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外源ABA对拟南芥根系皮层细胞水力学特征的影响 被引量:1
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作者 张静媛 慕自新 张岁岐 《植物生理学报》 CAS CSCD 北大核心 2013年第3期264-272,共9页
在溶液培养条件下,以拟南芥野生型哥伦比亚(Col)、兰兹伯格(Ler)和ABA胞质受体PYR/PYL/RCAR四突变体pyr1;pyl1;pyl2;pyl4(Qm)为试验材料,利用细胞压力探针技术对不同浓度外源ABA和不同时间处理下的根系皮层细胞的细胞膨压(P)、水分跨膜... 在溶液培养条件下,以拟南芥野生型哥伦比亚(Col)、兰兹伯格(Ler)和ABA胞质受体PYR/PYL/RCAR四突变体pyr1;pyl1;pyl2;pyl4(Qm)为试验材料,利用细胞压力探针技术对不同浓度外源ABA和不同时间处理下的根系皮层细胞的细胞膨压(P)、水分跨膜运输半时间(TW1/2)、细胞壁体积弹性模量(ε)以及细胞水导(Lp)进行测定,从分子遗传学水平探讨ABA影响拟南芥根系水力学特性的早期信号。结果表明,外源ABA处理使野生型Col和Ler根系皮层细胞的水力学性质产生不同的变化,其中,细胞膨压(P)在处理前后无变化;细胞壁弹性模量(ε)随ABA浓度及ABA处理时间变化而发生变化,但变化趋势不明显;而外源ABA处理使TW1/2和Lp发生明显变化,即TW1/2随ABA浓度升高而降低,对于同一浓度处理而言,TW1/2则随ABA处理时间的延长先降低后增加,各浓度在处理60min时均降到最低值,即TW1/2在1000nmol·L-1下处理60min达到最低值,而在处理120min时TW1/2又恢复,趋近于对照的值;相反Lp在1000nmol·L-1下处理60min时达到最大值,处理120min时恢复到对照水平,即表现出瞬时效应。与野生型Col和Ler相比,Qm的TW1/2及Lp均对外源ABA不敏感。说明ABA提高了拟南芥根系皮层细胞的透水作用,而且ABA受体PYR/PYL/RCAR介导的信号转导参与了ABA的水分调节效应。 展开更多
关键词 外源ABA 拟南芥 细胞压力探针 根皮层细胞 水力学参数
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Cell Cycle Kinetic Analysis in the Cortical Regions of the Lentil Primary Root During Germination 被引量:1
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作者 喻富根 庞延军 +1 位作者 赵剑春 王强 《Acta Botanica Sinica》 CSCD 2002年第7期764-770,共7页
Cell cycle kinetic activity in the cortical cells of the lentil (Lens culinaris Me-die. cv. Verte du Puy) primary root during germination was examined both temporally and spatially. Immunohistochemical and cytological... Cell cycle kinetic activity in the cortical cells of the lentil (Lens culinaris Me-die. cv. Verte du Puy) primary root during germination was examined both temporally and spatially. Immunohistochemical and cytological evidence indicated that DNA replication and cell division started in the cortical cells of tire lentil primary root after around 13 and 17 h of imbibition, respectively. The first cells in DNA synthesis and the First mitotic figures all appeared in the cortical cells about I mm front the root-cap junction, but these divided cells had synthesized their DNA during the maturity of seed instead of during germination. The kinetic pattern of activity of the first cell cycle showed that these cells were not activated synchronously, but re-entered the cell cycle in turn depending on their places in the root tip, However, the adjacent cells partially synchronously proceeded their cell cycle. 展开更多
关键词 cell cycle primary root GERMINATION IMMUNOHISTOCHEMISTRY Lens culinaris
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