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甲型H1N1流感病毒致宿主细胞脱氧核糖核酸氧化损伤的研究 被引量:1
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作者 郑君德 刘彦明 +5 位作者 刘汉欣 高月亭 陈涛 张丽梅 余琳 肖洪广 《中日友好医院学报》 2010年第5期289-291,F0004,共4页
目的:通过检测DNA损伤标志物8-氧鸟嘌呤脱氧核苷(8-oxo-dG)在甲型H1N1型流感病毒感染MDCK细胞中的表达,初步探讨甲型H1N1型流感病毒诱导细胞凋亡的脱氧核糖核酸(DNA)损伤机制。方法:MDCK细胞培养后,甲型H1N1流感病毒感染继续培养0h、1h... 目的:通过检测DNA损伤标志物8-氧鸟嘌呤脱氧核苷(8-oxo-dG)在甲型H1N1型流感病毒感染MDCK细胞中的表达,初步探讨甲型H1N1型流感病毒诱导细胞凋亡的脱氧核糖核酸(DNA)损伤机制。方法:MDCK细胞培养后,甲型H1N1流感病毒感染继续培养0h、1h、3h、6h、12h、24h、48h,细胞爬片免疫组织化学方法检测各时间点DNA损伤标志物8-oxo-dG的表达。结果:甲型H1N1流感病毒感染后各时间点实验组MDCK细胞8-oxo-dG的表达均显著高于相应的正常对照组(P<0.05),病毒感染各组间两两比较发现各组间差异显著。结论:甲型H1N1流感病毒感染能造成宿主细胞DNA氧化损伤。 展开更多
关键词 甲型H1N1流感病毒 脱氧核糖核酸氧化损伤 8-羟基脱氧鸟嘌呤
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室内生源性多环芳烃对DNA的氧化损伤 被引量:11
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作者 袭著革 晁福寰 +5 位作者 孙咏梅 李官贤 刘中文 张华山 杨丹凤 张伟 《中国公共卫生》 CAS CSCD 北大核心 2004年第9期1034-1036,共3页
目的 了解室内生活污染来源 -吸烟和烹调产生的多环芳烃 (PAHs)种类与含量 ,及其对DNA的氧化损伤作用。方法 采集室内烹调油烟和环境烟草烟雾颗粒物 ,应用气相色谱 -质谱 -选择性离子监测技术 (GC -MS-SIM )定量检测 10种PAHs,并用 1... 目的 了解室内生活污染来源 -吸烟和烹调产生的多环芳烃 (PAHs)种类与含量 ,及其对DNA的氧化损伤作用。方法 采集室内烹调油烟和环境烟草烟雾颗粒物 ,应用气相色谱 -质谱 -选择性离子监测技术 (GC -MS-SIM )定量检测 10种PAHs,并用 10种混合PAHs经气管灌注染毒大鼠 ,用液相色谱结合电化学检测技术测定肺组织DNA中产生的氧化损伤标志物 8-羟基脱氧鸟苷 (8-OHdG)。结果 PAHs广泛存在于烹调油烟冷凝物、油烟颗粒物以及环境烟草烟雾的主、侧流烟雾颗粒物之中。其标准混合物可诱导大鼠肺组织DNA氧化损伤形成 8-OHdG ,并呈现明确的剂量 -反应关系。 展开更多
关键词 环境烟草烟雾 烹调油烟 多环芳烃 核酸氧化损伤 8-羟基脱氧鸟苷
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Decreased mitochondrial deoxyribonucleic acid and increased oxidative damage in chronic hepatitis C 被引量:4
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作者 Hsu-Heng Yen Kai-Lun Shih +3 位作者 Ta-Tsung Lin Wei-Wen Su Maw-Soan Soon Chin-San Liu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第36期5084-5089,共6页
AIM: To determine whether alteration of the mito- chondria DNA (mtDNA) copy number and its oxidative damage index (mtDNA△CT) can be detected by analysis of peripheral blood cells in hepatitis C virus (HCV)- in... AIM: To determine whether alteration of the mito- chondria DNA (mtDNA) copy number and its oxidative damage index (mtDNA△CT) can be detected by analysis of peripheral blood cells in hepatitis C virus (HCV)- infected patients. METHODS: This study enrolled two groups of pa- tients aged 40-60 years: a control group and an HCV- infected group in Department of Gastroenterology and Hepatology in Changhua Christian Hospital. Patients with co-infection with hepatitis B virus or human im- munodeficiency virus, autoimmune disease, malignant neoplasia, pregnancy, thyroid disease, or alcohol con- sumption 〉 40 g/d were excluded. HCV-infected pa- tients who met the following criteria were included: (1) positive HCV antibodies for 〉 6 mo; (2) alanine aminotransferase (ALT) levels more than twice the upper lim- it of normal on at least two occasions during the past 6 mo; and (3) histological fibrosis stage higher than F1. The mtDNA copy number and oxidative damage index of HCV mtDNA (mtDNA△CT) were measured in periph- eral blood leukocytes. The association between mtDNA copy number and mtDNA△CT was further analyzed using clinical data. RESULTS: Forty-seven normal controls (male/female: 26/21, mean age 50.51 ± 6.15 years) and 132 HCV- infected patients (male/female: 76/61, mean age 51.65 ± 5.50 years) were included in the study. The geno- types of HCV-infected patients include type 1a (n = 3), type 1b (n = 83), type 2a (n = 32), and type 2b (n = 14). Liver fibrosis stages were distributed as follows: F1/F2/F3/F4 = 1/61/45/25 and activity scores were A0/ A1/A2/A3 = 7/45/55/25. There were no age or gender differences between the two groups. HCV-infected pa- tients had higher hepatitis activity (aspartate transami- nase levels 108.77 ± 60.73 vs 23.19 ± 5.47, P 〈 0.01; ALT levels 168.69 ± 93.12 vs 23.15 ± 9.45, P 〈 0.01) and lower platelet count (170.40±58.00 vs 251.24 ± 63.42, P 〈 0.01) than controls. The mtDNA copy num- ber was lower in HCV-infected patients than in controls (173.49 vs 247.93, P 〈 0.05). The mtDNA△CT was higher in HCV-infected patients than in controls (2.92 vs 0.64, P 〈 0.05). To clarify the clinical significance of these results in HCV-infected patients, their association with different clinical parameters among HCV-infected pa- tients was analyzed. A negative association was found between mtDNA copy number and elevated aspartate transaminase levels (r = -0.17, P 〈 0.05). Changes in mtDNA copy number were not associated with HCV RNA levels, HCV genotypes, liver fibrosis severity, or inflammatory activity in the liver biopsy specimen. How- ever, a correlation was observed between mtDNA△CT and platelet count (r = -0.22, P 〈 0.01), HCV RNA level (r = 0.36, P 〈 0.01), and hepatitis activity (r = 0.20, P = 0.02). However, no difference in the change in mtDNA△CT was observed between different fibrosis stages or HCV CONCLUSION: Oxidative stress and mtDNA dam- age are detectable in patient's peripheral leukocytes. Increased leukocyte mtDNA△CT correlates with higher HCV viremia, increased hepatitis activity, and lower platelet count. 展开更多
关键词 Hepatitis C MITOCHONDRIA Oxidative stress Mitochondrial DNA BIOMARKER
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