemical structure of isolated compounds were determined as kaempfrol-3-o-rutinoside （nicotiflorin） and isorhamnetin-3-o-rutinoside （narcissin）. Although narcissin has previously been isolated from P. ruthenicum, to the best of our knowledge, isolation of nicotiflorin from Peucedanum genus is reported for the first time.展开更多
NMR spectroscopy and X-ray crystallography are two premium methods for determining the atomic structures of macro-biomolecular complexes.Each method has unique strengths and weaknesses.While the two techniques are hig...NMR spectroscopy and X-ray crystallography are two premium methods for determining the atomic structures of macro-biomolecular complexes.Each method has unique strengths and weaknesses.While the two techniques are highly complementary,they have generally been used separately to address the structure and functions of biomolecular complexes.In this review,we emphasize that the combination of NMR spectroscopy and X-ray crystallography offers unique power for elucidating the structures of complicated protein assemblies.We demonstrate,using several recent examples from our own laboratory,that the exquisite sensitivity of NMR spectroscopy in detecting the conformational properties of individual atoms in proteins and their complexes,without any prior knowledge of conformation,is highly valuable for obtaining the high quality crystals necessary for structure determination by X-ray crystallography.Thus NMR spectroscopy,in addition to answering many unique structural biology questions that can be addressed specifically by that technique,can be exceedingly powerful in modern structural biology when combined with other techniques including X-ray crystallography and cryo-electron microscopy.展开更多
文摘Peucedanum aucheri Boiss. （Apiaceae） is a herbaceous wild plant native to Iran and is used in Iranian folk medicine as a diuretic and for the treatment of kidney disorders. Phytochemical investigation of different extracts prepared from the aerial part of P. aucheri Boiss. resulted to the isolation of two main flavonol glycosides from methanolic extract. Using comprehensive spectroscopic methods, including 1D and 2D nuclear magnetic resonance （NMR） spectroscopy and mass spectroscopy, chemical structure of isolated compounds were determined as kaempfrol-3-o-rutinoside （nicotiflorin） and isorhamnetin-3-o-rutinoside （narcissin）. Although narcissin has previously been isolated from P. ruthenicum, to the best of our knowledge, isolation of nicotiflorin from Peucedanum genus is reported for the first time.
基金supported by grants from the Research Grants Council of Hong Kong to M.Z.supported by the National Major Basic Research Program of China (Grant No. 2011CB910500)+3 种基金the National Natural Science Foundation of China (Grant No. 31070657)the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KSCX2-YW-R-154)The NMR spectrometers used in our studies were funded by donations from the Hong Kong Jockey Club Charity Foundationthe Special Equipment Grant from RGC of Hong Kong (Grant No. SEG_HKUST06)
文摘NMR spectroscopy and X-ray crystallography are two premium methods for determining the atomic structures of macro-biomolecular complexes.Each method has unique strengths and weaknesses.While the two techniques are highly complementary,they have generally been used separately to address the structure and functions of biomolecular complexes.In this review,we emphasize that the combination of NMR spectroscopy and X-ray crystallography offers unique power for elucidating the structures of complicated protein assemblies.We demonstrate,using several recent examples from our own laboratory,that the exquisite sensitivity of NMR spectroscopy in detecting the conformational properties of individual atoms in proteins and their complexes,without any prior knowledge of conformation,is highly valuable for obtaining the high quality crystals necessary for structure determination by X-ray crystallography.Thus NMR spectroscopy,in addition to answering many unique structural biology questions that can be addressed specifically by that technique,can be exceedingly powerful in modern structural biology when combined with other techniques including X-ray crystallography and cryo-electron microscopy.
