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布氏锥虫的一种核苷酸运输系统与药物抗性有关
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作者 方元 百里 《动物医学进展》 CSCD 2000年第3期62-63,共2页
病原体的药物抗性是日益严重的问题 ,其机制尚不完全了解。一般认为细胞摄入抗布氏锥虫药大多是通过一种至今尚未被识别的不平常的腺苷运输系统发生的 ,使用酿酒酵母 ( Saccharomyces cerevisiae)以功能筛选的方法克隆了布氏锥虫指名亚... 病原体的药物抗性是日益严重的问题 ,其机制尚不完全了解。一般认为细胞摄入抗布氏锥虫药大多是通过一种至今尚未被识别的不平常的腺苷运输系统发生的 ,使用酿酒酵母 ( Saccharomyces cerevisiae)以功能筛选的方法克隆了布氏锥虫指名亚种( Trypanosoma brucei brucei)的一个编码核苷酸运输系统的基因— Tb AT1。Tb AT1在酵母中表达时 ,使酵母能摄取腺苷 ,并赋予对密胺基苯胂类药物 ( Melaminophenyl arsenicals)的敏感性。抗药锥虫藏匿缺损 Tb AT1的变种。 展开更多
关键词 布氏锥虫 药物抗性 核苷运输系统
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温性活血化瘀中药及其活性成分抗血小板聚集的作用机制 被引量:1
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作者 俞佳峰 胡翠 +2 位作者 吴先昊 王小青 李雪 《中兽医医药杂志》 CAS 2023年第3期34-40,共7页
在临床上血瘀与心血管疾病存在高度相关性,活血化瘀中药具有活血通经、散瘀行脉的功效,在防治血瘀和血栓方面发挥着重要作用。血瘀的致病机制较为复杂,与凝血系统的功能和血液流变学异常等有关,其中血小板聚集是最主要的致病因素之一,... 在临床上血瘀与心血管疾病存在高度相关性,活血化瘀中药具有活血通经、散瘀行脉的功效,在防治血瘀和血栓方面发挥着重要作用。血瘀的致病机制较为复杂,与凝血系统的功能和血液流变学异常等有关,其中血小板聚集是最主要的致病因素之一,故抗血小板聚集是防治血瘀的主要途径之一。活血化瘀中药抗血小板聚集的主要作用机制包括抑制花生四烯酸代谢途径,保持血栓素A2(TXA2)和前列环素(PGI2)在机体内的动态平衡;抑制血小板内的亚细胞器释放血管性血友病因子(vWF)、血小板因子、P-选择素和纤维蛋白原等内容物;抑制血小板膜蛋白上的受体与相应化合物结合;影响核苷酸系统,提高血小板内cAMP和cGMP的水平或者抑制其水解等。通过参阅文献并分析,发现在具抗血小板聚集功效的37味活血化瘀中药中,温性中药所占比重最大。针对温性活血化瘀中药抗血小板聚集机制的研究主要集中于花生四烯酸代谢途径和抑制血小板内容物释放,其中三七、月季花、阿魏、鸡血藤、川芎等具有多靶点的特点。有部分研究者对活血化瘀中药的活性成分进行了修饰,得到了一系列活性更高的衍生物,如阿魏、川芎等。温性活血化瘀类中药具有副作用小、药性温和、作用靶点多等特点,且效果较佳。本文对其进行综述,以期为后续的临床应用和深入研究提供参考。 展开更多
关键词 温性 活血化瘀 抗血小板聚集 花生四烯代谢 血小板内容物 血小板膜蛋白受体 核苷酸系统 作用机制
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A Molecular Phylogeny of Macaca Based on Mitochondrial Control Region Sequences 被引量:8
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作者 李青青 张亚平 《Zoological Research》 CAS CSCD 北大核心 2004年第5期385-390,共6页
Nucleotide sequences of segments of the mitochondrial control regions were analyzed to infer the phylogenetic relationships among 7 macaques.High nucleotide diversity in Macaca assamensis and relatively low diversity ... Nucleotide sequences of segments of the mitochondrial control regions were analyzed to infer the phylogenetic relationships among 7 macaques.High nucleotide diversity in Macaca assamensis and relatively low diversity in M.thibetana were found.Based on the ML tree from control regions,species in our study can roughly be sorted into three species groups except for the phylogenetic position of M.fascicularis,i.e.,silenus group,including M.leonina;sinica group,including M.arctoides,M.assamensis,and M.thibetana;and fascicularis group,including M.mulatta and M.cyclopis.A discrepancy between earlier studies (Fooden & Lanyon,1989;Tosi et al,2003a;Deinard & Smith,2001;Evans et al,1999;Hayasaka et al,1996;Morales & Melnick,1998),our result supported the hypothesis that M.fascicularis diverged earlier than M.leonina.Mitochondrial paraphyly in eastern M.mulatta (with respect to M.cyclopis) and eastern M.assamensis (with respect to M.thibetana) were clearly observed in our study.In accordance with the results of Y chromosome,allozyme,nuclear genes and some morphological data (Delson,1980;Fooden & Lanyon,1989;Fooden,1990;Tosi et al,2000,2003a,b;Deinard & Smith,2001),our study on control region sequences supported M.arctoides to be classified into the sinica group.However,this result disagreed with the previous mtDNA studies (Hayasaka et al,1996;Morales & Melnick,1998;Tosi et al,2003a). 展开更多
关键词 MACACA MACAQUE Mitochondrial DNA Control region PHYLOGENY
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Population genetic structure of the mantis shrimp Oratosquilla oratoria(Crustacea:Squillidae)in the Yellow Sea and East China Sea 被引量:2
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作者 YANG Mei LI Xinzheng 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2018年第3期905-912,共8页
The mantis shrimp O ratosquilla oratoria is an ecologically and economically important species in the Western Pacific. In present study, the population genetic structure of O ratosquilla oratoria from the Yellow Sea a... The mantis shrimp O ratosquilla oratoria is an ecologically and economically important species in the Western Pacific. In present study, the population genetic structure of O ratosquilla oratoria from the Yellow Sea and East China Sea was examined with mitochondrial DNA control region sequences. In total, 394 samples were collected from 18 locations and 102 haplotypes were obtained. For the Yellow Sea, the overall nucleotide diversity and haplotype diversity were 0.006 9 and 0.946 8, respectively; while across all the East China Sea locations, the overall nucleotide diversity and haplotype diversity were 0.027 94 and 0.979 0, respectively. The results of AMOVA and pairwise F_(ST)(0.145 2, P <0.001) revealed moderate differentiation between the Yellow Sea and East China Sea populations of O. oratoria. However, neither the neighbor-joining tree nor haplotype network showed clades with geographic pattern, which indicated considerable gene flow was existed between the Yellow Sea and East China Sea, and supporting the high larval dispersal ability in this species. Mismatch distribution analysis and neutrality tests suggested that O. oratoria has undergone population expansion event, and the Pleistocene glacial cycles might have an impact on the historical demography of O. oratoria. The genetic information obtained in this study can provide useful information for sustainable improvements for capture fisheries management strategies. 展开更多
关键词 Oratosquilla oratoria genetic structure mtDNA control region Yellow Sea East China Sea
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Complete Genomic Sequence of Transmissible Gastroenteritis Virus TS and 3' End Sequence Characterization Following Cell Culture 被引量:3
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作者 Jian-qiang LI Jie CHENG +8 位作者 Xi LAN Xue-rui LI Wei LI Xiang-ping YIN Bao-yu LI Bin YANG Zhi-yong LI Yun ZHANG Ji-xing LIU 《Virologica Sinica》 SCIE CAS CSCD 2010年第3期213-224,共12页
The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains. Phylogenetic... The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains. Phylogenetic tree analysis based on the amino acid and nucleotide sequences of the S gene showed that the TGEV strains were divided into 3 clusters. TGEV TS showed a close evolutionary relationship to the American Miller cluster but had a 5' non-translated region (NTR) sequence closely related to the American Purdue cluster. Continued culture in different cell types indicated that TGEV TS virulence could be attenuated after fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS. 展开更多
关键词 CLONING Complete genome Gastroenteritis virus (TGEV) Cell culture
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Complete Genome Sequence Analysis of Duck Circovirus Strains from Cherry Valley Duck 被引量:8
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作者 Xing-xiao Zhang Shao-ning Liu +2 位作者 Zhi-jing Xie Yi-bo Kong Shi-jin Jiang 《Virologica Sinica》 CAS CSCD 2012年第3期154-164,共11页
To investigate molecular epidemiology of DuCV in Cherry Valley ducks in China, the complete genomes of six DuCV strains, which were detected from Cherry Valley ducks in China between 2007 and 2008, were sequenced. Seq... To investigate molecular epidemiology of DuCV in Cherry Valley ducks in China, the complete genomes of six DuCV strains, which were detected from Cherry Valley ducks in China between 2007 and 2008, were sequenced. Sequence and phylogenetic analysis were carried out to compare these six strains with another 27 DuCV strains from Mulard duck, Muscovy duck, Pekin ducks and Mule duck. The analysis showed that the six DuCV strains exhibited typical genetic features of the family of DuCV, such as a stem-loop structure, three major open reading frames (Rep, Cap and ORF3), four intergenic repeats and the conserved motifs for rolling circle replication and for the dNTP binding domain located in the Rep protein. Phylogenetic analysis of the nucleotide sequences of the complete genome and Cap gene of these strains together with those that have been previously published demonstrated two distinct DuCV genotypes. The DuCV strains with complete genomes containing 1988 and 1989 nucleotides clustered in genotype A, whereas the strains with complete genomes containing 1991, 1992, 1995 and 1996 nucleotides lay in genotype B. The six DuCV strains from Cherry Valley ducks were divided into the two groups. The results of the study provides some insight into the variation of DuCVs in Cherry Valley ducks. 展开更多
关键词 Cherry Valley duck Duck circovirus Complete genome Phylogenetic analysis
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Molecular phylogeny of Bactrocera species (Diptera: Tephritidae) inferred from mitochondrial 16s rDNA sequences
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作者 ZHANG Bin LIU Ying-hong +1 位作者 WANG Ze-le ZHAO Lan-lan 《Journal of Agricultural Science and Technology》 2009年第6期24-28,共5页
The fruit flies of the Bactrocera (Diptera: Tephritidae) are one of the major economically important insects in Asia. So far, there are at least seven species found in Chongqing, China. By using mitochondrial 16s r... The fruit flies of the Bactrocera (Diptera: Tephritidae) are one of the major economically important insects in Asia. So far, there are at least seven species found in Chongqing, China. By using mitochondrial 16s rDNA sequences, the phylogenetic relationships among seven Bactrocera species, Bactrocera cucurbitae (Coquillett), B. tau (Walker), B. diaphora (Hendel), B. caudata (Fabricius), B. scutellata (Hendel), B. dorsalis (Hendel), and B. minax (Enderlein) were investigated. Nucleotide diversity within species ranged from 0.3% to 10.9%. According to the result, B. cucurbitae (Coquillett), B. tau (Walker), B. diaphora (Hendel), and B. caudata (Fabricius) have no enough sites to be distinguished among the other species in the 347bp nucleotide sequences of the 16s rDNA gene. At the same time, excluding B. scutellata (Hendel) and B. minax (Enderlein), the other species recognition sites are not only too little but also discontinuous. Phylogenetic trees calculated from both maximum parsimony and neighbor-joining phylogenetic analysis methods showed that B. cucurbitae and B. tau were closely related, B. diaphora (Hendel) and B. caudata (Fabricius) also had a close relationship. B. minax (Enderlein) has a furthest relationship from the other 6 species. From the results, we can infer that the our data supports previous classification of Bactrocera based on morphological characters in other words, but the Bactrocera subgenera, Zeugodacus is polyphyletic. 展开更多
关键词 Bactrocera spp 16s rDNA mitochondrial DNA molecular phylogeny
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Single Nucleotide Polymorphism Genotyping of Calpastatin Gene Using the ARMS Compared with the RFLP
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作者 P. Tavitchasri J. Sethakul +1 位作者 C. Kanthapanit W. Wajjwalku 《Journal of Agricultural Science and Technology(A)》 2011年第2X期164-169,共6页
Calpastatin is an endogenous inhibitor of calpain which is responsible for the breakdown of myofibrillar proteins, The association of Single Nucleotide Polymorphism (SNP) in the calpastatin gene with meat tenderness... Calpastatin is an endogenous inhibitor of calpain which is responsible for the breakdown of myofibrillar proteins, The association of Single Nucleotide Polymorphism (SNP) in the calpastatin gene with meat tenderness is an important topic in meat production. Therefore efficient procedure to investigate the SNP is necessary. The objectives of this study were to detect the SNP of calpastatin gene at domain L marker (G/C transversion) of the Kamphaengsaen beef breed (KPS cattle; n = 26) by the Amplification Refractory Mutation System (ARMS) compared with the Restriction Fragment Length Polymorphism (RFLP) methods and to determine the genotypes of the KPS cattle at that marker. Genomic DNA of calpastatin gene extracted from blood of the KPS cattle was detected with ARMS and RFLP methods. The ARMS system has utilized two primer pairs to amplify the two different alleles of a polymorphism in single PCR reaction to detected single base mutation. In this method, the alleles-specific primers had a mismatch at 3' terminal base and a second deliberate mismatch at position -2 from 3' terminus. While the RFLP method detected a polymorphism using PCR-base technique follow by RsaI restriction enzyme. Amplification of the ARMS method revealed that the results were not different from the conventional method of RFLP. Analysis of genotypes revealed that the KPS cattle inherited the CC, CG and GG genotypes at domain L marker. These were reliable when verified by nucleotide sequence analysis of PCR products. The animals were genotyped and determined for tenderness phenotype with this marker that predicted variation of an intronic polymorphism at domain L of the calpastatin gene. Therefore, the ARMS method was simple, efficient technique, and suitable for detecting SNP at domain L marker of the calpastatin gene. 展开更多
关键词 Single Nucleotide Polymorphism (SNP) Amplification Refractory Mutation System (ARMS) Restriction FragmentLength Polymorphism (RFLP) calpastatin gene meat tenderness.
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Identification of novel cyclic nucleotide phosphodiesterase gene cDNAs in the brain of guinea pig
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作者 龙跃生 黄敏齐 廖卫平 《Neuroscience Bulletin》 SCIE CAS CSCD 2010年第5期365-370,共6页
Objective Cyclic nucleotide phosphodiesterase(PDE)is a critical component of the nitric oxide(NO)signaling pathway and plays critical roles in cognition and learning,Parkinson’s disease,attention deficit hyperact... Objective Cyclic nucleotide phosphodiesterase(PDE)is a critical component of the nitric oxide(NO)signaling pathway and plays critical roles in cognition and learning,Parkinson’s disease,attention deficit hyperactivity disorder, psychosis and depression.The PDEs in the brain of guinea pig have not yet been reported.The present study aimed to detect the unknown Pde cDNAs in the brain of guinea pig.Methods Reverse transcription polymerase chain reaction(RT-PCR)and sequence comparison analysis were performed to detect the expression of Pde cDNAs and to assess the identity rates of cDNA and amino acid sequences between guinea pig and human or mouse,respectvely.The RT-PCR primers were located on the conserved region of human PDE and mouse Pde cDNAs.Results Eleven novel Pde cDNAs were detected in the brain of guinea pig(Cavia porcellus),including CpPde1a,CpPde1b,CpPde2a,CpPde4a,CpPde4d,CpPde5a,CpPde6c,CpPde7b, CpPde8a,CpPde9a,and CpPde10a.The identity rates of the Pde cDNA sequences between guinea pig and human ranged from 83.8%to 94.3%,and those of the amino acid sequences ranged from 91.9%to 100%.The identity rates of Pde cDNA sequences between guinea pig and mouse ranged from 84.6%to 92.1%,and those of amino acid sequences ranged from 91.2% to 99.2%.The average identity rate of the 11 Pde cDNA sequences between guinea pig and human was significantly higher(P 0.01)than that between guinea pig and mouse.The putative partial amino acid sequences of guinea pig contained at least one of the conserved domains of human and mouse PDE proteins.Conclusion These results indicate that the brainexpressed Pde genes are identified in guinea pig,which lays the foundation for further investigating the physiological roles of PDE proteins in the brain. 展开更多
关键词 cyclic nucleotide phosphodiesterase the central nervous system nitric oxide guinea pig cross-species comparison
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Binary sequences-based approach for construction of evolutionary network
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作者 Rinku Mathur 《International Journal of Biomathematics》 2014年第2期13-26,共14页
Evolutionary studies have been of prime importance to life scientists since ancient times. The advancements in technology has made it possible to make available the massive amounts of genomic data. The abundance of ge... Evolutionary studies have been of prime importance to life scientists since ancient times. The advancements in technology has made it possible to make available the massive amounts of genomic data. The abundance of genomic data poses new challenges for biologists, computer scientists and mathematicians to develop approaches for discovery of new relationships in data and evolutionary networks. In this work, nucleotide sequences are converted into binary sequences to explore the network among different species. A new approach based on binary sequences has been proposed to reconstruct the accurate phylogenetic network. The algorithm developed is validated by comparing the results with those obtained by already existing method of network construction. A program is also coded in C language on the Intel Core i3 Dell inspiron machine to obtain the evolutionary network. The new approach developed also provides the fast solutions as there is no need of aligning the sequences. 展开更多
关键词 Evolutionary network binary sequences hybrid nodes Hamming distance connected graph.
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