Objective. DNA modification fixed as mutations in the cells may be an essential factor in the initiation step of chemical carcinogenesis. In order to explore the mechanism of gene mutation...Objective. DNA modification fixed as mutations in the cells may be an essential factor in the initiation step of chemical carcinogenesis. In order to explore the mechanism of gene mutation and cell transformation induced by glycidyl methacrylate (GMA), the current test studied the characteristics of GMA DNA adducts formation in vitro. Methods. In vitro test, dAMP, dCMP, dGMP, dTMP and calf thymus DNA were allowed to react with GMA (Glycidyl Methacrylate). After the reaction, the mixtures were detected by UV and subjected to reversed phase HPLC on ultrasphere ODS reversed phase column, the reaction products were eluted with a linear gradients of methanol (solvent A) and 10mmol/L ammonium formate, pH5 0 (solvent B). The synthesized adducts were then characterized by UV spectroscopy in acid (pH1 0), neutral (pH7 2), alkaline (pH11 0) and by mass spectroscopy. Results. The results showed that GMA could bind with dAMP, dCMP, dGMP and calf thymus DNA by covalent bond, and the binding sites were specific (N 6 of adenine, N 3 of cytosine). Meanwhile, a main GMA DNA adduct in the reaction of GMA with calf thymus DNA was confirmed as N 3 methacrylate 2 hydroxypropy1 dCMP. Conclusions. GMA can react with DNA and /or deoxynucleotide monophosphate and generate some adducts such as N 6 methacrylate 2 hydroxypropyl dAMP and N 3 methacrylate 2 hydroxypropyl dCMP, ets. Formation of GMA DNA adducts is an important molecular event in gene mutation and cell transformation induced by GMA.展开更多
Two different kinds of experimental techniques were used to in-situ study the austenite formation during intercritical annealing in C-Mn dual phase steel. The microstructure evolution was observed by confocal laser sc...Two different kinds of experimental techniques were used to in-situ study the austenite formation during intercritical annealing in C-Mn dual phase steel. The microstructure evolution was observed by confocal laser scanning microscope, and the austenite isothermal and non-isothermal transformation kinetics were studied by dilatometry. The results indicate that banded structure is produced for the reason of composition segregation and the competition between recrystallization and phase transformation. Austenite prefers to nucleate not only at ferrite/ferrite grain boundaries, but also inside the grains of ferrite.Furthermore, the austenitizing process is accomplished mainly via migration of the existing austenite/ferrite interface rather than nucleation of new grains. The incubation process can be divided into two stages which are controlled by carbon and manganese diffusion, respectively. During the incubation process, the nucleation rate of austenite decreases, and austenite growth changes from two-dimensional to one-dimensional. The partitioning coefficient, defined as the ratio of manganese content in the austenite to that in the adjacent ferrite, increases with increasing soaking time.展开更多
With the development of radioactive-ion-beam facilities,many exotic phenomena have been discovered or predicted in the nuclei far from the stability line,including cluster structure,shell structure,deformed halo,and s...With the development of radioactive-ion-beam facilities,many exotic phenomena have been discovered or predicted in the nuclei far from the stability line,including cluster structure,shell structure,deformed halo,and shape decoupling effects.The study of exotic nuclear phenomena is at the frontier of nuclear physics nowadays.The covariant density functional theory(CDFT)is one of the most successful microscopic models in describing the structure of nuclei in almost the whole nuclear chart.Within the framework of CDFT,toward a proper treatment of deformation and weak binding,the deformed relativistic Hartree-Bogoliubov theory in continuum(DRHBc)has been developed.In this contribution,we review the applications and extensions of the DRHBc theory to the study of exotic nuclei.The DRHBc theory has been used to investigate the deformed halos in B,C,Ne,Na,and Mg isotopes and the theoretical descriptions are reasonably consistent with available data.A DRHBc Mass Table Collaboration has been founded,aiming at a high precision nuclear mass table with deformation and continuum effects included,which is underway.By implementing the angular momentum projection based on the DRHBc theory,the rotational excitations of deformed halos have been investigated and it is shown that the deformed halos and shape decoupling effects also exist in the low-lying rotational excitation states of deformed halo nuclei.展开更多
Using the total-Routhian-surface (TRS) method, the rotational behaviors of fission isomers in the second well of actinide nuclei 234-242U, 236-244pu and 238-246Cm were investigated. The pairing-deformation-frequency...Using the total-Routhian-surface (TRS) method, the rotational behaviors of fission isomers in the second well of actinide nuclei 234-242U, 236-244pu and 238-246Cm were investigated. The pairing-deformation-frequency self-consistent TRS calculations repro- duced reasonably the experimental moments of inertia extracted from spectroscopic data. It is calculated that, in these largely elongated (β2 ≈0.65 and β4≈ 0.03) fission isomers, the ν1/2-[981] neutron and π1/2+[651] proton align simultaneously at rotational frequency hω≈0.4 - 0.6 MeV (corresponding to spin I≈80h), which leads to clear upbending in moments of inertia (MoI's). Our calculations have indicated that the hexadecapole deformation f14 influenced significantly the frequency of the rotational alignment of the proton 1/2+[651] orbit.展开更多
Due to the super rotation of the Earth's inner core, the tilted figure axis of the inner core would progress with respect to the mantle and thus cause the variation of the Earth's external gravity field. This paper ...Due to the super rotation of the Earth's inner core, the tilted figure axis of the inner core would progress with respect to the mantle and thus cause the variation of the Earth's external gravity field. This paper improves the present model of the gravity field variation caused by the inner core super rotation. Under the assumption that the inner core is a stratifying ellipsoid whose density function is fitted out from PREM and the super rotation rate is 0.27-0.53°/yr, calculations show that the global temporal variations on the Earth's surface have a maximum value of about 0.79-1.54×10^3 pGal and a global average intensity of about 0.45-0.89×10^ 3 μGal in the whole year of 2007, which is beyond the accuracy of the present gravimetry and even the super conducting gravimeter data. However, both the gravity variations at Beijing and Wuhan vary like sine variables with maximal variations around 0.33 pGal and 0.29 pGal, respectively, in one cycle. Thus, continuous gravity measurements for one or two decades might be able to detect the differential motion of the inner core.展开更多
文摘Objective. DNA modification fixed as mutations in the cells may be an essential factor in the initiation step of chemical carcinogenesis. In order to explore the mechanism of gene mutation and cell transformation induced by glycidyl methacrylate (GMA), the current test studied the characteristics of GMA DNA adducts formation in vitro. Methods. In vitro test, dAMP, dCMP, dGMP, dTMP and calf thymus DNA were allowed to react with GMA (Glycidyl Methacrylate). After the reaction, the mixtures were detected by UV and subjected to reversed phase HPLC on ultrasphere ODS reversed phase column, the reaction products were eluted with a linear gradients of methanol (solvent A) and 10mmol/L ammonium formate, pH5 0 (solvent B). The synthesized adducts were then characterized by UV spectroscopy in acid (pH1 0), neutral (pH7 2), alkaline (pH11 0) and by mass spectroscopy. Results. The results showed that GMA could bind with dAMP, dCMP, dGMP and calf thymus DNA by covalent bond, and the binding sites were specific (N 6 of adenine, N 3 of cytosine). Meanwhile, a main GMA DNA adduct in the reaction of GMA with calf thymus DNA was confirmed as N 3 methacrylate 2 hydroxypropy1 dCMP. Conclusions. GMA can react with DNA and /or deoxynucleotide monophosphate and generate some adducts such as N 6 methacrylate 2 hydroxypropyl dAMP and N 3 methacrylate 2 hydroxypropyl dCMP, ets. Formation of GMA DNA adducts is an important molecular event in gene mutation and cell transformation induced by GMA.
基金Project(2013AA031601)supported by the National High Technology Research and Development Program of ChinaProject(2012BAF04B01)supported by the National Science and Technology Pillar Program During the 12th Five-year Plan Period of China
文摘Two different kinds of experimental techniques were used to in-situ study the austenite formation during intercritical annealing in C-Mn dual phase steel. The microstructure evolution was observed by confocal laser scanning microscope, and the austenite isothermal and non-isothermal transformation kinetics were studied by dilatometry. The results indicate that banded structure is produced for the reason of composition segregation and the competition between recrystallization and phase transformation. Austenite prefers to nucleate not only at ferrite/ferrite grain boundaries, but also inside the grains of ferrite.Furthermore, the austenitizing process is accomplished mainly via migration of the existing austenite/ferrite interface rather than nucleation of new grains. The incubation process can be divided into two stages which are controlled by carbon and manganese diffusion, respectively. During the incubation process, the nucleation rate of austenite decreases, and austenite growth changes from two-dimensional to one-dimensional. The partitioning coefficient, defined as the ratio of manganese content in the austenite to that in the adjacent ferrite, increases with increasing soaking time.
文摘With the development of radioactive-ion-beam facilities,many exotic phenomena have been discovered or predicted in the nuclei far from the stability line,including cluster structure,shell structure,deformed halo,and shape decoupling effects.The study of exotic nuclear phenomena is at the frontier of nuclear physics nowadays.The covariant density functional theory(CDFT)is one of the most successful microscopic models in describing the structure of nuclei in almost the whole nuclear chart.Within the framework of CDFT,toward a proper treatment of deformation and weak binding,the deformed relativistic Hartree-Bogoliubov theory in continuum(DRHBc)has been developed.In this contribution,we review the applications and extensions of the DRHBc theory to the study of exotic nuclei.The DRHBc theory has been used to investigate the deformed halos in B,C,Ne,Na,and Mg isotopes and the theoretical descriptions are reasonably consistent with available data.A DRHBc Mass Table Collaboration has been founded,aiming at a high precision nuclear mass table with deformation and continuum effects included,which is underway.By implementing the angular momentum projection based on the DRHBc theory,the rotational excitations of deformed halos have been investigated and it is shown that the deformed halos and shape decoupling effects also exist in the low-lying rotational excitation states of deformed halo nuclei.
基金supported by Zhong Xia Interscholastic Scientific Research Fundthe National Key Basic Research Program of China(Grant No.2013CB34400)the National Natural Science Foundation of China(Grant Nos.11235001,11375016,11320101004 and J1103206)
文摘Using the total-Routhian-surface (TRS) method, the rotational behaviors of fission isomers in the second well of actinide nuclei 234-242U, 236-244pu and 238-246Cm were investigated. The pairing-deformation-frequency self-consistent TRS calculations repro- duced reasonably the experimental moments of inertia extracted from spectroscopic data. It is calculated that, in these largely elongated (β2 ≈0.65 and β4≈ 0.03) fission isomers, the ν1/2-[981] neutron and π1/2+[651] proton align simultaneously at rotational frequency hω≈0.4 - 0.6 MeV (corresponding to spin I≈80h), which leads to clear upbending in moments of inertia (MoI's). Our calculations have indicated that the hexadecapole deformation f14 influenced significantly the frequency of the rotational alignment of the proton 1/2+[651] orbit.
基金Supported by the National Natural Science Foundation of China (No. 40574004 ,No. 40637034), the National 863 Program of China (No. 2006AA12Z211).Acknowledgement The authors are grateful to Prof. Dr. Buffet B A and Prof. Dr. Smylie D E, who provided very valuable comments and suggestions on our research work.
文摘Due to the super rotation of the Earth's inner core, the tilted figure axis of the inner core would progress with respect to the mantle and thus cause the variation of the Earth's external gravity field. This paper improves the present model of the gravity field variation caused by the inner core super rotation. Under the assumption that the inner core is a stratifying ellipsoid whose density function is fitted out from PREM and the super rotation rate is 0.27-0.53°/yr, calculations show that the global temporal variations on the Earth's surface have a maximum value of about 0.79-1.54×10^3 pGal and a global average intensity of about 0.45-0.89×10^ 3 μGal in the whole year of 2007, which is beyond the accuracy of the present gravimetry and even the super conducting gravimeter data. However, both the gravity variations at Beijing and Wuhan vary like sine variables with maximal variations around 0.33 pGal and 0.29 pGal, respectively, in one cycle. Thus, continuous gravity measurements for one or two decades might be able to detect the differential motion of the inner core.