The paper studies the effect of incubation of rhizobia with soybean seeds lectin on formation and functioning of alfalafa-Sinorhizobium meliloti symbiosis under the optimal water supply and drought conditions. It was ...The paper studies the effect of incubation of rhizobia with soybean seeds lectin on formation and functioning of alfalafa-Sinorhizobium meliloti symbiosis under the optimal water supply and drought conditions. It was shown that the addition of lectin to the inoculation compositions intensifies physiological processes in alfalfa plants： increases nitrogen fixing activity of symbiosis, nodule number on roots, as well as chlorophyll and carotenoid content in leaves, enhances plant growth during budding-fruiting period and reduces the negative effect of drought on alfalfa productivity.展开更多
To identify the genes involved in nodule formation and to increase usable molecular probes, a cDNA library of Astragalus sinicus genes specifically expressed in infected roots by Mesorhizobium huakuii 7653R is generat...To identify the genes involved in nodule formation and to increase usable molecular probes, a cDNA library of Astragalus sinicus genes specifically expressed in infected roots by Mesorhizobium huakuii 7653R is generated using a PCR-based suppressive subtractive hybridization (SSH) technique with two mRNA populations of infected and uninfected control roots. Two nodule-specific genes, AsIIC259 and AsG2511, are identified from infected roots of A. sinicus. The amino acid sequences deduced from the open reading frames (ORFs) reveal that AsIIC259 and AsG2511 encodes a polypeptide with 134 and 58 amino acids, respectively. A signal peptide sequence is predicted with high probability at the N-termini of the AsIIC259 and AsG2511. The motif searches show that the deduced polypeptide of AsIIC259 con- tains two N-glycosylation sites, a cAMP- and cGMP-dependent protein kinase phosphorylation site and a casein kinase II phosphorylation site. BLASTP searches reveal that AsIIC259 putative protein displays a low degree of similarity to a unique nodulin from Lupinus luteus nodules. No significant identity is displayed over the predicted polypeptides of AsG2511 with any published sequences. Virtual Northern blot and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses indicate that the two genes are expressed exclusively in inoculated roots and that their expression is 2―4 d later than that of the leghaemoglobin (Lb) gene during nodule development.展开更多
Plants are frequently exposed to adverse environments during their life span.Among them drought stress is one of the major threats to agricultural productivity.In order to survive in such unstable environment,plants h...Plants are frequently exposed to adverse environments during their life span.Among them drought stress is one of the major threats to agricultural productivity.In order to survive in such unstable environment,plants have developed mechanisms through which they recognize the severity of the stress based on the incoming environmental stimuli.To combat the detrimental effects of drought,the plants have evolved various strategies to modulate their physio-hormonal attributes.These strategies that can be modulated by shade and microbes contribute to enhancing tolerance to drought and reducing yield loss.Plant hormones,such as abscisic acid,auxin and ethylene have a major role in the shade-and microbe-associated improvement of drought tolerance through their effects on various metabolic pathways.In this process,the CLAVATA3/EMBRYOSURROUNDING REGION-RELATED 25 peptide has a major role due to its effect on ABA synthesis as shown in our regulatory model.展开更多
文摘The paper studies the effect of incubation of rhizobia with soybean seeds lectin on formation and functioning of alfalafa-Sinorhizobium meliloti symbiosis under the optimal water supply and drought conditions. It was shown that the addition of lectin to the inoculation compositions intensifies physiological processes in alfalfa plants： increases nitrogen fixing activity of symbiosis, nodule number on roots, as well as chlorophyll and carotenoid content in leaves, enhances plant growth during budding-fruiting period and reduces the negative effect of drought on alfalfa productivity.
基金Supported by the National Basic Research Program of China (Grant No. 2001CB108901)
文摘To identify the genes involved in nodule formation and to increase usable molecular probes, a cDNA library of Astragalus sinicus genes specifically expressed in infected roots by Mesorhizobium huakuii 7653R is generated using a PCR-based suppressive subtractive hybridization (SSH) technique with two mRNA populations of infected and uninfected control roots. Two nodule-specific genes, AsIIC259 and AsG2511, are identified from infected roots of A. sinicus. The amino acid sequences deduced from the open reading frames (ORFs) reveal that AsIIC259 and AsG2511 encodes a polypeptide with 134 and 58 amino acids, respectively. A signal peptide sequence is predicted with high probability at the N-termini of the AsIIC259 and AsG2511. The motif searches show that the deduced polypeptide of AsIIC259 con- tains two N-glycosylation sites, a cAMP- and cGMP-dependent protein kinase phosphorylation site and a casein kinase II phosphorylation site. BLASTP searches reveal that AsIIC259 putative protein displays a low degree of similarity to a unique nodulin from Lupinus luteus nodules. No significant identity is displayed over the predicted polypeptides of AsG2511 with any published sequences. Virtual Northern blot and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses indicate that the two genes are expressed exclusively in inoculated roots and that their expression is 2―4 d later than that of the leghaemoglobin (Lb) gene during nodule development.
基金This work was supported by the National Research,Development and Innovation Office(grant no.K131638)National Natural Science Foundation of China(grant nos.31871552,31671445)Sichuan Science and Technology Program(grant no.2018HH0108).
文摘Plants are frequently exposed to adverse environments during their life span.Among them drought stress is one of the major threats to agricultural productivity.In order to survive in such unstable environment,plants have developed mechanisms through which they recognize the severity of the stress based on the incoming environmental stimuli.To combat the detrimental effects of drought,the plants have evolved various strategies to modulate their physio-hormonal attributes.These strategies that can be modulated by shade and microbes contribute to enhancing tolerance to drought and reducing yield loss.Plant hormones,such as abscisic acid,auxin and ethylene have a major role in the shade-and microbe-associated improvement of drought tolerance through their effects on various metabolic pathways.In this process,the CLAVATA3/EMBRYOSURROUNDING REGION-RELATED 25 peptide has a major role due to its effect on ABA synthesis as shown in our regulatory model.
