乳腺癌穿刺组织与根治组织免疫组化对比分析

目的:研究乳腺癌穿刺活检组织ER、PR、HER2、Ki-67、Pgp及Gstπ免疫组化检测准确性。方法:选取74例乳腺癌根治术病例(均未进行过新辅内分泌治疗),比较术前穿刺活检和术后切除标本的ER、PR、HER2、Ki-67、Pgp及Gstπ免疫组化结果。结果:术前穿刺活检与手术标本ER的一致性为82.4%、PR的一致性为78.3%、HER2的一致性为75.6%、Ki-67的一致性为82.4%、Pgp的一致性为86.4%、Gstπ的一致性为94.5%。结论:穿刺标本与手术切除标本的免疫组化状态有一定的不致性。因此,在选择治疗方案时应进行综合考虑,而术前穿刺组织行免疫组化是有必要的。

探讨口腔癌根治同期组织瓣修复患者术后口腔护理方法

目的:探讨口腔癌根治同期组织瓣修复患者术后口腔护理方法。方法:选择2017年12月—2018年11月进行口腔癌根治同期组织瓣修复的患者94例作为观察对象,按随机数字表法将患者分为对照组(n=47)和观察组(n=47)。对照组患者采用常规的口腔护理方法,观察组患者在其基础上进行优质护理干预。比较两组患者护理前与护理后5d、10d、15d口腔清洁情况,对患者的护理满意度进行评分。结果:护理前两组患者口腔清洁情况评分比较无统计学意义(P>0.05),护理后5d、10d、15d观察组患者口腔清洁情况评分低于对照组(P<0.05)。经过15d护理后,观察组患者对医护人员的操作技巧、技术专业性以及服务态度方面的评分显著高于对照组(P<0.05)。结论:对口腔癌根治同期组织瓣修复患者在常规护理基础上进行优质的口腔护理,能够加强患者口腔的清洁,帮助患者尽快回归到日常生活之中,值得推广应用。

Effect of puerarin on matrix metalloproteinase-2 in human fetal scleral fibroblasts treated with low frequency electromagnetic fields

OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).METHODS:Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h.The experimental groups were divided into subgroups treated with 0,0.1,1and 10μM puerarin respectively.The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS:MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h.Puerarin at the concentration of 0.1μM reversed this effect by 8.53%in mRNA and by 17.97%in protein expression(P<0.05).The effect was more prominent at higher concentrations(1 and 10μM,P<0.01).CONCLUSION:Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells.Puerarin reversed the action to some extent in a specific concentration range.Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.