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Leukamenin E调节拟南芥幼苗根部生长素极性运输及根系生长发育 被引量:6
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作者 何静 丁兰 +1 位作者 李芃 郇兆蔚 《生态学杂志》 CAS CSCD 北大核心 2019年第10期2959-2967,共9页
本文采用14种拟南芥转基因报告株系,研究了对映-贝壳杉烷二萜(leukamenin E)调节拟南芥根部生长素极性运输转运蛋白表达并影响根生长发育的机制。结果表明:leukamenin E浓度在20~40μmol·L-1范围显著抑制拟南芥幼苗主根的生长,较... 本文采用14种拟南芥转基因报告株系,研究了对映-贝壳杉烷二萜(leukamenin E)调节拟南芥根部生长素极性运输转运蛋白表达并影响根生长发育的机制。结果表明:leukamenin E浓度在20~40μmol·L-1范围显著抑制拟南芥幼苗主根的生长,较低浓度leukamenin E(10μmol·L-1)促进侧根提前发生并增加侧根数量;10~30μmol·L-1的leukamenin E在处理拟南芥幼苗48 h后可显著提高其根尖部生长素水平;进一步检测根部生长素极性运输转运蛋白表达,发现该浓度条件下leukamenin E促进PIN1在转录水平表达并增加其蛋白丰度,但在转录水平抑制PIN2表达并减少其蛋白丰度;同时,显著减少PIN3、PIN7和PIN4蛋白在小柱和静止中心及其周围细胞的丰度以及AUX1的定位丰度;Leukamenin E对PIN1丰度的上调以及对PIN2、PIN3、PIN4、PIN7和AUX1定位丰度的减少可导致生长素在根尖顶部的积累以及根部生长素浓度梯度的改变,引起根生长的抑制及侧根的提前发生。 展开更多
关键词 对映-贝壳杉烷二萜 生长 生长素极性运输载体 根生长调节
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Effect of Growth Regulators on Rooting of Hardwood Cuttings of Lycium shawii, Nitraria retusa and Farsetia aegyptia 被引量:2
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作者 M. K. Suleiman N. R. Bhat S. Jacob R. R. Thomas 《Journal of Agricultural Science and Technology(B)》 2011年第3期414-419,共6页
Utilization of native plants in urban landscaping can potentially support their conservation and safeguard endangered native species from extinction. In this study, efforts were made to standardize vegetative propagat... Utilization of native plants in urban landscaping can potentially support their conservation and safeguard endangered native species from extinction. In this study, efforts were made to standardize vegetative propagation techniques for Lycium shawiL Nitraria retusa and Farsetia aegyptia. Effects of varying concentrations of rooting hormones on the success of rooting of hardwood cuttings of these species were studied in this research. Rooting in hardwood cuttings of Lycium shawii was enhanced (100%) by treating them with 1,000 ppm IBA, Ormone radicante in polvere (5,000 ppm NAA) and hormex (1,000 ppm IBA). Treatment with 1,000 ppm NAA was found to be effective for Nitraria retusa, while marginal success was recorded in rooting of hardwood cuttings of Farsetia aegyptia when treated with 400 ppm NAA. Rooting in untreated cuttings was 80%. 展开更多
关键词 Urban landscape vegetative propagation species conservation growth hormones NAA IBA and GA3.
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